{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T12:40:18Z","timestamp":1792068018301,"version":"4.4.0"},"reference-count":41,"publisher":"Elsevier BV","issue":"1","license":[{"start":{"date-parts":[[2025,1,1]],"date-time":"2025-01-01T00:00:00Z","timestamp":1735689600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2025,1,1]],"date-time":"2025-01-01T00:00:00Z","timestamp":1735689600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2024,9,15]],"date-time":"2024-09-15T00:00:00Z","timestamp":1726358400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000069","name":"National Institute of Arthritis and Musculoskeletal and Skin Diseases","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000069","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000980","name":"Arthritis Foundation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000980","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["clinicalkey.fr","clinicalkey.jp","clinicalkey.es","clinicalkey.com.au","oarsijournal.com","clinicalkey.com","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Osteoarthritis and Cartilage"],"published-print":{"date-parts":[[2025,1]]},"DOI":"10.1016\/j.joca.2024.09.002","type":"journal-article","created":{"date-parts":[[2024,9,16]],"date-time":"2024-09-16T16:26:43Z","timestamp":1726504003000},"page":"116-127","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":28,"title":["Degradomics defines proteolysis information flow from human knee osteoarthritis cartilage to matched synovial fluid and the contributions of secreted proteases ADAMTS5, MMP13 and CMA1 to articular cartilage breakdown"],"prefix":"10.1016","volume":"33","author":[{"given":"Sumit","family":"Bhutada","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6517-2546","authenticated-orcid":false,"given":"Anna","family":"Hoyle","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nicolas S.","family":"Piuzzi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Suneel S.","family":"Apte","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"issue":"6","key":"10.1016\/j.joca.2024.09.002_bib1","doi-asserted-by":"crossref","first-page":"492","DOI":"10.2106\/JBJS.22.00693","article-title":"A call to action for musculoskeletal research funding: the growing economic and disease burden of musculoskeletal conditions in the United States is not reflected in musculoskeletal research funding","volume":"105","author":"McConaghy","year":"2023","journal-title":"J Bone Joint Surg Am"},{"issue":"9","key":"10.1016\/j.joca.2024.09.002_bib2","doi-asserted-by":"crossref","first-page":"e508","DOI":"10.1016\/S2665-9913(23)00163-7","article-title":"Global, regional, and national burden of osteoarthritis, 1990-2020 and projections to 2050: a systematic analysis for the Global Burden of Disease Study 2021","volume":"5","author":"GBD 2021 Osteoarthritis Collaborators","year":"2023","journal-title":"Lancet Rheumatol"},{"issue":"2","key":"10.1016\/j.joca.2024.09.002_bib3","doi-asserted-by":"crossref","first-page":"C377","DOI":"10.1152\/ajpcell.00464.2022","article-title":"Cartilage extracellular matrix-derived matrikines in osteoarthritis","volume":"324","author":"Rapp","year":"2023","journal-title":"Am J Physiol Cell Physiol"},{"issue":"8","key":"10.1016\/j.joca.2024.09.002_bib4","doi-asserted-by":"crossref","first-page":"1091","DOI":"10.1016\/j.joca.2022.02.622","article-title":"Forward and reverse degradomics defines the proteolytic landscape of human knee osteoarthritic cartilage and the role of the serine protease HtrA1","volume":"30","author":"Bhutada","year":"2022","journal-title":"Osteoarthritis Cartilage"},{"issue":"1","key":"10.1016\/j.joca.2024.09.002_bib5","doi-asserted-by":"crossref","first-page":"1910","DOI":"10.1038\/s41467-023-37598-3","article-title":"Tryptase \u03b2 regulation of joint lubrication and inflammation via proteoglycan-4 in osteoarthritis","volume":"14","author":"Das","year":"2023","journal-title":"Nat Commun"},{"issue":"15","key":"10.1016\/j.joca.2024.09.002_bib6","doi-asserted-by":"crossref","DOI":"10.1002\/pmic.202300040","article-title":"Identification and quantification of degradome components in human synovial fluid reveals an increased proteolytic activity in knee osteoarthritis patients vs controls","volume":"23","author":"Ryd\u00e9n","year":"2023","journal-title":"Proteomics"},{"issue":"1","key":"10.1016\/j.joca.2024.09.002_bib7","doi-asserted-by":"crossref","DOI":"10.1016\/j.berh.2023.101848","article-title":"Synovial fluid analysis: relevance for daily clinical practice","volume":"37","author":"Oliviero","year":"2023","journal-title":"Best Pract Res Clin Rheumatol"},{"issue":"1","key":"10.1016\/j.joca.2024.09.002_bib8","doi-asserted-by":"crossref","first-page":"124","DOI":"10.1016\/j.joca.2020.09.005","article-title":"Identification of tissue-dependent proteins in knee OA synovial fluid","volume":"29","author":"Timur","year":"2021","journal-title":"Osteoarthritis Cartilage"},{"issue":"1","key":"10.1016\/j.joca.2024.09.002_bib9","doi-asserted-by":"crossref","first-page":"121","DOI":"10.1111\/febs.16127","article-title":"Matrix metalloproteinase-13 is fully activated by neutrophil elastase and inactivates its serpin inhibitor, alpha-1 antitrypsin: implications for osteoarthritis","volume":"289","author":"Wilkinson","year":"2022","journal-title":"FEBS J"},{"issue":"8","key":"10.1016\/j.joca.2024.09.002_bib10","doi-asserted-by":"crossref","first-page":"2243","DOI":"10.4049\/jimmunol.2000448","article-title":"The metalloproteinase ADAMTS5 is expressed by interstitial inflammatory cells in IgA nephropathy and is proteolytically active on the kidney matrix","volume":"205","author":"Taylor","year":"2020","journal-title":"J Immunol"},{"issue":"Database issue","key":"10.1016\/j.joca.2024.09.002_bib11","doi-asserted-by":"crossref","first-page":"D290","DOI":"10.1093\/nar\/gku1012","article-title":"Proteome TopFIND 3.0 with TopFINDer and PathFINDer: database and analysis tools for the association of protein termini to pre- and post-translational events","volume":"43","author":"Fortelny","year":"2015","journal-title":"Nucleic Acids Res"},{"issue":"9","key":"10.1016\/j.joca.2024.09.002_bib12","doi-asserted-by":"crossref","first-page":"703","DOI":"10.1038\/nmeth.1669","article-title":"TopFIND, a knowledgebase linking protein termini with function","volume":"8","author":"Lange","year":"2011","journal-title":"Nat Methods"},{"issue":"D1","key":"10.1016\/j.joca.2024.09.002_bib13","doi-asserted-by":"crossref","first-page":"D624","DOI":"10.1093\/nar\/gkx1134","article-title":"The MEROPS database of proteolytic enzymes, their substrates and inhibitors in 2017 and a comparison with peptidases in the PANTHER database","volume":"46","author":"Rawlings","year":"2018","journal-title":"Nucleic Acids Res"},{"issue":"1","key":"10.1016\/j.joca.2024.09.002_bib14","doi-asserted-by":"crossref","first-page":"83","DOI":"10.1002\/pro.3948","article-title":"How to use the MEROPS database and website to help understand peptidase specificity","volume":"30","author":"Rawlings","year":"2021","journal-title":"Protein Sci"},{"issue":"18","key":"10.1016\/j.joca.2024.09.002_bib15","doi-asserted-by":"crossref","first-page":"2938","DOI":"10.1093\/bioinformatics\/btx364","article-title":"UpSetR: an R package for the visualization of intersecting sets and their properties","volume":"33","author":"Conway","year":"2017","journal-title":"Bioinformatics"},{"key":"10.1016\/j.joca.2024.09.002_bib16","series-title":"ggplot2: Elegant Graphics for Data Analysis","author":"Wickham","year":"2016"},{"issue":"2","key":"10.1016\/j.joca.2024.09.002_bib17","doi-asserted-by":"crossref","first-page":"157","DOI":"10.1016\/S0006-291X(67)80055-X","article-title":"On the size of the active site in proteases. I. Papain","volume":"27","author":"Schechter","year":"1967","journal-title":"Biochem Biophys Res Commun"},{"key":"10.1016\/j.joca.2024.09.002_bib18","doi-asserted-by":"crossref","first-page":"37","DOI":"10.1016\/j.matbio.2015.09.003","article-title":"Active site specificity profiling of the matrix metalloproteinase family: proteomic identification of 4300 cleavage sites by nine MMPs explored with structural and synthetic peptide cleavage analyses","volume":"49","author":"Eckhard","year":"2016","journal-title":"Matrix Biol"},{"issue":"4","key":"10.1016\/j.joca.2024.09.002_bib19","doi-asserted-by":"crossref","first-page":"363","DOI":"10.1042\/cs0930363","article-title":"Mast cell activation in arthritis: detection of alpha- and beta-tryptase, histamine and eosinophil cationic protein in synovial fluid","volume":"93","author":"Buckley","year":"1997","journal-title":"Clin Sci"},{"issue":"4","key":"10.1016\/j.joca.2024.09.002_bib20","first-page":"135","article-title":"Cellular activation products in osteoarthritis synovial fluid","volume":"15","author":"Renoux","year":"1995","journal-title":"Int J Clin Pharmacol Res"},{"issue":"1","key":"10.1016\/j.joca.2024.09.002_bib21","doi-asserted-by":"crossref","first-page":"75","DOI":"10.1186\/s40634-021-00391-6","article-title":"A novel mice model of acute flares in osteoarthritis elicited by intra-articular injection of cultured mast cells","volume":"8","author":"Dan","year":"2021","journal-title":"J Exp Orthop"},{"issue":"4","key":"10.1016\/j.joca.2024.09.002_bib22","doi-asserted-by":"crossref","first-page":"664","DOI":"10.1016\/j.joca.2015.11.011","article-title":"Characterization of synovial mast cells in knee osteoarthritis: association with clinical parameters","volume":"24","author":"de Lange-Brokaar","year":"2016","journal-title":"Osteoarthritis Cartilage"},{"issue":"6","key":"10.1016\/j.joca.2024.09.002_bib23","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0252590","article-title":"Bone marrow derived mast cells injected into the osteoarthritic knee joints of mice induced by sodium monoiodoacetate enhanced spontaneous pain through activation of PAR2 and action of extracellular ATP","volume":"16","author":"Habuchi","year":"2021","journal-title":"PLoS One"},{"issue":"1","key":"10.1016\/j.joca.2024.09.002_bib24","doi-asserted-by":"crossref","first-page":"541","DOI":"10.3390\/ijms23010541","article-title":"Mast cells differentiated in synovial fluid and resident in osteophytes exalt the inflammatory pathology of osteoarthritis","volume":"23","author":"Kulkarni","year":"2022","journal-title":"Int J Mol Sci"},{"key":"10.1016\/j.joca.2024.09.002_bib25","doi-asserted-by":"crossref","DOI":"10.7554\/eLife.39905","article-title":"IgE-mediated mast cell activation promotes inflammation and cartilage destruction in osteoarthritis","volume":"8","author":"Wang","year":"2019","journal-title":"Elife"},{"issue":"4","key":"10.1016\/j.joca.2024.09.002_bib26","doi-asserted-by":"crossref","first-page":"2523","DOI":"10.4049\/jimmunol.1003383","article-title":"Mast cells as sensors of cell injury through IL-33 recognition","volume":"186","author":"Enoksson","year":"2011","journal-title":"J Immunol"},{"issue":"10","key":"10.1016\/j.joca.2024.09.002_bib27","doi-asserted-by":"crossref","DOI":"10.1002\/cti2.1187","article-title":"Blockade of IL-33 signalling attenuates osteoarthritis","volume":"9","author":"He","year":"2020","journal-title":"Clin Transl Immunology"},{"issue":"9","key":"10.1016\/j.joca.2024.09.002_bib28","doi-asserted-by":"crossref","first-page":"5690","DOI":"10.3390\/ijerph19095690","article-title":"Counteractive effects of IL-33 and IL-37 on inflammation in osteoarthritis","volume":"19","author":"Rai","year":"2022","journal-title":"Int J Environ Res Public Health"},{"key":"10.1016\/j.joca.2024.09.002_bib29","doi-asserted-by":"crossref","DOI":"10.3389\/fimmu.2022.918305","article-title":"Mast cell and basophil granule proteases \u2013 in vivo targets and function","volume":"13","author":"Hellman","year":"2022","journal-title":"Front Immunol"},{"issue":"1","key":"10.1016\/j.joca.2024.09.002_bib30","doi-asserted-by":"crossref","first-page":"95","DOI":"10.1093\/intimm\/dxn128","article-title":"The extended substrate specificity of the human mast cell chymase reveals a serine protease with well-defined substrate recognition profile","volume":"21","author":"Andersson","year":"2009","journal-title":"Int Immunol"},{"issue":"12","key":"10.1016\/j.joca.2024.09.002_bib31","doi-asserted-by":"crossref","first-page":"3723","DOI":"10.1002\/art.25002","article-title":"Matrix metalloproteinase 13-deficient mice are resistant to osteoarthritic cartilage erosion but not chondrocyte hypertrophy or osteophyte development","volume":"60","author":"Little","year":"2009","journal-title":"Arthritis Rheum"},{"issue":"1","key":"10.1016\/j.joca.2024.09.002_bib32","doi-asserted-by":"crossref","first-page":"133","DOI":"10.1016\/j.bbapap.2011.06.020","article-title":"Proteases involved in cartilage matrix degradation in osteoarthritis","volume":"1824","author":"Troeberg","year":"2012","journal-title":"Biochim Biophys Acta"},{"issue":"15","key":"10.1016\/j.joca.2024.09.002_bib33","doi-asserted-by":"crossref","first-page":"3020","DOI":"10.1038\/sj.emboj.7600318","article-title":"Collagenase unwinds triple-helical collagen prior to peptide bond hydrolysis","volume":"23","author":"Chung","year":"2004","journal-title":"EMBO J"},{"issue":"7033","key":"10.1016\/j.joca.2024.09.002_bib34","doi-asserted-by":"crossref","first-page":"644","DOI":"10.1038\/nature03369","article-title":"Deletion of active ADAMTS5 prevents cartilage degradation in a murine model of osteoarthritis","volume":"434","author":"Glasson","year":"2005","journal-title":"Nature"},{"issue":"25","key":"10.1016\/j.joca.2024.09.002_bib35","doi-asserted-by":"crossref","first-page":"22201","DOI":"10.1074\/jbc.M200431200","article-title":"Inhibition of ADAM-TS4 and ADAM-TS5 prevents aggrecan degradation in osteoarthritic cartilage","volume":"277","author":"Malfait","year":"2002","journal-title":"J Biol Chem"},{"issue":"7033","key":"10.1016\/j.joca.2024.09.002_bib36","doi-asserted-by":"crossref","first-page":"648","DOI":"10.1038\/nature03417","article-title":"ADAMTS5 is the major aggrecanase in mouse cartilage in vivo and in vitro","volume":"434","author":"Stanton","year":"2005","journal-title":"Nature"},{"issue":"33","key":"10.1016\/j.joca.2024.09.002_bib37","doi-asserted-by":"crossref","first-page":"23443","DOI":"10.1074\/jbc.274.33.23443","article-title":"Cloning and characterization of ADAMTS11, an aggrecanase from the ADAMTS family","volume":"274","author":"Abbaszade","year":"1999","journal-title":"J Biol Chem"},{"key":"10.1016\/j.joca.2024.09.002_bib38","doi-asserted-by":"crossref","DOI":"10.1016\/j.jprot.2021.104358","article-title":"Identification of novel ADAMTS1, ADAMTS4 and ADAMTS5 cleavage sites in versican using a label-free quantitative proteomics approach","volume":"249","author":"Martin","year":"2021","journal-title":"J Proteomics"},{"issue":"24","key":"10.1016\/j.joca.2024.09.002_bib39","doi-asserted-by":"crossref","first-page":"18566","DOI":"10.1074\/jbc.M909383199","article-title":"Sites of aggrecan cleavage by recombinant human aggrecanase-1 (ADAMTS- 4)","volume":"275","author":"Tortorella","year":"2000","journal-title":"J Biol Chem"},{"issue":"7","key":"10.1016\/j.joca.2024.09.002_bib40","doi-asserted-by":"crossref","first-page":"1423","DOI":"10.1016\/j.ajpath.2019.03.013","article-title":"High-temperature requirement A1 protease as a rate-limiting factor in the development of osteoarthritis","volume":"189","author":"Chen","year":"2019","journal-title":"Am J Pathol"},{"issue":"3","key":"10.1016\/j.joca.2024.09.002_bib41","doi-asserted-by":"crossref","DOI":"10.1016\/j.mcpro.2022.100200","article-title":"Proteomics profiling of human synovial fluid suggests increased protein interplay in early-osteoarthritis (OA) that is lost in late-stage OA","volume":"21","author":"Ali","year":"2022","journal-title":"Mol Cell Proteomics"}],"container-title":["Osteoarthritis and Cartilage"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1063458424013979?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1063458424013979?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2024,12,18]],"date-time":"2024-12-18T17:11:50Z","timestamp":1734541910000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S1063458424013979"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,1]]},"references-count":41,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2025,1]]}},"alternative-id":["S1063458424013979"],"URL":"https:\/\/doi.org\/10.1016\/j.joca.2024.09.002","relation":{},"ISSN":["1063-4584"],"issn-type":[{"value":"1063-4584","type":"print"}],"subject":[],"published":{"date-parts":[[2025,1]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Degradomics defines proteolysis information flow from human knee osteoarthritis cartilage to matched synovial fluid and the contributions of secreted proteases ADAMTS5, MMP13 and CMA1 to articular cartilage breakdown","name":"articletitle","label":"Article Title"},{"value":"Osteoarthritis and Cartilage","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.joca.2024.09.002","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2024 The Author(s). Published by Elsevier Ltd on behalf of Osteoarthritis Research Society International.","name":"copyright","label":"Copyright"}]}}