{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,1]],"date-time":"2026-06-01T18:05:32Z","timestamp":1780337132769,"version":"3.54.1"},"reference-count":49,"publisher":"Elsevier BV","issue":"6","license":[{"start":{"date-parts":[[2026,6,1]],"date-time":"2026-06-01T00:00:00Z","timestamp":1780272000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2026,6,1]],"date-time":"2026-06-01T00:00:00Z","timestamp":1780272000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2026,3,16]],"date-time":"2026-03-16T00:00:00Z","timestamp":1773619200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001807","name":"FAPESP","doi-asserted-by":"publisher","award":["2022\/03093-9"],"award-info":[{"award-number":["2022\/03093-9"]}],"id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002322","name":"CAPES","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100002322","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["clinicalkey.com","clinicalkey.com.au","clinicalkey.es","clinicalkey.jp","jendodon.com","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Journal of Endodontics"],"published-print":{"date-parts":[[2026,6]]},"DOI":"10.1016\/j.joen.2026.03.003","type":"journal-article","created":{"date-parts":[[2026,3,11]],"date-time":"2026-03-11T23:44:17Z","timestamp":1773272657000},"page":"971-979.e3","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":1,"title":["Blood Metallomic and Histologic Organs Analysis in Induced Periapical Lesion Model: In Vivo Animal Study"],"prefix":"10.1016","volume":"52","author":[{"ORCID":"https:\/\/orcid.org\/0009-0003-3006-6406","authenticated-orcid":false,"given":"Anna C.N.","family":"Leutz","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mariliza C.V.","family":"da Costa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maritana M.","family":"Prodocimo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jennifer S.","family":"Pereira","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ana C.P.","family":"Janini","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Adriana J.","family":"Soares","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Brenda P.F.A.","family":"Gomes","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Talita","family":"Tartari","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lauter E.","family":"Pelepenko","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6244-2531","authenticated-orcid":false,"given":"Marina A.","family":"Marciano","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.joen.2026.03.003_bib1","doi-asserted-by":"crossref","DOI":"10.1016\/j.cbi.2022.110173","article-title":"Essential metals in health and disease","volume":"367","author":"Jomova","year":"2022","journal-title":"Chem Biol Interact"},{"key":"10.1016\/j.joen.2026.03.003_bib2","doi-asserted-by":"crossref","first-page":"120","DOI":"10.1016\/j.jinorgbio.2019.03.013","article-title":"The essential metals for humans: a brief overview","volume":"195","author":"Zoroddu","year":"2019","journal-title":"J Inorg Biochem"},{"key":"10.1016\/j.joen.2026.03.003_bib3","doi-asserted-by":"crossref","first-page":"1617","DOI":"10.1007\/s00784-015-1646-6","article-title":"Can apical periodontitis affect serum levels of CRP, IL-2, and IL-6 as well as induce pathological changes in remote organs?","volume":"20","author":"Zhang","year":"2016","journal-title":"Clin Oral Investig"},{"key":"10.1016\/j.joen.2026.03.003_bib4","doi-asserted-by":"crossref","first-page":"393","DOI":"10.1007\/s12011-019-01943-9","article-title":"Association between thyroid hormone status and trace elements in serum of patients with nodular goiter","volume":"196","author":"Kravchenko","year":"2020","journal-title":"Biol Trace Elem Res"},{"key":"10.1016\/j.joen.2026.03.003_bib5","doi-asserted-by":"crossref","first-page":"1424","DOI":"10.1002\/mas.21777","article-title":"Mass spectrometry-based \u201comics\u201d technologies for the study of gestational diabetes and the discovery of new biomarkers","volume":"42","author":"Roverso","year":"2023","journal-title":"Mass Spectrom Rev"},{"key":"10.1016\/j.joen.2026.03.003_bib6","doi-asserted-by":"crossref","first-page":"6","DOI":"10.3390\/biom11010006","article-title":"Metallomics analysis for assessment of toxic metal burdens in infants\/children and their mothers: early assessment and intervention are essential","volume":"11","author":"Yasuda","year":"2020","journal-title":"Biomolecules"},{"key":"10.1016\/j.joen.2026.03.003_bib7","doi-asserted-by":"crossref","first-page":"671","DOI":"10.1039\/C6JA00390G","article-title":"Quantitative bioimaging of Ca, Fe, Cu and Zn in breast cancer tissues by LA-ICP-MS","volume":"32","author":"Gonz\u00e1lez de Vega","year":"2017","journal-title":"J Anal At Spectrom"},{"key":"10.1016\/j.joen.2026.03.003_bib8","doi-asserted-by":"crossref","DOI":"10.1016\/j.ecoenv.2019.109479","article-title":"Evaluation of trace metals in thyroid tissues: comparative analysis with benign and malignant thyroid diseases","volume":"183","author":"Stojsavljevi\u0107","year":"2019","journal-title":"Ecotoxicol Environ Saf"},{"key":"10.1016\/j.joen.2026.03.003_bib9","doi-asserted-by":"crossref","first-page":"699","DOI":"10.1007\/s00775-023-02021-1","article-title":"Assessment of 13 essential and toxic trace elements in tumor and peritumoral brain tissues from human glioblastoma","volume":"28","author":"Zeng","year":"2023","journal-title":"J Biol Inorg Chem"},{"key":"10.1016\/j.joen.2026.03.003_bib10","doi-asserted-by":"crossref","DOI":"10.1016\/j.envres.2019.108599","article-title":"Human biomonitoring to evaluate exposure to toxic and essential trace elements during pregnancy. Part A: concentrations in maternal blood, urine and cord blood","volume":"177","author":"Bocca","year":"2019","journal-title":"Environ Res"},{"key":"10.1016\/j.joen.2026.03.003_bib11","doi-asserted-by":"crossref","DOI":"10.1016\/j.envres.2019.109108","article-title":"Human biomonitoring to evaluate exposure to toxic and essential trace elements during pregnancy. Part B: predictors of exposure","volume":"182","author":"Bocca","year":"2020","journal-title":"Environ Res"},{"key":"10.1016\/j.joen.2026.03.003_bib12","doi-asserted-by":"crossref","first-page":"58","DOI":"10.1007\/s12011-017-1137-5","article-title":"Statistical evaluation of trace metals, TSH and T4 in blood serum of thyroid disease patients in comparison with controls","volume":"183","author":"Hanif","year":"2018","journal-title":"Biol Trace Elem Res"},{"key":"10.1016\/j.joen.2026.03.003_bib13","doi-asserted-by":"crossref","first-page":"3482","DOI":"10.1007\/s12011-021-02945-2","article-title":"Elemental composition of pheochromocytoma resolved on solid\/adrenal tissue and whole blood level","volume":"200","author":"Jagodi\u0107","year":"2022","journal-title":"Biol Trace Elem Res"},{"key":"10.1016\/j.joen.2026.03.003_bib14","doi-asserted-by":"crossref","first-page":"841","DOI":"10.1046\/j.1525-1594.2000.06352.x","article-title":"Trace metals' abnormalities in hemodialysis patients: relationship with medications","volume":"24","author":"Lee","year":"2000","journal-title":"Artif Organs"},{"key":"10.1016\/j.joen.2026.03.003_bib15","doi-asserted-by":"crossref","first-page":"2572","DOI":"10.1007\/s12011-021-02862-4","article-title":"Profiling of circulatory elements reveals alteration of essential and toxic trace metals in Crohn's disease","volume":"200","author":"Stojsavljevi\u0107","year":"2022","journal-title":"Biol Trace Elem Res"},{"key":"10.1016\/j.joen.2026.03.003_bib16","doi-asserted-by":"crossref","DOI":"10.1016\/j.envres.2020.109538","article-title":"Significance of arsenic and lead in Hashimoto's thyroiditis demonstrated on thyroid tissue, blood, and urine samples","volume":"186","author":"Stojsavljevi\u0107","year":"2020","journal-title":"Environ Res"},{"key":"10.1016\/j.joen.2026.03.003_bib17","doi-asserted-by":"crossref","first-page":"3816","DOI":"10.1038\/s41598-020-60774-0","article-title":"Assessment of trace metal alterations in the blood, cerebrospinal fluid and tissue samples of patients with malignant brain tumors","volume":"10","author":"Stojsavljevi\u0107","year":"2020","journal-title":"Sci Rep"},{"key":"10.1016\/j.joen.2026.03.003_bib18","doi-asserted-by":"crossref","first-page":"30","DOI":"10.1111\/jre.12956","article-title":"Macro and trace elements signature of periodontitis in saliva: a systematic review with quality assessment of ionomics studies","volume":"57","author":"Baima","year":"2022","journal-title":"J Periodontal Res"},{"key":"10.1016\/j.joen.2026.03.003_bib19","doi-asserted-by":"crossref","first-page":"275","DOI":"10.1007\/s12011-016-0660-0","article-title":"Essential and toxic metals in oral fluid\u2014a potential role in the diagnosis of periodontal diseases","volume":"173","author":"Herman","year":"2016","journal-title":"Biol Trace Elem Res"},{"key":"10.1016\/j.joen.2026.03.003_bib20","doi-asserted-by":"crossref","first-page":"519","DOI":"10.3897\/pharmacia.70.e106478","article-title":"Changes of micro elements, activity of metalloenzymes-antioxidants and metal-dependent enzymes in the blood serum of patients with periodontitis influenced by complex non-surgical treatment","volume":"70","author":"Melnychuk","year":"2023","journal-title":"Pharmacia"},{"key":"10.1016\/j.joen.2026.03.003_bib21","doi-asserted-by":"crossref","first-page":"605","DOI":"10.1111\/j.1600-0714.2009.00788.x","article-title":"Production of proinflammatory and immunoregulatory cytokines by inflammatory cells from periapical lesions in culture","volume":"38","author":"Gazivoda","year":"2009","journal-title":"J Oral Pathol Med"},{"issue":"Suppl 1","key":"10.1016\/j.joen.2026.03.003_bib22","doi-asserted-by":"crossref","DOI":"10.1590\/1807-3107bor-2018.vol32.0069","article-title":"Etiologic role of root canal infection in apical periodontitis and its relationship with clinical symptomatology","volume":"32","author":"Gomes","year":"2018","journal-title":"Braz Oral Res"},{"key":"10.1016\/j.joen.2026.03.003_bib23","doi-asserted-by":"crossref","first-page":"249","DOI":"10.1111\/j.1365-2591.2006.01099.x","article-title":"On the causes of persistent apical periodontitis: a review","volume":"39","author":"Nair","year":"2006","journal-title":"Int Endod J"},{"key":"10.1016\/j.joen.2026.03.003_bib24","doi-asserted-by":"crossref","first-page":"2204","DOI":"10.2174\/1381612822666160216145107","article-title":"Interrelationship between periapical lesion and systemic metabolic disorders","volume":"22","author":"Sasaki","year":"2016","journal-title":"Curr Pharm Des"},{"key":"10.1016\/j.joen.2026.03.003_bib25","doi-asserted-by":"crossref","first-page":"1594","DOI":"10.1111\/iej.14274","article-title":"Metallomic characterization of induced periapical lesions\u2014in vivo study","volume":"58","author":"Pereira","year":"2025","journal-title":"Int Endod J"},{"key":"10.1016\/j.joen.2026.03.003_bib26","doi-asserted-by":"crossref","first-page":"1358","DOI":"10.1177\/0022034516660509","article-title":"Association of endodontic lesions with coronary artery disease","volume":"95","author":"Liljestrand","year":"2016","journal-title":"J Dent Res"},{"key":"10.1016\/j.joen.2026.03.003_bib27","doi-asserted-by":"crossref","first-page":"1395","DOI":"10.1007\/s00784-017-2222-z","article-title":"Endodontic infections increase leukocyte and lymphocyte levels in the blood","volume":"22","author":"Samuel","year":"2018","journal-title":"Clin Oral Investig"},{"key":"10.1016\/j.joen.2026.03.003_bib28","doi-asserted-by":"crossref","first-page":"1","DOI":"10.7150\/ijms.6663","article-title":"Influence of endodontic treatment on systemic oxidative stress","volume":"11","author":"Inchingolo","year":"2013","journal-title":"Int J Med Sci"},{"key":"10.1016\/j.joen.2026.03.003_bib29","doi-asserted-by":"crossref","first-page":"747","DOI":"10.1016\/j.joen.2016.01.022","article-title":"Multiple apical periodontitis influences serum levels of cytokines and nitric oxide","volume":"42","author":"Cintra","year":"2016","journal-title":"J Endod"},{"key":"10.1016\/j.joen.2026.03.003_bib30","doi-asserted-by":"crossref","first-page":"848","DOI":"10.1111\/iej.13477","article-title":"PRIASE 2021 guidelines for reporting animal studies in endodontology: a consensus-based development","volume":"54","author":"Nagendrababu","year":"2021","journal-title":"Int Endod J"},{"key":"10.1016\/j.joen.2026.03.003_bib31","first-page":"588","article-title":"Local injection of carrageenan accelerates orthodontic tooth movement: a preliminary experimental animal study","volume":"15","author":"Kavoli","year":"2017","journal-title":"Int Orthod"},{"key":"10.1016\/j.joen.2026.03.003_bib32","doi-asserted-by":"crossref","first-page":"101","DOI":"10.1055\/s-0040-1715986","article-title":"Effect of tricalcium silicate on direct pulp capping: experimental study in rats","volume":"15","author":"Chicarelli","year":"2021","journal-title":"Eur J Dent"},{"key":"10.1016\/j.joen.2026.03.003_bib33","doi-asserted-by":"crossref","first-page":"643","DOI":"10.1097\/00004770-200209000-00004","article-title":"Periapical lesion development in rats inhibited by dexamethasone","volume":"28","author":"Metzger","year":"2002","journal-title":"J Endod"},{"key":"10.1016\/j.joen.2026.03.003_bib34","doi-asserted-by":"crossref","first-page":"371","DOI":"10.1590\/S1678-77572006000500013","article-title":"Development of periradicular lesions in normal and diabetic rats","volume":"14","author":"Armada-Dias","year":"2006","journal-title":"J Appl Oral Sci"},{"key":"10.1016\/j.joen.2026.03.003_bib35","doi-asserted-by":"crossref","first-page":"161","DOI":"10.1111\/iej.12612","article-title":"Influence of oestrogen deficiency on the development of apical periodontitis","volume":"50","author":"Brasil","year":"2017","journal-title":"Int Endod J"},{"key":"10.1016\/j.joen.2026.03.003_bib36","doi-asserted-by":"crossref","first-page":"600","DOI":"10.1016\/j.joen.2020.12.015","article-title":"Influence of a high-fat diet in the progression of apical periodontitis","volume":"47","author":"Brasil","year":"2021","journal-title":"J Endod"},{"key":"10.1016\/j.joen.2026.03.003_bib37","doi-asserted-by":"crossref","first-page":"224","DOI":"10.1016\/j.jtemb.2018.04.002","article-title":"Kinetics and tissue distribution of bismuth, tin and lead after implantation of miniature shotgun alloy pellets in rats","volume":"48","author":"Skaug","year":"2018","journal-title":"J Trace Elem Med Biol"},{"key":"10.1016\/j.joen.2026.03.003_bib38","doi-asserted-by":"crossref","first-page":"115","DOI":"10.33176\/AACB-19-00024","article-title":"Inductively coupled plasma mass spectrometry: introduction to analytical aspects","volume":"40","author":"Wilschefski","year":"2019","journal-title":"Clin Biochem Rev"},{"issue":"Suppl 1","key":"10.1016\/j.joen.2026.03.003_bib39","doi-asserted-by":"crossref","first-page":"S49","DOI":"10.1016\/j.ymgme.2010.02.020","article-title":"Creatine metabolism and the urea cycle","volume":"100","author":"Brosnan","year":"2010","journal-title":"Mol Genet Metab"},{"key":"10.1016\/j.joen.2026.03.003_bib40","doi-asserted-by":"crossref","first-page":"25","DOI":"10.1046\/j.1365-2761.1999.00134.x","article-title":"Histopathology in fish: proposal for a protocol to assess aquatic pollution","volume":"22","author":"Bernet","year":"1999","journal-title":"J Fish Dis"},{"key":"10.1016\/j.joen.2026.03.003_bib41","doi-asserted-by":"crossref","first-page":"108","DOI":"10.1016\/j.ecoenv.2012.11.002","article-title":"Risks of waterborne copper exposure to a cultivated freshwater Neotropical catfish (Rhamdia quelen)","volume":"88","author":"Mela","year":"2013","journal-title":"Ecotoxicol Environ Saf"},{"key":"10.1016\/j.joen.2026.03.003_bib42","doi-asserted-by":"crossref","first-page":"31675","DOI":"10.1007\/s11356-021-18201-y","article-title":"Disordered serum essential element levels are associated with increased risk of kidney tumors","volume":"29","author":"Zhang","year":"2022","journal-title":"Environ Sci Pollut Res Int"},{"key":"10.1016\/j.joen.2026.03.003_bib43","doi-asserted-by":"crossref","first-page":"1480","DOI":"10.1016\/j.joen.2008.09.003","article-title":"The potential role of suppressors of cytokine signaling in the attenuation of inflammatory reaction and alveolar bone loss associated with apical periodontitis","volume":"34","author":"Menezes","year":"2008","journal-title":"J Endod"},{"key":"10.1016\/j.joen.2026.03.003_bib44","doi-asserted-by":"crossref","first-page":"289","DOI":"10.1590\/1981-8637201400030000092561","article-title":"Role of endotoxin in the etiology of periapical lesions: molecular mechanisms involved in endotoxin's recognition and cell activation","volume":"62","author":"Lucisano","year":"2014","journal-title":"RGO Rev Ga\u00fach Odontol"},{"key":"10.1016\/j.joen.2026.03.003_bib45","doi-asserted-by":"crossref","first-page":"173","DOI":"10.1080\/00016357.2018.1521005","article-title":"Inflammatory profile of chronic apical periodontitis: a literature review","volume":"77","author":"Braz-Silva","year":"2019","journal-title":"Acta Odontol Scand"},{"key":"10.1016\/j.joen.2026.03.003_bib46","doi-asserted-by":"crossref","DOI":"10.3389\/fimmu.2022.949102","article-title":"Macrophages in periapical lesions: potential roles and future directions","volume":"13","author":"Song","year":"2022","journal-title":"Front Immunol"},{"issue":"Suppl 2","key":"10.1016\/j.joen.2026.03.003_bib47","doi-asserted-by":"crossref","first-page":"219","DOI":"10.1111\/iej.13789","article-title":"Impact of systemic health on treatment outcomes in endodontics","volume":"56","author":"Segura-Egea","year":"2023","journal-title":"Int Endod J"},{"key":"10.1016\/j.joen.2026.03.003_bib48","doi-asserted-by":"crossref","first-page":"1353","DOI":"10.1111\/iej.13519","article-title":"Inflammatory profile of apical periodontitis associated with liver fibrosis in rats: histological and immunohistochemical analysis","volume":"54","author":"Cantiga-Silva","year":"2021","journal-title":"Int Endod J"},{"key":"10.1016\/j.joen.2026.03.003_bib49","doi-asserted-by":"crossref","first-page":"1472","DOI":"10.1016\/j.joen.2023.08.012","article-title":"Association of apical periodontitis with different stages of chronic kidney disease measured by glomerular filtration rate and systemic markers: an observational study","volume":"49","author":"Lamba","year":"2023","journal-title":"J Endod"}],"container-title":["Journal of Endodontics"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0099239926001275?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0099239926001275?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,5,31]],"date-time":"2026-05-31T20:31:56Z","timestamp":1780259516000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0099239926001275"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,6]]},"references-count":49,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2026,6]]}},"alternative-id":["S0099239926001275"],"URL":"https:\/\/doi.org\/10.1016\/j.joen.2026.03.003","relation":{},"ISSN":["0099-2399"],"issn-type":[{"value":"0099-2399","type":"print"}],"subject":[],"published":{"date-parts":[[2026,6]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Blood Metallomic and Histologic Organs Analysis in Induced Periapical Lesion Model: In Vivo Animal Study","name":"articletitle","label":"Article Title"},{"value":"Journal of Endodontics","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.joen.2026.03.003","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2026 The Authors. Published by Elsevier Inc. on behalf of American Association of Endodontists.","name":"copyright","label":"Copyright"}]}}