{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,26]],"date-time":"2026-08-26T08:10:46Z","timestamp":1787731846693,"version":"build-2784847793"},"reference-count":37,"publisher":"Elsevier BV","issue":"3","license":[{"start":{"date-parts":[[2015,5,1]],"date-time":"2015-05-01T00:00:00Z","timestamp":1430438400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2019,12,13]],"date-time":"2019-12-13T00:00:00Z","timestamp":1576195200000},"content-version":"vor","delay-in-days":1687,"URL":"http:\/\/www.elsevier.com\/open-access\/userlicense\/1.0\/"}],"funder":[{"DOI":"10.13039\/501100002960","name":"V\u00e4sterbotten County Council","doi-asserted-by":"crossref","award":["ALFVLL-151871"],"award-info":[{"award-number":["ALFVLL-151871"]}],"id":[{"id":"10.13039\/501100002960","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100002960","name":"V\u00e4sterbotten County Council","doi-asserted-by":"crossref","award":["ALFVLL-402201"],"award-info":[{"award-number":["ALFVLL-402201"]}],"id":[{"id":"10.13039\/501100002960","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["clinicalkey.jp","clinicalkey.com","journals.elsevierhealth.com","clinicalkey.es","clinicalkey.fr","clinicalkey.com.au","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["The Journal of Molecular Diagnostics"],"published-print":{"date-parts":[[2015,5]]},"DOI":"10.1016\/j.jmoldx.2015.01.005","type":"journal-article","created":{"date-parts":[[2015,3,13]],"date-time":"2015-03-13T01:18:28Z","timestamp":1426209508000},"page":"315-324","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":27,"title":["Development and Laboratory Evaluation of a Real-Time PCR Assay for Detecting Viruses and Bacteria of Relevance for Community-Acquired Pneumonia"],"prefix":"10.1016","volume":"17","author":[{"given":"Alicia","family":"Edin","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Susanne","family":"Granholm","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Satu","family":"Koskiniemi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Annika","family":"Allard","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anders","family":"Sj\u00f6stedt","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anders","family":"Johansson","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"issue":"Pt 3","key":"10.1016\/j.jmoldx.2015.01.005_bib1","doi-asserted-by":"crossref","first-page":"441","DOI":"10.1099\/jmm.0.067108-0","article-title":"The value of signs and symptoms in differentiating between bacterial, viral and mixed aetiology in patients with community-acquired pneumonia","volume":"63","author":"Huijskens","year":"2014","journal-title":"J Med Microbiol"},{"issue":"Suppl 3","key":"10.1016\/j.jmoldx.2015.01.005_bib2","doi-asserted-by":"crossref","first-page":"S139","DOI":"10.1093\/cid\/cit578","article-title":"Better tests, better care: improved diagnostics for infectious diseases","volume":"57","author":"Caliendo","year":"2013","journal-title":"Clin Infect Dis"},{"key":"10.1016\/j.jmoldx.2015.01.005_bib3","doi-asserted-by":"crossref","first-page":"85","DOI":"10.1016\/S0732-8893(02)00484-4","article-title":"Development of a multiplex real-time quantitative PCR assay to detect Chlamydia pneumoniae, Legionella pneumophila and Mycoplasma pneumoniae in respiratory tract secretions","volume":"45","author":"Welti","year":"2003","journal-title":"Diagn Microbiol Infect Dis"},{"key":"10.1016\/j.jmoldx.2015.01.005_bib4","doi-asserted-by":"crossref","first-page":"727","DOI":"10.1128\/JCM.01540-07","article-title":"Simultaneous detection of Chlamydophila pneumoniae and Mycoplasma pneumoniae by use of molecular beacons in a duplex real-time PCR","volume":"46","author":"Gullsby","year":"2008","journal-title":"J Clin Microbiol"},{"key":"10.1016\/j.jmoldx.2015.01.005_bib5","doi-asserted-by":"crossref","first-page":"310","DOI":"10.1186\/1471-2180-10-310","article-title":"Multiplex quantitative PCR for detection of lower respiratory tract infection and meningitis caused by Streptococcus pneumoniae, Haemophilus influenzae and Neisseria meningitidis","volume":"10","author":"Abdeldaim","year":"2010","journal-title":"BMC Microbiol"},{"key":"10.1016\/j.jmoldx.2015.01.005_bib6","doi-asserted-by":"crossref","first-page":"1386","DOI":"10.1128\/JCM.41.4.1386-1390.2003","article-title":"Quantitative detection of Moraxella catarrhalis in nasopharyngeal secretions by real-time PCR","volume":"41","author":"Greiner","year":"2003","journal-title":"J Clin Microbiol"},{"key":"10.1016\/j.jmoldx.2015.01.005_bib7","doi-asserted-by":"crossref","first-page":"3641","DOI":"10.1128\/JCM.01056-07","article-title":"Quantitative detection of Staphylococcus aureus and Enterococcus faecalis DNA in blood to diagnose bacteremia in patients in the intensive care unit","volume":"45","author":"Peters","year":"2007","journal-title":"J Clin Microbiol"},{"key":"10.1016\/j.jmoldx.2015.01.005_bib8","doi-asserted-by":"crossref","first-page":"169","DOI":"10.1016\/j.diagmicrobio.2006.01.007","article-title":"Quantitative detection of Streptococcus pneumoniae, Haemophilus influenzae, and Moraxella catarrhalis in lower respiratory tract samples by real-time PCR","volume":"55","author":"Kais","year":"2006","journal-title":"Diagn Microbiol Infect Dis"},{"key":"10.1016\/j.jmoldx.2015.01.005_bib9","doi-asserted-by":"crossref","first-page":"1653","DOI":"10.1128\/JCM.02184-10","article-title":"Simultaneous detection of influenza A, influenza B, and respiratory syncytial viruses and subtyping of influenza A H3N2 virus and H1N1 (2009) virus by multiplex real-time PCR","volume":"49","author":"Chen","year":"2011","journal-title":"J Clin Microbiol"},{"key":"10.1016\/j.jmoldx.2015.01.005_bib10","doi-asserted-by":"crossref","first-page":"484","DOI":"10.1002\/jmv.20008","article-title":"Simultaneous detection of fourteen respiratory viruses in clinical specimens by two multiplex reverse transcription nested-PCR assays","volume":"72","author":"Coiras","year":"2004","journal-title":"J Med Virol"},{"key":"10.1016\/j.jmoldx.2015.01.005_bib11","doi-asserted-by":"crossref","first-page":"254","DOI":"10.1016\/j.jviromet.2010.02.005","article-title":"A novel multiplex real-time RT-PCR assay with FRET hybridization probes for the detection and quantitation of 13 respiratory viruses","volume":"165","author":"Lassauni\u00e8re","year":"2010","journal-title":"J Virol Methods"},{"key":"10.1016\/j.jmoldx.2015.01.005_bib12","doi-asserted-by":"crossref","first-page":"53","DOI":"10.1016\/j.jcv.2007.10.029","article-title":"Multiplex real-time PCR for detection of respiratory tract infections","volume":"41","author":"Brittain-Long","year":"2008","journal-title":"J Clin Virol"},{"key":"10.1016\/j.jmoldx.2015.01.005_bib13","doi-asserted-by":"crossref","first-page":"222","DOI":"10.1016\/j.mimet.2014.10.009","article-title":"Evaluation of fast-track diagnostics and TaqMan array card real-time PCR assays for the detection of respiratory pathogens","volume":"107","author":"Driscoll","year":"2014","journal-title":"J Microbiol Methods"},{"key":"10.1016\/j.jmoldx.2015.01.005_bib14","doi-asserted-by":"crossref","first-page":"187","DOI":"10.1128\/MMBR.65.2.187-207.2001","article-title":"Pneumococcal virulence factors: structure and function","volume":"65","author":"Jedrzejas","year":"2001","journal-title":"Microbiol Mol Biol Rev"},{"key":"10.1016\/j.jmoldx.2015.01.005_bib15","doi-asserted-by":"crossref","first-page":"1080","DOI":"10.1086\/521194","article-title":"A reservoir of Moraxella catarrhalis in human pharyngeal lymphoid tissue","volume":"196","author":"Heiniger","year":"2007","journal-title":"J Infect Dis"},{"key":"10.1016\/j.jmoldx.2015.01.005_bib16","doi-asserted-by":"crossref","first-page":"564","DOI":"10.1016\/j.resmic.2005.01.010","article-title":"Rapid identification of Streptococcus pyogenes with PCR primers from a putative transcriptional regulator gene","volume":"156","author":"Liu","year":"2005","journal-title":"Res Microbiol"},{"key":"10.1016\/j.jmoldx.2015.01.005_bib17","doi-asserted-by":"crossref","first-page":"99","DOI":"10.1111\/j.1600-0463.2005.apm1130203.x","article-title":"Design of a multiplex PCR for Streptococcus pneumoniae, Haemophilus influenzae, Mycoplasma pneumoniae and Chlamydophila pneumoniae to be used on sputum samples","volume":"113","author":"Str\u00e5lin","year":"2005","journal-title":"APMIS"},{"key":"10.1016\/j.jmoldx.2015.01.005_bib18","doi-asserted-by":"crossref","first-page":"366","DOI":"10.1016\/j.diagmicrobio.2009.03.030","article-title":"Detection of Haemophilus influenzae in respiratory secretions from pneumonia patients by quantitative real-time polymerase chain reaction","volume":"64","author":"Abdeldaim","year":"2009","journal-title":"Diagn Microbiol Infect Dis"},{"key":"10.1016\/j.jmoldx.2015.01.005_bib19","doi-asserted-by":"crossref","first-page":"62","DOI":"10.1007\/s12033-011-9417-3","article-title":"Development of defined microbial population standards using fluorescence activated cell sorting for the absolute quantification of S. aureus using real-time PCR","volume":"50","author":"Martinon","year":"2012","journal-title":"Mol Biotechnol"},{"key":"10.1016\/j.jmoldx.2015.01.005_bib20","doi-asserted-by":"crossref","first-page":"3071","DOI":"10.1128\/JCM.00795-11","article-title":"Multiplex real-time PCR assay for detection of methicillin-resistant Staphylococcus aureus and associated toxin genes","volume":"49","author":"Fosheim","year":"2011","journal-title":"J Clin Microbiol"},{"key":"10.1016\/j.jmoldx.2015.01.005_bib21","doi-asserted-by":"crossref","first-page":"1564","DOI":"10.1128\/JCM.42.4.1564-1569.2004","article-title":"Rapid and sensitive method using multiplex real-time PCR for diagnosis of infections by influenza a and influenza B viruses, respiratory syncytial virus, and parainfluenza viruses 1, 2, 3, and 4","volume":"42","author":"Templeton","year":"2004","journal-title":"J Clin Microbiol"},{"key":"10.1016\/j.jmoldx.2015.01.005_bib22","doi-asserted-by":"crossref","first-page":"5189","DOI":"10.1128\/JCM.42.11.5189-5198.2004","article-title":"Real-time quantitative PCR assays for detection and monitoring of pathogenic human viruses in immunosuppressed","volume":"42","author":"Watzinger","year":"2004","journal-title":"J Clin Microbiol"},{"key":"10.1016\/j.jmoldx.2015.01.005_bib23","doi-asserted-by":"crossref","first-page":"179","DOI":"10.1016\/S1386-6532(03)00122-7","article-title":"Design and performance testing of quantitative real time PCR assays for influenza A and B viral load measurement","volume":"29","author":"Ward","year":"2004","journal-title":"J Clin Virol"},{"key":"10.1016\/j.jmoldx.2015.01.005_bib24","doi-asserted-by":"crossref","first-page":"611","DOI":"10.1373\/clinchem.2008.112797","article-title":"The MIQE guidelines: minimum information for publication of quantitative real-time PCR experiments","volume":"55","author":"Bustin","year":"2009","journal-title":"Clin Chem"},{"key":"10.1016\/j.jmoldx.2015.01.005_bib25","doi-asserted-by":"crossref","first-page":"724","DOI":"10.1136\/jcp.31.8.724","article-title":"Evaluation of some methods for the laboratory examination of sputum","volume":"31","author":"Tebbutt","year":"1978","journal-title":"J Clin Pathol"},{"key":"10.1016\/j.jmoldx.2015.01.005_bib26","doi-asserted-by":"crossref","first-page":"329","DOI":"10.1023\/A:1007385104687","article-title":"Specific detection of Coxiella burnetii through partial amplification of 23S rDNA","volume":"13","author":"Ibrahim","year":"1997","journal-title":"Eur J Epidemiol"},{"key":"10.1016\/j.jmoldx.2015.01.005_bib27","doi-asserted-by":"crossref","first-page":"134","DOI":"10.1186\/1471-2105-13-134","article-title":"Primer-BLAST: a tool to design target-specific primers for polymerase chain reaction","volume":"13","author":"Ye","year":"2012","journal-title":"BMC Bioinformatics"},{"key":"10.1016\/j.jmoldx.2015.01.005_bib28","unstructured":"Codex Committee on Methods of Analysis and Sampling. 27th Session of the European Community Comments on items 4, 7 and 9, 2006 May 15-19. Budapest, Hungary. pp 1\u20136"},{"key":"10.1016\/j.jmoldx.2015.01.005_bib29","first-page":"129","article-title":"SPUD qPCR assay confirms PREXCEL-Q softwares ability to avoid qPCR inhibition","volume":"12","author":"Gallup","year":"2010","journal-title":"Curr Issues Mol Biol"},{"key":"10.1016\/j.jmoldx.2015.01.005_bib30","doi-asserted-by":"crossref","first-page":"125","DOI":"10.1016\/j.diagmicrobio.2008.05.004","article-title":"Design and implementation of a protocol for the detection of Legionella in clinical and environmental samples","volume":"62","author":"Nazarian","year":"2008","journal-title":"Diagn Microbiol Infect Dis"},{"key":"10.1016\/j.jmoldx.2015.01.005_bib31","series-title":"Referensmetodik: Nedre luftv\u00e4gsinfektioner","year":"2005"},{"key":"10.1016\/j.jmoldx.2015.01.005_bib32","doi-asserted-by":"crossref","first-page":"609","DOI":"10.3109\/00365548.2011.570785","article-title":"Clinical impact of combined viral and bacterial infection in patients with community-acquired pneumonia","volume":"43","author":"Johansson","year":"2011","journal-title":"Scand J Infect Dis"},{"key":"10.1016\/j.jmoldx.2015.01.005_bib33","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1016\/j.jinf.2013.03.003","article-title":"Can an etiologic agent be identified in adults who are hospitalized for community-acquired pneumonia: results of a one-year study","volume":"67","author":"Musher","year":"2013","journal-title":"J Infect"},{"key":"10.1016\/j.jmoldx.2015.01.005_bib34","doi-asserted-by":"crossref","first-page":"441","DOI":"10.1097\/INF.0b013e31828ba08c","article-title":"Does respiratory virus coinfection increases the clinical severity of acute respiratory infection among children infected with respiratory syncytial virus?","volume":"32","author":"Harada","year":"2013","journal-title":"Pediatr Infect Dis J"},{"key":"10.1016\/j.jmoldx.2015.01.005_bib35","first-page":"363","article-title":"Single and multiple respiratory virus infections and severity of respiratory disease: a systematic review","volume":"15","author":"Goka","year":"2014","journal-title":"Paediatr Respir Rev"},{"key":"10.1016\/j.jmoldx.2015.01.005_bib36","doi-asserted-by":"crossref","first-page":"1000","DOI":"10.1136\/thoraxjnl-2013-203551","article-title":"Community-acquired pneumonia in Chile: the clinical relevance in the detection of viruses and atypical bacteria","volume":"68","author":"Luchsinger","year":"2013","journal-title":"Thorax"},{"key":"10.1016\/j.jmoldx.2015.01.005_bib37","doi-asserted-by":"crossref","first-page":"270","DOI":"10.1016\/j.mimet.2012.12.013","article-title":"Quantitative PCR confirms culture as the gold standard for detection of lower airway infection by nontypeable Haemophilus influenzae in Australian Indigenous children with bronchiectasis","volume":"92","author":"Hare","year":"2013","journal-title":"J Microbiol Methods"}],"container-title":["The Journal of Molecular Diagnostics"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1525157815000422?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1525157815000422?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2019,12,13]],"date-time":"2019-12-13T07:44:10Z","timestamp":1576223050000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S1525157815000422"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2015,5]]},"references-count":37,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2015,5]]}},"alternative-id":["S1525157815000422"],"URL":"https:\/\/doi.org\/10.1016\/j.jmoldx.2015.01.005","relation":{},"ISSN":["1525-1578"],"issn-type":[{"value":"1525-1578","type":"print"}],"subject":[],"published":{"date-parts":[[2015,5]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Development and Laboratory Evaluation of a Real-Time PCR Assay for Detecting Viruses and Bacteria of Relevance for Community-Acquired Pneumonia","name":"articletitle","label":"Article Title"},{"value":"The Journal of Molecular Diagnostics","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.jmoldx.2015.01.005","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"Copyright \u00a9 2015 American Society for Investigative Pathology and the Association for Molecular Pathology. Published by Elsevier Inc. All rights reserved.","name":"copyright","label":"Copyright"}]}}