{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,26]],"date-time":"2026-06-26T20:49:45Z","timestamp":1782506985023,"version":"3.54.5"},"reference-count":35,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2020,7,1]],"date-time":"2020-07-01T00:00:00Z","timestamp":1593561600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2020,7,1]],"date-time":"2020-07-01T00:00:00Z","timestamp":1593561600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2020,2,18]],"date-time":"2020-02-18T00:00:00Z","timestamp":1581984000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Journal of Orthopaedic Translation"],"published-print":{"date-parts":[[2020,7]]},"DOI":"10.1016\/j.jot.2020.02.009","type":"journal-article","created":{"date-parts":[[2020,3,28]],"date-time":"2020-03-28T08:48:26Z","timestamp":1585385306000},"page":"29-37","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":36,"special_numbering":"C","title":["Wnt10b-overexpressing umbilical cord mesenchymal stem cells promote critical size rat calvarial defect healing by enhanced osteogenesis and VEGF-mediated angiogenesis"],"prefix":"10.1016","volume":"23","author":[{"given":"Yong","family":"Liu","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiarui","family":"Fang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Quan","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoguang","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6612-6218","authenticated-orcid":false,"given":"Yulin","family":"Cao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wei","family":"Chen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zengwu","family":"Shao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shuhua","family":"Yang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dongcheng","family":"Wu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Man","family":"Hung","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yingze","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8031-0380","authenticated-orcid":false,"given":"Wei","family":"Tong","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hongtao","family":"Tian","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"issue":"Suppl 2","key":"10.1016\/j.jot.2020.02.009_bib1","article-title":"Prevalence of long-bone non-unions","volume":"38","author":"Tzioupis","year":"2007","journal-title":"Injury"},{"issue":"18","key":"10.1016\/j.jot.2020.02.009_bib2","doi-asserted-by":"crossref","first-page":"1748","DOI":"10.1056\/NEJMra1716145","article-title":"Stem cells in the treatment of disease","volume":"380","author":"Blau","year":"2019","journal-title":"N\u00a0Engl J Med"},{"key":"10.1016\/j.jot.2020.02.009_bib3","doi-asserted-by":"crossref","first-page":"12","DOI":"10.1186\/1478-811X-9-12","article-title":"Different populations and sources of human mesenchymal stem cells (MSC): a comparison of adult and neonatal tissue-derived MSC","volume":"9","author":"Hass","year":"2011","journal-title":"Cell Commun Signal"},{"issue":"6","key":"10.1016\/j.jot.2020.02.009_bib4","doi-asserted-by":"crossref","first-page":"1384","DOI":"10.1634\/stemcells.2006-0709","article-title":"Comparison of proliferative and multilineage differentiation potential of human mesenchymal stem cells derived from umbilical cord and bone marrow","volume":"25","author":"Baksh","year":"2007","journal-title":"Stem Cell"},{"issue":"3","key":"10.1016\/j.jot.2020.02.009_bib5","doi-asserted-by":"crossref","first-page":"339","DOI":"10.3727\/096368915X686841","article-title":"Human umbilical cord mesenchymal stem cells: a new era for stem cell therapy","volume":"24","author":"Ding","year":"2015","journal-title":"Cell Transplant"},{"issue":"10","key":"10.1016\/j.jot.2020.02.009_bib6","doi-asserted-by":"crossref","first-page":"1192","DOI":"10.1161\/CIRCRESAHA.117.310712","volume":"121","author":"Bartolucci","year":"2017","journal-title":"Circ Res"},{"issue":"4","key":"10.1016\/j.jot.2020.02.009_bib7","first-page":"P291","article-title":"A phase I trial of parenchymal injection of umbilical cord stem cells in Alzheimer\u2019s disease","volume":"9","author":"Sang","year":"2013","journal-title":"Alzheimer\u2019s & Dementia"},{"issue":"3","key":"10.1016\/j.jot.2020.02.009_bib8","doi-asserted-by":"crossref","first-page":"215","DOI":"10.1002\/sctm.18-0053","article-title":"Umbilical cord-derived mesenchymal stromal cells (MSCs) for knee osteoarthritis: repeated MSC dosing is superior to a single MSC dose and to hyaluronic acid in a controlled randomized phase I\/II trial","volume":"8","author":"Matas","year":"2019","journal-title":"Stem Cell Transl Med"},{"issue":"2","key":"10.1016\/j.jot.2020.02.009_bib9","doi-asserted-by":"crossref","DOI":"10.1111\/cpr.12570","article-title":"Exosomes from human umbilical cord mesenchymal stem cells enhance fracture healing through HIF-1\u03b1-mediated promotion of angiogenesis in a rat model of stabilized fracture","volume":"52","author":"Zhang","year":"2019","journal-title":"Cell Prolif"},{"issue":"11","key":"10.1016\/j.jot.2020.02.009_bib10","first-page":"4954","article-title":"Effects of human umbilical cord mesenchymal stem cells-derived exosomes on fracture healing in rats through the Wnt signaling pathway","volume":"23","author":"Zhou","year":"2019","journal-title":"Eur Rev Med Pharmacol Sci"},{"issue":"13","key":"10.1016\/j.jot.2020.02.009_bib11","doi-asserted-by":"crossref","first-page":"1570","DOI":"10.1089\/scd.2014.0490","article-title":"Transplanted umbilical cord mesenchymal stem cells modify the in vivo microenvironment enhancing angiogenesis and leading to bone regeneration","volume":"24","author":"Todeschi","year":"2015","journal-title":"Stem Cell Dev"},{"key":"10.1016\/j.jot.2020.02.009_bib12","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1016\/j.ijscr.2017.03.002","article-title":"Modified Masquelet technique using allogeneic umbilical cord-derived mesenchymal stem cells for infected non-union femoral shaft fracture with a 12 cm bone defect: a case report","volume":"34","author":"Dilogo","year":"2017","journal-title":"Int J Surg Case Rep"},{"issue":"34","key":"10.1016\/j.jot.2020.02.009_bib13","doi-asserted-by":"crossref","first-page":"35503","DOI":"10.1074\/jbc.M402937200","article-title":"Wnt10b inhibits development of white and brown adipose tissues","volume":"279","author":"Longo","year":"2004","journal-title":"J\u00a0Biol Chem"},{"issue":"9","key":"10.1016\/j.jot.2020.02.009_bib14","doi-asserted-by":"crossref","first-page":"3324","DOI":"10.1073\/pnas.0408742102","article-title":"Regulation of osteoblastogenesis and bone mass by Wnt10b","volume":"102","author":"Bennett","year":"2005","journal-title":"Proc Natl Acad Sci U S A"},{"issue":"6205","key":"10.1016\/j.jot.2020.02.009_bib15","doi-asserted-by":"crossref","first-page":"1248012","DOI":"10.1126\/science.1248012","article-title":"Stem cell signaling. An integral program for tissue renewal and regeneration: Wnt signaling and stem cell control","volume":"346","author":"Clevers","year":"2014","journal-title":"Science"},{"issue":"1","key":"10.1016\/j.jot.2020.02.009_bib16","doi-asserted-by":"crossref","first-page":"25","DOI":"10.1242\/dev.128.1.25","article-title":"Mouse Wnt receptor gene Fzd5 is essential for yolk sac and placental angiogenesis","volume":"128","author":"Ishikawa","year":"2001","journal-title":"Development"},{"key":"10.1016\/j.jot.2020.02.009_bib17","doi-asserted-by":"crossref","first-page":"2673","DOI":"10.2147\/IJN.S131251","article-title":"Comparative study of porous hydroxyapatite\/chitosan and whitlockite\/chitosan scaffolds for bone regeneration in calvarial defects","volume":"12","author":"Zhou","year":"2017","journal-title":"Int J Nanomed"},{"issue":"10","key":"10.1016\/j.jot.2020.02.009_bib18","doi-asserted-by":"crossref","first-page":"1918","DOI":"10.1038\/nprot.2012.113","article-title":"Evaluation of bone regeneration using the rat critical size calvarial defect","volume":"7","author":"Spicer","year":"2012","journal-title":"Nat Protoc"},{"issue":"5","key":"10.1016\/j.jot.2020.02.009_bib19","doi-asserted-by":"crossref","first-page":"931","DOI":"10.1016\/j.bone.2008.06.019","article-title":"Dual delivery of an angiogenic and an osteogenic growth factor for bone regeneration in a critical size defect model","volume":"43","author":"Patel","year":"2008","journal-title":"Bone"},{"issue":"4","key":"10.1016\/j.jot.2020.02.009_bib20","doi-asserted-by":"crossref","first-page":"1615","DOI":"10.1007\/s10616-014-9818-9","article-title":"Effects of hypoxia on proliferation of human cord blood-derived mesenchymal stem cells","volume":"68","author":"Peng","year":"2016","journal-title":"Cytotechnology"},{"issue":"7","key":"10.1016\/j.jot.2020.02.009_bib21","doi-asserted-by":"crossref","first-page":"2182","DOI":"10.1002\/stem.2020","article-title":"Novel pathway for hypoxia-induced proliferation and migration in human mesenchymal stem cells: involvement of HIF-1\u03b1, FASN, and mTORC1","volume":"33","author":"Lee","year":"2015","journal-title":"Stem Cell (Dayton, Ohio)"},{"issue":"1","key":"10.1016\/j.jot.2020.02.009_bib22","doi-asserted-by":"crossref","first-page":"191","DOI":"10.1002\/term.2395","volume":"12","author":"Chen","year":"2018","journal-title":"J\u00a0Tissue Eng Regen Med"},{"issue":"29","key":"10.1016\/j.jot.2020.02.009_bib23","doi-asserted-by":"crossref","first-page":"E3893","DOI":"10.1073\/pnas.1501759112","article-title":"PDGFB-based stem cell gene therapy increases bone strength in the mouse","volume":"112","author":"Chen","year":"2015","journal-title":"Proc Natl Acad Sci U S A"},{"issue":"7","key":"10.1016\/j.jot.2020.02.009_bib24","doi-asserted-by":"crossref","first-page":"906","DOI":"10.1089\/hum.2018.054","article-title":"Lentiviral gene therapy for bone repair using human umbilical cord blood-derived mesenchymal stem cells","volume":"30","author":"Bougioukli","year":"2019","journal-title":"Hum Gene Ther"},{"issue":"1","key":"10.1016\/j.jot.2020.02.009_bib25","doi-asserted-by":"crossref","first-page":"36","DOI":"10.1111\/cpr.12236","article-title":"Overexpression of CD61 promotes hUC-MSC differentiation into male germ-like cells","volume":"49","author":"Li","year":"2016","journal-title":"Cell Prolif"},{"issue":"6","key":"10.1016\/j.jot.2020.02.009_bib26","article-title":"Overexpression of IL-10 enhances the efficacy of human umbilical-cord-derived mesenchymal stromal cells in pneumosepsis","volume":"8","author":"Jerkic","year":"2019","journal-title":"J\u00a0Clin Med"},{"issue":"1","key":"10.1016\/j.jot.2020.02.009_bib27","doi-asserted-by":"crossref","first-page":"4","DOI":"10.1002\/jgm.477","article-title":"Local ex vivo gene therapy with bone marrow stromal cells expressing human BMP4 promotes endosteal bone formation in mice","volume":"6","author":"Zhang","year":"2004","journal-title":"J\u00a0Gene Med"},{"issue":"4","key":"10.1016\/j.jot.2020.02.009_bib28","doi-asserted-by":"crossref","first-page":"933","DOI":"10.1016\/j.stemcr.2017.03.002","article-title":"Postnatal calvarial skeletal stem cells expressing PRX1 reside exclusively in the calvarial sutures and are required for bone regeneration","volume":"8","author":"Wilk","year":"2017","journal-title":"Stem Cell Rep"},{"issue":"3","key":"10.1016\/j.jot.2020.02.009_bib29","doi-asserted-by":"crossref","first-page":"520","DOI":"10.1002\/jbmr.3626","article-title":"Periosteal mesenchymal progenitor dysfunction and extraskeletally-derived fibrosis contribute to atrophic fracture nonunion","volume":"34","author":"Wang","year":"2019","journal-title":"J\u00a0Bone Miner Res"},{"issue":"9","key":"10.1016\/j.jot.2020.02.009_bib30","doi-asserted-by":"crossref","first-page":"804","DOI":"10.1161\/CIRCRESAHA.115.306886","article-title":"Wnt10b gain-of-function improves cardiac repair by arteriole formation and attenuation of fibrosis","volume":"117","author":"Paik","year":"2015","journal-title":"Circ Res"},{"issue":"2","key":"10.1016\/j.jot.2020.02.009_bib31","doi-asserted-by":"crossref","first-page":"509","DOI":"10.1172\/JCI82585","article-title":"Osteoblast-derived VEGF regulates osteoblast differentiation and bone formation during bone repair","volume":"126","author":"Hu","year":"2016","journal-title":"J\u00a0Clin Invest"},{"issue":"5","key":"10.1016\/j.jot.2020.02.009_bib32","doi-asserted-by":"crossref","first-page":"739","DOI":"10.1016\/j.devcel.2005.03.016","article-title":"Wnt\/beta-catenin signaling in mesenchymal progenitors controls osteoblast and chondrocyte differentiation during vertebrate skeletogenesis","volume":"8","author":"Day","year":"2005","journal-title":"Dev Cell"},{"issue":"6","key":"10.1016\/j.jot.2020.02.009_bib33","doi-asserted-by":"crossref","first-page":"999","DOI":"10.1016\/j.cellsig.2007.11.011","article-title":"Wnt signaling and skeletal development","volume":"20","author":"Liu","year":"2008","journal-title":"Cell Signal"},{"issue":"1","key":"10.1016\/j.jot.2020.02.009_bib34","doi-asserted-by":"crossref","first-page":"26","DOI":"10.3109\/14653249.2011.605119","article-title":"Comparison of chemokine and receptor gene expression between Wharton\u2019s jelly and bone marrow-derived mesenchymal stromal cells","volume":"14","author":"Balasubramanian","year":"2012","journal-title":"Cytotherapy"},{"issue":"9","key":"10.1016\/j.jot.2020.02.009_bib35","doi-asserted-by":"crossref","first-page":"3101","DOI":"10.1172\/JCI61209","article-title":"Intracellular VEGF regulates the balance between osteoblast and adipocyte differentiation","volume":"122","author":"Liu","year":"2012","journal-title":"J\u00a0Clin Invest"}],"container-title":["Journal of Orthopaedic Translation"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S2214031X20300188?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S2214031X20300188?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,11,1]],"date-time":"2025-11-01T10:42:28Z","timestamp":1761993748000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S2214031X20300188"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,7]]},"references-count":35,"alternative-id":["S2214031X20300188"],"URL":"https:\/\/doi.org\/10.1016\/j.jot.2020.02.009","relation":{},"ISSN":["2214-031X"],"issn-type":[{"value":"2214-031X","type":"print"}],"subject":[],"published":{"date-parts":[[2020,7]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Wnt10b-overexpressing umbilical cord mesenchymal stem cells promote critical size rat calvarial defect healing by enhanced osteogenesis and VEGF-mediated angiogenesis","name":"articletitle","label":"Article Title"},{"value":"Journal of Orthopaedic Translation","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.jot.2020.02.009","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2020 The Author(s). Published by Elsevier (Singapore) Pte Ltd on behalf of Chinese Speaking Orthopaedic Society.","name":"copyright","label":"Copyright"}]}}