{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,29]],"date-time":"2026-10-29T05:33:00Z","timestamp":1793251980871,"version":"4.5.0"},"reference-count":31,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T00:00:00Z","timestamp":1790812800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T00:00:00Z","timestamp":1790812800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T00:00:00Z","timestamp":1792108800000},"content-version":"vor","delay-in-days":15,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100012151","name":"Sanming Project of Medicine in Shenzhen","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100012151","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["clinicalkey.com","clinicalkey.com.au","clinicalkey.es","clinicalkey.fr","clinicalkey.jp","jmdjournal.org","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["The Journal of Molecular Diagnostics"],"published-print":{"date-parts":[[2026,10]]},"DOI":"10.1016\/j.jmoldx.2026.09.005","type":"journal-article","created":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T06:22:30Z","timestamp":1792563750000},"update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":0,"title":["Ultrafast Workflow for Accurate Full-Length ABO Genotyping Using Nanopore Sequencing"],"prefix":"10.1016","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1251-1732","authenticated-orcid":false,"given":"Shuang","family":"Liang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuan","family":"Yao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fan","family":"Wu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tong","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liyan","family":"Sun","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Long","family":"Peng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Li","family":"Ning","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xinyi","family":"Tang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Faming","family":"Zhu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3840-1486","authenticated-orcid":false,"given":"Dawei","family":"Cui","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.jmoldx.2026.09.005_bib1","doi-asserted-by":"crossref","first-page":"207","DOI":"10.1007\/s10719-021-10028-6","article-title":"A historical overview of advances in molecular genetic\/genomic studies of the ABO blood group system","volume":"39","author":"Yamamoto","year":"2022","journal-title":"Glycoconj J"},{"key":"10.1016\/j.jmoldx.2026.09.005_bib2","doi-asserted-by":"crossref","first-page":"48","DOI":"10.21307\/immunohematology-2019-231","article-title":"The ABO blood group system revisited: a review and update","volume":"25","author":"Storry","year":"2009","journal-title":"Immunohematology"},{"key":"10.1016\/j.jmoldx.2026.09.005_bib3","doi-asserted-by":"crossref","first-page":"470","DOI":"10.1186\/s12967-021-03141-5","article-title":"Six splice site variations, three of them novel, in the ABO gene occurring in nine individuals with ABO subtypes","volume":"19","author":"Hong","year":"2021","journal-title":"J Transl Med"},{"key":"10.1016\/j.jmoldx.2026.09.005_bib4","doi-asserted-by":"crossref","first-page":"227","DOI":"10.1111\/j.1423-0410.2007.01013.x","article-title":"Sequences variations in 5\u2032-flanking region of ABO gene and correlation with ABO alleles in the Indigenous Chinese","volume":"94","author":"Yan","year":"2008","journal-title":"Vox Sang"},{"key":"10.1016\/j.jmoldx.2026.09.005_bib5","doi-asserted-by":"crossref","first-page":"1852","DOI":"10.1182\/blood.V88.5.1852.1852","article-title":"ABO glycosyltransferase genotyping by polymerase chain reaction using sequence-specific primers","volume":"88","author":"Gassner","year":"1996","journal-title":"Blood"},{"key":"10.1016\/j.jmoldx.2026.09.005_bib6","doi-asserted-by":"crossref","first-page":"229","DOI":"10.1038\/345229a0","article-title":"Molecular genetic basis of the histo-blood group ABO system","volume":"345","author":"Yamamoto","year":"1990","journal-title":"Nature"},{"key":"10.1016\/j.jmoldx.2026.09.005_bib7","doi-asserted-by":"crossref","first-page":"4337","DOI":"10.1182\/blood.V118.21.4337.4337","article-title":"ABO genotype-phenotype discrepancy due to tri-allelic ABO genotypes and its resolution by cloning-sequencing and chimerism analysis","volume":"118","author":"Lee","year":"2011","journal-title":"Blood"},{"key":"10.1016\/j.jmoldx.2026.09.005_bib8","doi-asserted-by":"crossref","first-page":"2232","DOI":"10.1111\/trf.14654","article-title":"ABO genotyping with next-generation sequencing to resolve heterogeneity in donors with serology discrepancies","volume":"58","author":"Wu","year":"2018","journal-title":"Transfusion"},{"key":"10.1016\/j.jmoldx.2026.09.005_bib9","doi-asserted-by":"crossref","first-page":"2744","DOI":"10.1111\/trf.13797","article-title":"Analysis of exome sequencing data sets reveals structural variation in the coding region of ABO in individuals of African ancestry","volume":"56","author":"Fox","year":"2016","journal-title":"Transfusion"},{"key":"10.1016\/j.jmoldx.2026.09.005_bib10","doi-asserted-by":"crossref","first-page":"374","DOI":"10.1186\/s12864-016-2687-1","article-title":"ABO allele-level frequency estimation based on population-scale genotyping by next generation sequencing","volume":"17","author":"Lang","year":"2016","journal-title":"BMC Genomics"},{"key":"10.1016\/j.jmoldx.2026.09.005_bib11","article-title":"Analysis of the genomic sequence of ABO allele using next-generation sequencing method","volume":"13","author":"He","year":"2022","journal-title":"Front Immunol"},{"key":"10.1016\/j.jmoldx.2026.09.005_bib12","doi-asserted-by":"crossref","first-page":"855","DOI":"10.1016\/j.transci.2017.09.001","article-title":"Gene identification of rare B (A) blood group","volume":"56","author":"Jiao","year":"2017","journal-title":"Transfus Apher Sci"},{"key":"10.1016\/j.jmoldx.2026.09.005_bib13","doi-asserted-by":"crossref","first-page":"878","DOI":"10.1182\/bloodadvances.2022007133","article-title":"Haplotype sequence collection of ABO blood group alleles by long-read sequencing reveals putative A1-diagnostic variants","volume":"7","author":"Gueuning","year":"2023","journal-title":"Blood Adv"},{"key":"10.1016\/j.jmoldx.2026.09.005_bib14","doi-asserted-by":"crossref","first-page":"510","DOI":"10.1093\/clinchem\/hvaf015","article-title":"Comprehensive annotation of complete ABO alleles and resolution of ABO variants by an improved full-length ABO haplotype sequencing","volume":"71","author":"Ying","year":"2025","journal-title":"Clin Chem"},{"key":"10.1016\/j.jmoldx.2026.09.005_bib15","doi-asserted-by":"crossref","first-page":"1196","DOI":"10.1093\/clinchem\/hvac075","article-title":"Rh blood group D antigen genotyping using a portable nanopore-based sequencing device: proof of principle","volume":"68","author":"Tounsi","year":"2022","journal-title":"Clin Chem"},{"key":"10.1016\/j.jmoldx.2026.09.005_bib16","doi-asserted-by":"crossref","first-page":"1348","DOI":"10.1038\/s41587-021-01108-x","article-title":"Nanopore sequencing technology, bioinformatics and applications","volume":"39","author":"Wang","year":"2021","journal-title":"Nat Biotechnol"},{"key":"10.1016\/j.jmoldx.2026.09.005_bib17","doi-asserted-by":"crossref","first-page":"597","DOI":"10.1038\/s41576-020-0236-x","article-title":"Long-read human genome sequencing and its applications","volume":"21","author":"Logsdon","year":"2020","journal-title":"Nat Rev Genet"},{"key":"10.1016\/j.jmoldx.2026.09.005_bib18","doi-asserted-by":"crossref","first-page":"1361","DOI":"10.1016\/j.jgg.2024.09.007","article-title":"Nanopore sequencing: flourishing in its teenage years","volume":"51","author":"Zhang","year":"2024","journal-title":"J Genet Genomics"},{"key":"10.1016\/j.jmoldx.2026.09.005_bib19","doi-asserted-by":"crossref","first-page":"278","DOI":"10.1016\/j.gpb.2015.08.002","article-title":"PacBio sequencing and its applications","volume":"13","author":"Rhoads","year":"2015","journal-title":"Genomics Proteomics Bioinformatics"},{"key":"10.1016\/j.jmoldx.2026.09.005_bib20","doi-asserted-by":"crossref","first-page":"180","DOI":"10.1016\/j.ajhg.2021.12.003","article-title":"Accurate long-read sequencing allows assembly of the duplicated RHD and RHCE genes harboring variants relevant to blood transfusion","volume":"109","author":"Zhang","year":"2022","journal-title":"Am J Hum Genet"},{"key":"10.1016\/j.jmoldx.2026.09.005_bib21","doi-asserted-by":"crossref","first-page":"23","DOI":"10.1159\/000504584","article-title":"Defining blood group gene reference alleles by long-read sequencing: proof of concept in the ACKR1 gene encoding the duffy antigens","volume":"47","author":"Fichou","year":"2020","journal-title":"Transfus Med Hemother"},{"key":"10.1016\/j.jmoldx.2026.09.005_bib22","doi-asserted-by":"crossref","first-page":"341","DOI":"10.1186\/s12864-026-12701-2","article-title":"Full-length haplotype reconstruction of CD36 by long-read sequencing: uncovers a novel structural variant","volume":"27","author":"Liang","year":"2026","journal-title":"BMC Genomics"},{"key":"10.1016\/j.jmoldx.2026.09.005_bib23","series-title":"Technical Manual","first-page":"305","article-title":"ABO and other carbohydrate blood group systems","author":"Arthur","year":"2023"},{"key":"10.1016\/j.jmoldx.2026.09.005_bib24","doi-asserted-by":"crossref","first-page":"222","DOI":"10.1186\/s13059-023-03053-1","article-title":"HycDemux: a hybrid unsupervised approach for accurate barcoded sample demultiplexing in nanopore sequencing","volume":"24","author":"Han","year":"2023","journal-title":"Genome Biol"},{"key":"10.1016\/j.jmoldx.2026.09.005_bib25","doi-asserted-by":"crossref","first-page":"271","DOI":"10.1186\/s13104-023-06532-7","article-title":"PacBio long read-assembled draft genome of Pythium insidiosum strain Pi-S isolated from a Thai patient with pythiosis","volume":"16","author":"Krajaejun","year":"2023","journal-title":"BMC Res Notes"},{"key":"10.1016\/j.jmoldx.2026.09.005_bib26","doi-asserted-by":"crossref","DOI":"10.1155\/2020\/7231205","article-title":"DeepVariant-on-spark: small-scale genome analysis using a cloud-based computing framework","volume":"2020","author":"Huang","year":"2020","journal-title":"Comput Math Methods Med"},{"key":"10.1016\/j.jmoldx.2026.09.005_bib27","doi-asserted-by":"crossref","DOI":"10.1016\/j.jbc.2023.104766","article-title":"Construction and validation of customized genomes for human and mouse ribosomal DNA mapping","volume":"299","author":"George","year":"2023","journal-title":"J Biol Chem"},{"key":"10.1016\/j.jmoldx.2026.09.005_bib28","doi-asserted-by":"crossref","DOI":"10.1155\/humu\/5597086","article-title":"Comparative analysis of FUT1 and FUT2 haplotype diversity in multi-ethnic populations via long-read sequencing","volume":"2025","author":"Liang","year":"2025","journal-title":"Hum Mutat"},{"key":"10.1016\/j.jmoldx.2026.09.005_bib29","doi-asserted-by":"crossref","first-page":"160","DOI":"10.1159\/000501862","article-title":"Molecular basis of ABO variants including identification of 16 novel ABO subgroup alleles in Chinese Han population","volume":"47","author":"Ying","year":"2020","journal-title":"Transfus Med Hemother"},{"key":"10.1016\/j.jmoldx.2026.09.005_bib30","doi-asserted-by":"crossref","first-page":"509","DOI":"10.1097\/MOH.0000000000000469","article-title":"The role of genomics in transfusion medicine","volume":"25","author":"Wheeler","year":"2018","journal-title":"Curr Opin Hematol"},{"key":"10.1016\/j.jmoldx.2026.09.005_bib31","doi-asserted-by":"crossref","first-page":"721","DOI":"10.1111\/trf.16835","article-title":"Novel or not? reference alleles, genes, and genomes to unmask the true nature of the ABO\u2217 AW. 10 allele associated with weak A phenotype","volume":"62","author":"Gassner","year":"2022","journal-title":"Transfusion"}],"container-title":["The Journal of Molecular Diagnostics"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1525157826001820?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1525157826001820?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,10,29]],"date-time":"2026-10-29T05:02:24Z","timestamp":1793250144000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S1525157826001820"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,10]]},"references-count":31,"alternative-id":["S1525157826001820"],"URL":"https:\/\/doi.org\/10.1016\/j.jmoldx.2026.09.005","relation":{},"ISSN":["1525-1578"],"issn-type":[{"value":"1525-1578","type":"print"}],"subject":[],"published":{"date-parts":[[2026,10]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Ultrafast Workflow for Accurate Full-Length ABO Genotyping Using Nanopore Sequencing","name":"articletitle","label":"Article Title"},{"value":"The Journal of Molecular Diagnostics","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.jmoldx.2026.09.005","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2026 The Author(s). Published by Elsevier Inc. on behalf of the Association for Molecular Pathology and American Society for Investigative Pathology.","name":"copyright","label":"Copyright"}]}}