{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T13:22:52Z","timestamp":1791292972109,"version":"4.1.0"},"reference-count":21,"publisher":"Elsevier BV","issue":"6","license":[{"start":{"date-parts":[[2019,11,1]],"date-time":"2019-11-01T00:00:00Z","timestamp":1572566400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2019,11,1]],"date-time":"2019-11-01T00:00:00Z","timestamp":1572566400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2020,11,1]],"date-time":"2020-11-01T00:00:00Z","timestamp":1604188800000},"content-version":"vor","delay-in-days":366,"URL":"http:\/\/www.elsevier.com\/open-access\/userlicense\/1.0\/"}],"funder":[{"name":"Vela Diagnostics"}],"content-domain":{"domain":["clinicalkey.com","clinicalkey.com.au","clinicalkey.es","clinicalkey.fr","clinicalkey.jp","jmdjournal.org","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["The Journal of Molecular Diagnostics"],"published-print":{"date-parts":[[2019,11]]},"DOI":"10.1016\/j.jmoldx.2019.06.003","type":"journal-article","created":{"date-parts":[[2019,8,2]],"date-time":"2019-08-02T19:47:58Z","timestamp":1564775278000},"page":"961-970","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":29,"title":["Prospective Evaluation of the Vela Diagnostics Next-Generation Sequencing Platform for HIV-1 Genotypic Resistance Testing"],"prefix":"10.1016","volume":"21","author":[{"given":"Jenna","family":"Weber","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ilona","family":"Volkova","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Malaya K.","family":"Sahoo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Philip L.","family":"Tzou","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robert W.","family":"Shafer","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Benjamin A.","family":"Pinsky","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.jmoldx.2019.06.003_bib1","doi-asserted-by":"crossref","first-page":"623","DOI":"10.1016\/j.jmoldx.2015.07.004","article-title":"Next-generation sequencing for infectious disease diagnosis and management: a report of the Association for Molecular Pathology","volume":"17","author":"Lefterova","year":"2015","journal-title":"J Mol Diagn"},{"key":"10.1016\/j.jmoldx.2019.06.003_bib2","doi-asserted-by":"crossref","first-page":"69","DOI":"10.1016\/j.virusres.2016.10.019","article-title":"Deep sequencing for HIV-1 clinical management","volume":"239","author":"Casadella","year":"2017","journal-title":"Virus Res"},{"key":"10.1016\/j.jmoldx.2019.06.003_bib3","doi-asserted-by":"crossref","first-page":"3380","DOI":"10.1128\/AAC.00038-16","article-title":"Low-frequency drug resistance in HIV-infected Ugandans on antiretroviral treatment is associated with regimen failure","volume":"60","author":"Kyeyune","year":"2016","journal-title":"Antimicrob Agents Chemother"},{"key":"10.1016\/j.jmoldx.2019.06.003_bib4","doi-asserted-by":"crossref","first-page":"S847","DOI":"10.1093\/infdis\/jix430","article-title":"The role of HIV-1 drug-resistant minority variants in treatment failure","volume":"216","author":"Stella-Ascariz","year":"2017","journal-title":"J Infect Dis"},{"key":"10.1016\/j.jmoldx.2019.06.003_bib5","doi-asserted-by":"crossref","first-page":"1315","DOI":"10.1097\/QAD.0000000000000267","article-title":"Comparison of ultra-deep versus Sanger sequencing detection of minority mutations on the HIV-1 drug resistance interpretations after virological failure","volume":"28","author":"Mohamed","year":"2014","journal-title":"AIDS"},{"key":"10.1016\/j.jmoldx.2019.06.003_bib6","doi-asserted-by":"crossref","first-page":"1667","DOI":"10.1093\/cid\/cit125","article-title":"Clinical implications of HIV-1 minority variants","volume":"56","author":"Li","year":"2013","journal-title":"Clin Infect Dis"},{"key":"10.1016\/j.jmoldx.2019.06.003_bib7","doi-asserted-by":"crossref","first-page":"1327","DOI":"10.1001\/jama.2011.375","article-title":"Low-frequency HIV-1 drug resistance mutations and risk of NNRTI-based antiretroviral treatment failure: a systematic review and pooled analysis","volume":"305","author":"Li","year":"2011","journal-title":"JAMA"},{"key":"10.1016\/j.jmoldx.2019.06.003_bib8","doi-asserted-by":"crossref","first-page":"e579","DOI":"10.1016\/S2352-3018(16)30119-9","article-title":"Pretreatment HIV-drug resistance in Mexico and its impact on the effectiveness of first-line antiretroviral therapy: a nationally representative 2015 WHO survey","volume":"3","author":"\u00c1vila-Rios","year":"2016","journal-title":"Lancet HIV"},{"key":"10.1016\/j.jmoldx.2019.06.003_bib9","doi-asserted-by":"crossref","first-page":"e00105-18","DOI":"10.1128\/JCM.00105-18","article-title":"Comparison of an in\u00a0vitro diagnostic next-generation sequencing assay with Sanger sequencing for HIV-1 genotypic resistance testing","volume":"56","author":"Tzou","year":"2018","journal-title":"J Clin Microbiol"},{"key":"10.1016\/j.jmoldx.2019.06.003_bib10","doi-asserted-by":"crossref","first-page":"25","DOI":"10.1373\/jalm.2016.020131","article-title":"A novel system control for quality control of diagnostic tests based on next-generation sequencing","volume":"1","author":"Chan","year":"2016","journal-title":"J Applied Lab Med"},{"key":"10.1016\/j.jmoldx.2019.06.003_bib11","doi-asserted-by":"crossref","first-page":"e0181357","DOI":"10.1371\/journal.pone.0181357","article-title":"Collaborative update of a rule-based expert system for HIV-1 genotypic resistance test interpretation","volume":"12","author":"Paredes","year":"2017","journal-title":"PLoS One"},{"key":"10.1016\/j.jmoldx.2019.06.003_bib12","doi-asserted-by":"crossref","first-page":"6058","DOI":"10.1128\/JVI.00495-16","article-title":"HIV-1 protease, reverse transcriptase, and integrase variation","volume":"90","author":"Rhee","year":"2016","journal-title":"J Virol"},{"key":"10.1016\/j.jmoldx.2019.06.003_bib13","doi-asserted-by":"crossref","first-page":"589","DOI":"10.1093\/bioinformatics\/btp698","article-title":"Fast and accurate long-read alignment with Burrows-Wheeler transform","volume":"26","author":"Li","year":"2010","journal-title":"Bioinformatics"},{"key":"10.1016\/j.jmoldx.2019.06.003_bib14","doi-asserted-by":"crossref","first-page":"1936","DOI":"10.1128\/JCM.06689-11","article-title":"Automating HIV drug resistance genotyping with RECall, a freely accessible sequence analysis tool","volume":"50","author":"Woods","year":"2012","journal-title":"J Clin Microbiol"},{"key":"10.1016\/j.jmoldx.2019.06.003_bib15","doi-asserted-by":"crossref","first-page":"1522","DOI":"10.1128\/JCM.39.4.1522-1529.2001","article-title":"High degree of interlaboratory reproducibility of human immunodeficiency virus type 1 protease and reverse transcriptase sequencing of plasma samples from heavily treated patients","volume":"39","author":"Shafer","year":"2001","journal-title":"J Clin Microbiol"},{"key":"10.1016\/j.jmoldx.2019.06.003_bib16","doi-asserted-by":"crossref","first-page":"1152","DOI":"10.1093\/jac\/dky003","article-title":"HIV-1 genotypic resistance testing using the Vela automated next-generation sequencing platform","volume":"73","author":"Raymond","year":"2018","journal-title":"J Antimicrob Chemother"},{"key":"10.1016\/j.jmoldx.2019.06.003_bib17","doi-asserted-by":"crossref","first-page":"e0209561","DOI":"10.1371\/journal.pone.0209561","article-title":"First evaluation of the Next-Generation Sequencing platform for the detection of HIV-1 drug resistance mutations in Belgium","volume":"13","author":"Dessilly","year":"2018","journal-title":"PLoS One"},{"key":"10.1016\/j.jmoldx.2019.06.003_bib18","doi-asserted-by":"crossref","first-page":"244","DOI":"10.1016\/j.annepidem.2017.02.002","article-title":"Increasing HIV-1 subtype diversity in seven states, United States, 2006-2013","volume":"27","author":"Oster","year":"2017","journal-title":"Ann Epidemiol"},{"key":"10.1016\/j.jmoldx.2019.06.003_bib19","doi-asserted-by":"crossref","first-page":"143","DOI":"10.1016\/S1473-3099(18)30647-9","article-title":"Global and regional molecular epidemiology of HIV-1, 1990-2015: a systematic review, global survey, and trend analysis","volume":"19","author":"Hemelaar","year":"2019","journal-title":"Lancet Infect Dis"},{"key":"10.1016\/j.jmoldx.2019.06.003_bib20","series-title":"Tackling HIV Drug Resistance: Trends, Guidelines, and Global Action","year":"2017"},{"key":"10.1016\/j.jmoldx.2019.06.003_bib21","doi-asserted-by":"crossref","first-page":"6824","DOI":"10.1128\/AAC.01490-15","article-title":"HIV drug resistance testing by high-multiplex \u201cwide\u201d sequencing on the MiSeq instrument","volume":"59","author":"Lapointe","year":"2015","journal-title":"Antimicrob Agents Chemother"}],"container-title":["The Journal of Molecular Diagnostics"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1525157819303460?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1525157819303460?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,10,26]],"date-time":"2025-10-26T18:14:05Z","timestamp":1761502445000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S1525157819303460"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,11]]},"references-count":21,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2019,11]]}},"alternative-id":["S1525157819303460"],"URL":"https:\/\/doi.org\/10.1016\/j.jmoldx.2019.06.003","relation":{},"ISSN":["1525-1578"],"issn-type":[{"value":"1525-1578","type":"print"}],"subject":[],"published":{"date-parts":[[2019,11]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Prospective Evaluation of the Vela Diagnostics Next-Generation Sequencing Platform for HIV-1 Genotypic Resistance Testing","name":"articletitle","label":"Article Title"},{"value":"The Journal of Molecular Diagnostics","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.jmoldx.2019.06.003","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2019 American Society for Investigative Pathology and the Association for Molecular Pathology. Published by Elsevier Inc.","name":"copyright","label":"Copyright"}]}}