{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,11]],"date-time":"2025-11-11T13:44:38Z","timestamp":1762868678178,"version":"build-2065373602"},"reference-count":39,"publisher":"Elsevier BV","issue":"1","license":[{"start":{"date-parts":[[2022,1,1]],"date-time":"2022-01-01T00:00:00Z","timestamp":1640995200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2022,1,1]],"date-time":"2022-01-01T00:00:00Z","timestamp":1640995200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2023,1,1]],"date-time":"2023-01-01T00:00:00Z","timestamp":1672531200000},"content-version":"vor","delay-in-days":365,"URL":"http:\/\/www.elsevier.com\/open-access\/userlicense\/1.0\/"}],"funder":[{"DOI":"10.13039\/100004337","name":"Roche","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100004337","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100007054","name":"Meso Scale Diagnostics","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100007054","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003030","name":"Ag\u00e8ncia de Gesti\u00f3 d&apos;Ajuts Universitaris i de Recerca","doi-asserted-by":"publisher","award":["2017SGR1085"],"award-info":[{"award-number":["2017SGR1085"]}],"id":[{"id":"10.13039\/501100003030","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100001316","name":"Abbott Laboratories","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100001316","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100004334","name":"Merck","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100004334","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100004330","name":"GlaxoSmithKline","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100004330","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["clinicalkey.com","clinicalkey.com.au","clinicalkey.es","clinicalkey.fr","clinicalkey.jp","jmdjournal.org","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["The Journal of Molecular Diagnostics"],"published-print":{"date-parts":[[2022,1]]},"DOI":"10.1016\/j.jmoldx.2021.10.008","type":"journal-article","created":{"date-parts":[[2021,11,18]],"date-time":"2021-11-18T03:21:16Z","timestamp":1637205676000},"page":"79-87","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":3,"title":["The Isothermal Amplification AmpFire Assay for Human Papillomavirus (HPV) Detection and Genotyping in Formalin-Fixed, Paraffin-Embedded Oropharyngeal Cancer Samples"],"prefix":"10.1016","volume":"24","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5392-953X","authenticated-orcid":false,"given":"Sonia","family":"Paytubi","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Miren","family":"Taberna","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marisa","family":"Mena","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6187-3732","authenticated-orcid":false,"given":"Beatriz","family":"Quir\u00f3s","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Montserrat","family":"Gom\u00e0","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Laia","family":"Alemany","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7172-3412","authenticated-orcid":false,"given":"Francesc X.","family":"Bosch","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Miguel \u00c1.","family":"Pav\u00f3n","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"year":"2007","series-title":"International Agency for Research on Cancer IARC Monographs on the Evaluation of Carcinogenic Risks to Humans VOLUME 90 Human Papillomaviruses","author":"IARC Working Group","key":"10.1016\/j.jmoldx.2021.10.008_bib1"},{"key":"10.1016\/j.jmoldx.2021.10.008_bib2","doi-asserted-by":"crossref","first-page":"e180","DOI":"10.1016\/S2214-109X(19)30488-7","article-title":"Global burden of cancer attributable to infections in 2018: a worldwide incidence analysis","volume":"8","author":"de Martel","year":"2020","journal-title":"Lancet Glob Heal"},{"key":"10.1016\/j.jmoldx.2021.10.008_bib3","doi-asserted-by":"crossref","first-page":"2386","DOI":"10.1093\/annonc\/mdx304","article-title":"Human papillomavirus-related oropharyngeal cancer","volume":"28","author":"Taberna","year":"2017","journal-title":"Ann Oncol"},{"key":"10.1016\/j.jmoldx.2021.10.008_bib4","doi-asserted-by":"crossref","first-page":"4993","DOI":"10.1158\/0008-5472.CAN-11-3934","article-title":"Viral RNA patterns and high viral load reliably define oropharynx carcinomas with active HPV16 involvement","volume":"72","author":"Holzinger","year":"2012","journal-title":"Cancer Res"},{"key":"10.1016\/j.jmoldx.2021.10.008_bib5","doi-asserted-by":"crossref","first-page":"1104","DOI":"10.1002\/hed.25591","article-title":"HPV as a marker for molecular characterization in head and neck oncology: looking for a standardization of clinical use and of detection method(s) in clinical practice","volume":"41","author":"Bussu","year":"2019","journal-title":"Head Neck"},{"key":"10.1016\/j.jmoldx.2021.10.008_bib6","doi-asserted-by":"crossref","first-page":"2465","DOI":"10.1002\/ijc.22980","article-title":"A novel algorithm for reliable detection of human papillomavirus in paraffin embedded head and neck cancer specimen","volume":"121","author":"Smeets","year":"2007","journal-title":"Int J Cancer"},{"key":"10.1016\/j.jmoldx.2021.10.008_bib7","doi-asserted-by":"crossref","first-page":"559","DOI":"10.5858\/arpa.2017-0286-CP","article-title":"Human papillomavirus testing in head and neck carcinomas guideline from the College of American Pathologists","volume":"142","author":"Lewis","year":"2018","journal-title":"Arch Pathol Lab Med"},{"key":"10.1016\/j.jmoldx.2021.10.008_bib8","doi-asserted-by":"crossref","first-page":"24","DOI":"10.1056\/NEJMoa0912217","article-title":"Human papillomavirus and survival of patients with oropharyngeal cancer","volume":"363","author":"Ang","year":"2010","journal-title":"N Engl J Med"},{"key":"10.1016\/j.jmoldx.2021.10.008_bib9","doi-asserted-by":"crossref","first-page":"414","DOI":"10.3892\/ijo.2014.2697","article-title":"Geographical and anatomical influences on human papillomavirus prevalence diversity in head and neck squamous cell carcinoma in Germany","volume":"46","author":"Quabius","year":"2015","journal-title":"Int J Oncol"},{"key":"10.1016\/j.jmoldx.2021.10.008_bib10","doi-asserted-by":"crossref","first-page":"4","DOI":"10.1186\/1750-9378-7-4","article-title":"Evaluation of a combined triple method to detect causative HPV in oral and oropharyngeal squamous cell carcinomas: P16 immunohistochemistry, consensus PCR HPV-DNA, and in situ hybridization","volume":"7","author":"Pannone","year":"2012","journal-title":"Infect Agent Cancer"},{"key":"10.1016\/j.jmoldx.2021.10.008_bib11","doi-asserted-by":"crossref","first-page":"137","DOI":"10.1016\/j.oraloncology.2018.01.010","article-title":"Double positivity for HPV-DNA\/p16ink4a is the biomarker with strongest diagnostic accuracy and prognostic value for human papillomavirus related oropharyngeal cancer patients","volume":"78","author":"Mena","year":"2018","journal-title":"Oral Oncol"},{"key":"10.1016\/j.jmoldx.2021.10.008_bib12","first-page":"1","article-title":"Protocol for the examination of specimens from patients with carcinomas of the pharynx","author":"Seethala","year":"2013","journal-title":"College of American Pathologists"},{"key":"10.1016\/j.jmoldx.2021.10.008_bib13","doi-asserted-by":"crossref","first-page":"3152","DOI":"10.1200\/JCO.18.00684","article-title":"Human papillomavirus testing in head and neck carcinomas: ASCO clinical practice guideline endorsement of the College of American Pathologists guideline","volume":"36","author":"Fakhry","year":"2018","journal-title":"J Clin Oncol"},{"key":"10.1016\/j.jmoldx.2021.10.008_bib14","doi-asserted-by":"crossref","first-page":"771","DOI":"10.1016\/j.oraloncology.2014.05.004","article-title":"Detection of human papillomavirus (HPV) in clinical samples: evolving methods and strategies for the accurate determination of HPV status of head and neck carcinomas","volume":"50","author":"Westra","year":"2014","journal-title":"Oral Oncol"},{"key":"10.1016\/j.jmoldx.2021.10.008_bib15","doi-asserted-by":"crossref","first-page":"S88","DOI":"10.1016\/j.jcv.2015.10.007","article-title":"Detection of alpha human papillomaviruses in archival formalin-fixed, paraffin-embedded (FFPE) tissue specimens","volume":"76","author":"Kocjan","year":"2016","journal-title":"J Clin Virol"},{"year":"2007","series-title":"Pathology and Genetics of Head and Neck Tumours","author":"WHO Classification of Tumours","key":"10.1016\/j.jmoldx.2021.10.008_bib16"},{"key":"10.1016\/j.jmoldx.2021.10.008_bib17","doi-asserted-by":"crossref","first-page":"46","DOI":"10.1086\/420787","article-title":"Genomic polymorphism of human papillomavirus type 52 predisposes toward persistent infection in sexually active women","volume":"190","author":"Aho","year":"2004","journal-title":"J Infect Dis"},{"key":"10.1016\/j.jmoldx.2021.10.008_bib18","doi-asserted-by":"crossref","first-page":"419","DOI":"10.1016\/j.jmoldx.2019.12.004","article-title":"An isothermal, multiplex amplification assay for detection and genotyping of human papillomaviruses in formalin-fixed, paraffin-embedded tissues","volume":"22","author":"Tang","year":"2020","journal-title":"J Mol Diagn"},{"key":"10.1016\/j.jmoldx.2021.10.008_bib19","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1038\/s41598-017-16918-w","article-title":"Meta analysis: HPV and p16 pattern determines survival in patients with HNSCC and identifies potential new biologic subtype","volume":"7","author":"Albers","year":"2017","journal-title":"Sci Rep"},{"key":"10.1016\/j.jmoldx.2021.10.008_bib20","doi-asserted-by":"crossref","first-page":"1144","DOI":"10.1016\/j.cmi.2020.03.033","article-title":"Commercially available molecular tests for human papillomaviruses: a global overview","volume":"26","author":"Poljak","year":"2020","journal-title":"Clin Microbiol Infect"},{"key":"10.1016\/j.jmoldx.2021.10.008_bib21","doi-asserted-by":"crossref","first-page":"817","DOI":"10.1016\/j.cmi.2015.04.015","article-title":"Which high-risk HPV assays fulfil criteria for use in primary cervical cancer screening?","volume":"21","author":"Arbyn","year":"2015","journal-title":"Clin Microbiol Infect"},{"key":"10.1016\/j.jmoldx.2021.10.008_bib22","article-title":"RNA extracted from paraffin-embedded human tissues is amenable to analysis by PCR amplification","volume":"11","author":"Stanta","year":"1991","journal-title":"Biotechniques"},{"key":"10.1016\/j.jmoldx.2021.10.008_bib23","doi-asserted-by":"crossref","first-page":"910","DOI":"10.1111\/apm.12727","article-title":"Testing for high-risk HPV in cervical and tonsillar paraffin-embedded tissue using a cartridge-based assay","volume":"125","author":"Virtanen","year":"2017","journal-title":"Apmis"},{"key":"10.1016\/j.jmoldx.2021.10.008_bib24","doi-asserted-by":"crossref","first-page":"55","DOI":"10.1016\/j.jcv.2016.02.007","article-title":"Formalin fixed paraffin embedded (FFPE) material is amenable to HPV detection by the Xpert\u00ae HPV assay","volume":"77","author":"Guerendiain","year":"2016","journal-title":"J Clin Virol"},{"key":"10.1016\/j.jmoldx.2021.10.008_bib25","doi-asserted-by":"crossref","first-page":"117","DOI":"10.1016\/j.oraloncology.2017.07.016","article-title":"Evaluation of the Xpert\u00ae HPV assay in the detection of human papillomavirus in formalin-fixed paraffin-embedded oropharyngeal carcinomas","volume":"72","author":"Don\u00e0","year":"2017","journal-title":"Oral Oncol"},{"key":"10.1016\/j.jmoldx.2021.10.008_bib26","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1093\/jnci\/djv403","article-title":"HPV involvement in head and neck cancers: comprehensive assessment of biomarkers in 3680 patients","volume":"108","author":"Castellsagu\u00e9","year":"2016","journal-title":"J Natl Cancer Inst"},{"key":"10.1016\/j.jmoldx.2021.10.008_bib27","doi-asserted-by":"crossref","first-page":"1330","DOI":"10.1002\/hed.24442","article-title":"Clinical characteristics and outcomes of oropharyngeal carcinoma related to high-risk non-human papillomavirus16 viral subtypes","volume":"38","author":"Varier","year":"2016","journal-title":"Head Neck"},{"key":"10.1016\/j.jmoldx.2021.10.008_bib28","first-page":"53","article-title":"Specificity of the linear array HPV genotyping test for detecting human papillomavirus genotype 52 (HPV-52)","volume":"23","author":"O\u0161trbenk","year":"2014","journal-title":"Acta Dermatovenerol Alp Pannonica Adriat"},{"key":"10.1016\/j.jmoldx.2021.10.008_bib29","doi-asserted-by":"crossref","first-page":"113840","DOI":"10.1016\/j.jviromet.2020.113840","article-title":"Performance of AmpFire HPV assay on neck cervical lymph node aspirate and oropharyngeal samples","volume":"279","author":"Jang","year":"2020","journal-title":"J Virol Methods"},{"key":"10.1016\/j.jmoldx.2021.10.008_bib30","doi-asserted-by":"crossref","first-page":"1186","DOI":"10.1002\/ijc.30516","article-title":"Diagnostic accuracy of p16INK4a immunohistochemistry in oropharyngeal squamous cell carcinomas: a systematic review and meta-analysis","volume":"140","author":"Prigge","year":"2017","journal-title":"Int J Cancer"},{"key":"10.1016\/j.jmoldx.2021.10.008_bib31","doi-asserted-by":"crossref","first-page":"169","DOI":"10.1001\/jamaoncol.2016.3067","article-title":"Differences in the prevalence of human papillomavirus (HPV) in head and neck squamous cell cancers by sex, race, anatomic tumor site, and HPV detection method","volume":"3","author":"D\u2019Souza","year":"2017","journal-title":"JAMA Oncol"},{"key":"10.1016\/j.jmoldx.2021.10.008_bib32","first-page":"1123","article-title":"P16 and HPV discordance in oropharyngeal squamous cell carcinoma: what are the clinical implications?","volume":"3","author":"Osborn","year":"2016","journal-title":"Ann Hematol Oncol"},{"key":"10.1016\/j.jmoldx.2021.10.008_bib33","doi-asserted-by":"crossref","first-page":"2366","DOI":"10.1002\/ijc.28580","article-title":"Molecular characterization of p16-immunopositive but HPV DNA-negative oropharyngeal carcinomas","volume":"134","author":"Rietbergen","year":"2014","journal-title":"Int J Cancer"},{"key":"10.1016\/j.jmoldx.2021.10.008_bib34","doi-asserted-by":"crossref","first-page":"4142","DOI":"10.1200\/JCO.2010.29.2904","article-title":"Prognostic significance of p16INK4A and human papillomavirus in patients with oropharyngeal cancer treated on TROG 02.02 phase III trial","volume":"28","author":"Rischin","year":"2010","journal-title":"J Clin Oncol"},{"key":"10.1016\/j.jmoldx.2021.10.008_bib35","doi-asserted-by":"crossref","first-page":"620","DOI":"10.5858\/arpa.2019-0199-OA","article-title":"Evaluation of the Anyplex II HPV28 assay in the detection of human papillomavirus in archival samples of oropharyngeal carcinomas","volume":"144","author":"Rollo","year":"2019","journal-title":"Arch Pathol Lab Med"},{"key":"10.1016\/j.jmoldx.2021.10.008_bib36","doi-asserted-by":"crossref","first-page":"1813","DOI":"10.5858\/2009-0592-OAR.1","article-title":"Use of a linear array for the detection of human papillomavirus genotypes in head and neck cancer","volume":"134","author":"Black","year":"2010","journal-title":"Arch Pathol Lab Med"},{"key":"10.1016\/j.jmoldx.2021.10.008_bib37","doi-asserted-by":"crossref","first-page":"862","DOI":"10.1016\/j.oraloncology.2015.06.014","article-title":"Correlation of p16 expression and HPV type with survival in oropharyngeal squamous cell cancer","volume":"51","author":"Liu","year":"2015","journal-title":"Oral Oncol"},{"key":"10.1016\/j.jmoldx.2021.10.008_bib38","doi-asserted-by":"crossref","first-page":"297","DOI":"10.1111\/j.1750-3639.2007.00073.x","article-title":"Effects of formalin fixation, paraffin embedding, and time of storage on DNA preservation in brain tissue: a brainnet Europe study","volume":"17","author":"Ferrer","year":"2007","journal-title":"Brain Pathol"},{"key":"10.1016\/j.jmoldx.2021.10.008_bib39","doi-asserted-by":"crossref","first-page":"321","DOI":"10.1007\/s12105-019-01117-y","article-title":"Human papillomavirus testing in head and neck squamous cell carcinoma in 2020: where are we now and where are we going?","volume":"14","author":"Lewis","year":"2020","journal-title":"Head Neck Pathol"}],"container-title":["The Journal of Molecular Diagnostics"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1525157821003846?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1525157821003846?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,10,26]],"date-time":"2025-10-26T18:16:13Z","timestamp":1761502573000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S1525157821003846"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,1]]},"references-count":39,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2022,1]]}},"alternative-id":["S1525157821003846"],"URL":"https:\/\/doi.org\/10.1016\/j.jmoldx.2021.10.008","relation":{},"ISSN":["1525-1578"],"issn-type":[{"type":"print","value":"1525-1578"}],"subject":[],"published":{"date-parts":[[2022,1]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"The Isothermal Amplification AmpFire Assay for Human Papillomavirus (HPV) Detection and Genotyping in Formalin-Fixed, Paraffin-Embedded Oropharyngeal Cancer Samples","name":"articletitle","label":"Article Title"},{"value":"The Journal of Molecular Diagnostics","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.jmoldx.2021.10.008","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2022 Association for Molecular Pathology and American Society for Investigative Pathology. Published by Elsevier Inc.","name":"copyright","label":"Copyright"}]}}