{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,1]],"date-time":"2026-06-01T21:52:35Z","timestamp":1780350755774,"version":"3.54.1"},"reference-count":76,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2024,5,1]],"date-time":"2024-05-01T00:00:00Z","timestamp":1714521600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2024,5,1]],"date-time":"2024-05-01T00:00:00Z","timestamp":1714521600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2024,5,1]],"date-time":"2024-05-01T00:00:00Z","timestamp":1714521600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-017"},{"start":{"date-parts":[[2024,5,1]],"date-time":"2024-05-01T00:00:00Z","timestamp":1714521600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2024,5,1]],"date-time":"2024-05-01T00:00:00Z","timestamp":1714521600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-012"},{"start":{"date-parts":[[2024,5,1]],"date-time":"2024-05-01T00:00:00Z","timestamp":1714521600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2024,5,1]],"date-time":"2024-05-01T00:00:00Z","timestamp":1714521600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-004"}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Journal of Neuroscience Methods"],"published-print":{"date-parts":[[2024,5]]},"DOI":"10.1016\/j.jneumeth.2024.110085","type":"journal-article","created":{"date-parts":[[2024,2,20]],"date-time":"2024-02-20T16:15:00Z","timestamp":1708445700000},"page":"110085","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":3,"special_numbering":"C","title":["Systematic development of immunohistochemistry protocol for large cryosections-specific to non-perfused fetal brain"],"prefix":"10.1016","volume":"405","author":[{"given":"Karthika","family":"Pandurangan","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jaikishan","family":"Jayakumar","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stephen","family":"Savoia","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Reetuparna","family":"Nanda","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S.","family":"Lata","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"E. Harish","family":"Kumar","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Suresh","family":"S.","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sudha","family":"Vasudevan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chitra","family":"Srinivasan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jayaraj","family":"Joseph","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohanasankar","family":"Sivaprakasam","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Richa","family":"Verma","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.jneumeth.2024.110085_bib1","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0003378","article-title":"A new antigen retrieval technique for human brain tissue","volume":"3","author":"Alel\u00fa-Paz","year":"2008","journal-title":"PLoS ONE"},{"key":"10.1016\/j.jneumeth.2024.110085_bib2","doi-asserted-by":"crossref","first-page":"631","DOI":"10.1093\/cercor\/bht254","article-title":"The Early Fetal Development of Human Neocortical GABAergic Interneurons","volume":"25","author":"Al-Jaberi","year":"2015","journal-title":"Cereb. Cortex"},{"key":"10.1016\/j.jneumeth.2024.110085_bib3","doi-asserted-by":"crossref","first-page":"141","DOI":"10.1186\/s40478-020-01015-3","article-title":"Assessment of radial glia in the frontal lobe of fetuses with Down syndrome","volume":"8","author":"Baburamani","year":"2020","journal-title":"Acta Neuropathol. Commun."},{"key":"10.1016\/j.jneumeth.2024.110085_bib4","series-title":"The Human Brain During the Second Trimester","author":"Bayer","year":"2005"},{"key":"10.1016\/j.jneumeth.2024.110085_bib5","doi-asserted-by":"crossref","first-page":"155","DOI":"10.1016\/j.neuroscience.2013.08.048","article-title":"Immunohistochemical localization of oxytocin receptors in human brain","volume":"253","author":"Boccia","year":"2013","journal-title":"Neuroscience"},{"key":"10.1016\/j.jneumeth.2024.110085_bib6","doi-asserted-by":"crossref","first-page":"283","DOI":"10.1097\/01.0000146524.74402.a4","article-title":"Effect of Heat-Induced Antigen Retrieval Following Inconsistent Formalin Fixation","volume":"13","author":"Boenisch","year":"2005","journal-title":"Appl. Immunohistochem. Mol. Morphol."},{"key":"10.1016\/j.jneumeth.2024.110085_bib7","series-title":"in: Immunohistochemistry: Basics and Methods","first-page":"47","article-title":"Immunostaining Enhancement","author":"Buchwalow","year":"2010"},{"key":"10.1016\/j.jneumeth.2024.110085_bib8","first-page":"936","article-title":"Comparison of indirect peroxidase and avidin-biotin-peroxidase complex (ABC) immunohistochemical staining procedures for c-fos in rat brain","volume":"234","author":"Butler","year":"2019","journal-title":"joa 12967"},{"key":"10.1016\/j.jneumeth.2024.110085_bib9","doi-asserted-by":"crossref","first-page":"1593","DOI":"10.1002\/hbm.26160","article-title":"Detailed anatomic segmentations of a fetal brain diffusion tensor imaging atlas between 23 and 30 weeks of gestation","volume":"44","author":"Calixto","year":"2023","journal-title":"Hum. Brain Mapp."},{"key":"10.1016\/j.jneumeth.2024.110085_bib10","doi-asserted-by":"crossref","first-page":"562","DOI":"10.1159\/000231250","article-title":"Effect of Formaldehyde, Glutaraldehyde and Sucrose on the Tissue Antigenicity","volume":"47","author":"Deng","year":"2009","journal-title":"Int. Arch. Allergy Appl. Immunol."},{"key":"10.1016\/j.jneumeth.2024.110085_bib11","doi-asserted-by":"crossref","first-page":"6","DOI":"10.1002\/cne.25243","article-title":"Cellular resolution anatomical and molecular atlases for prenatal human brains","volume":"530","author":"Ding","year":"2022","journal-title":"J. Comp. Neurol."},{"key":"10.1016\/j.jneumeth.2024.110085_bib12","doi-asserted-by":"crossref","first-page":"911","DOI":"10.1016\/j.neuroimage.2012.01.024","article-title":"Brain templates and atlases","volume":"62","author":"Evans","year":"2012","journal-title":"NeuroImage, 20 YEARS fMRI"},{"key":"10.1016\/j.jneumeth.2024.110085_bib13","doi-asserted-by":"crossref","first-page":"1555","DOI":"10.1177\/42.12.7983356","article-title":"Microwave-stimulated antigen retrieval is pH and temperature dependent","volume":"42","author":"Evers","year":"1994","journal-title":"J. Histochem Cytochem"},{"key":"10.1016\/j.jneumeth.2024.110085_bib14","doi-asserted-by":"crossref","first-page":"197","DOI":"10.1016\/S0165-0270(96)02204-2","article-title":"An optimal antigen retrieval method suitable for different antibodies on human brain tissue stored for several years in formaldehyde fixative","volume":"72","author":"Evers","year":"1997","journal-title":"J. Neurosci. Methods"},{"key":"10.1016\/j.jneumeth.2024.110085_bib15","first-page":"615","article-title":"[Antigen retrieval: its significance and drawbacks in immunohistochemistry]","volume":"71","author":"Ezaki","year":"1996","journal-title":"Kaibogaku Zasshi"},{"key":"10.1016\/j.jneumeth.2024.110085_bib16","doi-asserted-by":"crossref","first-page":"6","DOI":"10.24171\/j.phrp.2019.10.1.03","article-title":"Natural Infection with Rabies Virus: A Histopathological and Immunohistochemical Study of Human Brains","volume":"10","author":"Farahtaj","year":"2019","journal-title":"Osong Public Health Res Perspect."},{"key":"10.1016\/j.jneumeth.2024.110085_bib17","doi-asserted-by":"crossref","first-page":"2196","DOI":"10.1093\/cercor\/bhp009","article-title":"Selective Depletion of Molecularly Defined Cortical Interneurons in Human Holoprosencephaly with Severe Striatal Hypoplasia","volume":"19","author":"Fertuzinhos","year":"2009","journal-title":"Cereb. Cortex"},{"key":"10.1016\/j.jneumeth.2024.110085_bib18","doi-asserted-by":"crossref","first-page":"845","DOI":"10.1177\/33.8.3894502","article-title":"Formaldehyde fixation","volume":"33","author":"Fox","year":"1985","journal-title":"J. Histochem Cytochem"},{"key":"10.1016\/j.jneumeth.2024.110085_bib19","doi-asserted-by":"crossref","first-page":"236","DOI":"10.1097\/00129039-200009000-00011","article-title":"Methods of Antigen Recovery Vary in Their Usefulness in Unmasking Specific Antigens in Immunohistochemistry","volume":"8","author":"Frost","year":"2000","journal-title":"Appl. Immunohistochem. Mol. Morphol."},{"key":"10.1016\/j.jneumeth.2024.110085_bib20","doi-asserted-by":"crossref","first-page":"476","DOI":"10.1038\/s41598-017-00525-w","article-title":"A normative spatiotemporal MRI atlas of the fetal brain for automatic segmentation and analysis of early brain growth","volume":"7","author":"Gholipour","year":"2017","journal-title":"Sci. Rep."},{"key":"10.1016\/j.jneumeth.2024.110085_bib21","doi-asserted-by":"crossref","DOI":"10.3389\/fnana.2015.00022","article-title":"Development of a highly sensitive immunohistochemical method to detect neurochemical molecules in formalin-fixed and paraffin-embedded tissues from autopsied human brains","volume":"9","author":"Goto","year":"2015","journal-title":"Front. Neuroanat."},{"key":"10.1016\/j.jneumeth.2024.110085_bib22","doi-asserted-by":"crossref","first-page":"93","DOI":"10.1007\/s00418-015-1316-4","article-title":"Factors affecting immunoreactivity in long-term storage of formalin-fixed paraffin-embedded tissue sections","volume":"144","author":"Grillo","year":"2015","journal-title":"Histochem Cell Biol."},{"key":"10.1016\/j.jneumeth.2024.110085_bib23","doi-asserted-by":"crossref","first-page":"863","DOI":"10.1007\/s00381-015-2713-7","article-title":"Expression pattern of NeuN and GFAP during human fetal spinal cord development","volume":"31","author":"Guo","year":"2015","journal-title":"Childs Nerv. Syst."},{"key":"10.1016\/j.jneumeth.2024.110085_bib24","doi-asserted-by":"crossref","first-page":"930","DOI":"10.1093\/jnen\/nlz073","article-title":"Tau phosphorylation and aggregation in the developing human brain","volume":"78","author":"Hefti","year":"2019","journal-title":"J. Neuropathol. Exp. Neurol."},{"key":"10.1016\/j.jneumeth.2024.110085_bib25","doi-asserted-by":"crossref","first-page":"590","DOI":"10.1111\/joa.12948","article-title":"Astrogliogenesis in human fetal brain: complex spatiotemporal immunoreactivity patterns of GFAP, S100, AQP 4 and YKL -40","volume":"235","author":"Holst","year":"2019","journal-title":"J. Anat."},{"key":"10.1016\/j.jneumeth.2024.110085_bib26","doi-asserted-by":"crossref","first-page":"97","DOI":"10.1023\/A:1020954611464","article-title":"The Enhanced Reactivity of Endogenous Biotin-like Molecules by Antigen Retrieval Procedures and Signal Amplification with Tyramine","volume":"34","author":"Hyung Kim","year":"2002","journal-title":"Histochem J."},{"key":"10.1016\/j.jneumeth.2024.110085_bib27","doi-asserted-by":"crossref","first-page":"2440","DOI":"10.1002\/cne.24721","article-title":"The brain of the tree pangolin ( Manis tricuspis). VI. The brainstem and cerebellum","volume":"527","author":"Imam","year":"2019","journal-title":"J. Comp. Neurol."},{"key":"10.1016\/j.jneumeth.2024.110085_bib28","doi-asserted-by":"crossref","first-page":"149","DOI":"10.1016\/S0165-0270(99)00142-9","article-title":"A simple and sensitive antigen retrieval method for free-floating and slide-mounted tissue sections","volume":"93","author":"Jiao","year":"1999","journal-title":"J. Neurosci. Methods"},{"key":"10.1016\/j.jneumeth.2024.110085_bib29","doi-asserted-by":"crossref","DOI":"10.1016\/j.heliyon.2023.e20626","article-title":"Triple enzymatic immunochemistry for interneuron populations in postmortem human cerebral cortex","volume":"9","author":"Juarez","year":"2023","journal-title":"Heliyon"},{"key":"10.1016\/j.jneumeth.2024.110085_bib30","doi-asserted-by":"crossref","first-page":"119","DOI":"10.1080\/10520290310001593775","article-title":"A comparison of microwave heating and proteolytic pretreatment antigen retrieval techniques in formalin fixed, paraffin embedded tissues","volume":"78","author":"Kahveci","year":"2003","journal-title":"Biotech. Histochem."},{"key":"10.1016\/j.jneumeth.2024.110085_bib31","doi-asserted-by":"crossref","first-page":"30395","DOI":"10.1074\/jbc.M109.045567","article-title":"Peroxidase Activity of Hemoglobin\u00b7Haptoglobin Complexes: Covalent Aggregation and oxidative stress in plasma and macrophages*","volume":"284","author":"Kapralov","year":"2009","journal-title":"Journal of Biological Chemistry"},{"key":"10.1016\/j.jneumeth.2024.110085_bib32","doi-asserted-by":"crossref","first-page":"424","DOI":"10.1002\/mrm.1910300405","article-title":"Development of the human brain: In vivo quantification of metabolite and water content with proton magnetic resonance spectroscopy","volume":"30","author":"Kreis","year":"1993","journal-title":"Magn. Reson. Med."},{"key":"10.1016\/j.jneumeth.2024.110085_bib33","doi-asserted-by":"crossref","first-page":"81","DOI":"10.1016\/j.proghi.2009.11.001","article-title":"Detection of endogenous and immuno-bound peroxidase \u2014 The status Quo in histochemistry","volume":"45","author":"Krieg","year":"2010","journal-title":"Prog. Histochem. Cytochem."},{"key":"10.1016\/j.jneumeth.2024.110085_bib34","doi-asserted-by":"crossref","DOI":"10.3389\/fnana.2023.1292655","article-title":"A technology platform for standardized cryoprotection and freezing of large-volume brain tissues for high-resolution histology","volume":"17","author":"Kumarasami","year":"2023","journal-title":"Front. Neuroanat."},{"key":"10.1016\/j.jneumeth.2024.110085_bib35","doi-asserted-by":"crossref","first-page":"429","DOI":"10.1016\/S0002-9440(10)65586-1","article-title":"Expression of Matrix Metalloproteinases and Their Tissue Inhibitors in Human Brain Tumors","volume":"153","author":"Lampert","year":"1998","journal-title":"Am. J. Pathol."},{"key":"10.1016\/j.jneumeth.2024.110085_bib36","doi-asserted-by":"crossref","first-page":"129","DOI":"10.1097\/PAP.0b013e31803250c7","article-title":"How does antigen retrieval work?","volume":"14","author":"Leong","year":"2007","journal-title":"Adv. Anat. Pathol."},{"key":"10.1016\/j.jneumeth.2024.110085_bib37","doi-asserted-by":"crossref","first-page":"815","DOI":"10.1002\/cne.901930316","article-title":"Immunoperoxidase localization of glial fibrillary acidic protein in radial glial cells and astrocytes of the developing rhesus monkey brain","volume":"193","author":"Levitt","year":"1980","journal-title":"J. Comp. Neurol."},{"key":"10.1016\/j.jneumeth.2024.110085_bib38","doi-asserted-by":"crossref","first-page":"91","DOI":"10.1007\/s00418-018-1742-1","article-title":"Characteristics of the tissue section that influence the staining outcome in immunohistochemistry","volume":"151","author":"Libard","year":"2019","journal-title":"Histochem Cell Biol."},{"key":"10.1016\/j.jneumeth.2024.110085_bib39","doi-asserted-by":"crossref","DOI":"10.7554\/eLife.40042","article-title":"A high-throughput neurohistological pipeline for brain-wide mesoscale connectivity mapping of the common marmoset","volume":"8","author":"Lin","year":"2019","journal-title":"eLife"},{"key":"10.1016\/j.jneumeth.2024.110085_bib40","doi-asserted-by":"crossref","first-page":"295","DOI":"10.1002\/jnr.20354","article-title":"Characterization of the neuronal marker NeuN as a multiply phosphorylated antigen with discrete subcellular localization","volume":"79","author":"Lind","year":"2005","journal-title":"J. Neurosci. Res."},{"key":"10.1016\/j.jneumeth.2024.110085_bib41","doi-asserted-by":"crossref","first-page":"201","DOI":"10.1369\/jhc.7A7187.2007","article-title":"Immunohistochemical Markers for Quantitative Studies of Neurons and Glia in Human Neocortex","volume":"56","author":"Lyck","year":"2008","journal-title":"J. Histochem Cytochem"},{"key":"10.1016\/j.jneumeth.2024.110085_bib42","doi-asserted-by":"crossref","DOI":"10.1016\/j.isci.2023.106608","article-title":"Parvalbumin as a neurochemical marker of the primate optic radiation","volume":"26","author":"Ma","year":"2023","journal-title":"iScience"},{"key":"10.1016\/j.jneumeth.2024.110085_bib43","series-title":"Biobanking, Methods in Molecular Biology","doi-asserted-by":"crossref","first-page":"289","DOI":"10.1007\/978-1-4939-8935-5_25","article-title":"An introduction to the performance of immunohistochemistry","author":"Magaki","year":"2019"},{"key":"10.1016\/j.jneumeth.2024.110085_bib44","doi-asserted-by":"crossref","first-page":"176","DOI":"10.1007\/BF00688206","article-title":"Histopathological study of alobar holoprosencephaly","volume":"78","author":"Mizuguchi","year":"1989","journal-title":"Acta Neuropathol."},{"key":"10.1016\/j.jneumeth.2024.110085_bib45","doi-asserted-by":"crossref","first-page":"31","DOI":"10.3960\/jslrt.17017","article-title":"Optimum immunohistochemical procedures for analysis of macrophages in human and mouse formalin fixed paraffin-embedded tissue samples","volume":"57","author":"Nakagawa","year":"2017","journal-title":"JCEH"},{"key":"10.1016\/j.jneumeth.2024.110085_bib46","doi-asserted-by":"crossref","first-page":"2075","DOI":"10.1523\/JNEUROSCI.11-07-02075.1991","article-title":"Immunohistochemical localization of an inositol 1,4,5-trisphosphate receptor, P400, in neural tissue: studies in developing and adult mouse brain","volume":"11","author":"Nakanishi","year":"1991","journal-title":"J. Neurosci."},{"key":"10.1016\/j.jneumeth.2024.110085_bib47","doi-asserted-by":"crossref","first-page":"214","DOI":"10.1016\/S1385-299X(02)00148-4","article-title":"A simplified method for combined immunohistochemistry and in-situ hybridization in fresh-frozen, cryocut mouse brain sections","volume":"9","author":"Newton","year":"2002","journal-title":"Brain Res. Protoc."},{"key":"10.1016\/j.jneumeth.2024.110085_bib48","doi-asserted-by":"crossref","first-page":"936","DOI":"10.1016\/j.ajhg.2019.03.010","article-title":"Homozygous Mutations in CSF1R Cause a Pediatric-Onset Leukoencephalopathy and Can Result in Congenital Absence of Microglia","volume":"104","author":"Oosterhof","year":"2019","journal-title":"Am. J. Hum. Genet."},{"key":"10.1016\/j.jneumeth.2024.110085_bib49","doi-asserted-by":"crossref","first-page":"1323","DOI":"10.1016\/0006-2952(90)90399-6","article-title":"Surfactant-induced cell toxicity and cell lysis","volume":"40","author":"Partearroyo","year":"1990","journal-title":"Biochem. Pharmacol."},{"key":"10.1016\/j.jneumeth.2024.110085_bib50","doi-asserted-by":"crossref","first-page":"313","DOI":"10.1016\/0306-4522(85)90215-5","article-title":"Immunohistochemical localization of macrophages and microglia in the adult and developing mouse brain","volume":"15","author":"Perry","year":"1985","journal-title":"Neuroscience"},{"key":"10.1016\/j.jneumeth.2024.110085_bib51","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0102363","article-title":"High-Throughput Method of Whole-Brain Sectioning, Using the Tape-Transfer Technique","volume":"10","author":"Pinskiy","year":"2015","journal-title":"PLoS ONE"},{"key":"10.1016\/j.jneumeth.2024.110085_bib52","first-page":"24","volume":"5","author":"Prabhu","year":"2018","journal-title":"Vet. Sci."},{"key":"10.1016\/j.jneumeth.2024.110085_bib53","doi-asserted-by":"crossref","first-page":"81","DOI":"10.1016\/S0165-0270(97)00050-2","article-title":"The shrinkage of the human brain stem during formalin fixation and embedding in paraffin","volume":"75","author":"Quester","year":"1997","journal-title":"J. Neurosci. Methods"},{"key":"10.1016\/j.jneumeth.2024.110085_bib54","series-title":"Drug Safety Evaluation, Methods in Molecular Biology","doi-asserted-by":"crossref","first-page":"83","DOI":"10.1007\/978-1-60761-849-2_5","article-title":"Principles and Methods of Immunohistochemistry","author":"Ramos-Vara","year":"2011"},{"key":"10.1016\/j.jneumeth.2024.110085_bib55","doi-asserted-by":"crossref","first-page":"50","DOI":"10.1159\/000071021","article-title":"Distribution and Morphology of GFAP-Positive Astrocytes in the Human Fetal Brain at Second Trimester","volume":"2","author":"Rezaie","year":"2003","journal-title":"Neuroembryol Aging"},{"key":"10.1016\/j.jneumeth.2024.110085_bib56","doi-asserted-by":"crossref","DOI":"10.1016\/j.jneumeth.2021.109354","article-title":"Virtual histology of an entire mouse brain from formalin fixation to paraffin embedding. Part 1: Data acquisition, anatomical feature segmentation, tracking global volume and density changes","volume":"364","author":"Rodgers","year":"2021","journal-title":"J. Neurosci. Methods"},{"key":"10.1016\/j.jneumeth.2024.110085_bib57","doi-asserted-by":"crossref","first-page":"308","DOI":"10.1007\/BF00686089","article-title":"Astrocytes in the developing human brain: An immunohistochemical study","volume":"70","author":"Roessmann","year":"1986","journal-title":"Acta Neuropathol."},{"key":"10.1016\/j.jneumeth.2024.110085_bib58","doi-asserted-by":"crossref","first-page":"41","DOI":"10.1016\/S0387-7604(98)00068-0","article-title":"Synaptophysin immunocytochemistry with thermal intensification: a marker of terminal axonal maturation in the human fetal nervous system","volume":"21","author":"Sarnat","year":"1999","journal-title":"Brain Dev."},{"key":"10.1016\/j.jneumeth.2024.110085_bib59","doi-asserted-by":"crossref","first-page":"88","DOI":"10.1016\/S0387-7604(97)00111-3","article-title":"Neuronal nuclear antigen (NeuN): a marker of neuronal maturation in the early human fetal nervous system","volume":"20","author":"Sarnat","year":"1998","journal-title":"Brain Dev."},{"key":"10.1016\/j.jneumeth.2024.110085_bib60","doi-asserted-by":"crossref","first-page":"128","DOI":"10.1007\/BF00688096","article-title":"Immunohistochemical study of the early human fetal brain","volume":"76","author":"Sasaki","year":"1988","journal-title":"Acta Neuropathol."},{"key":"10.1016\/j.jneumeth.2024.110085_bib61","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1038\/jid.2014.541","article-title":"Basics of Immunohistochemistry","volume":"135","author":"Schacht","year":"2015","journal-title":"J. Invest. Dermatol."},{"key":"10.1016\/j.jneumeth.2024.110085_bib62","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1002\/(SICI)1097-0029(19990701)46:1<1::AID-JEMT1>3.0.CO;2-P","article-title":"Differential expression of calbindin and calretinin in the human fetal amygdala","volume":"46","author":"Setzer","year":"1999","journal-title":"Microsc. Res. Tech."},{"key":"10.1016\/j.jneumeth.2024.110085_bib63","doi-asserted-by":"crossref","DOI":"10.1038\/s41586-023-06569-5","article-title":"Spatial atlas of the mouse central nervous system at molecular resolution","author":"Shi","year":"2023","journal-title":"Nature"},{"key":"10.1016\/j.jneumeth.2024.110085_bib64","doi-asserted-by":"crossref","first-page":"1599","DOI":"10.1177\/41.11.7691930","article-title":"Antigen retrieval technique utilizing citrate buffer or urea solution for immunohistochemical demonstration of androgen receptor in formalin-fixed paraffin sections","volume":"41","author":"Shi","year":"1993","journal-title":"J. Histochem Cytochem"},{"key":"10.1016\/j.jneumeth.2024.110085_bib65","doi-asserted-by":"crossref","first-page":"193","DOI":"10.1177\/43.2.7822775","article-title":"Antigen retrieval immunohistochemistry under the influence of pH using monoclonal antibodies","volume":"43","author":"Shi","year":"1995","journal-title":"J. Histochem Cytochem"},{"key":"10.1016\/j.jneumeth.2024.110085_bib66","doi-asserted-by":"crossref","first-page":"109","DOI":"10.1016\/S1385-299X(97)00029-9","article-title":"Immunocytochemistry of formalin-fixed human brain tissues: microwave irradiation of free-floating sections","volume":"2","author":"Shiurba","year":"1998","journal-title":"Brain Res. Protoc."},{"key":"10.1016\/j.jneumeth.2024.110085_bib67","doi-asserted-by":"crossref","first-page":"17","DOI":"10.1007\/BF00321896","article-title":"The developing neuroepithelium in human embryonic and fetal brain studied with vimentin-immunocytochemistry","volume":"180","author":"Stagaard","year":"1989","journal-title":"Anat. Embryol."},{"key":"10.1016\/j.jneumeth.2024.110085_bib68","doi-asserted-by":"crossref","first-page":"D996","DOI":"10.1093\/nar\/gks1042","article-title":"Allen Brain Atlas: an integrated spatio-temporal portal for exploring the central nervous system","volume":"41","author":"Sunkin","year":"2013","journal-title":"Nucleic Acids Res."},{"key":"10.1016\/j.jneumeth.2024.110085_bib69","doi-asserted-by":"crossref","DOI":"10.3389\/fnint.2022.760887","article-title":"Energy supply per neuron is constrained by capillary density in the mouse brain","volume":"16","author":"Ventura-Antunes","year":"2022","journal-title":"Front. Integr. Neurosci."},{"key":"10.1016\/j.jneumeth.2024.110085_bib70","doi-asserted-by":"crossref","DOI":"10.4081\/ejh.2016.2563","article-title":"Immunohistochemical markers of neural progenitor cells in the early embryonic human cerebral cortex","volume":"60","author":"Vinci","year":"2016","journal-title":"Eur. J. Histochem"},{"key":"10.1016\/j.jneumeth.2024.110085_bib71","doi-asserted-by":"crossref","first-page":"2719","DOI":"10.1038\/nprot.2006.354","article-title":"Immunohistochemical staining of post-mortem adult human brain sections","volume":"1","author":"Waldvogel","year":"2006","journal-title":"Nat. Protoc."},{"key":"10.1016\/j.jneumeth.2024.110085_bib72","doi-asserted-by":"crossref","first-page":"193","DOI":"10.1111\/j.1365-2990.1990.tb01156.x","article-title":"Glial and neuronal differentiation in the human fetal brain 9\u201323 weeks of gestation","volume":"16","author":"Wilkinson","year":"1990","journal-title":"Neuropathol. Appl. Neurobiol."},{"key":"10.1016\/j.jneumeth.2024.110085_bib73","doi-asserted-by":"crossref","first-page":"137","DOI":"10.1016\/j.jim.2007.01.020","article-title":"An evaluation of the immunohistochemistry benefits of boric acid antigen retrieval on rat decalcified joint tissues","volume":"322","author":"Wilson","year":"2007","journal-title":"J. Immunol. Methods"},{"key":"10.1016\/j.jneumeth.2024.110085_bib74","doi-asserted-by":"crossref","first-page":"841","DOI":"10.1097\/NEN.0b013e31822f471c","article-title":"Late Development of the GABAergic System in the Human Cerebral Cortex and White Matter","volume":"70","author":"Xu","year":"2011","journal-title":"J. Neuropathol. Exp. Neurol."},{"key":"10.1016\/j.jneumeth.2024.110085_bib75","doi-asserted-by":"crossref","first-page":"267","DOI":"10.1016\/0306-4522(95)00341-F","article-title":"Immunohistochemical studies of GABA and parvalbumin in the developing human cerebellum","volume":"70","author":"Yu","year":"1996","journal-title":"Neuroscience"},{"key":"10.1016\/j.jneumeth.2024.110085_bib76","doi-asserted-by":"crossref","first-page":"18","DOI":"10.1002\/dneu.20812","article-title":"Interneurons in the developing human neocortex","volume":"71","author":"Zecevic","year":"2011","journal-title":"Dev. Neurobiol."}],"container-title":["Journal of Neuroscience Methods"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S016502702400030X?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S016502702400030X?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,9,28]],"date-time":"2025-09-28T01:06:47Z","timestamp":1759021607000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S016502702400030X"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,5]]},"references-count":76,"alternative-id":["S016502702400030X"],"URL":"https:\/\/doi.org\/10.1016\/j.jneumeth.2024.110085","relation":{},"ISSN":["0165-0270"],"issn-type":[{"value":"0165-0270","type":"print"}],"subject":[],"published":{"date-parts":[[2024,5]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Systematic development of immunohistochemistry protocol for large cryosections-specific to non-perfused fetal brain","name":"articletitle","label":"Article Title"},{"value":"Journal of Neuroscience Methods","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.jneumeth.2024.110085","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2024 Elsevier B.V. All rights reserved.","name":"copyright","label":"Copyright"}],"article-number":"110085"}}