{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,3]],"date-time":"2026-11-03T06:08:41Z","timestamp":1793686121885,"version":"4.5.1"},"reference-count":32,"publisher":"Elsevier BV","issue":"7","license":[{"start":{"date-parts":[[2023,7,1]],"date-time":"2023-07-01T00:00:00Z","timestamp":1688169600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2023,7,1]],"date-time":"2023-07-01T00:00:00Z","timestamp":1688169600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2023,4,11]],"date-time":"2023-04-11T00:00:00Z","timestamp":1681171200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100019125","name":"Sorbonne Universit\u00e9","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100019125","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002738","name":"APHP","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100002738","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["clinicalkey.com","clinicalkey.com.au","clinicalkey.es","clinicalkey.fr","clinicalkey.jp","jmdjournal.org","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["The Journal of Molecular Diagnostics"],"published-print":{"date-parts":[[2023,7]]},"DOI":"10.1016\/j.jmoldx.2023.03.006","type":"journal-article","created":{"date-parts":[[2023,4,15]],"date-time":"2023-04-15T01:51:20Z","timestamp":1681523480000},"page":"467-476","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":11,"title":["Assessment of Droplet Digital PCR for the Detection and Absolute Quantification of Toxoplasma gondii"],"prefix":"10.1016","volume":"25","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7866-6468","authenticated-orcid":false,"given":"C\u00e9cile","family":"Nabet","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jean-Yves","family":"Brossas","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Corentin","family":"Poignon","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amira","family":"Bouzidi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3433-9603","authenticated-orcid":false,"given":"Luc","family":"Paris","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Feriel","family":"Touafek","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emmanuelle","family":"Varlet-Marie","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5623-5664","authenticated-orcid":false,"given":"Yvon","family":"Sterkers","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6752-8916","authenticated-orcid":false,"given":"Karine","family":"Passebosc-Faure","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9808-4569","authenticated-orcid":false,"given":"Marie-Laure","family":"Dard\u00e9","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Renaud","family":"Piarroux","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1617-4617","authenticated-orcid":false,"given":"J\u00e9r\u00f4me Alexandre","family":"Denis","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.jmoldx.2023.03.006_bib1","doi-asserted-by":"crossref","first-page":"1965","DOI":"10.1016\/S0140-6736(04)16412-X","article-title":"Toxoplasmosis","volume":"363","author":"Montoya","year":"2004","journal-title":"Lancet"},{"key":"10.1016\/j.jmoldx.2023.03.006_bib2","doi-asserted-by":"crossref","first-page":"25","DOI":"10.3390\/pathogens7010025","article-title":"Congenital toxoplasmosis: a plea for a neglected disease","volume":"7","author":"Wallon","year":"2018","journal-title":"Pathogens"},{"key":"10.1016\/j.jmoldx.2023.03.006_bib3","doi-asserted-by":"crossref","first-page":"S205","DOI":"10.1016\/S0035-9203(02)90078-7","article-title":"Diagnosis molecular diagnosis of toxoplasmosis","volume":"96","author":"Bastien","year":"2002","journal-title":"Trans R Soc Trop Med Hyg"},{"key":"10.1016\/j.jmoldx.2023.03.006_bib4","doi-asserted-by":"crossref","first-page":"797","DOI":"10.1016\/j.ajog.2003.09.039","article-title":"Usefulness of quantitative polymerase chain reaction in amniotic fluid as early prognostic marker of fetal infection with Toxoplasma gondii","volume":"190","author":"Romand","year":"2004","journal-title":"Am J Obstet Gynecol"},{"key":"10.1016\/j.jmoldx.2023.03.006_bib5","doi-asserted-by":"crossref","first-page":"85","DOI":"10.1002\/1097-0223(200102)21:2<85::AID-PD18>3.0.CO;2-1","article-title":"Prenatal diagnosis of congenital toxoplasmosis by duplex real-time PCR using fluorescence resonance energy transfer hybridization probes","volume":"21","author":"Costa","year":"2001","journal-title":"Prenat Diagn"},{"key":"10.1016\/j.jmoldx.2023.03.006_bib6","doi-asserted-by":"crossref","first-page":"2541","DOI":"10.1128\/JCM.00252-10","article-title":"Correlation of parasite load determined by quantitative PCR to clinical outcome in a heart transplant patient with disseminated toxoplasmosis","volume":"48","author":"Patrat-Delon","year":"2010","journal-title":"J Clin Microbiol"},{"key":"10.1016\/j.jmoldx.2023.03.006_bib7","doi-asserted-by":"crossref","first-page":"120","DOI":"10.1046\/j.1469-0691.2001.00224.x","article-title":"Quantitative detection of Toxoplasma gondii DNA in human body fluids by TaqMan polymerase chain reaction","volume":"7","author":"Kupferschmidt","year":"2001","journal-title":"Clin Microbiol Infect"},{"key":"10.1016\/j.jmoldx.2023.03.006_bib8","doi-asserted-by":"crossref","first-page":"3952","DOI":"10.1128\/JCM.01906-14","article-title":"Characterization and multicentric validation of a common standard for Toxoplasma gondii detection using nucleic acid amplification assays","volume":"52","author":"Varlet-Marie","year":"2014","journal-title":"J Clin Microbiol"},{"key":"10.1016\/j.jmoldx.2023.03.006_bib9","doi-asserted-by":"crossref","first-page":"1294","DOI":"10.1128\/JCM.02900-14","article-title":"A 10-year retrospective comparison of two target sequences, REP-529 and B1, for Toxoplasma gondii detection by quantitative PCR","volume":"53","author":"Belaz","year":"2015","journal-title":"J Clin Microbiol"},{"key":"10.1016\/j.jmoldx.2023.03.006_bib10","doi-asserted-by":"crossref","first-page":"7","DOI":"10.1186\/1471-2334-3-7","article-title":"Comparison of two DNA targets for the diagnosis of toxoplasmosis by real-time PCR using fluorescence resonance energy transfer hybridization probes","volume":"3","author":"Reischl","year":"2003","journal-title":"BMC Infect Dis"},{"key":"10.1016\/j.jmoldx.2023.03.006_bib11","doi-asserted-by":"crossref","first-page":"701","DOI":"10.1016\/j.ijpara.2018.03.011","article-title":"Evolution of Toxoplasma-PCR methods and practices: a French national survey and proposal for technical guidelines","volume":"48","author":"Roux","year":"2018","journal-title":"Int J Parasitol"},{"key":"10.1016\/j.jmoldx.2023.03.006_bib12","doi-asserted-by":"crossref","first-page":"69","DOI":"10.1016\/S0020-7519(99)00170-8","article-title":"Identification of a 200- to 300-fold repetitive 529 bp DNA fragment in Toxoplasma gondii, and its use for diagnostic and quantitative PCR","volume":"30","author":"Homan","year":"2000","journal-title":"Int J Parasitol"},{"key":"10.1016\/j.jmoldx.2023.03.006_bib13","doi-asserted-by":"crossref","first-page":"1100.e7","DOI":"10.1016\/j.cmi.2018.01.014","article-title":"Evaluation of five automated and one manual method for Toxoplasma and human DNA extraction from artificially spiked amniotic fluid","volume":"24","author":"Yera","year":"2018","journal-title":"Clin Microbiol Infect"},{"key":"10.1016\/j.jmoldx.2023.03.006_bib14","doi-asserted-by":"crossref","first-page":"459","DOI":"10.1016\/j.bios.2016.09.082","article-title":"Advances in digital polymerase chain reaction (dPCR) and its emerging biomedical applications","volume":"90","author":"Cao","year":"2017","journal-title":"Biosens Bioelectron"},{"key":"10.1016\/j.jmoldx.2023.03.006_bib15","doi-asserted-by":"crossref","first-page":"587","DOI":"10.1007\/s40291-017-0287-7","article-title":"The Role of BEAMing and digital PCR for multiplexed analysis in molecular oncology in the era of next-generation sequencing","volume":"21","author":"Denis","year":"2017","journal-title":"Mol Diagn Ther"},{"key":"10.1016\/j.jmoldx.2023.03.006_bib16","doi-asserted-by":"crossref","first-page":"948","DOI":"10.1016\/j.clinbiochem.2015.03.015","article-title":"Digital PCR analysis of circulating nucleic acids","volume":"48","author":"Hudecova","year":"2015","journal-title":"Clin Biochem"},{"key":"10.1016\/j.jmoldx.2023.03.006_bib17","doi-asserted-by":"crossref","first-page":"1621","DOI":"10.1128\/JCM.00211-17","article-title":"Applications of digital PCR for clinical microbiology","volume":"55","author":"Kuypers","year":"2017","journal-title":"J Clin Microbiol"},{"key":"10.1016\/j.jmoldx.2023.03.006_bib18","doi-asserted-by":"crossref","DOI":"10.1042\/BSR20181170","article-title":"Application of droplet digital PCR to detect the pathogens of infectious diseases","volume":"38","author":"Li","year":"2018","journal-title":"Biosci Rep"},{"key":"10.1016\/j.jmoldx.2023.03.006_bib19","doi-asserted-by":"crossref","first-page":"750","DOI":"10.1016\/j.ijbiomac.2021.06.132","article-title":"Digital PCR for accurate quantification of pathogens: principles, applications, challenges and future prospects","volume":"184","author":"Lei","year":"2021","journal-title":"Int J Biol Macromol"},{"key":"10.1016\/j.jmoldx.2023.03.006_bib20","doi-asserted-by":"crossref","first-page":"1510","DOI":"10.1016\/j.cmi.2019.06.009","article-title":"Digital PCR: a new technology for diagnosis of parasitic infections","volume":"25","author":"Pomari","year":"2019","journal-title":"Clin Microbiol Infect"},{"key":"10.1016\/j.jmoldx.2023.03.006_bib21","doi-asserted-by":"crossref","first-page":"3987","DOI":"10.1128\/JCM.01793-14","article-title":"PCR-based detection of Toxoplasma gondii DNA in blood and ocular samples for diagnosis of ocular toxoplasmosis","volume":"52","author":"Bourdin","year":"2014","journal-title":"J Clin Microbiol"},{"key":"10.1016\/j.jmoldx.2023.03.006_bib22","doi-asserted-by":"crossref","first-page":"1012","DOI":"10.1093\/clinchem\/hvaa125","article-title":"The digital MIQE guidelines update: minimum information for publication of quantitative digital PCR experiments for 2020","volume":"66","author":"Whale","year":"2020","journal-title":"Clin Chem"},{"key":"10.1016\/j.jmoldx.2023.03.006_bib23","doi-asserted-by":"crossref","first-page":"261","DOI":"10.1016\/0166-6851(88)90081-3","article-title":"The alpha- and beta-tubulins of Toxoplasma gondii are encoded by single copy genes containing multiple introns","volume":"29","author":"Nagel","year":"1988","journal-title":"Mol Biochem Parasitol"},{"key":"10.1016\/j.jmoldx.2023.03.006_bib24","doi-asserted-by":"crossref","first-page":"4641","DOI":"10.1128\/JCM.01152-10","article-title":"Genotyping of Toxoplasma gondii isolates with 15 microsatellite markers in a single multiplex PCR assay","volume":"48","author":"Ajzenberg","year":"2010","journal-title":"J Clin Microbiol"},{"key":"10.1016\/j.jmoldx.2023.03.006_bib25","doi-asserted-by":"crossref","first-page":"307","DOI":"10.1016\/S0140-6736(86)90837-8","article-title":"Statistical methods for assessing agreement between two methods of clinical measurement","volume":"327","author":"Martin Bland","year":"1986","journal-title":"Lancet"},{"key":"10.1016\/j.jmoldx.2023.03.006_bib26","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0175771","article-title":"Four human Plasmodium species quantification using droplet digital PCR","volume":"12","author":"Srisutham","year":"2017","journal-title":"PLoS One"},{"key":"10.1016\/j.jmoldx.2023.03.006_bib27","doi-asserted-by":"crossref","first-page":"39183","DOI":"10.1038\/srep39183","article-title":"Sensitive and accurate quantification of human malaria parasites using droplet digital PCR (ddPCR)","volume":"6","author":"Koepfli","year":"2016","journal-title":"Sci Rep"},{"key":"10.1016\/j.jmoldx.2023.03.006_bib28","doi-asserted-by":"crossref","first-page":"249","DOI":"10.1186\/s12936-018-2382-6","article-title":"Assessing Plasmodium falciparum transmission in mosquito-feeding assays using quantitative PCR","volume":"17","author":"Wang","year":"2018","journal-title":"Malar J"},{"key":"10.1016\/j.jmoldx.2023.03.006_bib32","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pntd.0007063","article-title":"Evaluation of the analytical and diagnostic performance of a digital droplet polymerase chain reaction (ddPCR) assay to detect Trypanosoma cruzi DNA in blood samples","volume":"12","author":"Ram\u00edrez","year":"2018","journal-title":"PLoS Negl Trop Dis"},{"key":"10.1016\/j.jmoldx.2023.03.006_bib29","doi-asserted-by":"crossref","first-page":"160","DOI":"10.1016\/j.mimet.2016.06.015","article-title":"Variation in copy number of the 28S rDNA of Aspergillus fumigatus measured by droplet digital PCR and analog quantitative real-time PCR","volume":"127","author":"Alanio","year":"2016","journal-title":"J Microbiol Methods"},{"key":"10.1016\/j.jmoldx.2023.03.006_bib30","doi-asserted-by":"crossref","DOI":"10.1128\/JCM.01223-19","article-title":"Is real-time PCR targeting rep 529 suitable for diagnosis of toxoplasmosis in patients infected with non-type II strains in North America?","volume":"58","author":"Pomares","year":"2020","journal-title":"J Clin Microbiol"},{"key":"10.1016\/j.jmoldx.2023.03.006_bib31","doi-asserted-by":"crossref","first-page":"1792","DOI":"10.1093\/nar\/gkh340","article-title":"MUSCLE: multiple sequence alignment with high accuracy and high throughput","volume":"32","author":"Edgar","year":"2004","journal-title":"Nucleic Acids Res"}],"container-title":["The Journal of Molecular Diagnostics"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1525157823000739?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1525157823000739?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,10,26]],"date-time":"2025-10-26T18:18:22Z","timestamp":1761502702000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S1525157823000739"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,7]]},"references-count":32,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2023,7]]}},"alternative-id":["S1525157823000739"],"URL":"https:\/\/doi.org\/10.1016\/j.jmoldx.2023.03.006","relation":{},"ISSN":["1525-1578"],"issn-type":[{"value":"1525-1578","type":"print"}],"subject":[],"published":{"date-parts":[[2023,7]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Assessment of Droplet Digital PCR for the Detection and Absolute Quantification of Toxoplasma gondii","name":"articletitle","label":"Article Title"},{"value":"The Journal of Molecular Diagnostics","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.jmoldx.2023.03.006","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2023 Association for Molecular Pathology and American Society for Investigative Pathology. Published by Elsevier Inc.","name":"copyright","label":"Copyright"}]}}