{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,7]],"date-time":"2026-02-07T17:02:53Z","timestamp":1770483773317,"version":"3.49.0"},"reference-count":50,"publisher":"Elsevier BV","issue":"4","license":[{"start":{"date-parts":[[2025,12,1]],"date-time":"2025-12-01T00:00:00Z","timestamp":1764547200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2025,12,1]],"date-time":"2025-12-01T00:00:00Z","timestamp":1764547200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2025,10,28]],"date-time":"2025-10-28T00:00:00Z","timestamp":1761609600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["24K12953"],"award-info":[{"award-number":["24K12953"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["22K09972"],"award-info":[{"award-number":["22K09972"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["25K12991"],"award-info":[{"award-number":["25K12991"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["22K17025"],"award-info":[{"award-number":["22K17025"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["25K13024"],"award-info":[{"award-number":["25K13024"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Journal of Oral Biosciences"],"published-print":{"date-parts":[[2025,12]]},"DOI":"10.1016\/j.job.2025.100705","type":"journal-article","created":{"date-parts":[[2025,11,1]],"date-time":"2025-11-01T03:38:16Z","timestamp":1761968296000},"page":"100705","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":1,"title":["Piezo1 negatively regulates proliferation, but enhances mineralization in human cementoblasts"],"prefix":"10.1016","volume":"67","author":[{"ORCID":"https:\/\/orcid.org\/0009-0000-0104-9445","authenticated-orcid":false,"given":"Toshihiro","family":"Hasegawa","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4258-2152","authenticated-orcid":false,"given":"Takehito","family":"Ouchi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ryuya","family":"Kurashima","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maki","family":"Kimura","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Seiji","family":"Asoda","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Taneaki","family":"Nakagawa","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yoshiyuki","family":"Shibukawa","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1016\/j.job.2025.100705_bib1","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1111\/j.1600-0757.1997.tb00093.x","article-title":"The development of the periodontium--a largely ectomesenchymally derived unit","volume":"13","author":"Ten Cate","year":"2000","journal-title":"Periodontol"},{"key":"10.1016\/j.job.2025.100705_bib2","doi-asserted-by":"crossref","first-page":"2024","DOI":"10.3390\/ijms17122024","article-title":"Screening the expression changes in MicroRNAs and their target genes in mature cementoblasts stimulated with cyclic tensile stress","volume":"17","author":"Wang","year":"2016","journal-title":"Int J Mol Sci"},{"key":"10.1016\/j.job.2025.100705_bib3","doi-asserted-by":"crossref","first-page":"1238","DOI":"10.1016\/j.archoralbio.2011.05.007","article-title":"Effect of PGE2 induced by compressive and tensile stresses on cementoblast differentiation in vitro","volume":"56","author":"Rego","year":"2011","journal-title":"Arch Oral Biol"},{"key":"10.1016\/j.job.2025.100705_bib4","doi-asserted-by":"crossref","first-page":"211","DOI":"10.1111\/prd.12062","article-title":"Cementum proteins: role in cementogenesis, biomineralization, periodontium formation and regeneration","volume":"67","author":"Arzate","year":"2000","journal-title":"Periodontol"},{"key":"10.1016\/j.job.2025.100705_bib5","doi-asserted-by":"crossref","first-page":"144","DOI":"10.3390\/dj10080144","article-title":"Characterization of SIBLING proteins in the mineralized tissues","volume":"10","author":"Dab","year":"2022","journal-title":"Dent J (Basel)"},{"key":"10.1016\/j.job.2025.100705_bib6","doi-asserted-by":"crossref","first-page":"649","DOI":"10.1002\/jcp.22770","article-title":"Cementum protein 1 (CEMP1) induces a cementoblastic phenotype and reduces osteoblastic differentiation in periodontal ligament cells","volume":"227","author":"Komaki","year":"2012","journal-title":"J Cell Physiol"},{"key":"10.1016\/j.job.2025.100705_bib7","doi-asserted-by":"crossref","first-page":"639","DOI":"10.1016\/j.devcel.2020.06.006","article-title":"Gli1+ periodontium stem cells are regulated by osteocytes and occlusal force","volume":"54","author":"Men","year":"2020","journal-title":"Dev Cell"},{"key":"10.1016\/j.job.2025.100705_bib8","doi-asserted-by":"crossref","first-page":"1289","DOI":"10.1177\/00220345211007429","article-title":"A biphasic feature of Gli1+-Mesenchymal progenitors during cementogenesis that is positively controlled by Wnt\/\u03b2-Catenin signaling","volume":"100","author":"Xie","year":"2021","journal-title":"J Dent Res"},{"key":"10.1016\/j.job.2025.100705_bib9","doi-asserted-by":"crossref","first-page":"1809","DOI":"10.1093\/jbmr\/zjae152","article-title":"Deciphering the spatial distribution of Gli1-lineage cells in dental, oral, and craniofacial regions","volume":"39","author":"Li","year":"2024","journal-title":"J Bone Miner Res"},{"key":"10.1016\/j.job.2025.100705_bib10","doi-asserted-by":"crossref","first-page":"6061","DOI":"10.1038\/s41467-025-61050-3","article-title":"A Hedgehog-Foxf axis coordinates dental follicle-derived alveolar bone formation","volume":"16","author":"Nagata","year":"2025","journal-title":"Nat Commun"},{"key":"10.1016\/j.job.2025.100705_bib11","doi-asserted-by":"crossref","first-page":"92","DOI":"10.1002\/stem.3288","article-title":"Stem cell contributions to cementoblast differentiation in healthy periodontal ligament and periodontitis","volume":"39","author":"Zhao","year":"2021","journal-title":"Stem Cell"},{"key":"10.1016\/j.job.2025.100705_bib12","doi-asserted-by":"crossref","DOI":"10.1038\/ncomms11277","article-title":"Parathyroid hormone receptor signalling in osterix-expressing mesenchymal progenitors is essential for tooth root formation","volume":"7","author":"Ono","year":"2016","journal-title":"Nat Commun"},{"key":"10.1016\/j.job.2025.100705_bib13","doi-asserted-by":"crossref","first-page":"5510","DOI":"10.1038\/s41467-025-61048-x","article-title":"Wnt-directed CXCL12-expressing apical papilla progenitor cells drive tooth root formation","volume":"16","author":"Nagata","year":"2025","journal-title":"Nat Commun"},{"key":"10.1016\/j.job.2025.100705_bib14","doi-asserted-by":"crossref","first-page":"41","DOI":"10.1111\/j.1600-0757.1997.tb00095.x","article-title":"Dental cementum: the dynamic tissue covering of the root","volume":"13","author":"Bosshardt","year":"2000","journal-title":"Periodontol"},{"key":"10.1016\/j.job.2025.100705_bib15","doi-asserted-by":"crossref","first-page":"1297","DOI":"10.1002\/JPER.18-0604","article-title":"Severe periodontitis may influence cementum and dental pulp through inflammation, oxidative stress, and apoptosis","volume":"90","author":"Li","year":"2019","journal-title":"J Periodontol"},{"key":"10.1016\/j.job.2025.100705_bib16","doi-asserted-by":"crossref","first-page":"1064","DOI":"10.1902\/jop.2010.090678","article-title":"17beta-estradiol promotes cementoblast proliferation and cementum formation in experimental periodontitis","volume":"81","author":"Nu\u00f1ez","year":"2010","journal-title":"J Periodontol"},{"key":"10.1016\/j.job.2025.100705_bib17","doi-asserted-by":"crossref","first-page":"189","DOI":"10.1902\/jop.1978.49.4.189","article-title":"A histologic study of age changes in the adult human periodontal joint (ligament)","volume":"49","author":"Severson","year":"1978","journal-title":"J Periodontol"},{"key":"10.1016\/j.job.2025.100705_bib18","doi-asserted-by":"crossref","first-page":"748","DOI":"10.1902\/jop.1985.56.12.748","article-title":"A new look at the etiology and pathogenesis of early-onset periodontitis. Cementopathia revisited","volume":"56","author":"Page","year":"1985","journal-title":"J Periodontol"},{"key":"10.1016\/j.job.2025.100705_bib19","doi-asserted-by":"crossref","first-page":"358","DOI":"10.4103\/0973-029X.86725","article-title":"Benign cementoblastoma","volume":"15","author":"Sankari","year":"2011","journal-title":"J Oral Maxillofac Pathol"},{"key":"10.1016\/j.job.2025.100705_bib20","doi-asserted-by":"crossref","first-page":"240","DOI":"10.1007\/s12663-019-01304-y","article-title":"Cemento-ossifying fibroma in maxillofacial region: a series of 16 cases","volume":"20","author":"Kaur","year":"2021","journal-title":"J Maxillofac Oral Surg"},{"key":"10.1016\/j.job.2025.100705_bib21","doi-asserted-by":"crossref","first-page":"138","DOI":"10.3390\/dj11050138","article-title":"Cemento-Osseous dysplasia of the jaw: Demographic and clinical analysis of 191 new cases","volume":"11","author":"Decolibus","year":"2023","journal-title":"Dent J (Basel)"},{"key":"10.1016\/j.job.2025.100705_bib22","doi-asserted-by":"crossref","DOI":"10.1002\/mgg3.2277","article-title":"Familial gigantiform cementoma with recurrent ANO5 p.Cys356Tyr mutations: clinicopathological and genetic study with literature review","volume":"12","author":"Zhou","year":"2024","journal-title":"Mol Genet Genomic Med"},{"key":"10.1016\/j.job.2025.100705_bib23","doi-asserted-by":"crossref","first-page":"e591","DOI":"10.4317\/medoral.26501","article-title":"Synchronous cemento-ossifying fibromas: a systematic review","volume":"29","author":"Neumann","year":"2024","journal-title":"Med Oral Patol Oral Cir Bucal"},{"key":"10.1016\/j.job.2025.100705_bib24","doi-asserted-by":"crossref","first-page":"1759","DOI":"10.1016\/j.jcms.2017.08.002","article-title":"Cementoblastoma: an updated analysis of 258 cases reported in the literature","volume":"45","author":"Chrcanovic","year":"2017","journal-title":"J Craniomaxillofac Surg"},{"key":"10.1016\/j.job.2025.100705_bib25","doi-asserted-by":"crossref","first-page":"133","DOI":"10.1007\/s12105-008-0099-5","article-title":"Cementoblastoma","volume":"3","author":"Huber","year":"2009","journal-title":"Head Neck Pathol"},{"key":"10.1016\/j.job.2025.100705_bib26","doi-asserted-by":"crossref","first-page":"282","DOI":"10.1038\/s41467-019-14146-6","article-title":"Mechanical sensing protein PIEZO1 regulates bone homeostasis via osteoblast-osteoclast crosstalk","volume":"11","author":"Wang","year":"2020","journal-title":"Nat Commun"},{"key":"10.1016\/j.job.2025.100705_bib27","doi-asserted-by":"crossref","first-page":"1","DOI":"10.2209\/tdcpublication.2022-0015","article-title":"Functional expression of mechanosensitive Piezo1\/TRPV4 channels in mouse osteoblasts","volume":"64","author":"Nagai","year":"2023","journal-title":"Bull Tokyo Dent Coll"},{"key":"10.1016\/j.job.2025.100705_bib28","doi-asserted-by":"crossref","first-page":"55","DOI":"10.1126\/science.1193270","article-title":"Piezo1 and Piezo2 are essential components of distinct mechanically activated cation channels","volume":"330","author":"Coste","year":"2010","journal-title":"Science"},{"key":"10.1016\/j.job.2025.100705_bib29","doi-asserted-by":"crossref","first-page":"617","DOI":"10.1038\/nature13250","article-title":"Epidermal Merkel cells are mechanosensory cells that tune mammalian touch receptors","volume":"509","author":"Maksimovic","year":"2014","journal-title":"Nature"},{"key":"10.1016\/j.job.2025.100705_bib30","doi-asserted-by":"crossref","first-page":"984","DOI":"10.1016\/j.joen.2018.02.020","article-title":"Activation of mechanosensitive transient receptor potential\/piezo channels in odontoblasts generates action potentials in cocultured isolectin B4-negative medium-sized trigeminal ganglion neurons","volume":"44","author":"Sato","year":"2018","journal-title":"J Endod"},{"key":"10.1016\/j.job.2025.100705_bib31","doi-asserted-by":"crossref","DOI":"10.3389\/fphys.2021.704518","article-title":"Mechanical stimulation-induced calcium signaling by Piezo1 channel activation in human odontoblast reduces dentin mineralization","volume":"12","author":"Matsunaga","year":"2021","journal-title":"Front Physiol"},{"key":"10.1016\/j.job.2025.100705_bib32","doi-asserted-by":"crossref","DOI":"10.3389\/fphys.2022.891759","article-title":"Piezo1-pannexin-1-P2X3 axis in odontoblasts and neurons mediates sensory transduction in dentinal sensitivity","volume":"13","author":"Ohyama","year":"2022","journal-title":"Front Physiol"},{"key":"10.1016\/j.job.2025.100705_bib33","doi-asserted-by":"crossref","first-page":"656","DOI":"10.1177\/00220345241307944","article-title":"Pericytes are odontoblast progenitor cells depending on ER stress","volume":"104","author":"Ouchi","year":"2025","journal-title":"J Dent Res"},{"key":"10.1016\/j.job.2025.100705_bib34","doi-asserted-by":"crossref","first-page":"1035","DOI":"10.1016\/j.bone.2006.05.022","article-title":"Characterization of established cementoblast-like cell lines from human cementum-lining cells in vitro and in vivo","volume":"39","author":"Kitagawa","year":"2006","journal-title":"Bone"},{"key":"10.1016\/j.job.2025.100705_bib35","first-page":"1","article-title":"Increased Piezo1 channel activity in interstitial Cajal-like cells induces bladder hyperactivity by functionally interacting with NCX1 in rats with cyclophosphamide-induced cystitis","volume":"50","author":"Liu","year":"2018","journal-title":"Exp Mol Med"},{"key":"10.1016\/j.job.2025.100705_bib36","doi-asserted-by":"crossref","first-page":"3834","DOI":"10.3390\/ijms21113834","article-title":"Functional expression of Piezo1 in Dorsal Root Ganglion (DRG) neurons","volume":"21","author":"Roh","year":"2020","journal-title":"Int J Mol Sci"},{"key":"10.1016\/j.job.2025.100705_bib37","doi-asserted-by":"crossref","DOI":"10.7554\/eLife.07369","article-title":"Chemical activation of the mechanotransduction channel Piezo1","volume":"4","author":"Syeda","year":"2015","journal-title":"eLife"},{"key":"10.1016\/j.job.2025.100705_bib38","doi-asserted-by":"crossref","first-page":"1744","DOI":"10.1111\/bph.14188","article-title":"Yoda1 analogue (Dooku1) which antagonizes Yoda1-evoked activation of Piezo1 and aortic relaxation","volume":"175","author":"Evans","year":"2018","journal-title":"Br J Pharmacol"},{"key":"10.1016\/j.job.2025.100705_bib39","doi-asserted-by":"crossref","first-page":"326","DOI":"10.3389\/fphys.2015.00326","article-title":"Intercellular odontoblast communication via ATP mediated by Pannexin-1 channel and phospholipase C-coupled receptor activation","volume":"6","author":"Sato","year":"2015","journal-title":"Front Physiol"},{"key":"10.1016\/j.job.2025.100705_bib40","doi-asserted-by":"crossref","first-page":"843","DOI":"10.1007\/s00424-014-1551-x","article-title":"Odontoblasts as sensory receptors: transient receptor potential channels, pannexin-1, and ionotropic ATP receptors mediate intercellular odontoblast-neuron signal transduction","volume":"467","author":"Shibukawa","year":"2015","journal-title":"Pfl\u00fcgers Archiv"},{"key":"10.1016\/j.job.2025.100705_bib41","doi-asserted-by":"crossref","first-page":"341","DOI":"10.1016\/j.ceca.2016.07.003","article-title":"Intercellular signal communication among odontoblasts and trigeminal ganglion neurons via glutamate","volume":"60","author":"Nishiyama","year":"2016","journal-title":"Cell Calcium"},{"key":"10.1016\/j.job.2025.100705_bib42","doi-asserted-by":"crossref","first-page":"1057","DOI":"10.1177\/0022034516644702","article-title":"High pH-Sensitive TRPA1 activation in odontoblasts regulates mineralization","volume":"95","author":"Kimura","year":"2016","journal-title":"J Dent Res"},{"key":"10.1016\/j.job.2025.100705_bib43","doi-asserted-by":"crossref","first-page":"2483","DOI":"10.1084\/jem.20091046","article-title":"Prospective identification, isolation, and systemic transplantation of multipotent mesenchymal stem cells in murine bone marrow","volume":"206","author":"Morikawa","year":"2009","journal-title":"J Exp Med"},{"key":"10.1016\/j.job.2025.100705_bib44","doi-asserted-by":"crossref","DOI":"10.7554\/eLife.03696","article-title":"The neural crest is a source of mesenchymal stem cells with specialized hematopoietic stem cell niche function","volume":"3","author":"Isern","year":"2014","journal-title":"eLife"},{"key":"10.1016\/j.job.2025.100705_bib45","doi-asserted-by":"crossref","first-page":"618","DOI":"10.2319\/110616-799.1","article-title":"Cementogenesis is inhibited under a mechanical static compressive force via Piezo1","volume":"87","author":"Zhang","year":"2017","journal-title":"Angle Orthod"},{"key":"10.1016\/j.job.2025.100705_bib46","doi-asserted-by":"crossref","DOI":"10.1016\/j.job.2025.100651","article-title":"Piezo1 promotes double-directional differentiation from human periodontal ligament progenitor cells","volume":"67","author":"Kono","year":"2025","journal-title":"J Oral Biosci"},{"key":"10.1016\/j.job.2025.100705_bib47","doi-asserted-by":"crossref","first-page":"72","DOI":"10.1016\/j.reth.2019.12.011","article-title":"Mesenchymal stem cell-based tissue regeneration therapies for periodontitis","volume":"14","author":"Ouchi","year":"2020","journal-title":"Regen Ther"},{"key":"10.1016\/j.job.2025.100705_bib48","doi-asserted-by":"crossref","first-page":"102","DOI":"10.1111\/prd.12029","article-title":"Extracellular matrix mineralization in periodontal tissues: noncollagenous matrix proteins, enzymes, and relationship to hypophosphatasia and X-linked hypophosphatemia","volume":"63","author":"McKee","year":"2000","journal-title":"Periodontol"},{"key":"10.1016\/j.job.2025.100705_bib49","doi-asserted-by":"crossref","DOI":"10.1016\/j.acthis.2022.151868","article-title":"Bmal1 promotes cementoblast differentiation and cementum mineralization via Wnt\/\u03b2-catenin signaling","volume":"124","author":"Liu","year":"2022","journal-title":"Acta Histochem"},{"key":"10.1016\/j.job.2025.100705_bib50","article-title":"Compression force regulates cementoblast mineralization via S1PR1\/mitophagy axis","volume":"39","author":"Wang","year":"2025","journal-title":"FASEB J"}],"container-title":["Journal of Oral Biosciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1349007925000945?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1349007925000945?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,1,14]],"date-time":"2026-01-14T15:26:55Z","timestamp":1768404415000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S1349007925000945"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,12]]},"references-count":50,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2025,12]]}},"alternative-id":["S1349007925000945"],"URL":"https:\/\/doi.org\/10.1016\/j.job.2025.100705","relation":{},"ISSN":["1349-0079"],"issn-type":[{"value":"1349-0079","type":"print"}],"subject":[],"published":{"date-parts":[[2025,12]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Piezo1 negatively regulates proliferation, but enhances mineralization in human cementoblasts","name":"articletitle","label":"Article Title"},{"value":"Journal of Oral Biosciences","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.job.2025.100705","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2025 Japanese Association for Oral Biology. Published by Elsevier B.V. All rights are reserved, including those for text and data mining, AI training, and similar technologies.","name":"copyright","label":"Copyright"}],"article-number":"100705"}}