{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T13:57:55Z","timestamp":1790517475955,"version":"4.1.0"},"reference-count":18,"publisher":"Elsevier BV","issue":"2","license":[{"start":{"date-parts":[[2013,9,1]],"date-time":"2013-09-01T00:00:00Z","timestamp":1377993600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2013,9,1]],"date-time":"2013-09-01T00:00:00Z","timestamp":1377993600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"}],"funder":[{"DOI":"10.13039\/501100000925","name":"National Health and Medical Research Council","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100000925","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100001001","name":"Alzheimer's Australia","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100001001","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Select Foundation"},{"name":"Tasmanian Masonic Medical Research Foundation"},{"name":"Motor Neuron Disease Research Institute of Australia"}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Journal of Neuroscience Methods"],"published-print":{"date-parts":[[2013,9]]},"DOI":"10.1016\/j.jneumeth.2013.06.002","type":"journal-article","created":{"date-parts":[[2013,6,14]],"date-time":"2013-06-14T15:04:02Z","timestamp":1371222242000},"page":"164-169","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":114,"title":["Microfluidic primary culture model of the lower motor neuron\u2013neuromuscular junction circuit"],"prefix":"10.1016","volume":"218","author":[{"given":"Katherine A.","family":"Southam","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anna E.","family":"King","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Catherine A.","family":"Blizzard","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Graeme H.","family":"McCormack","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tracey C.","family":"Dickson","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.jneumeth.2013.06.002_bib0005","doi-asserted-by":"crossref","first-page":"4516","DOI":"10.1073\/pnas.74.10.4516","article-title":"Local control of neurite development by nerve growth factor","volume":"74","author":"Campenot","year":"1977","journal-title":"Proc Natl Acad Sci U S A"},{"key":"10.1016\/j.jneumeth.2013.06.002_bib0010","doi-asserted-by":"crossref","first-page":"26","DOI":"10.1002\/(SICI)1097-0029(20000401)49:1<26::AID-JEMT4>3.0.CO;2-8","article-title":"Rodent nerve\u2013muscle cell culture system for studies of neuromuscular junction development: refinements and applications","volume":"49","author":"Daniels","year":"2000","journal-title":"Microsc Res Tech"},{"key":"10.1016\/j.jneumeth.2013.06.002_bib0015","doi-asserted-by":"crossref","first-page":"9602","DOI":"10.1016\/j.biomaterials.2011.09.014","article-title":"Neuromuscular junction formation between human stem cell-derived motoneurons and human skeletal muscle in a defined system","volume":"32","author":"Guo","year":"2011","journal-title":"Biomaterials"},{"key":"10.1016\/j.jneumeth.2013.06.002_bib0020","doi-asserted-by":"crossref","DOI":"10.1042\/AN20110031","article-title":"Chronic excitotoxin-induced axon degeneration in a compartmented neuronal culture model","volume":"4","author":"Hosie","year":"2012","journal-title":"ASN Neuro"},{"key":"10.1016\/j.jneumeth.2013.06.002_bib0025","doi-asserted-by":"crossref","first-page":"369","DOI":"10.1002\/(SICI)1097-4695(19990215)38:3<369::AID-NEU6>3.0.CO;2-Q","article-title":"The thrombin receptor mediates functional activity-dependent neuromuscular synapse reduction via protein kinase C activation in vitro","volume":"38","author":"Jia","year":"1999","journal-title":"J Neurobiol"},{"key":"10.1016\/j.jneumeth.2013.06.002_bib0030","doi-asserted-by":"crossref","first-page":"659","DOI":"10.1002\/dvdy.20589","article-title":"Presence of neurotrophic factors in skeletal muscle correlates with survival of spinal cord motor neurons","volume":"234","author":"Kablar","year":"2005","journal-title":"Dev Dyn"},{"key":"10.1016\/j.jneumeth.2013.06.002_bib0035","doi-asserted-by":"crossref","first-page":"2151","DOI":"10.1111\/j.1460-9568.2007.05845.x","article-title":"Excitotoxicity mediated by non-NMDA receptors causes distal axonopathy in long-term cultured spinal motor neurons","volume":"26","author":"King","year":"2007","journal-title":"Eur J Neurosci"},{"key":"10.1016\/j.jneumeth.2013.06.002_bib0040","doi-asserted-by":"crossref","first-page":"459","DOI":"10.1016\/j.neurobiolaging.2009.04.004","article-title":"Neuron\u2013glia interactions underlie ALS-like axonal cytoskeletal pathology","volume":"32","author":"King","year":"2011","journal-title":"Neurobiol Aging"},{"key":"10.1016\/j.jneumeth.2013.06.002_bib0045","doi-asserted-by":"crossref","first-page":"239","DOI":"10.1002\/cne.1312","article-title":"Differentiation of glial cells and motor neurons during the formation of neuromuscular junctions in cocultures of rat spinal cord explant and human muscle","volume":"438","author":"Mars","year":"2001","journal-title":"J Comp Neurol"},{"key":"10.1016\/j.jneumeth.2013.06.002_bib0050","doi-asserted-by":"crossref","first-page":"752","DOI":"10.1016\/j.tins.2012.09.001","article-title":"New perspectives on neuronal development via microfluidic environments","volume":"35","author":"Millet","year":"2012","journal-title":"Trends Neurosci"},{"key":"10.1016\/j.jneumeth.2013.06.002_bib0055","doi-asserted-by":"crossref","first-page":"4545","DOI":"10.1074\/jbc.275.7.4545","article-title":"Stimulation of slow skeletal muscle fiber gene expression by calcineurin in vivo","volume":"275","author":"Naya","year":"2000","journal-title":"J Biol Chem"},{"key":"10.1016\/j.jneumeth.2013.06.002_bib0060","doi-asserted-by":"crossref","first-page":"1517","DOI":"10.1002\/neu.480241106","article-title":"Synapse elimination from the mouse neuromuscular junction in vitro: a non-Hebbian activity-dependent process","volume":"24","author":"Nelson","year":"1993","journal-title":"J Neurobiol"},{"key":"10.1016\/j.jneumeth.2013.06.002_bib0065","doi-asserted-by":"crossref","first-page":"1145","DOI":"10.1007\/s10544-009-9331-7","article-title":"Microfluidic compartmentalized co-culture platform for CNS axon myelination research","volume":"11","author":"Park","year":"2009","journal-title":"Biomed Microdevices"},{"key":"10.1016\/j.jneumeth.2013.06.002_bib0070","doi-asserted-by":"crossref","first-page":"599","DOI":"10.1038\/nmeth777","article-title":"A microfluidic culture platform for CNS axonal injury, regeneration and transport","volume":"2","author":"Taylor","year":"2005","journal-title":"Nat Methods"},{"key":"10.1016\/j.jneumeth.2013.06.002_bib0075","doi-asserted-by":"crossref","first-page":"185","DOI":"10.1615\/CritRevBiomedEng.v39.i3.20","article-title":"Microfluidic and compartmentalized platforms for neurobiological research","volume":"39","author":"Taylor","year":"2011","journal-title":"Crit Rev Biomed Eng"},{"key":"10.1016\/j.jneumeth.2013.06.002_bib0080","doi-asserted-by":"crossref","first-page":"37","DOI":"10.1146\/annurev.physiol.010908.163216","article-title":"Mechanisms of muscle degeneration, regeneration, and repair in the muscular dystrophies","volume":"71","author":"Wallace","year":"2009","journal-title":"Annu Rev Physiol"},{"key":"10.1016\/j.jneumeth.2013.06.002_bib0085","doi-asserted-by":"crossref","first-page":"31","DOI":"10.1038\/nprot.2009.193","article-title":"Isolation and enrichment of embryonic mouse motoneurons from the lumbar spinal cord of individual mouse embryos","volume":"5","author":"Wiese","year":"2010","journal-title":"Nat Protoc"},{"key":"10.1016\/j.jneumeth.2013.06.002_bib0090","doi-asserted-by":"crossref","first-page":"2284","DOI":"10.1093\/hmg\/ddq106","article-title":"Skeletal muscle-restricted expression of human SOD1 causes motor neuron degeneration in transgenic mice","volume":"19","author":"Wong","year":"2010","journal-title":"Hum Mol Genet"}],"container-title":["Journal of Neuroscience Methods"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0165027013002197?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0165027013002197?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,9,28]],"date-time":"2025-09-28T00:31:10Z","timestamp":1759019470000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0165027013002197"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2013,9]]},"references-count":18,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2013,9]]}},"alternative-id":["S0165027013002197"],"URL":"https:\/\/doi.org\/10.1016\/j.jneumeth.2013.06.002","relation":{},"ISSN":["0165-0270"],"issn-type":[{"value":"0165-0270","type":"print"}],"subject":[],"published":{"date-parts":[[2013,9]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Microfluidic primary culture model of the lower motor neuron\u2013neuromuscular junction circuit","name":"articletitle","label":"Article Title"},{"value":"Journal of Neuroscience Methods","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.jneumeth.2013.06.002","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"Copyright \u00a9 2013 Elsevier B.V. All rights reserved.","name":"copyright","label":"Copyright"}]}}