{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T16:21:37Z","timestamp":1792081297875,"version":"4.4.0"},"reference-count":42,"publisher":"Elsevier BV","issue":"10","license":[{"start":{"date-parts":[[2016,10,1]],"date-time":"2016-10-01T00:00:00Z","timestamp":1475280000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2016,10,1]],"date-time":"2016-10-01T00:00:00Z","timestamp":1475280000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2017,10,1]],"date-time":"2017-10-01T00:00:00Z","timestamp":1506816000000},"content-version":"vor","delay-in-days":365,"URL":"http:\/\/www.elsevier.com\/open-access\/userlicense\/1.0\/"}],"funder":[{"name":"China Health Ministry Program","award":["201302007"],"award-info":[{"award-number":["201302007"]}]},{"name":"NIH\/NIA OAIC","award":["P30-AG-028716"],"award-info":[{"award-number":["P30-AG-028716"]}]}],"content-domain":{"domain":["clinicalkey.com","clinicalkey.com.au","clinicalkey.es","clinicalkey.fr","clinicalkey.jp","oarsijournal.com","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Osteoarthritis and Cartilage"],"published-print":{"date-parts":[[2016,10]]},"DOI":"10.1016\/j.joca.2016.05.008","type":"journal-article","created":{"date-parts":[[2016,5,22]],"date-time":"2016-05-22T16:30:31Z","timestamp":1463934631000},"page":"1769-1775","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":236,"title":["Both systemic and local lipopolysaccharide (LPS) burden are associated with knee OA severity and inflammation"],"prefix":"10.1016","volume":"24","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0456-8379","authenticated-orcid":false,"given":"Z.Y.","family":"Huang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"T.","family":"Stabler","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"F.X.","family":"Pei","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"V.B.","family":"Kraus","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.joca.2016.05.008_bib1","doi-asserted-by":"crossref","first-page":"7","DOI":"10.1016\/j.joca.2013.10.018","article-title":"Osteoarthritis\u2014a case for personalized health care?","volume":"22","author":"Karsdal","year":"2014","journal-title":"Osteoarthritis Cartilage"},{"key":"10.1016\/j.joca.2016.05.008_bib2","doi-asserted-by":"crossref","first-page":"2115","DOI":"10.1016\/S0140-6736(11)60243-2","article-title":"Osteoarthritis: an update with relevance for clinical practice","volume":"377","author":"Bijlsma","year":"2011","journal-title":"Lancet"},{"key":"10.1016\/j.joca.2016.05.008_bib3","doi-asserted-by":"crossref","first-page":"123","DOI":"10.1038\/nrrheum.2015.158","article-title":"Does lipopolysaccharide-mediated inflammation have a role in OA?","volume":"12","author":"Huang","year":"2016","journal-title":"Nat Rev Rheumatol"},{"key":"10.1016\/j.joca.2016.05.008_bib4","doi-asserted-by":"crossref","first-page":"E740","DOI":"10.1152\/ajpendo.00302.2006","article-title":"Lipopolysaccharide activates an innate immune system response in human adipose tissue in obesity and type 2 diabetes","volume":"292","author":"Creely","year":"2007","journal-title":"Am J Physiol Endocrinol Metab"},{"key":"10.1016\/j.joca.2016.05.008_bib5","doi-asserted-by":"crossref","first-page":"G518","DOI":"10.1152\/ajpgi.00024.2006","article-title":"Increased intestinal permeability in obese mice: new evidence in the pathogenesis of nonalcoholic steatohepatitis","volume":"292","author":"Brun","year":"2007","journal-title":"Am J Physiol Gastrointest Liver Physiol"},{"key":"10.1016\/j.joca.2016.05.008_bib6","doi-asserted-by":"crossref","first-page":"15","DOI":"10.1186\/1476-9255-7-15","article-title":"Elevated endotoxin levels in non-alcoholic fatty liver disease","volume":"7","author":"Harte","year":"2010","journal-title":"J\u00a0Inflamm (Lond)"},{"key":"10.1016\/j.joca.2016.05.008_bib7","doi-asserted-by":"crossref","first-page":"1513","DOI":"10.1136\/gutjnl-2014-306928","article-title":"Microbiota and diabetes: an evolving relationship","volume":"63","author":"Tilg","year":"2014","journal-title":"Gut"},{"key":"10.1016\/j.joca.2016.05.008_bib8","doi-asserted-by":"crossref","first-page":"A263","DOI":"10.1016\/j.joca.2015.02.481","article-title":"Attenuation of hyaluronan fragment induced inflammatory response in macrophages by chondroitin sulphate","volume":"23","author":"Stabler","year":"2015","journal-title":"Osteoarthritis Cartilage"},{"key":"10.1016\/j.joca.2016.05.008_bib9","doi-asserted-by":"crossref","first-page":"849","DOI":"10.1136\/jcp.44.10.849","article-title":"Quantitative Limulus lysate assay for endotoxin and the effect of plasma","volume":"44","author":"Hurley","year":"1991","journal-title":"J\u00a0Clin Pathol"},{"key":"10.1016\/j.joca.2016.05.008_bib10","doi-asserted-by":"crossref","first-page":"42","DOI":"10.1006\/abio.1998.2655","article-title":"Proteinase K digestion of proteins improves detection of bacterial endotoxins by the Limulus amebocyte lysate assay: application for endotoxin removal from cationic proteins","volume":"259","author":"Petsch","year":"1998","journal-title":"Anal Biochem"},{"key":"10.1016\/j.joca.2016.05.008_bib11","doi-asserted-by":"crossref","first-page":"417","DOI":"10.1016\/S0171-2985(11)80354-2","article-title":"The bactericidal\/permeability-increasing protein (BPI), a potent element in host-defense against gram-negative bacteria and lipopolysaccharide","volume":"187","author":"Elsbach","year":"1993","journal-title":"Immunobiology"},{"issue":"9","key":"10.1016\/j.joca.2016.05.008_bib12","doi-asserted-by":"crossref","first-page":"1613","DOI":"10.1016\/j.joca.2016.04.010","article-title":"Direct in\u00a0vivo evidence of activated macrophages in human osteoarthritis (NCT01237405)","volume":"24","author":"Kraus","year":"2016","journal-title":"Osteoarthritis Cartilage"},{"key":"10.1016\/j.joca.2016.05.008_bib13","doi-asserted-by":"crossref","first-page":"494","DOI":"10.1136\/ard.16.4.494","article-title":"Radiological assessment of osteo-arthrosis","volume":"16","author":"Kellgren","year":"1957","journal-title":"Ann Rheum Dis"},{"key":"10.1016\/j.joca.2016.05.008_bib14","doi-asserted-by":"crossref","first-page":"956","DOI":"10.1002\/art.39006","article-title":"Soluble macrophage biomarkers indicate inflammatory phenotypes in patients with knee osteoarthritis","volume":"67","author":"Daghestani","year":"2015","journal-title":"Arthritis Rheumatol"},{"key":"10.1016\/j.joca.2016.05.008_bib15","doi-asserted-by":"crossref","first-page":"128","DOI":"10.1007\/s00256-002-0603-z","article-title":"Comparison of fixed-flexion positioning with fluoroscopic semi-flexed positioning for quantifying radiographic joint-space width in the knee: test-retest reproducibility","volume":"32","author":"Peterfy","year":"2003","journal-title":"Skelet Radiol"},{"issue":"Suppl A","key":"10.1016\/j.joca.2016.05.008_bib16","doi-asserted-by":"crossref","first-page":"A1","DOI":"10.1016\/j.joca.2006.11.009","article-title":"Atlas of individual radiographic features in osteoarthritis, revised","volume":"15","author":"Altman","year":"2007","journal-title":"Osteoarthritis Cartilage"},{"key":"10.1016\/j.joca.2016.05.008_bib17","first-page":"1833","article-title":"Validation study of WOMAC: a health status instrument for measuring clinically important patient relevant outcomes to antirheumatic drug therapy in patients with osteoarthritis of the hip or knee","volume":"15","author":"Bellamy","year":"1988","journal-title":"J\u00a0Rheumatol"},{"key":"10.1016\/j.joca.2016.05.008_bib18","doi-asserted-by":"crossref","first-page":"36","DOI":"10.1038\/nrmicro1068","article-title":"LPS, TLR4 and infectious disease diversity","volume":"3","author":"Miller","year":"2005","journal-title":"Nat Rev Microbiol"},{"key":"10.1016\/j.joca.2016.05.008_bib19","doi-asserted-by":"crossref","first-page":"R111","DOI":"10.1186\/ar4291","article-title":"Bacterial lipopolysaccharides form procollagen-endotoxin complexes that trigger cartilage inflammation and degeneration: implications for the development of rheumatoid arthritis","volume":"15","author":"Lorenz","year":"2013","journal-title":"Arthritis Res Ther"},{"key":"10.1016\/j.joca.2016.05.008_bib20","doi-asserted-by":"crossref","first-page":"273","DOI":"10.1155\/S0962935192000425","article-title":"Bacterial lipopolysaccharide potentiates type II collagen-induced arthritis in mice","volume":"1","author":"Caccese","year":"1992","journal-title":"Mediat Inflamm"},{"key":"10.1016\/j.joca.2016.05.008_bib21","doi-asserted-by":"crossref","first-page":"897","DOI":"10.1136\/gut.36.6.897","article-title":"Significance of systemic endotoxemia in inflammatory bowel disease","volume":"36","author":"Gardiner","year":"1995","journal-title":"Gut"},{"key":"10.1016\/j.joca.2016.05.008_bib22","doi-asserted-by":"crossref","first-page":"320","DOI":"10.1111\/j.1365-2796.2010.02270.x","article-title":"Effects of gut microbiota on obesity and atherosclerosis via modulation of inflammation and lipid metabolism","volume":"268","author":"Caesar","year":"2010","journal-title":"J\u00a0Intern Med"},{"key":"10.1016\/j.joca.2016.05.008_bib23","doi-asserted-by":"crossref","first-page":"15","DOI":"10.1016\/0014-4827(82)90150-1","article-title":"Endotoxin-induced degranulation of the Limulus amebocyte","volume":"140","author":"Armstrong","year":"1982","journal-title":"Exp Cell Res"},{"key":"10.1016\/j.joca.2016.05.008_bib24","doi-asserted-by":"crossref","first-page":"149","DOI":"10.1016\/0167-4838(93)90076-4","article-title":"Two forms of factor C from the amoebocytes of Carcinoscorpius rotundicauda: purification and characterisation","volume":"1202","author":"Ding","year":"1993","journal-title":"Biochim Biophys Acta"},{"key":"10.1016\/j.joca.2016.05.008_bib25","first-page":"90","article-title":"Molecular cloning and sequence analysis of factor C cDNA from the Singapore horseshoe crab, Carcinoscorpius rotundicauda","volume":"4","author":"Ding","year":"1995","journal-title":"Mol Mar Biol Biotechnol"},{"key":"10.1016\/j.joca.2016.05.008_bib26","doi-asserted-by":"crossref","first-page":"277","DOI":"10.1016\/S0167-7799(01)01694-8","article-title":"A\u00a0new era in pyrogen testing","volume":"19","author":"Ding","year":"2001","journal-title":"Trends Biotechnol"},{"key":"10.1016\/j.joca.2016.05.008_bib27","doi-asserted-by":"crossref","first-page":"4988","DOI":"10.1128\/AEM.00527-10","article-title":"Evaluation of the Limulus amebocyte lysate and recombinant factor C assays for assessment of airborne endotoxin","volume":"76","author":"Thorne","year":"2010","journal-title":"Appl Environ Microbiol"},{"key":"10.1016\/j.joca.2016.05.008_bib28","first-page":"153","article-title":"Optimization of detection of bacterial endotoxin in plasma with the Limulus test","volume":"116","author":"Roth","year":"1990","journal-title":"J\u00a0Lab Clin Med"},{"key":"10.1016\/j.joca.2016.05.008_bib29","doi-asserted-by":"crossref","first-page":"192","DOI":"10.1007\/BF01641194","article-title":"Quantitation of endotoxin in blood from patients with meningococcal disease using a Limulus lysate test in combination with chromogenic substrate","volume":"11","author":"Harthug","year":"1983","journal-title":"Infection"},{"key":"10.1016\/j.joca.2016.05.008_bib30","doi-asserted-by":"crossref","first-page":"e41258","DOI":"10.1371\/journal.pone.0041258","article-title":"Detection of microbial translocation in HIV and SIV infection using the Limulus amebocyte lysate assay is masked by serum and plasma","volume":"7","author":"Balagopal","year":"2012","journal-title":"PLoS One"},{"key":"10.1016\/j.joca.2016.05.008_bib31","doi-asserted-by":"crossref","first-page":"392","DOI":"10.2337\/dc10-1676","article-title":"Endotoxemia is associated with an increased risk of incident diabetes","volume":"34","author":"Pussinen","year":"2011","journal-title":"Diabetes Care"},{"key":"10.1016\/j.joca.2016.05.008_bib32","doi-asserted-by":"crossref","first-page":"1761","DOI":"10.2337\/db06-1491","article-title":"Metabolic endotoxemia initiates obesity and insulin resistance","volume":"56","author":"Cani","year":"2007","journal-title":"Diabetes"},{"key":"10.1016\/j.joca.2016.05.008_bib33","doi-asserted-by":"crossref","first-page":"1470","DOI":"10.2337\/db07-1403","article-title":"Changes in gut microbiota control metabolic endotoxemia-induced inflammation in high-fat diet-induced obesity and diabetes in mice","volume":"57","author":"Cani","year":"2008","journal-title":"Diabetes"},{"key":"10.1016\/j.joca.2016.05.008_bib34","doi-asserted-by":"crossref","first-page":"1091","DOI":"10.1136\/gut.2008.165886","article-title":"Changes in gut microbiota control inflammation in obese mice through a mechanism involving GLP-2-driven improvement of gut permeability","volume":"58","author":"Cani","year":"2009","journal-title":"Gut"},{"key":"10.1016\/j.joca.2016.05.008_bib35","doi-asserted-by":"crossref","first-page":"2416","DOI":"10.1096\/fj.07-102723","article-title":"Gut microbiota modulation with norfloxacin and ampicillin enhances glucose tolerance in mice","volume":"22","author":"Membrez","year":"2008","journal-title":"FASEB J"},{"key":"10.1016\/j.joca.2016.05.008_bib36","doi-asserted-by":"crossref","first-page":"494","DOI":"10.1016\/j.atherosclerosis.2008.06.018","article-title":"Ethnic and sex differences in circulating endotoxin levels: a novel marker of atherosclerotic and cardiovascular risk in a British multi-ethnic population","volume":"203","author":"Miller","year":"2009","journal-title":"Atherosclerosis"},{"key":"10.1016\/j.joca.2016.05.008_bib37","first-page":"903","article-title":"Detection of endotoxin in human blood and demonstration of an inhibitor","volume":"75","author":"Levin","year":"1970","journal-title":"J\u00a0Lab Clin Med"},{"key":"10.1016\/j.joca.2016.05.008_bib38","doi-asserted-by":"crossref","first-page":"989","DOI":"10.1042\/BST0390989","article-title":"Old and new findings on lipopolysaccharide-binding protein: a soluble pattern-recognition molecule","volume":"39","author":"Schumann","year":"2011","journal-title":"Biochem Soc Trans"},{"key":"10.1016\/j.joca.2016.05.008_bib39","doi-asserted-by":"crossref","first-page":"10867","DOI":"10.1016\/S0021-9258(18)81700-8","article-title":"Identification of a lipid A binding site in the acute phase reactant lipopolysaccharide binding protein","volume":"264","author":"Tobias","year":"1989","journal-title":"J\u00a0Biol Chem"},{"key":"10.1016\/j.joca.2016.05.008_bib40","doi-asserted-by":"crossref","first-page":"25","DOI":"10.1016\/j.jacc.2007.02.070","article-title":"Association of lipopolysaccharide-binding protein and coronary artery disease in men","volume":"50","author":"Lepper","year":"2007","journal-title":"J\u00a0Am Coll Cardiol"},{"key":"10.1016\/j.joca.2016.05.008_bib41","doi-asserted-by":"crossref","first-page":"208","DOI":"10.1053\/jhep.2003.50038","article-title":"Increased lipopolysaccharide binding protein in cirrhotic patients with marked immune and hemodynamic derangement","volume":"37","author":"Albillos","year":"2003","journal-title":"Hepatology"},{"key":"10.1016\/j.joca.2016.05.008_bib42","doi-asserted-by":"crossref","first-page":"124","DOI":"10.1186\/1479-5876-10-124","article-title":"A\u00a0human model of inflammatory cardio-metabolic dysfunction; a double blind placebo-controlled crossover trial","volume":"10","author":"Mehta","year":"2012","journal-title":"J\u00a0Transl Med"}],"container-title":["Osteoarthritis and Cartilage"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1063458416300863?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1063458416300863?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,10,21]],"date-time":"2025-10-21T03:43:31Z","timestamp":1761018211000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S1063458416300863"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016,10]]},"references-count":42,"journal-issue":{"issue":"10","published-print":{"date-parts":[[2016,10]]}},"alternative-id":["S1063458416300863"],"URL":"https:\/\/doi.org\/10.1016\/j.joca.2016.05.008","relation":{},"ISSN":["1063-4584"],"issn-type":[{"value":"1063-4584","type":"print"}],"subject":[],"published":{"date-parts":[[2016,10]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Both systemic and local lipopolysaccharide (LPS) burden are associated with knee OA severity and inflammation","name":"articletitle","label":"Article Title"},{"value":"Osteoarthritis and Cartilage","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.joca.2016.05.008","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2016 Osteoarthritis Research Society International. Published by Elsevier Ltd.","name":"copyright","label":"Copyright"}]}}