{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,3]],"date-time":"2026-03-03T02:34:25Z","timestamp":1772505265637,"version":"3.50.1"},"reference-count":17,"publisher":"Elsevier BV","issue":"7","license":[{"start":{"date-parts":[[2021,7,1]],"date-time":"2021-07-01T00:00:00Z","timestamp":1625097600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2021,7,1]],"date-time":"2021-07-01T00:00:00Z","timestamp":1625097600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2022,7,1]],"date-time":"2022-07-01T00:00:00Z","timestamp":1656633600000},"content-version":"vor","delay-in-days":365,"URL":"http:\/\/www.elsevier.com\/open-access\/userlicense\/1.0\/"}],"content-domain":{"domain":["clinicalkey.com","clinicalkey.com.au","clinicalkey.es","clinicalkey.fr","clinicalkey.jp","jmdjournal.org","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["The Journal of Molecular Diagnostics"],"published-print":{"date-parts":[[2021,7]]},"DOI":"10.1016\/j.jmoldx.2021.04.004","type":"journal-article","created":{"date-parts":[[2021,5,4]],"date-time":"2021-05-04T02:28:23Z","timestamp":1620095303000},"page":"865-871","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":5,"title":["Evaluation of Two Commercial Kits on the Automated ELITe InGenius PCR Platform for Molecular Diagnosis of Toxoplasmosis"],"prefix":"10.1016","volume":"23","author":[{"given":"C\u00e9line","family":"Nourrisson","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1471-6945","authenticated-orcid":false,"given":"Maxime","family":"Moniot","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Philippe","family":"Poirier","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5623-5664","authenticated-orcid":false,"given":"Yvon","family":"Sterkers","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1016\/j.jmoldx.2021.04.004_bib1","doi-asserted-by":"crossref","first-page":"701","DOI":"10.1016\/j.ijpara.2018.03.011","article-title":"Evolution of Toxoplasma-PCR methods and practices: a French national survey and proposal for technical guidelines","volume":"48","author":"Roux","year":"2018","journal-title":"Int J Parasitol"},{"key":"10.1016\/j.jmoldx.2021.04.004_bib2","doi-asserted-by":"crossref","first-page":"29","DOI":"10.1128\/JCM.01913-14","article-title":"Multicentric comparative assessment of the bio-evolution Toxoplasma gondii detection kit with eight laboratory-developed PCR assays for molecular diagnosis of congenital toxoplasmosis","volume":"53","author":"Filisetti","year":"2015","journal-title":"J Clin Microbiol"},{"key":"10.1016\/j.jmoldx.2021.04.004_bib3","doi-asserted-by":"crossref","first-page":"1369","DOI":"10.1128\/JCM.02379-16","article-title":"Evaluation of Toxoplasma ELITe MGB real-time PCR assay for diagnosis of toxoplasmosis","volume":"55","author":"Robert-Gangneux","year":"2017","journal-title":"J Clin Microbiol"},{"key":"10.1016\/j.jmoldx.2021.04.004_bib4","doi-asserted-by":"crossref","DOI":"10.1016\/j.cmi.2018.01.014","article-title":"Evaluation of five automated and one manual method for Toxoplasma and human DNA extraction from artificially spiked amniotic fluid","volume":"24","author":"Yera","year":"2018","journal-title":"Clin Microbiol Infect"},{"key":"10.1016\/j.jmoldx.2021.04.004_bib5","doi-asserted-by":"crossref","first-page":"3952","DOI":"10.1128\/JCM.01906-14","article-title":"Characterization and multicentric validation of a common standard for Toxoplasma gondii detection using nucleic acid amplification assays","volume":"52","author":"Varlet-Marie","year":"2014","journal-title":"J Clin Microbiol"},{"key":"10.1016\/j.jmoldx.2021.04.004_bib6","doi-asserted-by":"crossref","first-page":"799","DOI":"10.1007\/BF01701522","article-title":"Classification system and case definitions of Toxoplasma gondii infection in immunocompetent pregnant women and their congenitally infected offspring.","volume":"15","author":"Lebech","year":"1996","journal-title":"Eur J Clin Microbiol Infect Dis"},{"key":"10.1016\/j.jmoldx.2021.04.004_bib7","doi-asserted-by":"crossref","first-page":"122","DOI":"10.1007\/s10096-003-0887-5","article-title":"Usefulness of Western blot in serological follow-up of newborns suspected of congenital toxoplasmosis","volume":"22","author":"Tissot Dupont","year":"2003","journal-title":"Eur J Clin Microbiol Infect Dis"},{"key":"10.1016\/j.jmoldx.2021.04.004_bib8","doi-asserted-by":"crossref","first-page":"1188","DOI":"10.1086\/317471","article-title":"Toxoplasmosis after hematopoietic stem cell transplantation","volume":"31","author":"Martino","year":"2000","journal-title":"Clin Infect Dis"},{"key":"10.1016\/j.jmoldx.2021.04.004_bib9","doi-asserted-by":"crossref","first-page":"3216","DOI":"10.1128\/JCM.02500-09","article-title":"Multicentric comparative analytical performance study for molecular detection of low amounts of Toxoplasma gondii from simulated specimens","volume":"48","author":"Sterkers","year":"2010","journal-title":"J Clin Microbiol"},{"key":"10.1016\/j.jmoldx.2021.04.004_bib10","doi-asserted-by":"crossref","first-page":"307","DOI":"10.1016\/S0140-6736(86)90837-8","article-title":"Statistical methods for assessing agreement between two methods of clinical measurement","volume":"1","author":"Bland","year":"1986","journal-title":"Lancet"},{"key":"10.1016\/j.jmoldx.2021.04.004_bib11","doi-asserted-by":"crossref","first-page":"7","DOI":"10.1186\/1471-2334-3-7","article-title":"Comparison of two DNA targets for the diagnosis of Toxoplasmosis by real-time PCR using fluorescence resonance energy transfer hybridization probes","volume":"3","author":"Reischl","year":"2003","journal-title":"BMC Infect Dis"},{"key":"10.1016\/j.jmoldx.2021.04.004_bib12","doi-asserted-by":"crossref","first-page":"69","DOI":"10.1016\/S0020-7519(99)00170-8","article-title":"Identification of a 200- to 300-fold repetitive 529 bp DNA fragment in Toxoplasma gondii, and its use for diagnostic and quantitative PCR","volume":"30","author":"Homan","year":"2000","journal-title":"Int J Parasitol"},{"key":"10.1016\/j.jmoldx.2021.04.004_bib13","doi-asserted-by":"crossref","first-page":"e00287-19","DOI":"10.1128\/JCM.00287-19","article-title":"Evaluation of the ELITe InGenius PCR platform for detection of Mycoplasma pneumoniae","volume":"57","author":"Totten","year":"2019","journal-title":"J Clin Microbiol"},{"key":"10.1016\/j.jmoldx.2021.04.004_bib14","doi-asserted-by":"crossref","first-page":"197","DOI":"10.1016\/j.diagmicrobio.2014.08.007","article-title":"Freezing and storage at -20 \u00b0C provides adequate preservation of Toxoplasma gondii DNA for retrospective molecular analysis","volume":"80","author":"Delhaes","year":"2014","journal-title":"Diagn Microbiol Infect Dis"},{"key":"10.1016\/j.jmoldx.2021.04.004_bib15","doi-asserted-by":"crossref","first-page":"85","DOI":"10.1002\/1097-0223(200102)21:2<85::AID-PD18>3.0.CO;2-1","article-title":"Prenatal diagnosis of congenital toxoplasmosis by duplex real-time PCR using fluorescence resonance energy transfer hybridization probes","volume":"21","author":"Costa","year":"2001","journal-title":"Prenat Diagn"},{"key":"10.1016\/j.jmoldx.2021.04.004_bib16","doi-asserted-by":"crossref","first-page":"104","DOI":"10.1016\/j.patbio.2009.07.013","article-title":"Development of a real-time PCR assay for quantitative diagnosis of Toxoplasma gondii after allogeneic bone marrow transplantation","volume":"58","author":"Daval","year":"2010","journal-title":"Pathol Biol (Paris)"},{"key":"10.1016\/j.jmoldx.2021.04.004_bib17","doi-asserted-by":"crossref","first-page":"e0219276","DOI":"10.1371\/journal.pone.0219276","article-title":"Inhibition of polymerase chain reaction: pathogen-specific controls are better than human gene amplification","volume":"14","author":"Roux","year":"2019","journal-title":"PLoS One"}],"container-title":["The Journal of Molecular Diagnostics"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1525157821001136?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1525157821001136?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,10,26]],"date-time":"2025-10-26T18:15:47Z","timestamp":1761502547000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S1525157821001136"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,7]]},"references-count":17,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2021,7]]}},"alternative-id":["S1525157821001136"],"URL":"https:\/\/doi.org\/10.1016\/j.jmoldx.2021.04.004","relation":{},"ISSN":["1525-1578"],"issn-type":[{"value":"1525-1578","type":"print"}],"subject":[],"published":{"date-parts":[[2021,7]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Evaluation of Two Commercial Kits on the Automated ELITe InGenius PCR Platform for Molecular Diagnosis of Toxoplasmosis","name":"articletitle","label":"Article Title"},{"value":"The Journal of Molecular Diagnostics","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.jmoldx.2021.04.004","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2021 Association for Molecular Pathology and American Society for Investigative Pathology. Published by Elsevier Inc.","name":"copyright","label":"Copyright"}]}}