{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,17]],"date-time":"2026-05-17T09:09:07Z","timestamp":1779008947043,"version":"3.51.4"},"reference-count":24,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2026,6,1]],"date-time":"2026-06-01T00:00:00Z","timestamp":1780272000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2026,6,1]],"date-time":"2026-06-01T00:00:00Z","timestamp":1780272000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2026,6,1]],"date-time":"2026-06-01T00:00:00Z","timestamp":1780272000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-017"},{"start":{"date-parts":[[2026,6,1]],"date-time":"2026-06-01T00:00:00Z","timestamp":1780272000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2026,6,1]],"date-time":"2026-06-01T00:00:00Z","timestamp":1780272000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-012"},{"start":{"date-parts":[[2026,6,1]],"date-time":"2026-06-01T00:00:00Z","timestamp":1780272000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2026,6,1]],"date-time":"2026-06-01T00:00:00Z","timestamp":1780272000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-004"}],"funder":[{"DOI":"10.13039\/501100013105","name":"Shanghai Rising-Star Program","doi-asserted-by":"publisher","award":["23YF1418200"],"award-info":[{"award-number":["23YF1418200"]}],"id":[{"id":"10.13039\/501100013105","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100017950","name":"Shanghai Municipal Health Commission","doi-asserted-by":"publisher","award":["20234Y0294"],"award-info":[{"award-number":["20234Y0294"]}],"id":[{"id":"10.13039\/100017950","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100015378","name":"Shanghai University of Medicine and Health Sciences","doi-asserted-by":"publisher","award":["A1-0200-25-311007"],"award-info":[{"award-number":["A1-0200-25-311007"]}],"id":[{"id":"10.13039\/501100015378","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100015378","name":"Shanghai University of Medicine and Health Sciences","doi-asserted-by":"publisher","award":["XJJJ2501-3"],"award-info":[{"award-number":["XJJJ2501-3"]}],"id":[{"id":"10.13039\/501100015378","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Journal of Neuroscience Methods"],"published-print":{"date-parts":[[2026,6]]},"DOI":"10.1016\/j.jneumeth.2026.110718","type":"journal-article","created":{"date-parts":[[2026,2,24]],"date-time":"2026-02-24T07:42:25Z","timestamp":1771918945000},"page":"110718","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":0,"special_numbering":"C","title":["A single-stitch technique on the internal carotid artery for establishing the MCAO model"],"prefix":"10.1016","volume":"430","author":[{"given":"Xiaoming","family":"Xin","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chengji","family":"Wang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Song","family":"Tian","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Liang","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tongqing","family":"Duan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lingjuan","family":"Wu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Meichen","family":"Liu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jiaxing","family":"Dai","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tian","family":"Liu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mingyi","family":"Zheng","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lei","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ruling","family":"Shen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qiuqin","family":"Han","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1016\/j.jneumeth.2026.110718_bib1","doi-asserted-by":"crossref","unstructured":"Amado B., Melo L., Pinto R., Lobo A., Barros P., Gomes J.R. Ischemic Stroke, Lessons from the Past towards Effective Preclinical Models. 2022;10(10):2561.","DOI":"10.3390\/biomedicines10102561"},{"issue":"5","key":"10.1016\/j.jneumeth.2026.110718_bib2","doi-asserted-by":"crossref","first-page":"386","DOI":"10.1111\/j.1747-4949.2012.00802.x","article-title":"The influence of stroke risk factors and comorbidities on assessment of stroke therapies in humans and animals","volume":"7","author":"Ankolekar","year":"2012","journal-title":"Int. J. Stroke Off. J. Int. Stroke Soc."},{"issue":"9","key":"10.1016\/j.jneumeth.2026.110718_bib3","doi-asserted-by":"crossref","first-page":"1616","DOI":"10.1161\/01.STR.27.9.1616","article-title":"Middle cerebral artery occlusion in the rat by intraluminal suture. Neurological and pathological evaluation of an improved model","volume":"27","author":"Belayev","year":"1996","journal-title":"Stroke"},{"issue":"48","key":"10.1016\/j.jneumeth.2026.110718_bib4","article-title":"Mouse model of middle cerebral artery occlusion","author":"Chiang","year":"2011","journal-title":"J. Vis. Exp. JoVE"},{"issue":"5","key":"10.1016\/j.jneumeth.2026.110718_bib5","doi-asserted-by":"crossref","first-page":"260","DOI":"10.1016\/j.tem.2023.02.005","article-title":"Neuroendocrine regulation in stroke","volume":"34","author":"Datta","year":"2023","journal-title":"Trends Endocrinol. & Metab."},{"issue":"2","key":"10.1016\/j.jneumeth.2026.110718_bib6","doi-asserted-by":"crossref","first-page":"372","DOI":"10.1016\/j.expneurol.2005.05.013","article-title":"The role of ECA transection in the development of masticatory lesions in the MCAO filament model","volume":"195","author":"Dittmar","year":"2005","journal-title":"Exp. Neurol."},{"key":"10.1016\/j.jneumeth.2026.110718_bib7","first-page":"3445","article-title":"Animal models of ischemic stroke and their application in clinical research","volume":"9","author":"Fluri","year":"2015","journal-title":"Drug Des. Dev. Ther."},{"issue":"4","key":"10.1016\/j.jneumeth.2026.110718_bib8","doi-asserted-by":"crossref","first-page":"413","DOI":"10.1136\/neurintsurg-2021-017370","article-title":"Endovascular middle cerebral artery embolic stroke model: a novel approach","volume":"14","author":"Gonzalez","year":"2022","journal-title":"J. neurointerventional Surg."},{"issue":"8","key":"10.1016\/j.jneumeth.2026.110718_bib9","doi-asserted-by":"crossref","first-page":"2405","DOI":"10.1007\/s11011-021-00818-0","article-title":"Gene expression profiles in the brain of phenylketonuria mouse model reversed by the low phenylalanine diet therapy","volume":"36","author":"Hong","year":"2021","journal-title":"Metab. brain Dis."},{"key":"10.1016\/j.jneumeth.2026.110718_bib10","doi-asserted-by":"crossref","DOI":"10.1016\/j.biopha.2024.117564","article-title":"Advances in nanoparticle-based therapeutics for ischemic stroke: Enhancing drug delivery and efficacy","volume":"180","author":"Ji","year":"2024","journal-title":"Biomed. & Pharmacother."},{"key":"10.1016\/j.jneumeth.2026.110718_bib11","doi-asserted-by":"crossref","first-page":"354","DOI":"10.1016\/j.nbd.2013.10.011","article-title":"Treatment with afobazole at delayed time points following ischemic stroke improves long-term functional and histological outcomes","volume":"62","author":"Katnik","year":"2014","journal-title":"Neurobiol. Dis."},{"key":"10.1016\/j.jneumeth.2026.110718_bib12","doi-asserted-by":"crossref","first-page":"35","DOI":"10.1016\/j.brainresbull.2016.02.016","article-title":"A review on animal models of stroke: An update","volume":"122","author":"Kumar","year":"2016","journal-title":"Brain Res. Bull."},{"key":"10.1016\/j.jneumeth.2026.110718_bib13","article-title":"Middle cerebral artery occlusion model in rodents: methods and potential pitfalls","volume":"2011","author":"Liu","year":"2011","journal-title":"J. Biomed. & Biotechnol."},{"issue":"1","key":"10.1016\/j.jneumeth.2026.110718_bib14","doi-asserted-by":"crossref","first-page":"84","DOI":"10.1161\/01.STR.20.1.84","article-title":"Reversible middle cerebral artery occlusion without craniectomy in rats","volume":"20","author":"Longa","year":"1989","journal-title":"Stroke"},{"issue":"1","key":"10.1016\/j.jneumeth.2026.110718_bib15","doi-asserted-by":"crossref","first-page":"78","DOI":"10.1007\/s12031-014-0253-3","article-title":"Changes in thyroid hormone receptors after permanent cerebral ischemia in male rats","volume":"54","author":"Lourbopoulos","year":"2014","journal-title":"Journal molecular neuroscience MN"},{"key":"10.1016\/j.jneumeth.2026.110718_bib16","doi-asserted-by":"crossref","DOI":"10.1016\/j.expneurol.2024.114942","article-title":"In vitro modelling of the neurovascular unit for ischemic stroke research: Emphasis on human cell applications and 3D model design","volume":"381","author":"Pang","year":"2024","journal-title":"Exp. Neurol."},{"key":"10.1016\/j.jneumeth.2026.110718_bib17","first-page":"145","article-title":"Nanotechnology approaches to drug delivery for the treatment of ischemic stroke","volume":"43","author":"Peng","year":"2025","journal-title":"Bioact. Mater."},{"key":"10.1016\/j.jneumeth.2026.110718_bib18","doi-asserted-by":"crossref","unstructured":"Philouze P., Malard O., Albert S., et al. A New Animal Model of Laryngeal Transplantation. 2022;11(21):6427.","DOI":"10.3390\/jcm11216427"},{"key":"10.1016\/j.jneumeth.2026.110718_bib19","doi-asserted-by":"crossref","DOI":"10.3389\/fneur.2022.846735","article-title":"Bridging the Transient Intraluminal Stroke Preclinical Model to Clinical Practice: From Improved Surgical Procedures to a Workflow of Functional Tests","volume":"13","author":"Pinto","year":"2022","journal-title":"Front. Neurol."},{"key":"10.1016\/j.jneumeth.2026.110718_bib20","doi-asserted-by":"crossref","DOI":"10.1016\/j.brainresbull.2024.110983","article-title":"Thyroid hormones and stroke, the gap between clinical and experimental studies","volume":"213","author":"Shafia","year":"2024","journal-title":"Brain Res. Bull."},{"key":"10.1016\/j.jneumeth.2026.110718_bib21","article-title":"Preclinical models of middle cerebral artery occlusion: new imaging approaches to a classic technique","volume":"14","author":"Sokolowski","year":"2023","journal-title":"Rev. Front. Neurol."},{"issue":"4","key":"10.1016\/j.jneumeth.2026.110718_bib22","doi-asserted-by":"crossref","first-page":"651","DOI":"10.1007\/s12975-011-0102-4","article-title":"A Critical Re-Examination of the Intraluminal Filament MCAO Model: Impact of External Carotid Artery Transection","volume":"2","author":"Trueman","year":"2011","journal-title":"Transl. Stroke Res."},{"key":"10.1016\/j.jneumeth.2026.110718_bib23","article-title":"An Optimal Animal Model of Ischemic Stroke Established by Digital Subtraction Angiography-Guided Autologous Thrombi in Cynomolgus Monkeys","volume":"13","author":"Ye","year":"2022","journal-title":"Orig. Res."},{"key":"10.1016\/j.jneumeth.2026.110718_bib24","doi-asserted-by":"crossref","unstructured":"Zeng L., Hu S., Zeng J.Y., Hongkuan %J brain sciences. Animal Models of Ischemic Stroke with Different Forms of Middle Cerebral Artery Occlusion. 2023;13(7).","DOI":"10.3390\/brainsci13071007"}],"container-title":["Journal of Neuroscience Methods"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0165027026000488?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0165027026000488?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,5,17]],"date-time":"2026-05-17T08:29:16Z","timestamp":1779006556000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0165027026000488"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,6]]},"references-count":24,"alternative-id":["S0165027026000488"],"URL":"https:\/\/doi.org\/10.1016\/j.jneumeth.2026.110718","relation":{},"ISSN":["0165-0270"],"issn-type":[{"value":"0165-0270","type":"print"}],"subject":[],"published":{"date-parts":[[2026,6]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"A single-stitch technique on the internal carotid artery for establishing the MCAO model","name":"articletitle","label":"Article Title"},{"value":"Journal of Neuroscience Methods","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.jneumeth.2026.110718","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2026 Elsevier B.V. All rights are reserved, including those for text and data mining, AI training, and similar technologies.","name":"copyright","label":"Copyright"}],"article-number":"110718"}}