{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,30]],"date-time":"2026-10-30T04:04:07Z","timestamp":1793333047083,"version":"4.5.0"},"reference-count":46,"publisher":"Elsevier BV","issue":"1","license":[{"start":{"date-parts":[[2026,2,1]],"date-time":"2026-02-01T00:00:00Z","timestamp":1769904000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2026,2,1]],"date-time":"2026-02-01T00:00:00Z","timestamp":1769904000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2025,7,11]],"date-time":"2025-07-11T00:00:00Z","timestamp":1752192000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100012669","name":"Natural Science Foundation Project of Chongqing","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100012669","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100005230","name":"Natural Science Foundation of Chongqing Municipality","doi-asserted-by":"publisher","award":["CSTB2024NSCQ-KJFZZDX0034"],"award-info":[{"award-number":["CSTB2024NSCQ-KJFZZDX0034"]}],"id":[{"id":"10.13039\/501100005230","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["clinicalkey.com","clinicalkey.com.au","clinicalkey.es","clinicalkey.fr","clinicalkey.jp","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Journal of Microbiology, Immunology and Infection"],"published-print":{"date-parts":[[2026,2]]},"DOI":"10.1016\/j.jmii.2025.07.006","type":"journal-article","created":{"date-parts":[[2025,7,15]],"date-time":"2025-07-15T19:49:01Z","timestamp":1752608941000},"page":"71-84","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":1,"title":["The novel pyridine-fused quinolinone PQ-L5 exhibits antibacterial effects and enhances \u03b2-lactam activity by targeting PBP2a in methicillin-resistant Staphylococcus aureus"],"prefix":"10.1016","volume":"59","author":[{"given":"Wei","family":"Feng","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qian","family":"Yuan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaowen","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qianmei","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ye","family":"Wei","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2679-8797","authenticated-orcid":false,"given":"Fengjun","family":"Sun","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.jmii.2025.07.006_bib1","doi-asserted-by":"crossref","first-page":"629","DOI":"10.1016\/S0140-6736(21)02724-0","article-title":"Global burden of bacterial antimicrobial resistance in 2019: a systematic analysis","volume":"399","author":"Antimicrobial Resistance","year":"2022","journal-title":"Lancet"},{"key":"10.1016\/j.jmii.2025.07.006_bib2","doi-asserted-by":"crossref","first-page":"603","DOI":"10.1128\/CMR.00134-14","article-title":"Staphylococcus aureus infections: epidemiology, pathophysiology, clinical manifestations, and management","volume":"28","author":"Tong","year":"2015","journal-title":"Clin Microbiol Rev"},{"key":"10.1016\/j.jmii.2025.07.006_bib3","series-title":"WHO Bacterial Priority Pathogens List, 2024: Bacterial Pathogens of Public Health Importance to Guide Research, Development and Strategies to Prevent and Control Antimicrobial Resistance","year":"2024"},{"key":"10.1016\/j.jmii.2025.07.006_bib4","doi-asserted-by":"crossref","first-page":"169","DOI":"10.1016\/j.jare.2019.10.005","article-title":"Vancomycin resistant Staphylococcus aureus infections: a review of case updating and clinical features","volume":"21","author":"Cong","year":"2020","journal-title":"J Adv Res"},{"key":"10.1016\/j.jmii.2025.07.006_bib5","doi-asserted-by":"crossref","DOI":"10.1128\/MMBR.00026-19","article-title":"Staphylococcal biofilm development: structure, regulation, and treatment strategies","volume":"84","author":"Schilcher","year":"2020","journal-title":"Microbiol Mol Biol Rev"},{"key":"10.1016\/j.jmii.2025.07.006_bib6","doi-asserted-by":"crossref","DOI":"10.1128\/cmr.00221-20","article-title":"Environmental, microbiological, and immunological features of bacterial biofilms associated with implanted medical devices","volume":"35","author":"Caldara","year":"2022","journal-title":"Clin Microbiol Rev"},{"key":"10.1016\/j.jmii.2025.07.006_bib7","doi-asserted-by":"crossref","first-page":"397","DOI":"10.1038\/s41579-018-0019-y","article-title":"Implant infections: adhesion, biofilm formation and immune evasion","volume":"16","author":"Arciola","year":"2018","journal-title":"Nat Rev Microbiol"},{"key":"10.1016\/j.jmii.2025.07.006_bib8","doi-asserted-by":"crossref","first-page":"276","DOI":"10.1093\/femsre\/fux010","article-title":"Molecular mechanisms of biofilm-based antibiotic resistance and tolerance in pathogenic bacteria","volume":"41","author":"Hall","year":"2017","journal-title":"FEMS Microbiol Rev"},{"key":"10.1016\/j.jmii.2025.07.006_bib9","doi-asserted-by":"crossref","first-page":"522","DOI":"10.1080\/21505594.2017.1313372","article-title":"Strategies for combating bacterial biofilms: a focus on anti-biofilm agents and their mechanisms of action","volume":"9","author":"Roy","year":"2018","journal-title":"Virulence"},{"key":"10.1016\/j.jmii.2025.07.006_bib10","doi-asserted-by":"crossref","first-page":"816","DOI":"10.1002\/phar.2806","article-title":"Current therapies and challenges for the treatment of Staphylococcus aureus biofilm-related infections","volume":"43","author":"Tran","year":"2023","journal-title":"Pharmacotherapy"},{"key":"10.1016\/j.jmii.2025.07.006_bib11","doi-asserted-by":"crossref","DOI":"10.1016\/j.micpath.2024.107145","article-title":"Quinic acid enhances kanamycin efficacy against methicillin-resistant Staphylococcus aureus biofilms","volume":"198","author":"Bisso","year":"2025","journal-title":"Microb Pathog"},{"key":"10.1016\/j.jmii.2025.07.006_bib12","doi-asserted-by":"crossref","DOI":"10.1016\/j.bioorg.2021.104639","article-title":"Quinolines, a perpetual, multipurpose scaffold in medicinal chemistry","volume":"109","author":"Yadav","year":"2021","journal-title":"Bioorg Chem"},{"key":"10.1016\/j.jmii.2025.07.006_bib13","doi-asserted-by":"crossref","first-page":"18594","DOI":"10.1039\/D2RA02896D","article-title":"Recent advances in chemistry and therapeutic potential of functionalized quinoline motifs - a review","volume":"12","author":"Ajani","year":"2022","journal-title":"RSC Adv"},{"key":"10.1016\/j.jmii.2025.07.006_bib14","doi-asserted-by":"crossref","DOI":"10.1080\/22221751.2024.2321981","article-title":"AMXT-1501 targets membrane phospholipids against Gram-positive and -negative multidrug-resistant bacteria","volume":"13","author":"Zheng","year":"2024","journal-title":"Emerg Microb Infect"},{"key":"10.1016\/j.jmii.2025.07.006_bib15","doi-asserted-by":"crossref","first-page":"3568","DOI":"10.1093\/jac\/dkaa371","article-title":"Aspirin, sodium benzoate and sodium salicylate reverse resistance to colistin in Enterobacteriaceae and Pseudomonas aeruginosa","volume":"75","author":"Malla","year":"2020","journal-title":"J Antimicrob Chemother"},{"key":"10.1016\/j.jmii.2025.07.006_bib16","doi-asserted-by":"crossref","DOI":"10.1128\/aac.01627-23","article-title":"Sensitizing methicillin-resistant Staphylococcus aureus (MRSA) to cefuroxime: the synergic effect of bicarbonate and the wall teichoic acid inhibitor ticlopidine","volume":"68","author":"Ersoy","year":"2024","journal-title":"Antimicrob Agents Chemother"},{"key":"10.1016\/j.jmii.2025.07.006_bib17","doi-asserted-by":"crossref","DOI":"10.1016\/j.micpath.2022.105496","article-title":"Luteolin attenuates the pathogenesis of Staphylococcus aureus by interfering with the agr system","volume":"165","author":"Yuan","year":"2022","journal-title":"Microb Pathog"},{"key":"10.1016\/j.jmii.2025.07.006_bib18","doi-asserted-by":"crossref","DOI":"10.1126\/sciadv.aay9597","article-title":"A new coumarin compound DCH combats methicillin-resistant Staphylococcus aureus biofilm by targeting arginine repressor","volume":"6","author":"Qu","year":"2020","journal-title":"Sci Adv"},{"key":"10.1016\/j.jmii.2025.07.006_bib19","doi-asserted-by":"crossref","DOI":"10.1128\/aac.01001-23","article-title":"NorA efflux pump mediates Staphylococcus aureus response to Pseudomonas aeruginosa pyocyanin toxicity","volume":"68","author":"Truong-Bolduc","year":"2024","journal-title":"Antimicrob Agents Chemother"},{"key":"10.1016\/j.jmii.2025.07.006_bib20","article-title":"Diclofenac resensitizes methicillin-resistant Staphylococcus aureus to beta-lactams and prevents implant infections","volume":"8","author":"Zhang","year":"2021","journal-title":"Adv Sci (Weinh)"},{"key":"10.1016\/j.jmii.2025.07.006_bib21","doi-asserted-by":"crossref","DOI":"10.1038\/s41598-024-67347-5","article-title":"Synergistic antibacterial activity of ceftazidime-avibactam in combination with colistin, gentamicin, amikacin, and fosfomycin against carbapenem-resistant Klebsiella pneumoniae","volume":"14","author":"Tuzemen","year":"2024","journal-title":"Sci Rep"},{"key":"10.1016\/j.jmii.2025.07.006_bib22","first-page":"272","article-title":"Felodipine enhances aminoglycosides efficacy against implant infections caused by methicillin-resistant Staphylococcus aureus, persisters and biofilms","volume":"14","author":"Zhang","year":"2022","journal-title":"Bioact Mater"},{"key":"10.1016\/j.jmii.2025.07.006_bib23","doi-asserted-by":"crossref","first-page":"455","DOI":"10.1002\/jcc.21334","article-title":"AutoDock Vina: improving the speed and accuracy of docking with a new scoring function, efficient optimization, and multithreading","volume":"31","author":"Trott","year":"2010","journal-title":"J Comput Chem"},{"key":"10.1016\/j.jmii.2025.07.006_bib24","doi-asserted-by":"crossref","first-page":"433","DOI":"10.1002\/bmb.20966","article-title":"Using the PyMOL application to reinforce visual understanding of protein structure","volume":"44","author":"Rigsby","year":"2016","journal-title":"Biochem Mol Biol Educ"},{"key":"10.1016\/j.jmii.2025.07.006_bib25","doi-asserted-by":"crossref","first-page":"1701","DOI":"10.1002\/jcc.20291","article-title":"GROMACS: fast, flexible, and free","volume":"26","author":"Van Der Spoel","year":"2005","journal-title":"J Comput Chem"},{"key":"10.1016\/j.jmii.2025.07.006_bib26","doi-asserted-by":"crossref","DOI":"10.1073\/pnas.2317756121","article-title":"Discovery of lirafugratinib (RLY-4008), a highly selective irreversible small-molecule inhibitor of FGFR2","volume":"121","author":"Schonherr","year":"2024","journal-title":"Proc Natl Acad Sci U S A"},{"key":"10.1016\/j.jmii.2025.07.006_bib27","doi-asserted-by":"crossref","first-page":"501","DOI":"10.1002\/1096-987X(20010415)22:5<501::AID-JCC1021>3.0.CO;2-V","article-title":"A fast SHAKE algorithm to solve distance constraint equations for small molecules in molecular dynamics simulations","volume":"22","author":"Kr\u00e4utler","year":"2001","journal-title":"J Comput Chem"},{"key":"10.1016\/j.jmii.2025.07.006_bib28","doi-asserted-by":"crossref","first-page":"4180","DOI":"10.1021\/acs.nanolett.8b01000","article-title":"Block copolymer nanoparticles remove biofilms of drug-resistant gram-positive bacteria by nanoscale bacterial debridement","volume":"18","author":"Li","year":"2018","journal-title":"Nano Lett"},{"key":"10.1016\/j.jmii.2025.07.006_bib29","first-page":"870","article-title":"Structural basis for the beta lactam resistance of PBP2a from methicillin-resistant Staphylococcus aureus","volume":"9","author":"Lim","year":"2002","journal-title":"Nat Struct Biol"},{"key":"10.1016\/j.jmii.2025.07.006_bib30","doi-asserted-by":"crossref","first-page":"107","DOI":"10.3389\/fcimb.2020.00107","article-title":"Prevalence and therapies of antibiotic-resistance in Staphylococcus aureus","volume":"10","author":"Guo","year":"2020","journal-title":"Front Cell Infect Microbiol"},{"key":"10.1016\/j.jmii.2025.07.006_bib31","doi-asserted-by":"crossref","first-page":"689","DOI":"10.1038\/nrmicro.2017.75","article-title":"Prediction of antibiotic resistance: time for a new preclinical paradigm?","volume":"15","author":"Sommer","year":"2017","journal-title":"Nat Rev Microbiol"},{"key":"10.1016\/j.jmii.2025.07.006_bib32","doi-asserted-by":"crossref","DOI":"10.1371\/journal.ppat.1009468","article-title":"Staphylococcus aureus cell wall structure and dynamics during host-pathogen interaction","volume":"17","author":"Sutton","year":"2021","journal-title":"PLoS Pathog"},{"key":"10.1016\/j.jmii.2025.07.006_bib33","doi-asserted-by":"crossref","first-page":"9791","DOI":"10.1021\/jacs.7b04881","article-title":"Peptidoglycan cross-linking preferences of Staphylococcus aureus penicillin-binding proteins have implications for treating MRSA infections","volume":"139","author":"Srisuknimit","year":"2017","journal-title":"J Am Chem Soc"},{"key":"10.1016\/j.jmii.2025.07.006_bib34","doi-asserted-by":"crossref","first-page":"208","DOI":"10.1016\/j.ejps.2018.11.005","article-title":"Interruption in membrane permeability of drug-resistant Staphylococcus aureus with cationic particles of nano-silver","volume":"127","author":"Anuj","year":"2019","journal-title":"Eur J Pharmaceut Sci"},{"key":"10.1016\/j.jmii.2025.07.006_bib35","doi-asserted-by":"crossref","first-page":"6525","DOI":"10.1128\/JB.183.22.6525-6531.2001","article-title":"Complementation of the essential peptidoglycan transpeptidase function of penicillin-binding protein 2 (PBP2) by the drug resistance protein PBP2A in Staphylococcus aureus","volume":"183","author":"Pinho","year":"2001","journal-title":"J Bacteriol"},{"key":"10.1016\/j.jmii.2025.07.006_bib36","doi-asserted-by":"crossref","first-page":"36854","DOI":"10.1074\/jbc.M112.395962","article-title":"Properties of a novel PBP2A protein homolog from Staphylococcus aureus strain LGA251 and its contribution to the beta-lactam-resistant phenotype","volume":"287","author":"Kim","year":"2012","journal-title":"J Biol Chem"},{"key":"10.1016\/j.jmii.2025.07.006_bib37","doi-asserted-by":"crossref","first-page":"1743","DOI":"10.2217\/fmb.15.69","article-title":"Bacterial resistance in biofilm-associated bacteria","volume":"10","author":"Venkatesan","year":"2015","journal-title":"Future Microbiol"},{"key":"10.1016\/j.jmii.2025.07.006_bib38","doi-asserted-by":"crossref","first-page":"754","DOI":"10.1021\/jacs.5b11834","article-title":"Cationic pillararenes potently inhibit biofilm formation without affecting bacterial growth and viability","volume":"138","author":"Joseph","year":"2016","journal-title":"J Am Chem Soc"},{"key":"10.1016\/j.jmii.2025.07.006_bib39","doi-asserted-by":"crossref","DOI":"10.3390\/microorganisms9061124","article-title":"Biofilm-forming potential of ocular fluid Staphylococcus aureus and Staphylococcus epidermidis on ex vivo human corneas from attachment to dispersal phase","volume":"9","author":"Konduri","year":"2021","journal-title":"Microorganisms"},{"key":"10.1016\/j.jmii.2025.07.006_bib40","doi-asserted-by":"crossref","DOI":"10.1016\/j.bioflm.2023.100175","article-title":"Staphylococcus aureus biofilm: formulation, regulatory, and emerging natural products-derived therapeutics","volume":"7","author":"Wu","year":"2024","journal-title":"Biofilm"},{"key":"10.1016\/j.jmii.2025.07.006_bib41","doi-asserted-by":"crossref","DOI":"10.3390\/microorganisms12020293","article-title":"Investigation of Staphylococcus aureus biofilm-associated toxin as a potential squamous cell carcinoma therapeutic","volume":"12","author":"Ong","year":"2024","journal-title":"Microorganisms"},{"key":"10.1016\/j.jmii.2025.07.006_bib42","doi-asserted-by":"crossref","first-page":"1927","DOI":"10.1128\/IAI.00046-11","article-title":"Role of the accessory gene regulator agr in community-associated methicillin-resistant Staphylococcus aureus pathogenesis","volume":"79","author":"Cheung","year":"2011","journal-title":"Infect Immun"},{"key":"10.1016\/j.jmii.2025.07.006_bib43","article-title":"A review of biofilm formation of Staphylococcus aureus and its regulation mechanism","volume":"12","author":"Peng","year":"2022","journal-title":"Antibiotics (Basel)"},{"key":"10.1016\/j.jmii.2025.07.006_bib44","doi-asserted-by":"crossref","first-page":"1290","DOI":"10.3389\/fmicb.2017.01290","article-title":"Staphylococcus aureus quorum regulator SarA targeted compound, 2-[(Methylamino)methyl]phenol inhibits biofilm and down-regulates virulence genes","volume":"8","author":"Balamurugan","year":"2017","journal-title":"Front Microbiol"},{"key":"10.1016\/j.jmii.2025.07.006_bib45","doi-asserted-by":"crossref","first-page":"1075","DOI":"10.1046\/j.1365-2958.2003.03493.x","article-title":"SarA and not sigmaB is essential for biofilm development by Staphylococcus aureus","volume":"48","author":"Valle","year":"2003","journal-title":"Mol Microbiol"},{"key":"10.1016\/j.jmii.2025.07.006_bib46","doi-asserted-by":"crossref","first-page":"153","DOI":"10.1007\/978-1-0716-2855-3_8","article-title":"Cytotoxicity assays as predictors of the safety and efficacy of antimicrobial agents","volume":"2601","author":"Zipperer","year":"2023","journal-title":"Methods Mol Biol"}],"container-title":["Journal of Microbiology, Immunology and Infection"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1684118225001410?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1684118225001410?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,5,1]],"date-time":"2026-05-01T07:33:57Z","timestamp":1777620837000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S1684118225001410"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,2]]},"references-count":46,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2026,2]]}},"alternative-id":["S1684118225001410"],"URL":"https:\/\/doi.org\/10.1016\/j.jmii.2025.07.006","relation":{},"ISSN":["1684-1182"],"issn-type":[{"value":"1684-1182","type":"print"}],"subject":[],"published":{"date-parts":[[2026,2]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"The novel pyridine-fused quinolinone PQ-L5 exhibits antibacterial effects and enhances \u03b2-lactam activity by targeting PBP2a in methicillin-resistant Staphylococcus aureus","name":"articletitle","label":"Article Title"},{"value":"Journal of Microbiology, Immunology and Infection","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.jmii.2025.07.006","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2025 Taiwan Society of Microbiology. Published by Elsevier Taiwan LLC.","name":"copyright","label":"Copyright"}]}}