{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T04:13:04Z","timestamp":1788235984979,"version":"build-2803163510"},"reference-count":42,"publisher":"Elsevier BV","issue":"9","license":[{"start":{"date-parts":[[2025,9,1]],"date-time":"2025-09-01T00:00:00Z","timestamp":1756684800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2025,9,1]],"date-time":"2025-09-01T00:00:00Z","timestamp":1756684800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T00:00:00Z","timestamp":1788220800000},"content-version":"vor","delay-in-days":365,"URL":"http:\/\/www.elsevier.com\/open-access\/userlicense\/1.0\/"}],"funder":[{"DOI":"10.13039\/501100017607","name":"Shenzhen Municipal Fundamental Research Program","doi-asserted-by":"publisher","award":["JCYJ20220818100617036"],"award-info":[{"award-number":["JCYJ20220818100617036"]}],"id":[{"id":"10.13039\/501100017607","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["2019YFA0906004"],"award-info":[{"award-number":["2019YFA0906004"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81991513"],"award-info":[{"award-number":["81991513"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82250710175"],"award-info":[{"award-number":["82250710175"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82230081"],"award-info":[{"award-number":["82230081"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82261160395"],"award-info":[{"award-number":["82261160395"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82430078"],"award-info":[{"award-number":["82430078"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["clinicalkey.com","clinicalkey.com.au","clinicalkey.es","clinicalkey.fr","clinicalkey.jp","oarsijournal.com","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Osteoarthritis and Cartilage"],"published-print":{"date-parts":[[2025,9]]},"DOI":"10.1016\/j.joca.2025.06.003","type":"journal-article","created":{"date-parts":[[2025,6,13]],"date-time":"2025-06-13T12:03:12Z","timestamp":1749816192000},"page":"1095-1106","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":0,"title":["Effects of tamoxifen on fetal cartilage development, adult cartilage metabolism and endochondral ossification in mice"],"prefix":"10.1016","volume":"33","author":[{"given":"Rongdong","family":"Liao","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jianglong","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chao","family":"Xie","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qinnan","family":"Yan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Donghao","family":"Gan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guozhi","family":"Xiao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lijun","family":"Lin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"issue":"1","key":"10.1016\/j.joca.2025.06.003_bib1","doi-asserted-by":"crossref","first-page":"135","DOI":"10.1152\/physrev.00033.2015","article-title":"Estrogens and androgens in skeletal physiology and pathophysiology","volume":"97","author":"Almeida","year":"2017","journal-title":"Physiol Rev"},{"issue":"5","key":"10.1016\/j.joca.2025.06.003_bib2","doi-asserted-by":"crossref","first-page":"333","DOI":"10.1016\/j.tig.2017.12.008","article-title":"Detecting and avoiding problems when using the Cre-lox system","volume":"34","author":"Song","year":"2018","journal-title":"Trends Genet"},{"issue":"4","key":"10.1016\/j.joca.2025.06.003_bib3","doi-asserted-by":"crossref","first-page":"147","DOI":"10.5625\/lar.2018.34.4.147","article-title":"Mouse Cre-LoxP system: general principles to determine tissue-specific roles of target genes","volume":"34","author":"Kim","year":"2018","journal-title":"Lab Anim Res"},{"issue":"5229","key":"10.1016\/j.joca.2025.06.003_bib4","doi-asserted-by":"crossref","first-page":"1427","DOI":"10.1126\/science.7660125","article-title":"Inducible gene targeting in mice","volume":"269","author":"K\u00fchn","year":"1995","journal-title":"Science"},{"issue":"4","key":"10.1016\/j.joca.2025.06.003_bib5","doi-asserted-by":"crossref","first-page":"339","DOI":"10.1101\/pdb.top069807","article-title":"Strategies to achieve conditional gene mutation in mice","volume":"2014","author":"Gierut","year":"2014","journal-title":"Cold Spring Harb Protoc"},{"key":"10.1016\/j.joca.2025.06.003_bib6","doi-asserted-by":"crossref","DOI":"10.1016\/j.biopha.2023.115045","article-title":"A review of tamoxifen administration regimen optimization for Cre\/loxp system in mouse bone study","volume":"165","author":"Chen","year":"2023","journal-title":"Biomed Pharmacother"},{"key":"10.1016\/j.joca.2025.06.003_bib7","doi-asserted-by":"crossref","first-page":"57","DOI":"10.1016\/j.mce.2017.05.012","article-title":"A shortened tamoxifen induction scheme to induce CreER recombinase without side effects on the male mouse skeleton","volume":"452","author":"Jardi","year":"2017","journal-title":"Mol Cell Endocrinol"},{"issue":"5","key":"10.1016\/j.joca.2025.06.003_bib8","first-page":"181","article-title":"Tamoxifen prevents bone loss in castrated male mice","volume":"32","author":"Broulik","year":"2000","journal-title":"Horm\u00a0Metab\u00a0Res"},{"issue":"4","key":"10.1016\/j.joca.2025.06.003_bib9","first-page":"217","article-title":"Tamoxifen prevents bone loss in ovariectomized mice","volume":"25","author":"Broulik","year":"1991","journal-title":"Endocr\u00a0Regul"},{"key":"10.1016\/j.joca.2025.06.003_bib10","doi-asserted-by":"crossref","first-page":"614","DOI":"10.1016\/j.bone.2015.07.034","article-title":"Optimizing tamoxifen-inducible Cre\/loxp system to reduce tamoxifen effect on bone turnover in long bones of young mice","volume":"81","author":"Zhong","year":"2015","journal-title":"Bone"},{"issue":"14","key":"10.1016\/j.joca.2025.06.003_bib11","doi-asserted-by":"crossref","first-page":"7829","DOI":"10.1073\/pnas.130200197","article-title":"Estrogens suppress RANK ligand-induced osteoclast differentiation via a stromal cell independent mechanism involving c-Jun repression","volume":"97","author":"Shevde","year":"2000","journal-title":"Proc Natl Acad Sci U S A"},{"issue":"7","key":"10.1016\/j.joca.2025.06.003_bib12","doi-asserted-by":"crossref","first-page":"637","DOI":"10.1001\/jama.282.7.637","article-title":"Reduction of vertebral fracture risk in postmenopausal women with osteoporosis treated with raloxifene: results from a 3-year randomized clinical trial. Multiple Outcomes of Raloxifene Evaluation (MORE) Investigators","volume":"282","author":"Ettinger","year":"1999","journal-title":"JAMA"},{"issue":"3","key":"10.1016\/j.joca.2025.06.003_bib13","doi-asserted-by":"crossref","first-page":"1060","DOI":"10.1210\/en.2004-1114","article-title":"Tamoxifen stimulates cancellous bone formation in long bones of female mice","volume":"146","author":"Perry","year":"2005","journal-title":"Endocrinology"},{"issue":"2","key":"10.1016\/j.joca.2025.06.003_bib14","doi-asserted-by":"crossref","first-page":"127","DOI":"10.1016\/0890-6238(88)90008-1","article-title":"Permanent chondrification in the pelvis and occurrence of hernias in mice treated neonatally with tamoxifen","volume":"2","author":"Iguchi","year":"1988","journal-title":"Reprod Toxicol"},{"issue":"8","key":"10.1016\/j.joca.2025.06.003_bib15","doi-asserted-by":"crossref","first-page":"1267","DOI":"10.1359\/jbmr.080319","article-title":"Tamoxifen impairs both longitudinal and cortical bone growth in young male rats","volume":"23","author":"Karimian","year":"2008","journal-title":"J Bone Miner Res"},{"issue":"5","key":"10.1016\/j.joca.2025.06.003_bib16","doi-asserted-by":"crossref","first-page":"1415","DOI":"10.1016\/j.bone.2006.12.066","article-title":"Tamoxifen induces permanent growth arrest through selective induction of apoptosis in growth plate chondrocytes in cultured rat metatarsal bones","volume":"40","author":"Chagin","year":"2007","journal-title":"Bone"},{"key":"10.1016\/j.joca.2025.06.003_bib17","doi-asserted-by":"crossref","first-page":"12","DOI":"10.1016\/j.jot.2023.05.005","article-title":"Comparison of Kindlin-2 deficiency-stimulated osteoarthritis-like lesions induced by Prg4(CreERT2) versus Aggrecan(CreERT2) transgene in mice","volume":"41","author":"Yao","year":"2023","journal-title":"J Orthop Translat"},{"key":"10.1016\/j.joca.2025.06.003_bib18","doi-asserted-by":"crossref","first-page":"105","DOI":"10.1016\/j.jare.2023.09.040","article-title":"Piezo1 activation accelerates osteoarthritis progression and the targeted therapy effect of artemisinin","volume":"62","author":"Gan","year":"2024","journal-title":"J Adv Res"},{"issue":"8","key":"10.1016\/j.joca.2025.06.003_bib19","doi-asserted-by":"crossref","DOI":"10.1172\/JCI148073","article-title":"Age-associated callus senescent cells produce TGF-\u03b21 that inhibits fracture healing in aged mice","volume":"132","author":"Liu","year":"2022","journal-title":"J Clin Invest"},{"issue":"46","key":"10.1016\/j.joca.2025.06.003_bib20","article-title":"Insufficient mechanical loading downregulates Piezo1 in chondrocytes and impairs fracture healing through ApoE-induced senescence","volume":"11","author":"Jia","year":"2024","journal-title":"Adv Sci (Weinh)"},{"key":"10.1016\/j.joca.2025.06.003_bib21","doi-asserted-by":"crossref","first-page":"12","DOI":"10.1016\/j.bone.2014.08.016","article-title":"Disruption of glucocorticoid signaling in chondrocytes delays metaphyseal fracture healing but does not affect normal cartilage and bone development","volume":"69","author":"Tu","year":"2014","journal-title":"Bone"},{"key":"10.1016\/j.joca.2025.06.003_bib22","doi-asserted-by":"crossref","DOI":"10.1016\/j.bone.2022.116599","article-title":"AcanCreER lacks specificity to chondrocytes and targets periosteal progenitors in the fractured callus","volume":"166","author":"Novak","year":"2023","journal-title":"Bone"},{"issue":"2","key":"10.1016\/j.joca.2025.06.003_bib23","doi-asserted-by":"crossref","first-page":"97","DOI":"10.1007\/s00418-023-02196-3","article-title":"Maternal tamoxifen exposure leads to abnormal primordial follicle assembly","volume":"160","author":"Zhao","year":"2023","journal-title":"Histochem Cell Biol"},{"issue":"5","key":"10.1016\/j.joca.2025.06.003_bib24","doi-asserted-by":"crossref","DOI":"10.1098\/rsob.180262","article-title":"Osteocyte TSC1 promotes sclerostin secretion to restrain osteogenesis in mice","volume":"9","author":"Liu","year":"2019","journal-title":"Open Biol"},{"key":"10.1016\/j.joca.2025.06.003_bib25","doi-asserted-by":"crossref","first-page":"2","DOI":"10.1038\/s41413-019-0073-8","article-title":"Focal adhesion protein Kindlin-2 regulates bone homeostasis in mice","volume":"8","author":"Cao","year":"2020","journal-title":"Bone Res"},{"issue":"2","key":"10.1016\/j.joca.2025.06.003_bib26","doi-asserted-by":"crossref","first-page":"305","DOI":"10.1006\/dbio.2002.0597","article-title":"Efficient recombination in diverse tissues by a tamoxifen-inducible form of Cre: a tool for temporally regulated gene activation\/inactivation in the mouse","volume":"244","author":"Hayashi","year":"2002","journal-title":"Dev Biol"},{"issue":"22","key":"10.1016\/j.joca.2025.06.003_bib27","doi-asserted-by":"crossref","DOI":"10.3390\/ijms232214077","article-title":"Limitations of tamoxifen application for in vivo genome editing using Cre\/ER(T2) system","volume":"23","author":"Ilchuk","year":"2022","journal-title":"Int J Mol Sci"},{"issue":"1","key":"10.1016\/j.joca.2025.06.003_bib28","doi-asserted-by":"crossref","first-page":"8214","DOI":"10.1038\/s41598-021-87611-2","article-title":"Activation of creER recombinase in the mouse calvaria induces local recombination without effects on distant skeletal segments","volume":"11","author":"Hou","year":"2021","journal-title":"Sci Rep"},{"issue":"6937","key":"10.1016\/j.joca.2025.06.003_bib29","doi-asserted-by":"crossref","first-page":"332","DOI":"10.1038\/nature01657","article-title":"Developmental regulation of the growth plate","volume":"423","author":"Kronenberg","year":"2003","journal-title":"Nature"},{"issue":"6","key":"10.1016\/j.joca.2025.06.003_bib30","doi-asserted-by":"crossref","first-page":"984","DOI":"10.1210\/endrev\/bnac008","article-title":"Fracture healing in the setting of endocrine diseases, aging, and cellular senescence","volume":"43","author":"Saul","year":"2022","journal-title":"Endocr Rev"},{"issue":"8","key":"10.1016\/j.joca.2025.06.003_bib31","doi-asserted-by":"crossref","DOI":"10.1073\/pnas.2019152118","article-title":"SOX9 keeps growth plates and articular cartilage healthy by inhibiting chondrocyte dedifferentiation\/osteoblastic redifferentiation","volume":"118","author":"Haseeb","year":"2021","journal-title":"Proc Natl Acad Sci U S A."},{"issue":"10","key":"10.1016\/j.joca.2025.06.003_bib32","doi-asserted-by":"crossref","DOI":"10.3390\/ijms23105776","article-title":"Whole aspect of Runx2 functions in skeletal development","volume":"23","author":"Komori","year":"2022","journal-title":"Int J Mol Sci"},{"issue":"1","key":"10.1016\/j.joca.2025.06.003_bib33","doi-asserted-by":"crossref","first-page":"95","DOI":"10.1002\/jbmr.2318","article-title":"SDF-1\/CXCR4 axis in Tie2-lineage cells including endothelial progenitor cells contributes to bone fracture healing","volume":"30","author":"Kawakami","year":"2015","journal-title":"J Bone Miner Res"},{"issue":"3","key":"10.1016\/j.joca.2025.06.003_bib34","first-page":"9792","article-title":"Development of monosodium acetate-induced osteoarthritis and inflammatory pain in ageing mice","volume":"37","author":"Ogbonna","year":"2015","journal-title":"Age\u00a0(Dordr)"},{"issue":"30","key":"10.1016\/j.joca.2025.06.003_bib35","article-title":"Zwitterion-lubricated hydrogel microspheres encapsulated with metformin ameliorate age-associated osteoarthritis","volume":"11","author":"Hou","year":"2024","journal-title":"Adv Sci\u00a0(Weinh)"},{"issue":"5","key":"10.1016\/j.joca.2025.06.003_bib36","doi-asserted-by":"crossref","first-page":"635","DOI":"10.1136\/annrheumdis-2019-216713","article-title":"Metformin limits osteoarthritis development and progression through activation of AMPK signalling","volume":"79","author":"Li","year":"2020","journal-title":"Ann Rheum Dis"},{"issue":"3","key":"10.1016\/j.joca.2025.06.003_bib37","doi-asserted-by":"crossref","DOI":"10.1002\/jbm4.10450","article-title":"Low-dose tamoxifen induces significant bone formation in mice","volume":"5","author":"Xie","year":"2021","journal-title":"JBMR Plus"},{"issue":"1","key":"10.1016\/j.joca.2025.06.003_bib38","doi-asserted-by":"crossref","first-page":"226","DOI":"10.1038\/s41366-019-0330-3","article-title":"Even a low dose of tamoxifen profoundly induces adipose tissue browning in female mice","volume":"44","author":"Zhao","year":"2020","journal-title":"Int J Obes"},{"issue":"6","key":"10.1016\/j.joca.2025.06.003_bib39","doi-asserted-by":"crossref","first-page":"695","DOI":"10.1016\/j.joca.2006.11.005","article-title":"Osteoarthritis severity is sex dependent in a surgical mouse model","volume":"15","author":"Ma","year":"2007","journal-title":"Osteoarthritis Cartilage"},{"issue":"7","key":"10.1016\/j.joca.2025.06.003_bib40","doi-asserted-by":"crossref","first-page":"6443","DOI":"10.3390\/ijms24076443","article-title":"Sex difference in cardioprotection against acute myocardial infarction in MAO-B knockout mice in vivo","volume":"24","author":"Heger","year":"2023","journal-title":"Int J Mol Sci"},{"issue":"1","key":"10.1016\/j.joca.2025.06.003_bib41","doi-asserted-by":"crossref","first-page":"164","DOI":"10.1007\/s12282-013-0469-z","article-title":"A case of delivery of healthy infant in breast cancer patient incidentally treated with goserelin acetate and tamoxifen during pregnancy","volume":"23","author":"Ishizuka","year":"2016","journal-title":"Breast Cancer"},{"issue":"1","key":"10.1016\/j.joca.2025.06.003_bib42","doi-asserted-by":"crossref","first-page":"17","DOI":"10.1007\/s10549-019-05154-7","article-title":"Tamoxifen and pregnancy: an absolute contraindication?","volume":"175","author":"Schuurman","year":"2019","journal-title":"Breast Cancer Res Treat"}],"container-title":["Osteoarthritis and Cartilage"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1063458425010544?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1063458425010544?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T03:14:01Z","timestamp":1788232441000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S1063458425010544"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,9]]},"references-count":42,"journal-issue":{"issue":"9","published-print":{"date-parts":[[2025,9]]}},"alternative-id":["S1063458425010544"],"URL":"https:\/\/doi.org\/10.1016\/j.joca.2025.06.003","relation":{},"ISSN":["1063-4584"],"issn-type":[{"value":"1063-4584","type":"print"}],"subject":[],"published":{"date-parts":[[2025,9]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Effects of tamoxifen on fetal cartilage development, adult cartilage metabolism and endochondral ossification in mice","name":"articletitle","label":"Article Title"},{"value":"Osteoarthritis and Cartilage","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.joca.2025.06.003","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2025 Osteoarthritis Research Society International. Published by Elsevier Ltd.","name":"copyright","label":"Copyright"}]}}