{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,9]],"date-time":"2026-09-09T11:14:36Z","timestamp":1788952476096,"version":"build-2803163510"},"reference-count":30,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2026,12,1]],"date-time":"2026-12-01T00:00:00Z","timestamp":1796083200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2026,12,1]],"date-time":"2026-12-01T00:00:00Z","timestamp":1796083200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2026,8,24]],"date-time":"2026-08-24T00:00:00Z","timestamp":1787529600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001834","name":"University of Twente","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001834","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Journal of Neuroscience Methods"],"published-print":{"date-parts":[[2026,12]]},"DOI":"10.1016\/j.jneumeth.2026.110882","type":"journal-article","created":{"date-parts":[[2026,8,22]],"date-time":"2026-08-22T14:42:57Z","timestamp":1787409777000},"page":"110882","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":0,"special_numbering":"C","title":["Cryopreservation of rat cortical cells for brain-on-chip research"],"prefix":"10.1016","volume":"436","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7196-200X","authenticated-orcid":false,"given":"Gerco C.","family":"Hassink","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marloes R.","family":"Levers","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8319-3626","authenticated-orcid":false,"given":"Michel J.A.M.","family":"van Putten","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.jneumeth.2026.110882_b1","doi-asserted-by":"crossref","DOI":"10.1016\/j.jneumeth.2023.110034","article-title":"Method for cryopreservation of trigeminal ganglion for establishing primary cultures of neurons and glia","volume":"402","author":"Antonopoulos","year":"2024","journal-title":"J. Neurosci. Methods"},{"key":"10.1016\/j.jneumeth.2026.110882_b2","series-title":"A primary rat neuron-astrocyte-microglia tri-culture model for studying mechanisms of neurotoxicity","author":"Badley","year":"2025"},{"key":"10.1016\/j.jneumeth.2026.110882_b3","doi-asserted-by":"crossref","DOI":"10.1038\/s42003-025-08209-2","article-title":"Automated inference of disease mechanisms in patient-hiPSC-derived neuronal networks Check for updates","volume":"8","author":"Doorn","year":"2025","journal-title":"Commun. Biology"},{"issue":"11","key":"10.1016\/j.jneumeth.2026.110882_b4","doi-asserted-by":"crossref","first-page":"1583","DOI":"10.1016\/j.stemcr.2024.09.001","article-title":"Breaking the burst: Unveiling mechanisms behind fragmented network bursts in patient-derived neurons","volume":"19","author":"Doorn","year":"2024","journal-title":"Stem Cell Rep."},{"issue":"119","key":"10.1016\/j.jneumeth.2026.110882_b5","first-page":"1","article-title":"Rapid neuronal differentiation of induced pluripotent stem cells for measuring network activity on micro-electrode arrays","author":"Frega","year":"2017","journal-title":"J. Vis. Exp."},{"issue":"JUN","key":"10.1016\/j.jneumeth.2026.110882_b6","article-title":"Enhancement of cortical network activity in vitro and promotion of GABAergic neurogenesis by stimulation with an electromagnetic field with a 150 MHz carrier wave pulsed with an alternating 10 and 16 Hz modulation","volume":"6","author":"Gramowski-Vo\u00df","year":"2015","journal-title":"Front. Neurol."},{"issue":"1","key":"10.1016\/j.jneumeth.2026.110882_b7","doi-asserted-by":"crossref","first-page":"10","DOI":"10.1186\/s13041-015-0099-9","article-title":"Ontogeny of biochemical, morphological and functional parameters of synaptogenesis in primary cultures of rat hippocampal and cortical neurons","volume":"8","author":"Harrill","year":"2015","journal-title":"Mol. Brain"},{"issue":"4","key":"10.1016\/j.jneumeth.2026.110882_b8","doi-asserted-by":"crossref","first-page":"387","DOI":"10.1254\/jphs.SC0070162","article-title":"Cryopreservation of granule cells from the postnatal rat hippocampus","volume":"104","author":"Ichikawa","year":"2007","journal-title":"J. Pharmacol. Sci."},{"issue":"September 2019","key":"10.1016\/j.jneumeth.2026.110882_b9","article-title":"A simple DMSO-based method for cryopreservation of primary hippocampal and cortical neurons","volume":"333","author":"Ishizuka","year":"2020","journal-title":"J. Neurosci. Methods"},{"key":"10.1016\/j.jneumeth.2026.110882_b10","series-title":"Bridging the gap in Parkinson\u2019s disease research: from general pathophysiology to advanced 3d cell culture models","author":"Keles","year":"2025"},{"key":"10.1016\/j.jneumeth.2026.110882_b11","series-title":"Prediction in cultured cortical neural networks","author":"Lamberti","year":"2023"},{"key":"10.1016\/j.jneumeth.2026.110882_b12","series-title":"Advances in Neurobiology","doi-asserted-by":"crossref","DOI":"10.1007\/978-3-030-11135-9_2","article-title":"In vitro models of brain disorders","volume":"Vol. 22","author":"le Feber","year":"2019"},{"issue":"8","key":"10.1016\/j.jneumeth.2026.110882_b13","doi-asserted-by":"crossref","first-page":"3443","DOI":"10.1016\/j.bpj.2009.01.013","article-title":"Latency-related development of functional connections in cultured cortical networks","volume":"96","author":"Le Feber","year":"2009","journal-title":"Biophys. J."},{"key":"10.1016\/j.jneumeth.2026.110882_b14","doi-asserted-by":"crossref","DOI":"10.1007\/s13365-025-01283-6","article-title":"SARS-CoV-2-induced damage to rat cortical neuronal networks ex vivo is mediated by the pro-inflammatory activation of the cGAS-STING pathway","author":"Martinez-Orellana","year":"2025","journal-title":"J. NeuroVirology"},{"key":"10.1016\/j.jneumeth.2026.110882_b15","doi-asserted-by":"crossref","unstructured":"Mat, M.P., Son, Kater, S.B., 1988. Isolated Hippocampal Neurons in Cryopreserved Long-Term Cultures: Development of Neuroarchitecture and Sensitivity to NMDA. Tech. rep., Vol. 5, pp. 439\u2013452.","DOI":"10.1016\/0736-5748(88)90050-0"},{"issue":"14","key":"10.1016\/j.jneumeth.2026.110882_b16","doi-asserted-by":"crossref","DOI":"10.3390\/cells12141914","article-title":"Application-oriented bulk cryopreservation of human iPSCs in Cryo bags followed by direct inoculation in scalable suspension bioreactors for expansion and neural differentiation","volume":"12","author":"Meiser","year":"2023","journal-title":"Cells"},{"issue":"2","key":"10.1016\/j.jneumeth.2026.110882_b17","doi-asserted-by":"crossref","first-page":"284","DOI":"10.1016\/j.jneumeth.2009.02.009","article-title":"A device to facilitate preparation of high-density neural cell cultures in MEAs","volume":"179","author":"Mok","year":"2009","journal-title":"J. Neurosci. Methods"},{"issue":"1\u20132","key":"10.1016\/j.jneumeth.2026.110882_b18","doi-asserted-by":"crossref","first-page":"133","DOI":"10.1016\/j.jneumeth.2003.08.006","article-title":"Primary culture of cortical neurons, type-1 astrocytes, and microglial cells from cynomolgus monkey (Macaca fascicularis) fetuses","volume":"131","author":"Negishi","year":"2003","journal-title":"J. Neurosci. Methods"},{"key":"10.1016\/j.jneumeth.2026.110882_b19","series-title":"Cell culture models for epilepsy research and treatment","author":"Oblasov","year":"2024"},{"key":"10.1016\/j.jneumeth.2026.110882_b20","doi-asserted-by":"crossref","DOI":"10.7554\/eLife.47996","article-title":"Self-organization of modular network architecture by activity-dependent neuronal migration and outgrowth","volume":"8","author":"Okujeni","year":"2019","journal-title":"ELife"},{"issue":"5","key":"10.1016\/j.jneumeth.2026.110882_b21","doi-asserted-by":"crossref","DOI":"10.1523\/ENEURO.0135-18.2018","article-title":"High fidelity cryopreservation and recovery of primary rodent cortical neurons","volume":"5","author":"Parker","year":"2018","journal-title":"ENeuro"},{"key":"10.1016\/j.jneumeth.2026.110882_b22","doi-asserted-by":"crossref","DOI":"10.3389\/fncel.2018.00081","article-title":"Cryopreservation of primary mouse neurons: The benefit of neurostore cryoprotective medium","volume":"12","author":"Pischedda","year":"2018","journal-title":"Front. Cell. Neurosci."},{"issue":"3","key":"10.1016\/j.jneumeth.2026.110882_b23","doi-asserted-by":"crossref","first-page":"419","DOI":"10.1007\/s10616-014-9700-9","article-title":"Freshly frozen E18 rat cortical cells can generate functional neural networks after standard cryopreservation and thawing procedures","volume":"67","author":"Quasthoff","year":"2015","journal-title":"Cytotechnology"},{"issue":"3","key":"10.1016\/j.jneumeth.2026.110882_b24","doi-asserted-by":"crossref","first-page":"191","DOI":"10.1016\/j.cryobiol.2016.05.003","article-title":"Cryopreservation by slow cooling of rat neuronal cells","volume":"72","author":"Robert","year":"2016","journal-title":"Cryobiology"},{"key":"10.1016\/j.jneumeth.2026.110882_b25","series-title":"Fiji: An open-source platform for biological-image analysis","author":"Schindelin","year":"2012"},{"issue":"2","key":"10.1016\/j.jneumeth.2026.110882_b26","doi-asserted-by":"crossref","first-page":"324","DOI":"10.1016\/j.jneumeth.2012.01.016","article-title":"Development of dissociated cryopreserved rat cortical neurons in vitro","volume":"205","author":"Schock","year":"2012","journal-title":"J. Neurosci. Methods"},{"issue":"4","key":"10.1016\/j.jneumeth.2026.110882_b27","doi-asserted-by":"crossref","first-page":"1021","DOI":"10.1007\/s10439-023-03438-0","article-title":"Stretch-induced injury affects cortical neuronal networks in a time- and severity-dependent manner","volume":"52","author":"Sullivan","year":"2024","journal-title":"Ann. Biomed. Eng."},{"key":"10.1016\/j.jneumeth.2026.110882_b28","doi-asserted-by":"crossref","first-page":"3137","DOI":"10.1007\/s10571-023-01368-y","article-title":"Neuronal responses to ischemia: Scoping review of insights from human-derived in vitro models","volume":"43","author":"Voogd","year":"2023","journal-title":"Mol. Neurobiol."},{"key":"10.1016\/j.jneumeth.2026.110882_b29","doi-asserted-by":"crossref","DOI":"10.3389\/fcell.2022.937761","article-title":"Spontaneous activity predicts survival of developing cortical neurons","volume":"10","author":"Warm","year":"2022","journal-title":"Front. Cell Dev. Biology"},{"issue":"6","key":"10.1016\/j.jneumeth.2026.110882_b30","doi-asserted-by":"crossref","DOI":"10.3892\/etm.2020.9285","article-title":"A modified protocol for the isolation, culture, and cryopreservation of rat embryonic neural stem cells","volume":"20","author":"Zhou","year":"2020","journal-title":"Exp. Ther. Med."}],"container-title":["Journal of Neuroscience Methods"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0165027026002128?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0165027026002128?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,9,9]],"date-time":"2026-09-09T10:36:56Z","timestamp":1788950216000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0165027026002128"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,12]]},"references-count":30,"alternative-id":["S0165027026002128"],"URL":"https:\/\/doi.org\/10.1016\/j.jneumeth.2026.110882","relation":{},"ISSN":["0165-0270"],"issn-type":[{"value":"0165-0270","type":"print"}],"subject":[],"published":{"date-parts":[[2026,12]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Cryopreservation of rat cortical cells for brain-on-chip research","name":"articletitle","label":"Article Title"},{"value":"Journal of Neuroscience Methods","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.jneumeth.2026.110882","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2026 The Authors. Published by Elsevier B.V.","name":"copyright","label":"Copyright"}],"article-number":"110882"}}