{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,24]],"date-time":"2026-10-24T02:12:02Z","timestamp":1792807922221,"version":"4.5.0"},"reference-count":41,"publisher":"Elsevier BV","issue":"1","license":[{"start":{"date-parts":[[2023,1,1]],"date-time":"2023-01-01T00:00:00Z","timestamp":1672531200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2023,1,1]],"date-time":"2023-01-01T00:00:00Z","timestamp":1672531200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2024,1,1]],"date-time":"2024-01-01T00:00:00Z","timestamp":1704067200000},"content-version":"vor","delay-in-days":365,"URL":"http:\/\/www.elsevier.com\/open-access\/userlicense\/1.0\/"}],"content-domain":{"domain":["clinicalkey.com","clinicalkey.com.au","clinicalkey.es","clinicalkey.fr","clinicalkey.jp","oarsijournal.com","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Osteoarthritis and Cartilage"],"published-print":{"date-parts":[[2023,1]]},"DOI":"10.1016\/j.joca.2022.09.011","type":"journal-article","created":{"date-parts":[[2022,10,7]],"date-time":"2022-10-07T02:19:34Z","timestamp":1665109174000},"page":"72-82","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":23,"title":["Phenotypic and functional characterisation of synovial fluid-derived neutrophils in knee osteoarthritis and knee infection"],"prefix":"10.1016","volume":"31","author":[{"given":"G.","family":"Manukyan","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7424-8653","authenticated-orcid":false,"given":"J.","family":"Gallo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5509-7562","authenticated-orcid":false,"given":"Z.","family":"Mikulkova","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9572-8613","authenticated-orcid":false,"given":"M.","family":"Trajerova","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3067-7867","authenticated-orcid":false,"given":"J.","family":"Savara","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Z.","family":"Slobodova","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"E.","family":"Fidler","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"B.","family":"Shrestha","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8969-4197","authenticated-orcid":false,"given":"E.","family":"Kriegova","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.joca.2022.09.011_bib1","doi-asserted-by":"crossref","first-page":"253","DOI":"10.1002\/jor.24457","article-title":"Role of inflammation and the immune system in the progression of osteoarthritis","volume":"38","author":"Woodell-May","year":"2020","journal-title":"J\u00a0Orthop Res"},{"key":"10.1016\/j.joca.2022.09.011_bib2","doi-asserted-by":"crossref","first-page":"207","DOI":"10.1016\/j.joca.2021.11.009","article-title":"Osteoarthritis year in review 2021: biology","volume":"30","author":"Jiang","year":"2022","journal-title":"Osteoarthr Cartil"},{"key":"10.1016\/j.joca.2022.09.011_bib3","doi-asserted-by":"crossref","first-page":"458","DOI":"10.1016\/j.joca.2015.09.013","article-title":"Synovitis and the risk of knee osteoarthritis: the MOST Study","volume":"24","author":"Felson","year":"2016","journal-title":"Osteoarthr Cartil"},{"issue":"4","key":"10.1016\/j.joca.2022.09.011_bib4","doi-asserted-by":"crossref","first-page":"501","DOI":"10.1016\/j.joca.2021.06.013","article-title":"Fundamentals of OA. An initiative of osteoarthritis and cartilage. Chapter 9: obesity and metabolic factors in OA","volume":"30","author":"Batushansky","year":"2022","journal-title":"Osteoarthr Cartil"},{"key":"10.1016\/j.joca.2022.09.011_bib5","doi-asserted-by":"crossref","first-page":"77","DOI":"10.1177\/1759720X12467868","article-title":"Role of inflammation in the pathogenesis of osteoarthritis: latest findings and interpretations","volume":"5","author":"Sokolove","year":"2013","journal-title":"Ther Adv Musculoskelet Dis"},{"key":"10.1016\/j.joca.2022.09.011_bib6","doi-asserted-by":"crossref","first-page":"37","DOI":"10.1016\/bs.acc.2020.04.002","article-title":"Pathophysiological landscape of osteoarthritis","volume":"100","author":"Kulkarni","year":"2021","journal-title":"Adv Clin Chem"},{"key":"10.1016\/j.joca.2022.09.011_bib7","doi-asserted-by":"crossref","first-page":"22","DOI":"10.1016\/j.joca.2014.10.002","article-title":"Metabolic triggered inflammation in osteoarthritis","volume":"23","author":"Wang","year":"2015","journal-title":"Osteoarthr Cartil"},{"key":"10.1016\/j.joca.2022.09.011_bib8","doi-asserted-by":"crossref","first-page":"7","DOI":"10.1016\/j.joca.2017.10.013","article-title":"Hierarchical, imbalanced pro-inflammatory cytokine networks govern the pathogenesis of chronic arthropathies","volume":"26","author":"Livshits","year":"2018","journal-title":"Osteoarthr Cartil"},{"issue":"2","key":"10.1016\/j.joca.2022.09.011_bib9","doi-asserted-by":"crossref","first-page":"468","DOI":"10.3390\/ijms19020468","article-title":"Neutrophils: beneficial and harmful cells in septic arthritis","volume":"19","author":"Boff","year":"2018","journal-title":"Int J Mol Sci"},{"key":"10.1016\/j.joca.2022.09.011_bib10","doi-asserted-by":"crossref","first-page":"89","DOI":"10.1002\/art.41486","article-title":"Synergistic roles of macrophages and neutrophils in osteoarthritis progression","volume":"73","author":"Hsueh","year":"2021","journal-title":"Arthritis Rheumatol"},{"key":"10.1016\/j.joca.2022.09.011_bib11","doi-asserted-by":"crossref","first-page":"1247","DOI":"10.1016\/j.joca.2018.04.016","article-title":"Gender-related differences observed among immune cells in synovial fluid in knee osteoarthritis","volume":"26","author":"Kriegova","year":"2018","journal-title":"Osteoarthr Cartil"},{"key":"10.1016\/j.joca.2022.09.011_bib12","article-title":"Neutrophil elastase induces chondrocyte apoptosis and facilitates the occurrence of osteoarthritis via caspase signaling pathway","volume":"12","author":"Wang","year":"2021","journal-title":"Front Pharmacol"},{"key":"10.1016\/j.joca.2022.09.011_bib13","doi-asserted-by":"crossref","first-page":"133","DOI":"10.1016\/j.bbapap.2011.06.020","article-title":"Proteases involved in cartilage matrix degradation in osteoarthritis","volume":"1824","author":"Troeberg","year":"2012","journal-title":"Biochim Biophys Acta"},{"key":"10.1016\/j.joca.2022.09.011_bib14","doi-asserted-by":"crossref","first-page":"1466","DOI":"10.1002\/art.34315","article-title":"Active involvement of alarmins S100A8 and S100A9 in the regulation of synovial activation and joint destruction during mouse and human osteoarthritis","volume":"64","author":"van Lent","year":"2012","journal-title":"Arthritis Rheum"},{"key":"10.1016\/j.joca.2022.09.011_bib15","doi-asserted-by":"crossref","first-page":"2857","DOI":"10.4049\/jimmunol.1301539","article-title":"Processes of sterile inflammation","volume":"191","author":"Shen","year":"2013","journal-title":"J\u00a0Immunol"},{"key":"10.1016\/j.joca.2022.09.011_bib16","article-title":"The damage-associated molecular patterns (DAMPs) as potential targets to treat osteoarthritis: perspectives from a review of the literature","volume":"7","author":"Lambert","year":"2020","journal-title":"Front Med (Lausanne)"},{"key":"10.1016\/j.joca.2022.09.011_bib17","doi-asserted-by":"crossref","first-page":"483","DOI":"10.1136\/ard.2009.113100","article-title":"EULAR evidence-based recommendations for the diagnosis of knee osteoarthritis","volume":"69","author":"Zhang","year":"2010","journal-title":"Ann Rheum Dis"},{"key":"10.1016\/j.joca.2022.09.011_bib18","doi-asserted-by":"crossref","first-page":"494","DOI":"10.1136\/ard.16.4.494","article-title":"Radiological assessment of osteo-arthrosis","volume":"16","author":"Kellgren","year":"1957","journal-title":"Ann Rheum Dis"},{"key":"10.1016\/j.joca.2022.09.011_bib19","doi-asserted-by":"crossref","first-page":"153","DOI":"10.1016\/S0195-5616(02)00083-9","article-title":"Laboratory evaluation and interpretation of synovial fluid","volume":"33","author":"MacWilliams","year":"2003","journal-title":"Vet Clin North Am Small Anim Pract"},{"issue":"3","key":"10.1016\/j.joca.2022.09.011_bib20","first-page":"183","article-title":"Isolation of human umbilical vein endothelial cells (HUVEC)","author":"Crampton","year":"2007","journal-title":"J\u00a0Vis Exp"},{"key":"10.1016\/j.joca.2022.09.011_bib21","doi-asserted-by":"crossref","first-page":"1851","DOI":"10.1016\/j.joca.2014.08.004","article-title":"Comparison of periprosthetic tissues in knee and hip joints: differential expression of CCL3 and DC-STAMP in total knee and hip arthroplasty and similar cytokine profiles in primary knee and hip osteoarthritis","volume":"22","author":"Tomankova","year":"2014","journal-title":"Osteoarthr Cartil"},{"issue":"5","key":"10.1016\/j.joca.2022.09.011_bib22","doi-asserted-by":"crossref","first-page":"317","DOI":"10.1078\/0344-0338-5710261","article-title":"Grading of chronic synovitis--a histopathological grading system for molecular and diagnostic pathology","volume":"198","author":"Krenn","year":"2002","journal-title":"Pathol Res Pract"},{"issue":"11","key":"10.1016\/j.joca.2022.09.011_bib23","doi-asserted-by":"crossref","first-page":"3492","DOI":"10.1002\/art.21373","article-title":"Macroscopic and microscopic features of synovial membrane inflammation in the osteoarthritic knee: correlating magnetic resonance imaging findings with disease severity","volume":"52","author":"Loeuille","year":"2005","journal-title":"Arthritis Rheum"},{"key":"10.1016\/j.joca.2022.09.011_bib24","doi-asserted-by":"crossref","first-page":"2004","DOI":"10.1002\/art.27475","volume":"62","author":"Liu-Bryan","year":"2010","journal-title":"Arthritis Rheum"},{"key":"10.1016\/j.joca.2022.09.011_bib25","doi-asserted-by":"crossref","first-page":"2171","DOI":"10.3389\/fimmu.2018.02171","article-title":"Update on neutrophil function in severe inflammation","volume":"9","author":"Mortaz","year":"2018","journal-title":"Front Immunol"},{"key":"10.1016\/j.joca.2022.09.011_bib26","doi-asserted-by":"crossref","first-page":"316","DOI":"10.4161\/viru.2.4.16142","article-title":"Role of TLR-\/NLR-signaling and the associated cytokines involved in recruitment of neutrophils in murine models of Staphylococcus aureus infection","volume":"2","author":"Sethi","year":"2011","journal-title":"Virulence"},{"key":"10.1016\/j.joca.2022.09.011_bib27","doi-asserted-by":"crossref","DOI":"10.1038\/s41598-020-75108-3","article-title":"Staphylococcus aureus secretes immunomodulatory RNA and DNA via membrane vesicles","volume":"10","author":"Rodriguez","year":"2020","journal-title":"Sci Rep"},{"issue":"2","key":"10.1016\/j.joca.2022.09.011_bib28","doi-asserted-by":"crossref","first-page":"42","DOI":"10.4103\/jgid.jgid_130_16","article-title":"Diagnostic accuracy of CD64 for sepsis in emergency department","volume":"10","author":"Dal Ponte","year":"2018","journal-title":"J\u00a0Glob Infect Dis"},{"key":"10.1016\/j.joca.2022.09.011_bib29","doi-asserted-by":"crossref","first-page":"1024","DOI":"10.1016\/j.freeradbiomed.2015.10.398","article-title":"Neutrophil NET formation is regulated from the inside by myeloperoxidase-processed reactive oxygen species","volume":"89","author":"Bj\u00f6rnsdottir","year":"2015","journal-title":"Free Radic Biol Med"},{"key":"10.1016\/j.joca.2022.09.011_bib30","doi-asserted-by":"crossref","DOI":"10.3389\/fimmu.2020.578129","article-title":"Neutrophil extracellular traps tied to rheumatoid arthritis: points to ponder","volume":"11","author":"Song","year":"2021","journal-title":"Front Immunol"},{"key":"10.1016\/j.joca.2022.09.011_bib31","doi-asserted-by":"crossref","first-page":"163","DOI":"10.1093\/gerona\/glw062","article-title":"Reduced oxidative burst by primed neutrophils in the elderly individuals is associated with increased levels of the CD16bright\/CD62Ldim immunosuppressive subset","volume":"72","author":"Sauce","year":"2017","journal-title":"J\u00a0Gerontol A Biol Sci Med Sci"},{"key":"10.1016\/j.joca.2022.09.011_bib32","doi-asserted-by":"crossref","DOI":"10.3389\/fphar.2021.644270","article-title":"Targeting neutrophils in sepsis: from mechanism to translation","volume":"12","author":"Shen","year":"2021","journal-title":"Front Pharmacol"},{"key":"10.1016\/j.joca.2022.09.011_bib33","doi-asserted-by":"crossref","first-page":"853","DOI":"10.1007\/s00011-016-0970-x","article-title":"Role of cellular events in the pathophysiology of sepsis","volume":"65","author":"Bhan","year":"2016","journal-title":"Inflamm Res"},{"key":"10.1016\/j.joca.2022.09.011_bib34","doi-asserted-by":"crossref","first-page":"1368","DOI":"10.1038\/nm1665","article-title":"CD16 promotes Escherichia coli sepsis through an FcR gamma inhibitory pathway that prevents phagocytosis and facilitates inflammation","volume":"13","author":"Pinheiro da Silva","year":"2007","journal-title":"Nat Med"},{"key":"10.1016\/j.joca.2022.09.011_bib35","doi-asserted-by":"crossref","DOI":"10.1371\/journal.ppat.1004644","article-title":"Bacterial immune evasion through manipulation of host inhibitory immune signaling","volume":"11","author":"Van Avondt","year":"2015","journal-title":"PLoS Pathog"},{"key":"10.1016\/j.joca.2022.09.011_bib36","doi-asserted-by":"crossref","DOI":"10.1038\/s41598-017-13417-w","article-title":"Vascular endothelial growth factor in cartilage development and osteoarthritis","volume":"7","author":"Nagao","year":"2017","journal-title":"Sci Rep"},{"issue":"3","key":"10.1016\/j.joca.2022.09.011_bib37","doi-asserted-by":"crossref","first-page":"158","DOI":"10.1038\/s41584-021-00738-4","article-title":"The role of neutrophils in rheumatic disease-associated vascular inflammation","volume":"18","author":"Wang","year":"2022","journal-title":"Nat Rev Rheumatol"},{"key":"10.1016\/j.joca.2022.09.011_bib38","doi-asserted-by":"crossref","first-page":"768","DOI":"10.1038\/labinvest.2013.71","article-title":"Expression of CXCL1 in human endothelial cells induces angiogenesis through the CXCR2 receptor and the ERK1\/2 and EGF pathways","volume":"93","author":"Miyake","year":"2013","journal-title":"Lab Invest"},{"issue":"5","key":"10.1016\/j.joca.2022.09.011_bib39","doi-asserted-by":"crossref","DOI":"10.1016\/j.acthis.2022.151909","article-title":"Preliminary study on immune cells in the synovium of end-stage osteoarthritis and rheumatoid arthritis patients: neutrophils and IgG4-secreting plasma cells as differential diagnosis candidates","volume":"124","author":"Dolzani","year":"2022","journal-title":"Acta Histochem"},{"key":"10.1016\/j.joca.2022.09.011_bib40","doi-asserted-by":"crossref","first-page":"23","DOI":"10.1016\/j.actbio.2021.12.006","article-title":"The immune microenvironment in cartilage injury and repair","volume":"140","author":"Li","year":"2022","journal-title":"Acta Biomater"},{"key":"10.1016\/j.joca.2022.09.011_bib41","doi-asserted-by":"crossref","first-page":"20","DOI":"10.1177\/1759720X13514669","article-title":"Targeting the synovial angiogenesis as a novel treatment approach to osteoarthritis","volume":"6","author":"Henrotin","year":"2014","journal-title":"Ther Adv Musculoskelet Dis"}],"container-title":["Osteoarthritis and Cartilage"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1063458422008639?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1063458422008639?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,10,21]],"date-time":"2025-10-21T03:59:25Z","timestamp":1761019165000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S1063458422008639"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,1]]},"references-count":41,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2023,1]]}},"alternative-id":["S1063458422008639"],"URL":"https:\/\/doi.org\/10.1016\/j.joca.2022.09.011","relation":{},"ISSN":["1063-4584"],"issn-type":[{"value":"1063-4584","type":"print"}],"subject":[],"published":{"date-parts":[[2023,1]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Phenotypic and functional characterisation of synovial fluid-derived neutrophils in knee osteoarthritis and knee infection","name":"articletitle","label":"Article Title"},{"value":"Osteoarthritis and Cartilage","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.joca.2022.09.011","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2022 Osteoarthritis Research Society International. Published by Elsevier Ltd.","name":"copyright","label":"Copyright"}]}}