{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,5]],"date-time":"2026-06-05T16:49:56Z","timestamp":1780678196403,"version":"3.54.1"},"reference-count":49,"publisher":"Elsevier BV","issue":"12","license":[{"start":{"date-parts":[[2016,12,1]],"date-time":"2016-12-01T00:00:00Z","timestamp":1480550400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2016,12,1]],"date-time":"2016-12-01T00:00:00Z","timestamp":1480550400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2017,12,1]],"date-time":"2017-12-01T00:00:00Z","timestamp":1512086400000},"content-version":"vor","delay-in-days":365,"URL":"http:\/\/www.elsevier.com\/open-access\/userlicense\/1.0\/"}],"funder":[{"name":"German Department of Defence","award":["GZE\/U2AD\/CD524\/DF560"],"award-info":[{"award-number":["GZE\/U2AD\/CD524\/DF560"]}]}],"content-domain":{"domain":["clinicalkey.com","clinicalkey.com.au","clinicalkey.es","clinicalkey.fr","clinicalkey.jp","oarsijournal.com","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Osteoarthritis and Cartilage"],"published-print":{"date-parts":[[2016,12]]},"DOI":"10.1016\/j.joca.2016.07.019","type":"journal-article","created":{"date-parts":[[2016,8,8]],"date-time":"2016-08-08T02:17:50Z","timestamp":1470622670000},"page":"2171-2180","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":52,"title":["Antioxidative therapy in an ex\u00a0vivo human cartilage trauma-model: attenuation of trauma-induced cell loss and ECM-destructive enzymes by N-acetyl cysteine"],"prefix":"10.1016","volume":"24","author":[{"given":"J.","family":"Riegger","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H.","family":"Joos","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H.G.","family":"Palm","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"B.","family":"Friemert","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H.","family":"Reichel","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A.","family":"Ignatius","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R.E.","family":"Brenner","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.joca.2016.07.019_bib1","doi-asserted-by":"crossref","first-page":"1145","DOI":"10.1016\/j.joca.2013.03.018","article-title":"The epidemiology and impact of pain in osteoarthritis","volume":"21","author":"Neogi","year":"2013","journal-title":"Osteoarthritis Cartilage"},{"key":"10.1016\/j.joca.2016.07.019_bib2","doi-asserted-by":"crossref","first-page":"99","DOI":"10.1007\/s12178-011-9088-5","article-title":"Risk factors for incident osteoarthritis of the hip and knee","volume":"4","author":"Chaganti","year":"2011","journal-title":"Curr Rev Musculoskelet Med"},{"key":"10.1016\/j.joca.2016.07.019_bib3","doi-asserted-by":"crossref","first-page":"S135","DOI":"10.1097\/00003086-199910001-00015","article-title":"Biologic repair of articular cartilage. Defect models in experimental animals and matrix requirements","author":"Hunziker","year":"1999","journal-title":"Clin Orthop Relat Res"},{"key":"10.1016\/j.joca.2016.07.019_bib4","doi-asserted-by":"crossref","first-page":"726","DOI":"10.7326\/0003-4819-133-9-200011070-00015","article-title":"Osteoarthritis: new insights. Part 2: treatment approaches","volume":"133","author":"Felson","year":"2000","journal-title":"Ann Intern Med"},{"key":"10.1016\/j.joca.2016.07.019_bib5","doi-asserted-by":"crossref","first-page":"286","DOI":"10.1177\/1947603513495889","article-title":"The roles of mechanical stresses in the pathogenesis of osteoarthritis: implications for treatment of joint injuries","volume":"4","author":"Buckwalter","year":"2013","journal-title":"Cartilage"},{"key":"10.1016\/j.joca.2016.07.019_bib6","first-page":"271","article-title":"Single impact trauma in human early-stage osteoarthritic cartilage: implication of prostaglandin D2 but no additive effect of IL-1beta on cell survival","volume":"28","author":"Joos","year":"2011","journal-title":"Int J Mol Med"},{"key":"10.1016\/j.joca.2016.07.019_bib7","doi-asserted-by":"crossref","first-page":"1244","DOI":"10.1016\/j.joca.2009.03.007","article-title":"Anti-apoptotic treatments prevent cartilage degradation after acute trauma to human ankle cartilage","volume":"17","author":"Pascual Garrido","year":"2009","journal-title":"Osteoarthritis Cartilage"},{"key":"10.1016\/j.joca.2016.07.019_bib8","doi-asserted-by":"crossref","first-page":"703","DOI":"10.1016\/S0736-0266(00)00066-8","article-title":"Chondrocyte necrosis and apoptosis in impact damaged articular cartilage","volume":"19","author":"Chen","year":"2001","journal-title":"J\u00a0Orthop Res"},{"key":"10.1016\/j.joca.2016.07.019_bib9","doi-asserted-by":"crossref","DOI":"10.1186\/ar4591","article-title":"Targeting pro-inflammatory cytokines following joint injury: acute intra-articular inhibition of interleukin-1 following knee injury prevents post-traumatic arthritis","volume":"16","author":"Furman","year":"2014","journal-title":"Arthritis Res Ther"},{"key":"10.1016\/j.joca.2016.07.019_bib10","doi-asserted-by":"crossref","first-page":"15703","DOI":"10.1074\/jbc.271.26.15703","article-title":"Reactive oxygen species mediate cytokine activation of c-jun NH2-terminal kinases","volume":"271","author":"Lo","year":"1996","journal-title":"J\u00a0Biol Chem"},{"key":"10.1016\/j.joca.2016.07.019_bib11","doi-asserted-by":"crossref","first-page":"270","DOI":"10.1038\/sj.bjp.0703787","article-title":"N-acetylcysteine attenuates TNF-alpha-induced p38 MAP kinase activation and p38 MAP kinase-mediated IL-8 production by human pulmonary vascular endothelial cells","volume":"132","author":"Hashimoto","year":"2001","journal-title":"Br J Pharmacol"},{"key":"10.1016\/j.joca.2016.07.019_bib12","doi-asserted-by":"crossref","first-page":"593","DOI":"10.1016\/0891-5849(89)90066-X","article-title":"The antioxidant action of N-acetylcysteine \u2013 its reaction with hydrogen-peroxide, hydroxyl radical, superoxide, and hypochlorous acid","volume":"6","author":"Aruoma","year":"1989","journal-title":"Free Radic Bio Med"},{"key":"10.1016\/j.joca.2016.07.019_bib13","doi-asserted-by":"crossref","first-page":"133","DOI":"10.1016\/j.bbapap.2011.06.020","article-title":"Proteases involved in cartilage matrix degradation in osteoarthritis","volume":"1824","author":"Troeberg","year":"2012","journal-title":"Biochim Biophys Acta"},{"key":"10.1016\/j.joca.2016.07.019_bib14","doi-asserted-by":"crossref","first-page":"1044","DOI":"10.1089\/ars.2013.5634","article-title":"Glycosaminoglycan degradation by selected reactive oxygen species","volume":"21","author":"Fuchs","year":"2014","journal-title":"Antioxid Redox Signal"},{"key":"10.1016\/j.joca.2016.07.019_bib15","doi-asserted-by":"crossref","first-page":"156","DOI":"10.1002\/jor.20976","article-title":"N-acetylcysteine prevents nitric oxide-induced chondrocyte apoptosis and cartilage degeneration in an experimental model of osteoarthritis","volume":"28","author":"Nakagawa","year":"2010","journal-title":"J\u00a0Orthop Res"},{"key":"10.1016\/j.joca.2016.07.019_bib16","doi-asserted-by":"crossref","first-page":"21","DOI":"10.1111\/j.1541-4337.2004.tb00058.x","article-title":"Reactive oxygen species, aging, and antioxidative nutraceuticals","volume":"3","author":"Lee","year":"2004","journal-title":"Compr Rev Food Sci F"},{"key":"10.1016\/j.joca.2016.07.019_bib17","doi-asserted-by":"crossref","first-page":"125","DOI":"10.1016\/j.ejphar.2009.09.016","article-title":"Differential effects of the antioxidant n-acetylcysteine on the production of catabolic mediators in IL-1beta-stimulated human osteoarthritic synoviocytes and chondrocytes","volume":"623","author":"Roman-Blas","year":"2009","journal-title":"Eur J Pharmacol"},{"key":"10.1016\/j.joca.2016.07.019_bib18","doi-asserted-by":"crossref","first-page":"1634","DOI":"10.1016\/S0022-3565(24)38315-6","article-title":"Induction of stromelysin gene expression by tumor necrosis factor alpha is inhibited by dexamethasone, salicylate, and N-acetylcysteine in synovial fibroblasts","volume":"289","author":"Morin","year":"1999","journal-title":"J\u00a0Pharmacol Exp Ther"},{"key":"10.1016\/j.joca.2016.07.019_bib19","doi-asserted-by":"crossref","first-page":"958","DOI":"10.1016\/j.joca.2005.06.008","article-title":"Altered cartilage mechanics and histology in knee osteoarthritis: relation to clinical assessment (ICRS Grade)","volume":"13","author":"Kleemann","year":"2005","journal-title":"Osteoarthritis Cartilage"},{"key":"10.1016\/j.joca.2016.07.019_bib20","doi-asserted-by":"crossref","first-page":"103","DOI":"10.1016\/0003-2697(87)90658-0","article-title":"Microdetermination of proteoglycans and glycosaminoglycans in the presence of guanidine hydrochloride","volume":"161","author":"Chandrasekhar","year":"1987","journal-title":"Anal Biochem"},{"key":"10.1016\/j.joca.2016.07.019_bib21","doi-asserted-by":"crossref","first-page":"756","DOI":"10.1016\/j.joca.2007.10.009","article-title":"Interleukin-1beta and interleukin-6 disturb the antioxidant enzyme system in bovine chondrocytes: a possible explanation for oxidative stress generation","volume":"16","author":"Mathy-Hartert","year":"2008","journal-title":"Osteoarthritis Cartilage"},{"key":"10.1016\/j.joca.2016.07.019_bib22","doi-asserted-by":"crossref","first-page":"643","DOI":"10.1016\/j.joca.2005.04.002","article-title":"Oxygen and reactive oxygen species in cartilage degradation: friends or foes?","volume":"13","author":"Henrotin","year":"2005","journal-title":"Osteoarthritis Cartilage"},{"key":"10.1016\/j.joca.2016.07.019_bib23","doi-asserted-by":"crossref","first-page":"3465","DOI":"10.1074\/jbc.272.6.3465","article-title":"Metabolic fate of peroxynitrite in aqueous solution. Reaction with nitric oxide and pH-dependent decomposition to nitrite and oxygen in a 2:1 stoichiometry","volume":"272","author":"Pfeiffer","year":"1997","journal-title":"J\u00a0Biol Chem"},{"key":"10.1016\/j.joca.2016.07.019_bib24","doi-asserted-by":"crossref","first-page":"2349","DOI":"10.1002\/art.10496","article-title":"Detection of nitrotyrosine in aging and osteoarthritic cartilage: correlation of oxidative damage with the presence of interleukin-1beta and with chondrocyte resistance to insulin-like growth factor 1","volume":"46","author":"Loeser","year":"2002","journal-title":"Arthritis Rheum"},{"key":"10.1016\/j.joca.2016.07.019_bib25","doi-asserted-by":"crossref","first-page":"13S","DOI":"10.1177\/1947603513487457","article-title":"Key pathways to prevent posttraumatic arthritis for future molecule-based therapy","volume":"4","author":"Chubinskaya","year":"2013","journal-title":"Cartilage"},{"key":"10.1016\/j.joca.2016.07.019_bib26","doi-asserted-by":"crossref","first-page":"1890","DOI":"10.2106\/JBJS.H.00545","article-title":"N-acetylcysteine inhibits post-impact chondrocyte death in osteochondral explants","volume":"91","author":"Martin","year":"2009","journal-title":"J\u00a0Bone Jt Surg Am"},{"key":"10.1016\/j.joca.2016.07.019_bib27","doi-asserted-by":"crossref","first-page":"549","DOI":"10.1016\/S0008-6363(99)00396-X","article-title":"Markers of apoptosis in cardiovascular tissues: focus on Annexin V","volume":"45","author":"van Heerde","year":"2000","journal-title":"Cardiovasc Res"},{"key":"10.1016\/j.joca.2016.07.019_bib28","doi-asserted-by":"crossref","first-page":"858","DOI":"10.1091\/mbc.e12-10-0747","article-title":"Regulation of apoptosis by Bcl-2 cysteine oxidation in human lung epithelial cells","volume":"24","author":"Luanpitpong","year":"2013","journal-title":"Mol Biol Cell"},{"key":"10.1016\/j.joca.2016.07.019_bib29","doi-asserted-by":"crossref","first-page":"1662","DOI":"10.1016\/j.bcp.2010.08.013","article-title":"Involvement of ROS in chlorogenic acid-induced apoptosis of Bcr-Abl+ CML cells","volume":"80","author":"Rakshit","year":"2010","journal-title":"Biochem Pharmacol"},{"key":"10.1016\/j.joca.2016.07.019_bib30","doi-asserted-by":"crossref","first-page":"73","DOI":"10.1124\/jpet.106.114678","article-title":"Inhibition of acrolein-induced apoptosis by the antioxidant N-acetylcysteine","volume":"321","author":"Tanel","year":"2007","journal-title":"J\u00a0Pharmacol Exp Ther"},{"key":"10.1016\/j.joca.2016.07.019_bib31","doi-asserted-by":"crossref","first-page":"1343","DOI":"10.1089\/ars.2007.1957","article-title":"Redox regulation of cell survival","volume":"10","author":"Trachootham","year":"2008","journal-title":"Antioxid Redox Sign"},{"key":"10.1016\/j.joca.2016.07.019_bib32","doi-asserted-by":"crossref","first-page":"137","DOI":"10.1080\/07315724.1986.10720121","article-title":"Intracellular cysteine and glutathione delivery systems","volume":"5","author":"Meister","year":"1986","journal-title":"J\u00a0Am Coll Nutr"},{"key":"10.1016\/j.joca.2016.07.019_bib33","doi-asserted-by":"crossref","first-page":"614","DOI":"10.1016\/j.joca.2005.02.011","article-title":"Vulnerability to ROS-induced cell death in ageing articular cartilage: the role of antioxidant enzyme activity","volume":"13","author":"Jallali","year":"2005","journal-title":"Osteoarthritis Cartilage"},{"key":"10.1016\/j.joca.2016.07.019_bib34","doi-asserted-by":"crossref","first-page":"324","DOI":"10.1016\/j.stem.2009.01.015","article-title":"Migratory chondrogenic progenitor cells from repair tissue during the later stages of human osteoarthritis","volume":"4","author":"Koelling","year":"2009","journal-title":"Cell Stem Cell"},{"key":"10.1016\/j.joca.2016.07.019_bib35","doi-asserted-by":"crossref","first-page":"469","DOI":"10.1016\/j.cellsig.2003.09.008","article-title":"Interleukin-17 signal transduction pathways implicated in inducing matrix metalloproteinase-3, -13 and aggrecanase-1 genes in articular chondrocytes","volume":"16","author":"Sylvester","year":"2004","journal-title":"Cell Signal"},{"key":"10.1016\/j.joca.2016.07.019_bib36","doi-asserted-by":"crossref","first-page":"19","DOI":"10.1002\/(SICI)1097-4644(19980401)69:1<19::AID-JCB3>3.0.CO;2-Y","article-title":"Interleukin-1 beta induction of c-fos and collagenase expression in articular chondrocytes: involvement of reactive oxygen species","volume":"69","author":"Lo","year":"1998","journal-title":"J\u00a0Cell Biochem"},{"key":"10.1016\/j.joca.2016.07.019_bib37","doi-asserted-by":"crossref","first-page":"489","DOI":"10.1002\/art.23120","volume":"58","author":"Stevens","year":"2008","journal-title":"Arthritis Rheum"},{"key":"10.1016\/j.joca.2016.07.019_bib38","doi-asserted-by":"crossref","first-page":"59","DOI":"10.1023\/A:1006828402225","article-title":"Hydrogen peroxide-induced expression of the proto-oncogenes, c-jun, c-fos and c-myc in rabbit lens epithelial cells","volume":"173","author":"Li","year":"1997","journal-title":"Mol Cell Biochem"},{"key":"10.1016\/j.joca.2016.07.019_bib39","doi-asserted-by":"crossref","first-page":"2247","DOI":"10.1002\/j.1460-2075.1991.tb07761.x","article-title":"Reactive oxygen intermediates as apparently widely used messengers in the activation of the NF-kappa-B transcription factor and HIV-1","volume":"10","author":"Schreck","year":"1991","journal-title":"EMBO J"},{"key":"10.1016\/j.joca.2016.07.019_bib40","doi-asserted-by":"crossref","first-page":"26","DOI":"10.1046\/j.1365-2249.1999.00749.x","article-title":"Effects of bucillamine and N-acetyl-L-cysteine on cytokine production and collagen-induced arthritis (CIA)","volume":"115","author":"Tsuji","year":"1999","journal-title":"Clin Exp Immunol"},{"key":"10.1016\/j.joca.2016.07.019_bib41","doi-asserted-by":"crossref","first-page":"208","DOI":"10.1016\/S0014-4827(03)00180-0","article-title":"Induction of matrix metalloproteinase-13 gene expression by TNF-alpha is mediated by MAP kinases, AP-1, and NF-kappa B transcription factors in articular chondrocytes","volume":"288","author":"Liacini","year":"2003","journal-title":"Exp Cell Res"},{"key":"10.1016\/j.joca.2016.07.019_bib42","first-page":"139","article-title":"The regulation of the ADAMTS4 and ADAMTS5 aggrecanases in osteoarthritis: a review","volume":"26","author":"Bondeson","year":"2008","journal-title":"Clin Exp Rheumatol"},{"key":"10.1016\/j.joca.2016.07.019_bib43","doi-asserted-by":"crossref","first-page":"808","DOI":"10.1002\/art.21638","article-title":"Comparative analysis of gene, expression profiles in intact and damaged regions of human osteoarthritic cartilage","volume":"54","author":"Sato","year":"2006","journal-title":"Arthritis Rheum"},{"key":"10.1016\/j.joca.2016.07.019_bib44","doi-asserted-by":"crossref","first-page":"371426","DOI":"10.1155\/2014\/371426","article-title":"Progression of gene expression changes following a mechanical injury to articular cartilage as a model of early stage osteoarthritis","volume":"2014","author":"McCulloch","year":"2014","journal-title":"Arthritis"},{"key":"10.1016\/j.joca.2016.07.019_bib45","doi-asserted-by":"crossref","first-page":"112","DOI":"10.1053\/joca.2000.0366","article-title":"Quantitative analysis of gene expression in human articular cartilage from normal and osteoarthritic joints","volume":"9","author":"Martin","year":"2001","journal-title":"Osteoarthritis Cartilage"},{"key":"10.1016\/j.joca.2016.07.019_bib46","doi-asserted-by":"crossref","first-page":"235","DOI":"10.1016\/S1063-4584(05)80075-5","article-title":"Studies on type II collagen and aggrecan production in human articular chondrocytes in\u00a0vitro and effects of transforming growth factor-beta and interleukin-1beta","volume":"2","author":"Frazer","year":"1994","journal-title":"Osteoarthr Cartilage"},{"key":"10.1016\/j.joca.2016.07.019_bib47","doi-asserted-by":"crossref","first-page":"160","DOI":"10.1089\/ten.tec.2013.0056","article-title":"Extracellular matrix domain formation as an indicator of chondrocyte dedifferentiation and hypertrophy","volume":"20","author":"Wu","year":"2014","journal-title":"Tissue Eng Part C Methods"},{"key":"10.1016\/j.joca.2016.07.019_bib48","doi-asserted-by":"crossref","first-page":"1315","DOI":"10.1016\/j.freeradbiomed.2006.07.014","article-title":"Reduced nonprotein thiols inhibit activation and function of MMP-9: implications for chemoprevention","volume":"41","author":"Pei","year":"2006","journal-title":"Free Radic Biol Med"},{"key":"10.1016\/j.joca.2016.07.019_bib49","doi-asserted-by":"crossref","first-page":"467","DOI":"10.1007\/s000110050226","article-title":"Agents that block fibronectin fragment-mediated cartilage damage also promote repair","volume":"46","author":"Homandberg","year":"1997","journal-title":"Inflamm Res"}],"container-title":["Osteoarthritis and Cartilage"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1063458416302114?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1063458416302114?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,10,21]],"date-time":"2025-10-21T03:44:17Z","timestamp":1761018257000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S1063458416302114"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016,12]]},"references-count":49,"journal-issue":{"issue":"12","published-print":{"date-parts":[[2016,12]]}},"alternative-id":["S1063458416302114"],"URL":"https:\/\/doi.org\/10.1016\/j.joca.2016.07.019","relation":{},"ISSN":["1063-4584"],"issn-type":[{"value":"1063-4584","type":"print"}],"subject":[],"published":{"date-parts":[[2016,12]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Antioxidative therapy in an ex\u00a0vivo human cartilage trauma-model: attenuation of trauma-induced cell loss and ECM-destructive enzymes by N-acetyl cysteine","name":"articletitle","label":"Article Title"},{"value":"Osteoarthritis and Cartilage","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.joca.2016.07.019","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2016 Osteoarthritis Research Society International. Published by Elsevier Ltd. All rights reserved.","name":"copyright","label":"Copyright"}]}}