{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,19]],"date-time":"2026-06-19T11:25:39Z","timestamp":1781868339296,"version":"3.54.5"},"reference-count":23,"publisher":"Elsevier BV","issue":"6","license":[{"start":{"date-parts":[[2023,6,1]],"date-time":"2023-06-01T00:00:00Z","timestamp":1685577600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2023,6,1]],"date-time":"2023-06-01T00:00:00Z","timestamp":1685577600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2024,6,1]],"date-time":"2024-06-01T00:00:00Z","timestamp":1717200000000},"content-version":"vor","delay-in-days":366,"URL":"http:\/\/www.elsevier.com\/open-access\/userlicense\/1.0\/"}],"funder":[{"DOI":"10.13039\/501100001700","name":"Ministry of Education, Culture, Sports, Science and Technology","doi-asserted-by":"publisher","award":["21K07748"],"award-info":[{"award-number":["21K07748"]}],"id":[{"id":"10.13039\/501100001700","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["clinicalkey.com","clinicalkey.com.au","clinicalkey.es","clinicalkey.fr","clinicalkey.jp","jmdjournal.org","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["The Journal of Molecular Diagnostics"],"published-print":{"date-parts":[[2023,6]]},"DOI":"10.1016\/j.jmoldx.2023.03.004","type":"journal-article","created":{"date-parts":[[2023,3,23]],"date-time":"2023-03-23T18:48:29Z","timestamp":1679597309000},"page":"403-409","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":7,"title":["Droplet Digital PCR Development for Adenovirus Load Monitoring in Children after Hematopoietic Stem Cell Transplantation"],"prefix":"10.1016","volume":"25","author":[{"given":"Kazunori","family":"Haruta","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Suguru","family":"Takeuchi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Makoto","family":"Yamaguchi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3017-299X","authenticated-orcid":false,"given":"Kazuhiro","family":"Horiba","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Takako","family":"Suzuki","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuka","family":"Torii","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Atsushi","family":"Narita","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8801-0021","authenticated-orcid":false,"given":"Hideki","family":"Muramatsu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yoshiyuki","family":"Takahashi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7199-7088","authenticated-orcid":false,"given":"Yoshinori","family":"Ito","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1553-2539","authenticated-orcid":false,"given":"Jun-ichi","family":"Kawada","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.jmoldx.2023.03.004_bib1","doi-asserted-by":"crossref","first-page":"51","DOI":"10.1038\/sj.bmt.1703083","article-title":"Adenoviral infection after allogeneic stem cell transplantation (SCT): report on 130 patients from a single SCT unit involved in a prospective multi center surveillance study","volume":"28","author":"Runde","year":"2001","journal-title":"Bone Marrow Transplant"},{"key":"10.1016\/j.jmoldx.2023.03.004_bib2","doi-asserted-by":"crossref","first-page":"555","DOI":"10.1111\/tid.12022","article-title":"Fourth European Conference on Infections in Leukemia: European guidelines for diagnosis and treatment of adenovirus infection in leukemia and stem cell transplantation: summary of ECIL-4 (2011)","volume":"14","author":"Matthes-Martin","year":"2012","journal-title":"Transpl Infect Dis"},{"key":"10.1016\/j.jmoldx.2023.03.004_bib3","doi-asserted-by":"crossref","first-page":"1114","DOI":"10.1182\/blood-2002-07-2152","article-title":"Molecular monitoring of adenovirus in peripheral blood after allogeneic bone marrow transplantation permits early diagnosis of disseminated disease","volume":"102","author":"Lion","year":"2003","journal-title":"Blood"},{"key":"10.1016\/j.jmoldx.2023.03.004_bib4","doi-asserted-by":"crossref","first-page":"267","DOI":"10.1038\/bmt.2010.86","article-title":"Evaluation of a surveillance strategy for early detection of adenovirus by PCR of peripheral blood in hematopoietic SCT recipients: incidence and outcome","volume":"46","author":"\u00d6hrmalm","year":"2011","journal-title":"Bone Marrow Transplant"},{"key":"10.1016\/j.jmoldx.2023.03.004_bib5","doi-asserted-by":"crossref","first-page":"803","DOI":"10.1038\/bmt.2012.221","article-title":"Impact of viral reactivations in the era of pre-emptive antiviral drug therapy following allogeneic haematopoietic SCT in paediatric recipients","volume":"48","author":"Hiwarkar","year":"2013","journal-title":"Bone Marrow Transplant"},{"key":"10.1016\/j.jmoldx.2023.03.004_bib6","doi-asserted-by":"crossref","first-page":"250","DOI":"10.1016\/j.bbmt.2013.11.009","article-title":"Patient, virus, and treatment-related risk factors in pediatric adenovirus infection after stem cell transplantation: results of a routine monitoring program","volume":"20","author":"Mynarek","year":"2014","journal-title":"Biol Blood Marrow Transplant"},{"key":"10.1016\/j.jmoldx.2023.03.004_bib7","doi-asserted-by":"crossref","first-page":"317","DOI":"10.1093\/jpids\/piy049","article-title":"Outcomes of human adenovirus infection and disease in a retrospective cohort of pediatric hematopoietic cell transplant recipients","volume":"8","author":"Fisher","year":"2019","journal-title":"J Pediatr Infect Dis Soc"},{"key":"10.1016\/j.jmoldx.2023.03.004_bib8","doi-asserted-by":"crossref","first-page":"810","DOI":"10.1016\/j.bbmt.2018.12.753","article-title":"Incidence of adenovirus infection in hematopoietic stem cell transplantation recipients: findings from the AdVance Study","volume":"25","author":"Sedl\u00e1\u010dek","year":"2019","journal-title":"Biol Blood Marrow Transplant"},{"key":"10.1016\/j.jmoldx.2023.03.004_bib9","doi-asserted-by":"crossref","first-page":"e14209","DOI":"10.1111\/ctr.14209","article-title":"Follow-up of human adenovirus viral load in pediatric hematopoietic stem cell transplant recipients","volume":"35","author":"Peker","year":"2021","journal-title":"Clin Transplant"},{"key":"10.1016\/j.jmoldx.2023.03.004_bib10","doi-asserted-by":"crossref","first-page":"526","DOI":"10.1086\/341770","article-title":"High levels of adenovirus DNA in serum correlate with fatal outcome of adenovirus infection in children after allogeneic stem-cell transplantation","volume":"35","author":"Schilham","year":"2002","journal-title":"Clin Infect Dis"},{"key":"10.1016\/j.jmoldx.2023.03.004_bib11","doi-asserted-by":"crossref","first-page":"414","DOI":"10.3389\/fmicb.2019.00414","article-title":"Diagnostic parameters of adenoviremia in pediatric stem cell transplant recipients","volume":"10","author":"Kosulin","year":"2019","journal-title":"Front Microbiol"},{"key":"10.1016\/j.jmoldx.2023.03.004_bib12","doi-asserted-by":"crossref","first-page":"1333","DOI":"10.1038\/sj.bmt.1702716","article-title":"Outcome and clinical course of 100 patients with adenovirus infection following bone marrow transplantation","volume":"26","author":"Baldwin","year":"2000","journal-title":"Bone Marrow Transplant"},{"key":"10.1016\/j.jmoldx.2023.03.004_bib13","doi-asserted-by":"crossref","first-page":"1619","DOI":"10.1182\/blood-2002-02-0377","article-title":"Adenovirus infections following allogeneic stem cell transplantation: incidence and outcome in relation to graft manipulation, immunosuppression, and immune recovery","volume":"100","author":"Chakrabarti","year":"2002","journal-title":"Blood"},{"key":"10.1016\/j.jmoldx.2023.03.004_bib14","doi-asserted-by":"crossref","first-page":"e1980","DOI":"10.1002\/rmv.1980","volume":"28","author":"Hiwarkar","year":"2018","journal-title":"Rev Med Virol"},{"key":"10.1016\/j.jmoldx.2023.03.004_bib15","doi-asserted-by":"crossref","first-page":"e82","DOI":"10.1093\/nar\/gks203","article-title":"Comparison of microfluidic digital PCR and conventional quantitative PCR for measuring copy number variation","volume":"40","author":"Whale","year":"2012","journal-title":"Nucleic Acids Res"},{"key":"10.1016\/j.jmoldx.2023.03.004_bib16","doi-asserted-by":"crossref","first-page":"1868","DOI":"10.1002\/jmv.25276","article-title":"Droplet-digital polymerase chain reaction for detection of clinical hepatitis B virus DNA samples","volume":"90","author":"Yang","year":"2018","journal-title":"J Med Virol"},{"key":"10.1016\/j.jmoldx.2023.03.004_bib17","doi-asserted-by":"crossref","first-page":"1305","DOI":"10.3390\/diagnostics12061305","article-title":"Digital droplet PCR in hematologic malignancies: a new useful molecular tool","volume":"12","author":"Galimberti","year":"2022","journal-title":"Diagnostics (Basel)"},{"key":"10.1016\/j.jmoldx.2023.03.004_bib18","doi-asserted-by":"crossref","first-page":"3097","DOI":"10.1111\/cas.15398","article-title":"KRAS variant allele frequency, but not mutation positivity, associates with survival of patients with pancreatic cancer","volume":"113","author":"Suzuki","year":"2022","journal-title":"Cancer Sci"},{"key":"10.1016\/j.jmoldx.2023.03.004_bib19","doi-asserted-by":"crossref","first-page":"4559","DOI":"10.1002\/jmv.27844","article-title":"Quantitative assessment of viral load in the blood and urine of patients with congenital cytomegalovirus infection using droplet digital PCR","volume":"94","author":"Yamaguchi","year":"2022","journal-title":"J Med Virol"},{"key":"10.1016\/j.jmoldx.2023.03.004_bib20","doi-asserted-by":"crossref","first-page":"228","DOI":"10.1002\/jmv.10382","article-title":"Rapid and quantitative detection of human adenovirus DNA by real-time PCR","volume":"70","author":"Heim","year":"2003","journal-title":"J Med Virol"},{"key":"10.1016\/j.jmoldx.2023.03.004_bib21","doi-asserted-by":"crossref","first-page":"1426","DOI":"10.1128\/JCM.01515-06","article-title":"Simultaneous quantification of Epstein-Barr virus, cytomegalovirus, and human herpesvirus 6 DNA in samples from transplant recipients by multiplex real-time PCR assay","volume":"45","author":"Wada","year":"2007","journal-title":"J Clin Microbiol"},{"key":"10.1016\/j.jmoldx.2023.03.004_bib22","doi-asserted-by":"crossref","first-page":"872.e1","DOI":"10.1016\/j.jtct.2021.07.014","article-title":"A prospective viral monitoring study after pediatric allogeneic hematopoietic stem cell transplantation for malignant and nonmalignant diseases","volume":"27","author":"Yamada","year":"2021","journal-title":"Transplant Cell Ther"},{"key":"10.1016\/j.jmoldx.2023.03.004_bib23","doi-asserted-by":"crossref","first-page":"441","DOI":"10.1128\/CMR.00116-13","article-title":"Adenovirus infections in immunocompetent and immunocompromised patients","volume":"27","author":"Lion","year":"2014","journal-title":"Clin Microbiol Rev"}],"container-title":["The Journal of Molecular Diagnostics"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1525157823000570?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1525157823000570?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,10,26]],"date-time":"2025-10-26T18:17:55Z","timestamp":1761502675000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S1525157823000570"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,6]]},"references-count":23,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2023,6]]}},"alternative-id":["S1525157823000570"],"URL":"https:\/\/doi.org\/10.1016\/j.jmoldx.2023.03.004","relation":{},"ISSN":["1525-1578"],"issn-type":[{"value":"1525-1578","type":"print"}],"subject":[],"published":{"date-parts":[[2023,6]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Droplet Digital PCR Development for Adenovirus Load Monitoring in Children after Hematopoietic Stem Cell Transplantation","name":"articletitle","label":"Article Title"},{"value":"The Journal of Molecular Diagnostics","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.jmoldx.2023.03.004","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2023 Association for Molecular Pathology and American Society for Investigative Pathology. Published by Elsevier Inc.","name":"copyright","label":"Copyright"}]}}