{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,17]],"date-time":"2026-10-17T19:41:16Z","timestamp":1792266076014,"version":"4.4.0"},"reference-count":52,"publisher":"Elsevier BV","issue":"2","license":[{"start":{"date-parts":[[2024,2,1]],"date-time":"2024-02-01T00:00:00Z","timestamp":1706745600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2024,2,1]],"date-time":"2024-02-01T00:00:00Z","timestamp":1706745600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"}],"funder":[{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004586","name":"Funda\u00e7\u00e3o Carlos Chagas Filho de Amparo \u00e0 Pesquisa do Estado do Rio de Janeiro","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004586","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["clinicalkey.com","clinicalkey.com.au","clinicalkey.es","clinicalkey.jp","jendodon.com","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Journal of Endodontics"],"published-print":{"date-parts":[[2024,2]]},"DOI":"10.1016\/j.joen.2023.11.005","type":"journal-article","created":{"date-parts":[[2023,11,15]],"date-time":"2023-11-15T18:50:55Z","timestamp":1700074255000},"page":"154-163","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":10,"title":["Bacteriologic Conditions of the Apical Root Canal System of Teeth with and without Posttreatment Apical Periodontitis: A Correlative Multianalytical Approach"],"prefix":"10.1016","volume":"50","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1702-0919","authenticated-orcid":false,"given":"Sandra R.","family":"Hern\u00e1ndez","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"suffix":"Jr.","given":"Jos\u00e9 F.","family":"Siqueira","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9555-3596","authenticated-orcid":false,"given":"Danielle D.","family":"Voigt","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Giuliana","family":"Soimu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sabrina C.","family":"Brasil","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4902-8664","authenticated-orcid":false,"given":"Jos\u00e9 C.","family":"Provenzano","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ibrahimu","family":"Mdala","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9922-8202","authenticated-orcid":false,"given":"Fl\u00e1vio R.F.","family":"Alves","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Isabela N.","family":"R\u00f4\u00e7as","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.joen.2023.11.005_bib1","doi-asserted-by":"crossref","first-page":"712","DOI":"10.1111\/iej.13467","article-title":"The global prevalence of apical periodontitis: a systematic review and meta-analysis","volume":"54","author":"Tiburcio-Machado","year":"2021","journal-title":"Int Endod J"},{"key":"10.1016\/j.joen.2023.11.005_bib2","doi-asserted-by":"crossref","first-page":"281","DOI":"10.1067\/moe.2002.126163","article-title":"Endodontic infections: concepts, paradigms, and perspectives","volume":"94","author":"Siqueira","year":"2002","journal-title":"Oral Surg Oral Med Oral Pathol Oral Radiol Endod"},{"key":"10.1016\/j.joen.2023.11.005_bib3","doi-asserted-by":"crossref","first-page":"583","DOI":"10.1111\/j.1365-2591.2011.01872.x","article-title":"A prospective study of the factors affecting outcomes of nonsurgical root canal treatment: part 1: periapical health","volume":"44","author":"Ng","year":"2011","journal-title":"Int Endod J"},{"key":"10.1016\/j.joen.2023.11.005_bib4","doi-asserted-by":"crossref","first-page":"1170","DOI":"10.1016\/j.joen.2012.05.023","article-title":"Prevalence of periapical radiolucency and root canal treatment: a systematic review of cross-sectional studies","volume":"38","author":"Pak","year":"2012","journal-title":"J Endod"},{"key":"10.1016\/j.joen.2023.11.005_bib5","series-title":"Endodontic Microbiology","first-page":"341","article-title":"Prognosis of healing in treated teeth with endodontic infections","author":"Friedman","year":"2017"},{"key":"10.1016\/j.joen.2023.11.005_bib6","doi-asserted-by":"crossref","first-page":"305","DOI":"10.1038\/sj.bdj.2014.200","article-title":"Causes and management of post-treatment apical periodontitis","volume":"216","author":"Siqueira","year":"2014","journal-title":"Br Dent J"},{"issue":"Suppl 3","key":"10.1016\/j.joen.2023.11.005_bib7","doi-asserted-by":"crossref","first-page":"512","DOI":"10.1111\/iej.13677","article-title":"Present status and future directions: Microbiology of endodontic infections","volume":"55","author":"Siqueira","year":"2022","journal-title":"Int Endod J"},{"key":"10.1016\/j.joen.2023.11.005_bib8","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1046\/j.1365-2591.2003.00603.x","article-title":"Microorganisms from canals of root-filled teeth with periapical lesions","volume":"36","author":"Pinheiro","year":"2003","journal-title":"Int Endod J"},{"key":"10.1016\/j.joen.2023.11.005_bib9","doi-asserted-by":"crossref","first-page":"2374","DOI":"10.3389\/fmicb.2018.02374","article-title":"Root Microbiota in Primary and Secondary Apical Periodontitis","volume":"9","author":"Bouillaguet","year":"2018","journal-title":"Front Microbiol"},{"key":"10.1016\/j.joen.2023.11.005_bib10","doi-asserted-by":"crossref","first-page":"1359","DOI":"10.1016\/j.joen.2011.06.020","article-title":"Molecular characterization of the microbial flora residing at the apical portion of infected root canals of human teeth","volume":"37","author":"Chugal","year":"2011","journal-title":"J Endod"},{"key":"10.1016\/j.joen.2023.11.005_bib11","doi-asserted-by":"crossref","first-page":"372","DOI":"10.1016\/j.joen.2017.11.019","article-title":"Microbial analysis of endodontic infections in root-filled teeth with apical periodontitis before and after irrigation using pyrosequencing","volume":"44","author":"Zandi","year":"2018","journal-title":"J Endod"},{"key":"10.1016\/j.joen.2023.11.005_bib12","doi-asserted-by":"crossref","first-page":"1195","DOI":"10.1016\/j.joen.2020.05.020","article-title":"The apical root canal system of teeth with posttreatment apical periodontitis: correlating microbiologic, tomographic, and histopathologic findings","volume":"46","author":"Siqueira","year":"2020","journal-title":"J Endod"},{"key":"10.1016\/j.joen.2023.11.005_bib13","doi-asserted-by":"crossref","first-page":"1037","DOI":"10.1016\/j.joen.2015.03.008","article-title":"Total and specific bacterial levels in the apical root canal system of teeth with post-treatment apical periodontitis","volume":"41","author":"Antunes","year":"2015","journal-title":"J Endod"},{"key":"10.1016\/j.joen.2023.11.005_bib14","doi-asserted-by":"crossref","first-page":"1040","DOI":"10.1016\/j.joen.2012.04.010","article-title":"One- versus two-visit endodontic treatment of teeth with apical periodontitis: a histobacteriologic study","volume":"38","author":"Vera","year":"2012","journal-title":"J Endod"},{"key":"10.1016\/j.joen.2023.11.005_bib15","doi-asserted-by":"crossref","first-page":"846","DOI":"10.1016\/j.joen.2020.03.017","article-title":"Cleaning, shaping, and disinfecting abilities of 2 instrument systems as evaluated by a correlative micro-computed tomographic and histobacteriologic approach","volume":"46","author":"Perez","year":"2020","journal-title":"J Endod"},{"key":"10.1016\/j.joen.2023.11.005_bib16","doi-asserted-by":"crossref","first-page":"493","DOI":"10.1016\/j.joen.2008.12.014","article-title":"Histologic investigation of root canal-treated teeth with apical periodontitis: a retrospective study from twenty-four patients","volume":"35","author":"Ricucci","year":"2009","journal-title":"J Endod"},{"key":"10.1016\/j.joen.2023.11.005_bib17","doi-asserted-by":"crossref","first-page":"220","DOI":"10.1016\/j.joen.2006.11.006","article-title":"How useful is root canal culturing in predicting treatment outcome?","volume":"33","author":"Sathorn","year":"2007","journal-title":"J Endod"},{"issue":"Suppl 1","key":"10.1016\/j.joen.2023.11.005_bib18","doi-asserted-by":"crossref","first-page":"46","DOI":"10.1111\/iej.13656","article-title":"A critical analysis of research methods and experimental models to study the root canal microbiome","volume":"55","author":"Siqueira","year":"2022","journal-title":"Int Endod J"},{"key":"10.1016\/j.joen.2023.11.005_bib19","doi-asserted-by":"crossref","first-page":"486","DOI":"10.1016\/j.joen.2008.12.022","article-title":"Bacterial community profiling of cryogenically ground samples from the apical and coronal root segments of teeth with apical periodontitis","volume":"35","author":"Alves","year":"2009","journal-title":"J Endod"},{"key":"10.1016\/j.joen.2023.11.005_bib20","doi-asserted-by":"crossref","first-page":"e0162887","DOI":"10.1371\/journal.pone.0162887","article-title":"Microbiome in the apical root canal system of teeth with post-treatment apical periodontitis","volume":"11","author":"Siqueira","year":"2016","journal-title":"PLoS One"},{"key":"10.1016\/j.joen.2023.11.005_bib21","doi-asserted-by":"crossref","first-page":"622","DOI":"10.1111\/iej.13893","article-title":"Microbiome in paired root apices and periapical lesions and its association with clinical signs in persistent apical periodontitis using next-generation sequencing","volume":"56","author":"Perez-Carrasco","year":"2023","journal-title":"Int Endod J"},{"key":"10.1016\/j.joen.2023.11.005_bib22","doi-asserted-by":"crossref","first-page":"189","DOI":"10.1186\/s12864-019-5474-y","article-title":"Microbiota in the apical root canal system of tooth with apical periodontitis","volume":"20","author":"Qian","year":"2019","journal-title":"BMC Genom"},{"key":"10.1016\/j.joen.2023.11.005_bib23","doi-asserted-by":"crossref","first-page":"1617","DOI":"10.1016\/j.joen.2010.07.001","article-title":"Apical root canal microbiota as determined by reverse-capture checkerboard analysis of cryogenically ground root samples from teeth with apical periodontitis","volume":"36","author":"R\u00f4\u00e7as","year":"2010","journal-title":"J Endod"},{"key":"10.1016\/j.joen.2023.11.005_bib24","doi-asserted-by":"crossref","first-page":"851","DOI":"10.1097\/01.don.0000164133.04548.26","article-title":"Enterococcus spp. in endodontically treated teeth with and without periradicular lesions","volume":"31","author":"Kaufman","year":"2005","journal-title":"J Endod"},{"key":"10.1016\/j.joen.2023.11.005_bib25","doi-asserted-by":"crossref","first-page":"722","DOI":"10.1016\/j.joen.2006.02.001","article-title":"Identification of Enterococcus faecalis in root-filled teeth with or without periradicular lesions by culture-dependent and -independent approaches","volume":"32","author":"Zoletti","year":"2006","journal-title":"J Endod"},{"key":"10.1016\/j.joen.2023.11.005_bib26","doi-asserted-by":"crossref","first-page":"1360","DOI":"10.1099\/jmm.0.018887-0","article-title":"Comparison of endodontic bacterial community structures in root-canal-treated teeth with or without apical periodontitis","volume":"59","author":"Zoletti","year":"2010","journal-title":"J Med Microbiol"},{"key":"10.1016\/j.joen.2023.11.005_bib27","doi-asserted-by":"crossref","first-page":"1009","DOI":"10.1016\/j.joen.2009.04.006","article-title":"Accuracy of periapical radiography and cone-beam computed tomography scans in diagnosing apical periodontitis using histopathological findings as a gold standard","volume":"35","author":"Paula-Silva","year":"2009","journal-title":"J Endod"},{"key":"10.1016\/j.joen.2023.11.005_bib28","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1111\/iej.12270","article-title":"Cone beam computed tomography in endodontics\u2013a review","volume":"48","author":"Patel","year":"2015","journal-title":"Int Endod J"},{"key":"10.1016\/j.joen.2023.11.005_bib29","doi-asserted-by":"crossref","first-page":"475","DOI":"10.1055\/s-0040-1722095","article-title":"Quality of root canal filling using a bioceramic sealer in oval canals: a three-dimensional analysis","volume":"15","author":"Penha da Silva","year":"2021","journal-title":"Eur J Dent"},{"key":"10.1016\/j.joen.2023.11.005_bib30","doi-asserted-by":"crossref","first-page":"1177","DOI":"10.1111\/iej.12269","article-title":"Micro-CT evaluation of root filling quality in oval-shaped canals","volume":"47","author":"Keles","year":"2014","journal-title":"Int Endod J"},{"key":"10.1016\/j.joen.2023.11.005_bib31","doi-asserted-by":"crossref","first-page":"e66","DOI":"10.1590\/1807-3107bor-2018.vol32.0066","article-title":"Root canal preparation using micro-computed tomography analysis: a literature review","volume":"32","author":"Sousa-Neto","year":"2018","journal-title":"Braz Oral Res"},{"key":"10.1016\/j.joen.2023.11.005_bib32","doi-asserted-by":"crossref","first-page":"280","DOI":"10.1016\/j.joen.2021.11.016","article-title":"Planned apical preparation using cone-beam computed tomographic measures: A micro-computed tomographic proof of concept in human cadavers","volume":"48","author":"Pacheco-Yanes","year":"2022","journal-title":"J Endod"},{"key":"10.1016\/j.joen.2023.11.005_bib33","doi-asserted-by":"crossref","first-page":"1695","DOI":"10.1016\/j.joen.2020.07.009","article-title":"Cone-beam computed tomographic and micro-computed tomographic evaluations of the root apexes of teeth with posttreatment apical periodontitis","volume":"46","author":"Villa-Machado","year":"2020","journal-title":"J Endod"},{"key":"10.1016\/j.joen.2023.11.005_bib34","doi-asserted-by":"crossref","first-page":"702","DOI":"10.1111\/j.1365-2591.2011.01989.x","article-title":"The detection of periapical pathosis using periapical radiography and cone beam computed tomography - part 1: pre-operative status","volume":"45","author":"Patel","year":"2012","journal-title":"Int Endod J"},{"key":"10.1016\/j.joen.2023.11.005_bib35","doi-asserted-by":"crossref","first-page":"382","DOI":"10.1016\/j.joen.2020.10.020","article-title":"Four-year outcome of nonsurgical root canal retreatment using cone-beam computed tomography: a prospective cohort study","volume":"47","author":"Zhang","year":"2021","journal-title":"J Endod"},{"key":"10.1016\/j.joen.2023.11.005_bib36","doi-asserted-by":"crossref","first-page":"1026","DOI":"10.1111\/j.1365-2591.2008.01484.x","article-title":"Outcome of secondary root canal treatment: a systematic review of the literature","volume":"41","author":"Ng","year":"2008","journal-title":"Int Endod J"},{"key":"10.1016\/j.joen.2023.11.005_bib37","doi-asserted-by":"crossref","first-page":"303","DOI":"10.1007\/s00248-010-9754-y","article-title":"Distribution of high bacterial taxa across the chronosequence of two alpine glacier forelands","volume":"61","author":"Philippot","year":"2011","journal-title":"Microb Ecol"},{"key":"10.1016\/j.joen.2023.11.005_bib38","doi-asserted-by":"crossref","first-page":"85","DOI":"10.1016\/S1079-2104(03)00353-6","article-title":"Polymerase chain reaction-based analysis of microorganisms associated with failed endodontic treatment","volume":"97","author":"Siqueira","year":"2004","journal-title":"Oral Surg Oral Med Oral Pathol Oral Radiol Endod"},{"key":"10.1016\/j.joen.2023.11.005_bib39","doi-asserted-by":"crossref","first-page":"1089","DOI":"10.1016\/j.joen.2019.05.021","article-title":"Outcome of endodontic retreatment using 2 root canal irrigants and influence of infection on healing as determined by a molecular method: a randomized clinical trial","volume":"45","author":"Zandi","year":"2019","journal-title":"J Endod"},{"key":"10.1016\/j.joen.2023.11.005_bib40","series-title":"Bacteriological studies of necrotic dental pulps [Odontological Dissertation no.7]","author":"Sundqvist","year":"1976"},{"key":"10.1016\/j.joen.2023.11.005_bib41","doi-asserted-by":"crossref","first-page":"3599","DOI":"10.1128\/JCM.00431-08","article-title":"Root canal microbiota of teeth with chronic apical periodontitis","volume":"46","author":"R\u00f4\u00e7as","year":"2008","journal-title":"J Clin Microbiol"},{"key":"10.1016\/j.joen.2023.11.005_bib42","doi-asserted-by":"crossref","first-page":"266","DOI":"10.1111\/j.1399-302X.2007.00355.x","article-title":"Cultivable bacteria in infected root canals as identified by 16S rRNA gene sequencing","volume":"22","author":"Siqueira","year":"2007","journal-title":"Oral Microbiol Immunol"},{"key":"10.1016\/j.joen.2023.11.005_bib43","doi-asserted-by":"crossref","first-page":"487","DOI":"10.1016\/j.joen.2022.01.012","article-title":"R\u00f4\u00e7as IN, Rachid CTC, Oliveira AGG, et\u00a0al. Root canal microbiome associated with asymptomatic apical periodontitis as determined by high-throughput sequencing","volume":"48","author":"Amaral","year":"2022","journal-title":"J Endod"},{"key":"10.1016\/j.joen.2023.11.005_bib44","doi-asserted-by":"crossref","first-page":"58","DOI":"10.1111\/j.1600-9657.1987.tb00543.x","article-title":"Healing of periapical lesions of pulpless teeth after endodontic treatment with controlled asepsis","volume":"3","author":"Bystr\u00f6m","year":"1987","journal-title":"Endod Dent Traumatol"},{"key":"10.1016\/j.joen.2023.11.005_bib45","series-title":"Treatment of Endodontic Infections","author":"Siqueira","year":"2022"},{"key":"10.1016\/j.joen.2023.11.005_bib46","doi-asserted-by":"crossref","first-page":"232","DOI":"10.1016\/j.tripleo.2004.10.008","article-title":"Streptococci from root canals in teeth with apical periodontitis receiving endodontic treatment","volume":"100","author":"Chavez de Paz","year":"2005","journal-title":"Oral Surg Oral Med Oral Pathol Oral Radiol Endod"},{"key":"10.1016\/j.joen.2023.11.005_bib47","doi-asserted-by":"crossref","first-page":"1721","DOI":"10.1128\/JCM.00531-12","article-title":"Characterization of microbiota of root canal-treated teeth with posttreatment disease","volume":"50","author":"R\u00f4\u00e7as","year":"2012","journal-title":"J Clin Microbiol"},{"key":"10.1016\/j.joen.2023.11.005_bib48","doi-asserted-by":"crossref","DOI":"10.3402\/jom.v6.23609","article-title":"Bacterial profiles of saliva in relation to diet, lifestyle factors, and socioeconomic status","volume":"6","author":"Belstrom","year":"2014","journal-title":"J Oral Microbiol"},{"key":"10.1016\/j.joen.2023.11.005_bib49","doi-asserted-by":"crossref","first-page":"5721","DOI":"10.1128\/JCM.43.11.5721-5732.2005","article-title":"Defining the normal bacterial flora of the oral cavity","volume":"43","author":"Aas","year":"2005","journal-title":"J Clin Microbiol"},{"key":"10.1016\/j.joen.2023.11.005_bib50","doi-asserted-by":"crossref","first-page":"6","DOI":"10.1111\/j.1365-2591.2007.01323.x","article-title":"Outcome of primary root canal treatment: systematic review of the literature - Part 2. Influence of clinical factors","volume":"41","author":"Ng","year":"2008","journal-title":"Int Endod J"},{"key":"10.1016\/j.joen.2023.11.005_bib51","doi-asserted-by":"crossref","first-page":"498","DOI":"10.1016\/S0099-2399(07)80180-4","article-title":"Factors affecting the long-term results of endodontic treatment","volume":"16","author":"Sjogren","year":"1990","journal-title":"J Endod"},{"key":"10.1016\/j.joen.2023.11.005_bib52","first-page":"330","article-title":"The apex: how critical is it?","volume":"42","author":"Simon","year":"1994","journal-title":"Gen Dent"}],"container-title":["Journal of Endodontics"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0099239923007239?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0099239923007239?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,9,25]],"date-time":"2025-09-25T19:55:08Z","timestamp":1758830108000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0099239923007239"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,2]]},"references-count":52,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2024,2]]}},"alternative-id":["S0099239923007239"],"URL":"https:\/\/doi.org\/10.1016\/j.joen.2023.11.005","relation":{},"ISSN":["0099-2399"],"issn-type":[{"value":"0099-2399","type":"print"}],"subject":[],"published":{"date-parts":[[2024,2]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Bacteriologic Conditions of the Apical Root Canal System of Teeth with and without Posttreatment Apical Periodontitis: A Correlative Multianalytical Approach","name":"articletitle","label":"Article Title"},{"value":"Journal of Endodontics","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.joen.2023.11.005","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2023 American Association of Endodontists.","name":"copyright","label":"Copyright"}]}}