{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,26]],"date-time":"2026-02-26T15:51:36Z","timestamp":1772121096639,"version":"3.50.1"},"reference-count":30,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2020,2,1]],"date-time":"2020-02-01T00:00:00Z","timestamp":1580515200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2020,2,1]],"date-time":"2020-02-01T00:00:00Z","timestamp":1580515200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Journal of Neuroscience Methods"],"published-print":{"date-parts":[[2020,2]]},"DOI":"10.1016\/j.jneumeth.2019.108546","type":"journal-article","created":{"date-parts":[[2019,12,9]],"date-time":"2019-12-09T00:31:46Z","timestamp":1575851506000},"page":"108546","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":0,"special_numbering":"C","title":["Long-term culturing of porcine nodose ganglia"],"prefix":"10.1016","volume":"332","author":[{"given":"Shin-Ping","family":"Kuan","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kalina R.","family":"Atanasova","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maria V.","family":"Guevara","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Emily N.","family":"Collins","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4074-9070","authenticated-orcid":false,"given":"Leah R.","family":"Reznikov","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1016\/j.jneumeth.2019.108546_bib0005","first-page":"101","article-title":"Antibiotic supplements affect electrophysiological properties and excitability of rat hippocampal pyramidal neurons in primary culture","volume":"17","author":"Bahrami","year":"2013","journal-title":"Iran. Biomed. J."},{"key":"10.1016\/j.jneumeth.2019.108546_bib0010","doi-asserted-by":"crossref","first-page":"674","DOI":"10.1002\/jnr.490420510","article-title":"Serum-free B27\/neurobasal medium supports differentiated growth of neurons from the striatum, substantia nigra, septum, cerebral cortex, cerebellum, and dentate gyrus","volume":"42","author":"Brewer","year":"1995","journal-title":"J. Neurosci. Res."},{"key":"10.1016\/j.jneumeth.2019.108546_bib0015","doi-asserted-by":"crossref","first-page":"622","DOI":"10.1016\/j.cell.2015.03.022","article-title":"Vagal sensory neuron subtypes that differentially control breathing","volume":"161","author":"Chang","year":"2015","journal-title":"Cell"},{"key":"10.1016\/j.jneumeth.2019.108546_bib0020","doi-asserted-by":"crossref","first-page":"245","DOI":"10.1146\/annurev-animal-022114-111049","article-title":"The domestic piglet: an important model for investigating the neurodevelopmental consequences of early life insults","volume":"3","author":"Conrad","year":"2015","journal-title":"Annu. Rev. Anim. Biosci."},{"key":"10.1016\/j.jneumeth.2019.108546_bib0025","doi-asserted-by":"crossref","first-page":"234","DOI":"10.1016\/j.jneumeth.2014.07.009","article-title":"An organotypic brain slice preparation from adult patients with temporal lobe epilepsy","volume":"235","author":"Eugene","year":"2014","journal-title":"J. Neurosci. Methods"},{"key":"10.1016\/j.jneumeth.2019.108546_bib0030","doi-asserted-by":"crossref","first-page":"67","DOI":"10.1016\/j.jgg.2012.07.014","article-title":"Genetically modified pig models for human diseases","volume":"40","author":"Fan","year":"2013","journal-title":"J. Genet. Genomics"},{"key":"10.1016\/j.jneumeth.2019.108546_bib0035","doi-asserted-by":"crossref","first-page":"2541","DOI":"10.1038\/s41467-019-10493-6","article-title":"Tumor-associated reactive astrocytes aid the evolution of immunosuppressive environment in glioblastoma","volume":"10","author":"Henrik Heiland","year":"2019","journal-title":"Nat. Commun."},{"key":"10.1016\/j.jneumeth.2019.108546_bib0040","doi-asserted-by":"crossref","first-page":"41","DOI":"10.1016\/0006-8993(82)90609-6","article-title":"Membrane depolarization and prolongation of calcium-dependent action potentials of mouse neurons in cell culture by two convulsants: bicuculline and penicillin","volume":"232","author":"Heyer","year":"1982","journal-title":"Brain Res."},{"key":"10.1016\/j.jneumeth.2019.108546_bib0045","doi-asserted-by":"crossref","first-page":"86","DOI":"10.1016\/j.neuroscience.2015.07.086","article-title":"Organotypic brain slice cultures: a review","volume":"305","author":"Humpel","year":"2015","journal-title":"Neuroscience"},{"key":"10.1016\/j.jneumeth.2019.108546_bib0050","doi-asserted-by":"crossref","first-page":"1985","DOI":"10.1164\/ajrccm.161.6.9908051","article-title":"Nerve growth factor-induced phenotypic switch in guinea pig airway sensory neurons","volume":"161","author":"Hunter","year":"2000","journal-title":"Am. J. Respir. Crit. Care Med."},{"key":"10.1016\/j.jneumeth.2019.108546_bib0055","doi-asserted-by":"crossref","first-page":"8160","DOI":"10.1074\/jbc.M805632200","article-title":"Different transcription factors regulate nestin gene expression during P19 cell neural differentiation and central nervous system development","volume":"284","author":"Jin","year":"2009","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.jneumeth.2019.108546_bib0060","doi-asserted-by":"crossref","first-page":"1852","DOI":"10.1073\/pnas.95.4.1852","article-title":"Dehydroepiandrosterone (DHEA) and DHEA-sulfate (DHEAS) protect hippocampal neurons against excitatory amino acid-induced neurotoxicity","volume":"95","author":"Kimonides","year":"1998","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"key":"10.1016\/j.jneumeth.2019.108546_bib0065","doi-asserted-by":"crossref","first-page":"739","DOI":"10.1586\/14737175.2016.1166053","article-title":"Developments in 3D neural cell culture models: the future of neurotherapeutics testing?","volume":"16","author":"Ko","year":"2016","journal-title":"Expert Rev. Neurother."},{"key":"10.1016\/j.jneumeth.2019.108546_bib0070","doi-asserted-by":"crossref","first-page":"8","DOI":"10.1186\/2047-1440-1-8","article-title":"The pig as a model for translational research: overview of porcine animal models at Jichi Medical University","volume":"1","author":"Kobayashi","year":"2012","journal-title":"Transplant. Res."},{"key":"10.1016\/j.jneumeth.2019.108546_bib0075","doi-asserted-by":"crossref","first-page":"728","DOI":"10.1016\/j.neubiorev.2007.02.003","article-title":"The use of pigs in neuroscience: modeling brain disorders","volume":"31","author":"Lind","year":"2007","journal-title":"Neurosci. Biobehav. Rev."},{"key":"10.1016\/j.jneumeth.2019.108546_bib0080","doi-asserted-by":"crossref","first-page":"13","DOI":"10.1093\/ilar\/ily025","article-title":"Fundamental concepts for semiquantitative tissue scoring in translational research","volume":"59","author":"Meyerholz","year":"2018","journal-title":"ILAR J."},{"key":"10.1016\/j.jneumeth.2019.108546_bib0085","doi-asserted-by":"crossref","first-page":"93","DOI":"10.1016\/j.jneumeth.2017.06.005","article-title":"Simple and reproducible approaches for the collection of select porcine ganglia","volume":"289","author":"Meyerholz","year":"2017","journal-title":"J. Neurosci. Methods"},{"key":"10.1016\/j.jneumeth.2019.108546_bib0090","first-page":"501","article-title":"Over a century of neuron culture: from the hanging drop to microfluidic devices","volume":"85","author":"Millet","year":"2012","journal-title":"Yale J. Biol. Med."},{"key":"10.1016\/j.jneumeth.2019.108546_bib0095","doi-asserted-by":"crossref","DOI":"10.26508\/lsa.201900305","article-title":"Human organotypic brain slice culture: a novel framework for environmental research in neuro-oncology","volume":"2","author":"Ravi","year":"2019","journal-title":"Life Sci. Alliance"},{"key":"10.1016\/j.jneumeth.2019.108546_bib0100","doi-asserted-by":"crossref","first-page":"3602","DOI":"10.1073\/pnas.90.8.3602","article-title":"Proliferation, differentiation, and long-term culture of primary hippocampal neurons","volume":"90","author":"Ray","year":"1993","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"key":"10.1016\/j.jneumeth.2019.108546_bib0105","doi-asserted-by":"crossref","first-page":"1147","DOI":"10.1016\/j.chest.2016.11.009","article-title":"Cystic fibrosis and the nervous system","volume":"151","author":"Reznikov","year":"2017","journal-title":"Chest"},{"key":"10.1016\/j.jneumeth.2019.108546_bib0110","doi-asserted-by":"crossref","first-page":"3083","DOI":"10.1073\/pnas.1222729110","article-title":"CFTR-deficient pigs display peripheral nervous system defects at birth","volume":"110","author":"Reznikov","year":"2013","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"key":"10.1016\/j.jneumeth.2019.108546_bib0115","doi-asserted-by":"crossref","first-page":"219","DOI":"10.1007\/s00408-018-0091-0","article-title":"Solitary cholinergic stimulation induces airway hyperreactivity and transcription of distinct pro-inflammatory pathways","volume":"196","author":"Reznikov","year":"2018","journal-title":"Lung"},{"key":"10.1016\/j.jneumeth.2019.108546_bib0120","doi-asserted-by":"crossref","first-page":"L131","DOI":"10.1152\/ajplung.00417.2018","article-title":"Sex-specific airway hyperreactivity and sex-specific transcriptome remodeling in neonatal piglets challenged with intra-airway acid","volume":"316","author":"Reznikov","year":"2019","journal-title":"Am. J. Physiol. Lung Cell Mol. Physiol."},{"key":"10.1016\/j.jneumeth.2019.108546_bib0125","doi-asserted-by":"crossref","first-page":"L240","DOI":"10.1152\/ajplung.90203.2008","article-title":"The porcine lung as a potential model for cystic fibrosis","volume":"295","author":"Rogers","year":"2008","journal-title":"Am. J. Physiol. Lung Cell Mol. Physiol."},{"key":"10.1016\/j.jneumeth.2019.108546_bib0130","doi-asserted-by":"crossref","first-page":"73","DOI":"10.1007\/s10616-009-9234-8","article-title":"Neuron survival in vitro is more influenced by the developmental age of the cells than by glucose condition","volume":"61","author":"Sepehr","year":"2009","journal-title":"Cytotechnology"},{"key":"10.1016\/j.jneumeth.2019.108546_bib0135","doi-asserted-by":"crossref","first-page":"647","DOI":"10.1038\/nrm3873","article-title":"Three-dimensional organotypic culture: experimental models of mammalian biology and disease","volume":"15","author":"Shamir","year":"2014","journal-title":"Nat. Rev. Mol. Cell Biol."},{"key":"10.1016\/j.jneumeth.2019.108546_bib0140","doi-asserted-by":"crossref","first-page":"173","DOI":"10.1016\/0165-0270(91)90128-M","article-title":"A simple method for organotypic cultures of nervous tissue","volume":"37","author":"Stoppini","year":"1991","journal-title":"J. Neurosci. Methods"},{"key":"10.1016\/j.jneumeth.2019.108546_bib0145","doi-asserted-by":"crossref","first-page":"335","DOI":"10.1097\/NEN.0b013e3181d3cb52","article-title":"Alexander disease mutant glial fibrillary acidic protein compromises glutamate transport in astrocytes","volume":"69","author":"Tian","year":"2010","journal-title":"J. Neuropathol. Exp. Neurol."},{"key":"10.1016\/j.jneumeth.2019.108546_bib0150","first-page":"1","article-title":"Isolation, culture and long-term maintenance of primary mesencephalic dopaminergic neurons from embryonic rodent brains","volume":"96","author":"Weinert","year":"2015","journal-title":"J. Vis. Exp."}],"container-title":["Journal of Neuroscience Methods"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0165027019304030?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0165027019304030?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,9,28]],"date-time":"2025-09-28T00:51:07Z","timestamp":1759020667000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0165027019304030"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,2]]},"references-count":30,"alternative-id":["S0165027019304030"],"URL":"https:\/\/doi.org\/10.1016\/j.jneumeth.2019.108546","relation":{"has-preprint":[{"id-type":"doi","id":"10.1101\/706945","asserted-by":"object"}]},"ISSN":["0165-0270"],"issn-type":[{"value":"0165-0270","type":"print"}],"subject":[],"published":{"date-parts":[[2020,2]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Long-term culturing of porcine nodose ganglia","name":"articletitle","label":"Article Title"},{"value":"Journal of Neuroscience Methods","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.jneumeth.2019.108546","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2019 Elsevier B.V. All rights reserved.","name":"copyright","label":"Copyright"}],"article-number":"108546"}}