{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,17]],"date-time":"2026-03-17T12:41:12Z","timestamp":1773751272129,"version":"3.50.1"},"reference-count":21,"publisher":"Elsevier BV","issue":"4","license":[{"start":{"date-parts":[[2013,8,1]],"date-time":"2013-08-01T00:00:00Z","timestamp":1375315200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2013,8,1]],"date-time":"2013-08-01T00:00:00Z","timestamp":1375315200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2016,2,25]],"date-time":"2016-02-25T00:00:00Z","timestamp":1456358400000},"content-version":"vor","delay-in-days":938,"URL":"http:\/\/www.elsevier.com\/open-access\/userlicense\/1.0\/"}],"funder":[{"name":"Department of Immunology, Faculty of Medicine, Kurdistan University of Medical Sciences, Sanandaj, Iran"}],"content-domain":{"domain":["clinicalkey.com","clinicalkey.com.au","clinicalkey.es","clinicalkey.fr","clinicalkey.jp","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Journal of Microbiology, Immunology and Infection"],"published-print":{"date-parts":[[2013,8]]},"DOI":"10.1016\/j.jmii.2012.03.011","type":"journal-article","created":{"date-parts":[[2012,6,22]],"date-time":"2012-06-22T20:32:09Z","timestamp":1340397129000},"page":"253-258","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":17,"title":["Induction of protective immunity against brucellosis in mice by vaccination with a combination of naloxone, alum, and heat-killed Brucella melitensis 16 M"],"prefix":"10.1016","volume":"46","author":[{"given":"Yousef","family":"Motaharinia","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mohammad Ali","family":"Rezaee","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ahmad","family":"Rashidi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ali","family":"Jalili","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mohammad Jafar","family":"Rezaie","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Reza","family":"Shapouri","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Werya","family":"Hossieni","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mohammad Reza","family":"Rahmani","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1016\/j.jmii.2012.03.011_bib1","doi-asserted-by":"crossref","first-page":"2827","DOI":"10.1016\/j.vaccine.2004.01.001","article-title":"Development and evaluation as vaccines in mice of Brucella melitensis Rev.1 single and double deletion mutants of the bp26 and omp31 genescoding for antigens of diagnostic significance in ovine brucellosis","volume":"22","author":"Cloeckaert","year":"2004","journal-title":"Vaccine"},{"key":"10.1016\/j.jmii.2012.03.011_bib2","doi-asserted-by":"crossref","first-page":"5877","DOI":"10.1128\/IAI.67.11.5877-5884.1999","article-title":"Protection of mice against brucellosis by vaccination with Brucella melitensis WR201(16MDpurEK)","volume":"67","author":"Hoover","year":"1999","journal-title":"Infect Immun"},{"key":"10.1016\/j.jmii.2012.03.011_bib3","doi-asserted-by":"crossref","first-page":"511","DOI":"10.1046\/j.1365-2567.2001.01258.x","article-title":"Interferon-\u03b3 is crucial from surviving a Brucella abortus infection in both resistant C57BL\/6 and susceptible BALB\/c mice","volume":"103","author":"Murphy","year":"2001","journal-title":"Immunol"},{"key":"10.1016\/j.jmii.2012.03.011_bib4","doi-asserted-by":"crossref","first-page":"969","DOI":"10.1128\/iai.63.3.969-975.1995","article-title":"Differential activation of Brucella reactive CD4+ T cells by Brucella infection or immunization with antigenic extracts","volume":"63","author":"Zhan","year":"1995","journal-title":"Infect Immun"},{"key":"10.1016\/j.jmii.2012.03.011_bib5","doi-asserted-by":"crossref","first-page":"134","DOI":"10.1016\/j.micinf.2010.10.015","article-title":"Cell-mediated immunity in human brucellosis","volume":"13","author":"Skendros","year":"2011","journal-title":"Microb and Infect"},{"key":"10.1016\/j.jmii.2012.03.011_bib6","doi-asserted-by":"crossref","first-page":"227","DOI":"10.1016\/j.clim.2004.07.010","article-title":"Cellular bioterrorism: how Brucella corrupts macrophage physiology to promote invasion and proliferation","volume":"114","author":"Maria-Pilara","year":"2005","journal-title":"Clin Immunol"},{"key":"10.1016\/j.jmii.2012.03.011_bib7","doi-asserted-by":"crossref","first-page":"249","DOI":"10.1007\/978-1-4615-1823-5_9","article-title":"Structure and properties of aluminum-containing adjuvants","volume":"6","author":"Hem","year":"1995","journal-title":"Pharm Biotechnol"},{"key":"10.1016\/j.jmii.2012.03.011_bib8","doi-asserted-by":"crossref","first-page":"217","DOI":"10.1093\/intimm\/dxn139","article-title":"A novel adjuvant, the general opioid antagonist naloxone, elicits a robust cellular immune response for a DNA vaccine","volume":"21","author":"Jamali","year":"2009","journal-title":"Internation Immunol"},{"key":"10.1016\/j.jmii.2012.03.011_bib9","doi-asserted-by":"crossref","first-page":"382","DOI":"10.1016\/j.micinf.2010.02.001","article-title":"Evaluation of the adjuvant activity of naloxone, an opioid receptor antagonist, in combination with heat-killed Listeria monocytogenes vaccine","volume":"12","author":"Jazani","year":"2010","journal-title":"Microb and Infect"},{"key":"10.1016\/j.jmii.2012.03.011_bib10","doi-asserted-by":"crossref","first-page":"5181","DOI":"10.1128\/IAI.00231-09","article-title":"Nondividing but metabolically active gamma-irradiated Brucella melitensis is protective against virulent B. melitensis challenge in mice","volume":"77","author":"Magnani","year":"2009","journal-title":"Infect Immun"},{"key":"10.1016\/j.jmii.2012.03.011_bib11","doi-asserted-by":"crossref","first-page":"54","DOI":"10.1111\/j.1574-695X.2010.00747.x","article-title":"A novel adjuvant, a mixture of alum and the general opioid antagonist naloxone, elicits both humoral and cellular immune responses for heat-killed Salmonella typhimurium vaccine","volume":"61","author":"Jazani","year":"2011","journal-title":"FEMS Immunol Med Microbiol"},{"key":"10.1016\/j.jmii.2012.03.011_bib12","doi-asserted-by":"crossref","first-page":"5526","DOI":"10.1016\/j.vaccine.2011.05.030","article-title":"Immunisation with the glycolytic enzyme enolase confers effective protection against Candida albicans infection in mice","volume":"29","author":"Li","year":"2011","journal-title":"Vaccine"},{"key":"10.1016\/j.jmii.2012.03.011_bib13","first-page":"15","article-title":"Evaluation of real-time PCR method for rapid diagnosis of brucellosis with different clinical manifestations","volume":"58","author":"Surucouglu","year":"2009","journal-title":"Polish J Microbiol"},{"key":"10.1016\/j.jmii.2012.03.011_bib14","doi-asserted-by":"crossref","first-page":"156","DOI":"10.1016\/j.vetmic.2008.12.023","article-title":"Real-time PCR for identification of Brucella spp.: a comparative study of IS711, bcsp31 and per target genes","volume":"137","author":"Bounaadja","year":"2009","journal-title":"Vet Microbiol"},{"key":"10.1016\/j.jmii.2012.03.011_bib15","doi-asserted-by":"crossref","first-page":"109","DOI":"10.1007\/s10096-007-0409-y","article-title":"Comparison of seven commercial DNA extraction kits for the recovery of Brucella DNA from spiked human serum samples using real-time PCR","volume":"27","author":"Queipo-Ortu\u00f1o","year":"2008","journal-title":"Eur J Clin Microbiol Infect Dis"},{"key":"10.1016\/j.jmii.2012.03.011_bib16","doi-asserted-by":"crossref","first-page":"7004","DOI":"10.1128\/IAI.70.12.7004-7012.2002","article-title":"Splitter GA.Virulence criteria for Brucella abortus strains as determined by interferon regulatory factor 1-deficient mice","volume":"70","author":"Ko","year":"2002","journal-title":"Infect Immun"},{"key":"10.1016\/j.jmii.2012.03.011_bib17","doi-asserted-by":"crossref","first-page":"354","DOI":"10.1016\/j.vetmic.2011.03.031","article-title":"The 16MDvjbR as an ideal live attenuated vaccine candidate for differentiation between vaccination and infection","volume":"151","author":"Wang","year":"2011","journal-title":"Vet Microbiol"},{"key":"10.1016\/j.jmii.2012.03.011_bib18","doi-asserted-by":"crossref","first-page":"1766","DOI":"10.1016\/j.vaccine.2009.12.012","article-title":"Protection of BALB\/c mice against Brucella melitensis 16M infection induced by vaccination with live Escherichia coli expression Brucella P39 protein","volume":"28","author":"Al-Mariri","year":"2010","journal-title":"Vaccine"},{"issue":"S2","key":"10.1016\/j.jmii.2012.03.011_bib19","first-page":"S2\/26","article-title":"Novel human polysaccharide adjuvants with dual Th1 and Th2 potentiating activity","volume":"24","author":"Petrovsky","year":"2006","journal-title":"Vaccine"},{"key":"10.1016\/j.jmii.2012.03.011_bib20","doi-asserted-by":"crossref","first-page":"4913","DOI":"10.1016\/j.vaccine.2008.07.030","article-title":"Evaluation of Brucella melitensis B115 as rough-phenotype vaccine against B. melitensis and B. ovis infections","volume":"26","author":"Adone","year":"2008","journal-title":"Vaccine"},{"key":"10.1016\/j.jmii.2012.03.011_bib21","doi-asserted-by":"crossref","first-page":"1958","DOI":"10.1128\/JCM.37.6.1958-1963.1999","article-title":"Quantitative detection of Borrelia burgdorferi by real-time PCR","volume":"37","author":"Pah","year":"1999","journal-title":"J Clin Microbiol"}],"container-title":["Journal of Microbiology, Immunology and Infection"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1684118212000497?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1684118212000497?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,10,28]],"date-time":"2025-10-28T13:17:18Z","timestamp":1761657438000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S1684118212000497"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2013,8]]},"references-count":21,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2013,8]]}},"alternative-id":["S1684118212000497"],"URL":"https:\/\/doi.org\/10.1016\/j.jmii.2012.03.011","relation":{},"ISSN":["1684-1182"],"issn-type":[{"value":"1684-1182","type":"print"}],"subject":[],"published":{"date-parts":[[2013,8]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Induction of protective immunity against brucellosis in mice by vaccination with a combination of naloxone, alum, and heat-killed Brucella melitensis 16 M","name":"articletitle","label":"Article Title"},{"value":"Journal of Microbiology, Immunology and Infection","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.jmii.2012.03.011","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"Copyright \u00a9 2012 Published by Elsevier Taiwan LLC.","name":"copyright","label":"Copyright"}]}}