{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T07:57:13Z","timestamp":1790841433303,"version":"4.1.0"},"reference-count":38,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T00:00:00Z","timestamp":1790812800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T00:00:00Z","timestamp":1790812800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T00:00:00Z","timestamp":1790812800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-017"},{"start":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T00:00:00Z","timestamp":1790812800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T00:00:00Z","timestamp":1790812800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-012"},{"start":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T00:00:00Z","timestamp":1790812800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T00:00:00Z","timestamp":1790812800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-004"}],"funder":[{"DOI":"10.13039\/501100003725","name":"NRF","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003725","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Journal of Magnetic Resonance"],"published-print":{"date-parts":[[2026,10]]},"DOI":"10.1016\/j.jmr.2026.108161","type":"journal-article","created":{"date-parts":[[2026,8,26]],"date-time":"2026-08-26T23:34:39Z","timestamp":1787787279000},"page":"108161","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":0,"special_numbering":"C","title":["Comparative Kd analysis of the interaction between aggregation-prone Nup153 3\u20132 and KapN"],"prefix":"10.1016","volume":"391","author":[{"given":"Shinyeong","family":"Lee","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"ByeongJin","family":"Cho","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jonghyuk","family":"Im","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Junseock","family":"Koh","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jung Ho","family":"Lee","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.jmr.2026.108161_bb0005","doi-asserted-by":"crossref","DOI":"10.7554\/eLife.57264","article-title":"How to measure and evaluate binding affinities","volume":"9","author":"Jarmoskaite","year":"2020","journal-title":"eLife"},{"key":"10.1016\/j.jmr.2026.108161_bb0010","doi-asserted-by":"crossref","first-page":"197","DOI":"10.1038\/nrm1589","article-title":"Intrinsically unstructured proteins and their functions","volume":"6","author":"Dyson","year":"2005","journal-title":"Nat. Rev. Mol. Cell Biol."},{"key":"10.1016\/j.jmr.2026.108161_bb0015","doi-asserted-by":"crossref","first-page":"333","DOI":"10.1146\/annurev.biochem.75.101304.123901","article-title":"Protein misfolding, functional amyloid, and human disease","volume":"75","author":"Chiti","year":"2006","journal-title":"Annu. Rev. Biochem."},{"key":"10.1016\/j.jmr.2026.108161_bb0020","doi-asserted-by":"crossref","first-page":"99","DOI":"10.1016\/S0092-8674(00)00014-3","article-title":"Structural basis for the interaction between FxFG nucleoporin repeats and importin-\u03b2 in nuclear trafficking","volume":"102","author":"Bayliss","year":"2000","journal-title":"Cell"},{"key":"10.1016\/j.jmr.2026.108161_bb0025","doi-asserted-by":"crossref","first-page":"423","DOI":"10.1242\/jcs.083246","article-title":"The nuclear pore complex-structure and function at a glance","volume":"128","author":"Kabachinski","year":"2015","journal-title":"J. Cell Sci."},{"key":"10.1016\/j.jmr.2026.108161_bb0030","doi-asserted-by":"crossref","first-page":"12509","DOI":"10.1021\/jacs.1c00811","article-title":"Thermodynamic models for assembly of intrinsically disordered protein hubs with multiple interaction partners","volume":"143","author":"Cho","year":"2021","journal-title":"J. Am. Chem. Soc."},{"key":"10.1016\/j.jmr.2026.108161_bb0035","doi-asserted-by":"crossref","first-page":"20.12.1","DOI":"10.1002\/0471140864.ps2012s71","article-title":"Overview of current methods in sedimentation velocity and sedimentation equilibrium analytical ultracentrifugation","volume":"71","author":"Zhao","year":"2013","journal-title":"Curr. Protoc. Protein Sci."},{"key":"10.1016\/j.jmr.2026.108161_bb0040","doi-asserted-by":"crossref","first-page":"144","DOI":"10.3390\/ijms17020144","article-title":"Insights into protein\u2013ligand interactions: mechanisms, models, and methods","volume":"17","author":"Du","year":"2016","journal-title":"Int. J. Mol. Sci."},{"key":"10.1016\/j.jmr.2026.108161_bb0045","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0217897","article-title":"Interactions of nuclear transport factors and surface-conjugated FG nucleoporins: insights and limitations","volume":"14","author":"Hayama","year":"2019","journal-title":"PloS One"},{"key":"10.1016\/j.jmr.2026.108161_bb0050","doi-asserted-by":"crossref","first-page":"4555","DOI":"10.1074\/jbc.AC117.001649","article-title":"Thermodynamic characterization of the multivalent interactions underlying rapid and selective translocation through the nuclear pore complex","volume":"293","author":"Hayama","year":"2018","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.jmr.2026.108161_bb0055","doi-asserted-by":"crossref","first-page":"680","DOI":"10.1002\/bkcs.70036","article-title":"Dynamic protein interactions probed by NMR spectroscopy","volume":"46","author":"Lee","year":"2025","journal-title":"Bull. Korean Chem. Soc."},{"key":"10.1016\/j.jmr.2026.108161_bb0060","doi-asserted-by":"crossref","first-page":"8148","DOI":"10.1021\/ja3001419","article-title":"Studying \u201cinvisible\u201d excited protein states in slow exchange with a major state conformation","volume":"134","author":"Vallurupalli","year":"2012","journal-title":"J. Am. Chem. Soc."},{"key":"10.1016\/j.jmr.2026.108161_bb0065","doi-asserted-by":"crossref","first-page":"204","DOI":"10.1016\/S0076-6879(01)39315-1","article-title":"Nuclear magnetic resonance methods for quantifying microsecond-to-millisecond motions in biological macromolecules","volume":"339","author":"Palmer","year":"2001","journal-title":"Methods Enzymol."},{"key":"10.1016\/j.jmr.2026.108161_bb0070","doi-asserted-by":"crossref","first-page":"990","DOI":"10.1021\/ed101169t","article-title":"Saturation-transfer difference (STD) NMR: a simple and fast method for ligand screening and characterization of protein binding","volume":"88","author":"Viegas","year":"2011","journal-title":"J. Chem. Educ."},{"key":"10.1016\/j.jmr.2026.108161_bb0075","doi-asserted-by":"crossref","first-page":"65","DOI":"10.1023\/A:1008354229396","article-title":"Identification of compounds with binding affinity to proteins via magnetization transfer from bulk water","volume":"18","author":"Dalvit","year":"2000","journal-title":"J. Biomol. NMR"},{"key":"10.1016\/j.jmr.2026.108161_bb0080","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.pnmrs.2013.02.001","article-title":"Using chemical shift perturbation to characterise ligand binding","volume":"73","author":"Williamson","year":"2013","journal-title":"Prog. Nucl. Magn. Reson. Spectrosc."},{"key":"10.1016\/j.jmr.2026.108161_bb0085","first-page":"125","article-title":"Protein-ligand interaction investigated by HSQC titration study","volume":"22","author":"Lee","year":"2018","journal-title":"J. Korean Magn. Reson. Soc."},{"key":"10.1016\/j.jmr.2026.108161_bb0090","doi-asserted-by":"crossref","first-page":"24826","DOI":"10.1038\/srep24826","article-title":"Two-dimensional NMR lineshape analysis","volume":"6","author":"Waudby","year":"2016","journal-title":"Sci. Rep."},{"key":"10.1016\/j.jmr.2026.108161_bb0095","doi-asserted-by":"crossref","first-page":"6151","DOI":"10.1016\/S0040-4020(00)00492-0","article-title":"Determination of association constants (Ka) from solution NMR data","volume":"56","author":"Fielding","year":"2000","journal-title":"Tetrahedron"},{"key":"10.1016\/j.jmr.2026.108161_bb0100","doi-asserted-by":"crossref","first-page":"1361","DOI":"10.1016\/j.cell.2015.05.013","article-title":"Allosteric regulation in gating the central channel of the nuclear pore complex","volume":"161","author":"Koh","year":"2015","journal-title":"Cell"},{"key":"10.1016\/j.jmr.2026.108161_bb0105","doi-asserted-by":"crossref","first-page":"277","DOI":"10.1007\/BF00197809","article-title":"NMRPipe: a multidimensional spectral processing system based on UNIX pipes","volume":"6","author":"Delaglio","year":"1995","journal-title":"J. Biomol. NMR"},{"key":"10.1016\/j.jmr.2026.108161_bb0110","doi-asserted-by":"crossref","first-page":"101","DOI":"10.1007\/s10858-016-0072-7","article-title":"Sparse multidimensional iterative lineshape-enhanced (SMILE) reconstruction of both non-uniformly sampled and conventional NMR data","volume":"68","author":"Ying","year":"2017","journal-title":"J. Biomol. NMR"},{"key":"10.1016\/j.jmr.2026.108161_bb0115","first-page":"432","article-title":"Improved 3D triple-resonance NMR techniques applied to a 31 kDa protein","volume":"96","author":"Grzesiek","year":"1992","journal-title":"J. Magn. Reson."},{"key":"10.1016\/j.jmr.2026.108161_bb0120","first-page":"213","article-title":"A constant-time three-dimensional triple-resonance pulse scheme to correlate intraresidue 1HN, 15N, and 13C' chemical shifts in 15N 13C-labelled proteins","volume":"97","author":"Clubb","year":"1992","journal-title":"J. Magn. Reson."},{"key":"10.1016\/j.jmr.2026.108161_bb0125","first-page":"201","article-title":"An efficient experiment for sequential backbone assignment of medium-sized isotopically enriched proteins","volume":"99","author":"Grzesiek","year":"1992","journal-title":"J. Magn. Reson."},{"key":"10.1016\/j.jmr.2026.108161_bb0130","doi-asserted-by":"crossref","first-page":"6291","DOI":"10.1021\/ja00042a003","article-title":"Correlating backbone amide and side chain resonances in larger proteins by multiple relayed triple resonance NMR","volume":"114","author":"Grzesiek","year":"1992","journal-title":"J. Am. Chem. Soc."},{"key":"10.1016\/j.jmr.2026.108161_bb0135","doi-asserted-by":"crossref","first-page":"333","DOI":"10.1006\/jmrb.1993.1053","article-title":"A gradient-enhanced HCCH-TOCSY experiment for recording side-chain 1H and 13C correlations in H2O samples of proteins","volume":"101","author":"Kay","year":"1993","journal-title":"J. Magn. Reson. B"},{"key":"10.1016\/j.jmr.2026.108161_bb0140","doi-asserted-by":"crossref","first-page":"btad066","DOI":"10.1093\/bioinformatics\/btad066","article-title":"NMRtist: an online platform for automated biomolecular NMR spectra analysis","volume":"39","author":"Klukowski","year":"2023","journal-title":"Bioinformatics"},{"key":"10.1016\/j.jmr.2026.108161_bb0145","doi-asserted-by":"crossref","first-page":"3041","DOI":"10.1093\/bioinformatics\/btab180","article-title":"POKY: a software suite for multidimensional NMR and 3D structure calculation of biomolecules","volume":"37","author":"Lee","year":"2021","journal-title":"Bioinformatics"},{"key":"10.1016\/j.jmr.2026.108161_bb0150","doi-asserted-by":"crossref","first-page":"288","DOI":"10.1063\/1.1695690","article-title":"Spin diffusion measurements: spin echoes in the presence of a time-dependent field gradient","volume":"42","author":"Stejskal","year":"1965","journal-title":"J. Chem. Phys."},{"key":"10.1016\/j.jmr.2026.108161_bb0155","unstructured":"J. Lee, J.H. Lee, Deriving the Stejskal\u2013Tanner equation in diffusion NMR using Mathematica., J. Kor. Magn. Reson. Soc. 30 (2026) 1\u201322."},{"key":"10.1016\/j.jmr.2026.108161_bb0160","doi-asserted-by":"crossref","first-page":"685","DOI":"10.1021\/j100624a025","article-title":"Self-diffusion in normal and heavy water in the range 1-45 deg","volume":"77","author":"Mills","year":"2002","journal-title":"J. Phys. Chem."},{"key":"10.1016\/j.jmr.2026.108161_bb0165","doi-asserted-by":"crossref","first-page":"14016","DOI":"10.1038\/s41598-019-50502-8","article-title":"Investigation of the core binding regions of human Werner syndrome and Fanconi anemia group J helicases on replication protein a","volume":"9","author":"Yeom","year":"2019","journal-title":"Sci. Rep."},{"key":"10.1016\/j.jmr.2026.108161_bb0170","doi-asserted-by":"crossref","first-page":"275","DOI":"10.1007\/s10858-007-9197-z","article-title":"Combined chemical shift changes and amino acid specific chemical shift mapping of protein-protein interactions","volume":"39","author":"Schumann","year":"2007","journal-title":"J. Biomol. NMR"},{"key":"10.1016\/j.jmr.2026.108161_bb0175","doi-asserted-by":"crossref","first-page":"95","DOI":"10.1007\/s10858-019-00297-7","article-title":"Two-dimensional NMR lineshape analysis of single, multiple, zero and double quantum correlation experiments","volume":"74","author":"Waudby","year":"2020","journal-title":"J. Biomol. NMR"},{"key":"10.1016\/j.jmr.2026.108161_bb0180","doi-asserted-by":"crossref","first-page":"549","DOI":"10.1002\/andp.19053220806","article-title":"On the movement of small particles suspended in a stationary liquid demanded by the molecular-kinetic theory of heat","volume":"17","author":"Einstein","year":"1905","journal-title":"Ann. Phys."},{"key":"10.1016\/j.jmr.2026.108161_bb0185","doi-asserted-by":"crossref","first-page":"131","DOI":"10.1016\/0003-2697(89)90213-3","article-title":"Rapid measurement of binding constants and heats of binding using a new titration calorimeter","volume":"179","author":"Wiseman","year":"1989","journal-title":"Anal. Biochem."},{"key":"10.1016\/j.jmr.2026.108161_bb0190","doi-asserted-by":"crossref","first-page":"734","DOI":"10.1016\/j.cell.2015.09.047","article-title":"Plasticity of an ultrafast interaction between nucleoporins and nuclear transport receptors","volume":"163","author":"Milles","year":"2015","journal-title":"Cell"}],"container-title":["Journal of Magnetic Resonance"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1090780726001503?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1090780726001503?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T07:09:50Z","timestamp":1790838590000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S1090780726001503"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,10]]},"references-count":38,"alternative-id":["S1090780726001503"],"URL":"https:\/\/doi.org\/10.1016\/j.jmr.2026.108161","relation":{},"ISSN":["1090-7807"],"issn-type":[{"value":"1090-7807","type":"print"}],"subject":[],"published":{"date-parts":[[2026,10]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Comparative Kd analysis of the interaction between aggregation-prone Nup153 3\u20132 and KapN","name":"articletitle","label":"Article Title"},{"value":"Journal of Magnetic Resonance","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.jmr.2026.108161","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2026 Elsevier Inc. All rights are reserved, including those for text and data mining, AI training, and similar technologies.","name":"copyright","label":"Copyright"}],"article-number":"108161"}}