{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T17:03:35Z","timestamp":1792602215741,"version":"4.5.0"},"reference-count":29,"publisher":"Elsevier BV","issue":"1","license":[{"start":{"date-parts":[[2008,3,1]],"date-time":"2008-03-01T00:00:00Z","timestamp":1204329600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2008,3,1]],"date-time":"2008-03-01T00:00:00Z","timestamp":1204329600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2008,3,1]],"date-time":"2008-03-01T00:00:00Z","timestamp":1204329600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-017"},{"start":{"date-parts":[[2008,3,1]],"date-time":"2008-03-01T00:00:00Z","timestamp":1204329600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2008,3,1]],"date-time":"2008-03-01T00:00:00Z","timestamp":1204329600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-012"},{"start":{"date-parts":[[2008,3,1]],"date-time":"2008-03-01T00:00:00Z","timestamp":1204329600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2008,3,1]],"date-time":"2008-03-01T00:00:00Z","timestamp":1204329600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-004"}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Journal of Neuroscience Methods"],"published-print":{"date-parts":[[2008,3]]},"DOI":"10.1016\/j.jneumeth.2007.11.011","type":"journal-article","created":{"date-parts":[[2007,12,5]],"date-time":"2007-12-05T14:16:23Z","timestamp":1196864183000},"page":"1-7","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":117,"title":["Stable in vivo imaging of densely populated glia, axons and blood vessels in the mouse spinal cord using two-photon microscopy"],"prefix":"10.1016","volume":"169","author":[{"given":"Dimitrios","family":"Davalos","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jae K.","family":"Lee","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"W. Bryan","family":"Smith","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Brendan","family":"Brinkman","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mark H.","family":"Ellisman","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Binhai","family":"Zheng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katerina","family":"Akassoglou","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.jneumeth.2007.11.011_bib1","doi-asserted-by":"crossref","first-page":"129","DOI":"10.1113\/jphysiol.2001.013084","article-title":"Effects of daily spontaneous running on the electrophysiological properties of hindlimb motoneurones in rats","volume":"540","author":"Beaumont","year":"2002","journal-title":"J Physiol"},{"key":"10.1016\/j.jneumeth.2007.11.011_bib2","doi-asserted-by":"crossref","first-page":"858","DOI":"10.1523\/JNEUROSCI.21-03-00858.2001","article-title":"Growth arrest of individual senile plaques in a model of Alzheimer's disease observed by in vivo multiphoton microscopy","volume":"21","author":"Christie","year":"2001","journal-title":"J Neurosci"},{"key":"10.1016\/j.jneumeth.2007.11.011_bib3","doi-asserted-by":"crossref","first-page":"752","DOI":"10.1038\/nn1472","article-title":"ATP mediates rapid microglial response to local brain injury in vivo","volume":"8","author":"Davalos","year":"2005","journal-title":"Nat Neurosci"},{"key":"10.1016\/j.jneumeth.2007.11.011_bib4","doi-asserted-by":"crossref","first-page":"73","DOI":"10.1126\/science.2321027","article-title":"Two-photon laser scanning fluorescence microscopy","volume":"248","author":"Denk","year":"1990","journal-title":"Science"},{"key":"10.1016\/j.jneumeth.2007.11.011_bib5","doi-asserted-by":"crossref","first-page":"41","DOI":"10.1016\/S0896-6273(00)00084-2","article-title":"Imaging neuronal subsets in transgenic mice expressing multiple spectral variants of GFP","volume":"28","author":"Feng","year":"2000","journal-title":"Neuron"},{"key":"10.1016\/j.jneumeth.2007.11.011_bib6","doi-asserted-by":"crossref","first-page":"625","DOI":"10.1113\/jphysiol.1955.sp005432","article-title":"Potentials recorded from the spinal cord with microelectrodes","volume":"130","author":"Frank","year":"1955","journal-title":"J Physiol"},{"key":"10.1016\/j.jneumeth.2007.11.011_bib7","doi-asserted-by":"crossref","first-page":"497","DOI":"10.1038\/nri1884","article-title":"Dynamic imaging of the immune system: progress, pitfalls and promise","volume":"6","author":"Germain","year":"2006","journal-title":"Nat Rev Immunol"},{"key":"10.1016\/j.jneumeth.2007.11.011_bib8","doi-asserted-by":"crossref","first-page":"489","DOI":"10.1016\/j.nurx.2006.07.005","article-title":"Two-photon imaging of synaptic plasticity and pathology in the living mouse brain","volume":"3","author":"Grutzendler","year":"2006","journal-title":"NeuroRx"},{"key":"10.1016\/j.jneumeth.2007.11.011_bib9","doi-asserted-by":"crossref","first-page":"812","DOI":"10.1038\/nature01276","article-title":"Long-term dendritic spine stability in the adult cortex","volume":"420","author":"Grutzendler","year":"2002","journal-title":"Nature"},{"key":"10.1016\/j.jneumeth.2007.11.011_bib10","doi-asserted-by":"crossref","first-page":"932","DOI":"10.1038\/nmeth818","article-title":"Deep tissue two-photon microscopy","volume":"2","author":"Helmchen","year":"2005","journal-title":"Nat Methods"},{"key":"10.1016\/j.jneumeth.2007.11.011_bib11","doi-asserted-by":"crossref","first-page":"4106","DOI":"10.1128\/MCB.20.11.4106-4114.2000","article-title":"Analysis of fractalkine receptor CX3CR1 function by targeted deletion and green fluorescent protein reporter gene insertion","volume":"20","author":"Jung","year":"2000","journal-title":"Mol Cell Biol"},{"key":"10.1016\/j.jneumeth.2007.11.011_bib12","doi-asserted-by":"crossref","first-page":"1805","DOI":"10.1084\/jem.20050011","article-title":"Live imaging of effector cell trafficking and autoantigen recognition within the unfolding autoimmune encephalomyelitis lesion","volume":"201","author":"Kawakami","year":"2005","journal-title":"J Exp Med"},{"key":"10.1016\/j.jneumeth.2007.11.011_bib13","doi-asserted-by":"crossref","first-page":"572","DOI":"10.1038\/nm1229","article-title":"In vivo imaging of axonal degeneration and regeneration in the injured spinal cord","volume":"11","author":"Kerschensteiner","year":"2005","journal-title":"Nat Med"},{"key":"10.1016\/j.jneumeth.2007.11.011_bib14","doi-asserted-by":"crossref","first-page":"1243","DOI":"10.1038\/ni1139","article-title":"Visualizing dendritic cell networks in vivo","volume":"5","author":"Lindquist","year":"2004","journal-title":"Nat Immunol"},{"key":"10.1016\/j.jneumeth.2007.11.011_bib15","doi-asserted-by":"crossref","first-page":"449","DOI":"10.1038\/nrn1905","article-title":"In vivo imaging of the diseased nervous system","volume":"7","author":"Misgeld","year":"2006","journal-title":"Nat Rev Neurosci"},{"key":"10.1016\/j.jneumeth.2007.11.011_bib16","doi-asserted-by":"crossref","first-page":"263","DOI":"10.1038\/nprot.2007.24","article-title":"In vivo imaging of single axons in the mouse spinal cord","volume":"2","author":"Misgeld","year":"2007","journal-title":"Nat Protoc"},{"key":"10.1016\/j.jneumeth.2007.11.011_bib17","doi-asserted-by":"crossref","first-page":"1314","DOI":"10.1126\/science.1110647","article-title":"Resting microglial cells are highly dynamic surveillants of brain parenchyma in vivo","volume":"308","author":"Nimmerjahn","year":"2005","journal-title":"Science"},{"key":"10.1016\/j.jneumeth.2007.11.011_bib18","doi-asserted-by":"crossref","first-page":"83","DOI":"10.1038\/nature05573","article-title":"In vivo imaging of germinal centres reveals a dynamic open structure","volume":"446","author":"Schwickert","year":"2007","journal-title":"Nature"},{"key":"10.1016\/j.jneumeth.2007.11.011_bib19","doi-asserted-by":"crossref","first-page":"707","DOI":"10.1038\/ni1210","article-title":"Stable T cell-dendritic cell interactions precede the development of both tolerance and immunity in vivo","volume":"6","author":"Shakhar","year":"2005","journal-title":"Nat Immunol"},{"key":"10.1016\/j.jneumeth.2007.11.011_bib20","doi-asserted-by":"crossref","first-page":"823","DOI":"10.1016\/j.neuron.2006.05.019","article-title":"Principles of two-photon excitation microscopy and its applications to neuroscience","volume":"50","author":"Svoboda","year":"2006","journal-title":"Neuron"},{"key":"10.1016\/j.jneumeth.2007.11.011_bib21","doi-asserted-by":"crossref","first-page":"161","DOI":"10.1038\/385161a0","article-title":"In vivo dendritic calcium dynamics in neocortical pyramidal neurons","volume":"385","author":"Svoboda","year":"1997","journal-title":"Nature"},{"key":"10.1016\/j.jneumeth.2007.11.011_bib22","doi-asserted-by":"crossref","first-page":"505","DOI":"10.1084\/jem.20050783","article-title":"Regulatory T cells inhibit stable contacts between CD4+ T cells and dendritic cells in vivo","volume":"203","author":"Tadokoro","year":"2006","journal-title":"J Exp Med"},{"key":"10.1016\/j.jneumeth.2007.11.011_bib23","doi-asserted-by":"crossref","first-page":"40","DOI":"10.1196\/annals.1379.004","article-title":"Two-photon imaging of astrocytic Ca2+ signaling and the microvasculature in experimental mice models of Alzheimer's disease","volume":"1097","author":"Takano","year":"2007","journal-title":"Ann N Y Acad Sci"},{"key":"10.1016\/j.jneumeth.2007.11.011_bib24","doi-asserted-by":"crossref","first-page":"788","DOI":"10.1038\/nature01273","article-title":"Long-term in vivo imaging of experience-dependent synaptic plasticity in adult cortex","volume":"420","author":"Trachtenberg","year":"2002","journal-title":"Nature"},{"key":"10.1016\/j.jneumeth.2007.11.011_bib25","doi-asserted-by":"crossref","first-page":"1181","DOI":"10.1038\/nn1335","article-title":"Fibrillar amyloid deposition leads to local synaptic abnormalities and breakage of neuronal branches","volume":"7","author":"Tsai","year":"2004","journal-title":"Nat Neurosci"},{"key":"10.1016\/j.jneumeth.2007.11.011_bib26","doi-asserted-by":"crossref","first-page":"509","DOI":"10.1146\/annurev.biochem.67.1.509","article-title":"The green fluorescent protein","volume":"67","author":"Tsien","year":"1998","journal-title":"Annu Rev Biochem"},{"key":"10.1016\/j.jneumeth.2007.11.011_bib27","doi-asserted-by":"crossref","first-page":"816","DOI":"10.1038\/nn1703","article-title":"Astrocytic Ca2+ signaling evoked by sensory stimulation in vivo","volume":"9","author":"Wang","year":"2006","journal-title":"Nat Neurosci"},{"key":"10.1016\/j.jneumeth.2007.11.011_bib28","doi-asserted-by":"crossref","first-page":"549","DOI":"10.1038\/nn1883","article-title":"Choice of cranial window type for in vivo imaging affects dendritic spine turnover in the cortex","volume":"10","author":"Xu","year":"2007","journal-title":"Nat Neurosci"},{"key":"10.1016\/j.jneumeth.2007.11.011_bib29","doi-asserted-by":"crossref","first-page":"213","DOI":"10.1016\/S0896-6273(03)00225-3","article-title":"Lack of enhanced spinal regeneration in Nogo-deficient mice","volume":"38","author":"Zheng","year":"2003","journal-title":"Neuron"}],"container-title":["Journal of Neuroscience Methods"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0165027007005596?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0165027007005596?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,9,28]],"date-time":"2025-09-28T00:13:46Z","timestamp":1759018426000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0165027007005596"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2008,3]]},"references-count":29,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2008,3]]}},"alternative-id":["S0165027007005596"],"URL":"https:\/\/doi.org\/10.1016\/j.jneumeth.2007.11.011","relation":{},"ISSN":["0165-0270"],"issn-type":[{"value":"0165-0270","type":"print"}],"subject":[],"published":{"date-parts":[[2008,3]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Stable in vivo imaging of densely populated glia, axons and blood vessels in the mouse spinal cord using two-photon microscopy","name":"articletitle","label":"Article Title"},{"value":"Journal of Neuroscience Methods","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.jneumeth.2007.11.011","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"Copyright \u00a9 2007 Elsevier B.V. All rights reserved.","name":"copyright","label":"Copyright"}]}}