{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T17:23:36Z","timestamp":1790011416763,"version":"4.0.1"},"reference-count":47,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2017,10,1]],"date-time":"2017-10-01T00:00:00Z","timestamp":1506816000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"}],"funder":[{"name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico (CNPq)"},{"name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de Minas Gerais (FAPEMIG)"},{"name":"Coordena\u00e7\u00e3o de Aperfei\u00e7oamento de Pessoal de N\u00edvel Superior (CAPES)"}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Journal of Pharmaceutical and Biomedical Analysis"],"published-print":{"date-parts":[[2017,10]]},"DOI":"10.1016\/j.jpba.2017.07.050","type":"journal-article","created":{"date-parts":[[2017,8,9]],"date-time":"2017-08-09T17:45:38Z","timestamp":1502300738000},"page":"838-844","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":14,"special_numbering":"C","title":["Development of direct assays for Toxoplasma gondii and its use in genomic DNA sample"],"prefix":"10.1016","volume":"145","author":[{"given":"L\u00edvia M.","family":"Alves","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vin\u00edcius R.","family":"Rodovalho","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ana C.H.","family":"Castro","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M\u00e1rcia A.R.","family":"Freitas","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Caroline M.","family":"Mota","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tiago W.P.","family":"Mineo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jos\u00e9 R.","family":"Mineo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jo\u00e3o M.","family":"Madurro","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ana G.","family":"Brito-Madurro","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.jpba.2017.07.050_bib0005","doi-asserted-by":"crossref","first-page":"1217","DOI":"10.1016\/S0020-7519(00)00124-7","article-title":"Toxoplasma gondii: from animals to humans","volume":"30","author":"Tentera","year":"2000","journal-title":"Int. J. Parasitol."},{"key":"10.1016\/j.jpba.2017.07.050_bib0010","doi-asserted-by":"crossref","first-page":"828","DOI":"10.1111\/apha.12458","article-title":"Strain hypothesis of Toxoplasma gondii infection on the outcome of human diseases","volume":"213","author":"Xiao","year":"2015","journal-title":"Acta Physiol."},{"key":"10.1016\/j.jpba.2017.07.050_bib0015","doi-asserted-by":"crossref","first-page":"815","DOI":"10.1586\/eri.12.58","article-title":"Toxoplasmosis in the fetus and newborn: an update on prevalence, diagnosis and treatment","volume":"10","author":"Moncada","year":"2012","journal-title":"Expert Rev. Anti Infect. Ther."},{"key":"10.1016\/j.jpba.2017.07.050_bib0020","doi-asserted-by":"crossref","first-page":"292","DOI":"10.1186\/s13071-015-0902-6","article-title":"Diagnosis of toxoplasmosis and typing of Toxoplasma gondii","volume":"8","author":"Liu","year":"2015","journal-title":"Parasite Vectors"},{"key":"10.1016\/j.jpba.2017.07.050_bib0025","doi-asserted-by":"crossref","first-page":"701","DOI":"10.1016\/S0956-5663(03)00265-3","article-title":"A piezoelectric immunoagglutination assay for Toxoplasma gondii antibodies using gold nanoparticles","volume":"19","author":"Wang","year":"2004","journal-title":"\u200eBiosens. Bioelectron."},{"issue":"7","key":"10.1016\/j.jpba.2017.07.050_bib0030","doi-asserted-by":"crossref","first-page":"1491","DOI":"10.1007\/s00216-005-3350-x","article-title":"Enzyme-catalyzed amplified immunoassay for the detection of Toxoplasma gondii \u2212specific IgG using Faradaic impedance spectroscopy, CV and QCM","volume":"382","author":"Ding","year":"2005","journal-title":"Anal. Bioanal. Chem."},{"key":"10.1016\/j.jpba.2017.07.050_bib0035","doi-asserted-by":"crossref","first-page":"481","DOI":"10.1016\/j.colsurfb.2012.07.021","article-title":"A novel immunosensor for detecting toxoplasma gondii-specific IgM based on goldmag nanoparticles and graphene sheets","volume":"101","author":"Jiang","year":"2013","journal-title":"Colloids Surf. B"},{"key":"10.1016\/j.jpba.2017.07.050_bib0040","doi-asserted-by":"crossref","first-page":"8354","DOI":"10.1021\/ac401755s","article-title":"Dual-aptamer-based biosensing of toxoplasma antibody","volume":"85","author":"Luo","year":"2013","journal-title":"Anal. Chem."},{"key":"10.1016\/j.jpba.2017.07.050_bib0045","doi-asserted-by":"crossref","first-page":"130","DOI":"10.9734\/IJBCRR\/2015\/15254","article-title":"Fluorescent detection of specific DNA sequences related to Toxoplasma gondii based on magnetic fluorescent nanoparticles Fe3O4\/CdTe biosensor","volume":"6","author":"He","year":"2015","journal-title":"Int. J. Biochem. Res. Rev."},{"key":"10.1016\/j.jpba.2017.07.050_bib0050","doi-asserted-by":"crossref","first-page":"244","DOI":"10.1016\/j.talanta.2015.11.071","article-title":"Voltammetric detection of sequence-selective DNA hybridization related to Toxoplasma gondii in PCR amplicons","volume":"149","author":"Gokce","year":"2016","journal-title":"Talanta"},{"key":"10.1016\/j.jpba.2017.07.050_bib0055","doi-asserted-by":"crossref","first-page":"321","DOI":"10.1016\/j.ab.2004.06.021","article-title":"Voltammetric determination of all DNA nucleotides","volume":"332","author":"Oliveira-Brett","year":"2004","journal-title":"Anal. Biochem."},{"key":"10.1016\/j.jpba.2017.07.050_bib0060","doi-asserted-by":"crossref","first-page":"6246","DOI":"10.1016\/S1452-3981(23)10885-6","article-title":"Graphite electrodes modified with poly(3-hydroxybenzoic acid) for oligonucleotides sensors","volume":"9","author":"Ferreira","year":"2014","journal-title":"Int. J. Electrochem. Sci."},{"key":"10.1016\/j.jpba.2017.07.050_bib0065","doi-asserted-by":"crossref","first-page":"1192","DOI":"10.1038\/nbt873","article-title":"Electrochemical DNA sensors","volume":"21","author":"Drummond","year":"2003","journal-title":"Nat. Biotechnol."},{"key":"10.1016\/j.jpba.2017.07.050_bib0070","doi-asserted-by":"crossref","first-page":"3427","DOI":"10.1021\/cr200303p","article-title":"Electrochemistry of nucleic acids","volume":"112","author":"Pale\u010dek","year":"2012","journal-title":"Chem. Rev."},{"key":"10.1016\/j.jpba.2017.07.050_bib0075","doi-asserted-by":"crossref","first-page":"77","DOI":"10.1016\/j.colsurfb.2011.10.042","article-title":"Single-walled carbon nanotubes-polymer modified graphite electrodes for DNA hybridization","volume":"91","author":"Muti","year":"2012","journal-title":"Colloids Surf. B"},{"key":"10.1016\/j.jpba.2017.07.050_bib0080","doi-asserted-by":"crossref","first-page":"1173","DOI":"10.3390\/ijms9071173","article-title":"Electrochemical Investigation of oligonucleotide-DNA hybridization on poly(4-methoxyphenethylamine)","volume":"9","author":"Silva","year":"2008","journal-title":"Int. J. Mol. Sci."},{"key":"10.1016\/j.jpba.2017.07.050_bib0085","doi-asserted-by":"crossref","first-page":"e115","DOI":"10.1093\/nar\/gks596","article-title":"Primer3\u2013new capabilities and interfaces","volume":"40","author":"Untergasser","year":"2012","journal-title":"Nucl. Acids Res."},{"key":"10.1016\/j.jpba.2017.07.050_bib0090","doi-asserted-by":"crossref","first-page":"1456","DOI":"10.3389\/fmicb.2016.01456","article-title":"Neospora caninum activates p38 MAPK as an evasion mechanism against innate immunity","volume":"7","author":"Mota","year":"2016","journal-title":"Front. Microbiol."},{"key":"10.1016\/j.jpba.2017.07.050_bib0095","doi-asserted-by":"crossref","first-page":"171","DOI":"10.1007\/s12041-017-0750-6","article-title":"A universal rapid, and inexpensive method for genomic DNA isolation from the whole blood of mammals and birds","volume":"96","author":"Al-Shuhaib","year":"2017","journal-title":"J. Genet."},{"key":"10.1016\/j.jpba.2017.07.050_bib0100","doi-asserted-by":"crossref","first-page":"91","DOI":"10.1016\/j.bios.2013.11.072","article-title":"Colorimetric detection of DNA hybridization based on a dual platform of gold nanoparticles and graphene oxide","volume":"55","author":"Thavanathan","year":"2014","journal-title":"Biosens. Bioelectron."},{"key":"10.1016\/j.jpba.2017.07.050_bib0105","doi-asserted-by":"crossref","first-page":"69","DOI":"10.1016\/S0020-7519(99)00170-8","article-title":"Identication of a 200- to 300-fold repetitive 529\u2009bp DNA fragment in Toxoplasma gondii, and its use for diagnostic and quantitative PCR","volume":"30","author":"Homan","year":"2000","journal-title":"Int. J. Parasitol."},{"key":"10.1016\/j.jpba.2017.07.050_bib0110","first-page":"1124","article-title":"Analysis of Toxoplasma gondii repeat region 529\u2009bp (NCBI acc. No. AF146527) as a probe candidate for molecular diagnosis of toxoplasmosis","volume":"14","author":"Pratama","year":"2009","journal-title":"Indones J. Biotechnol."},{"key":"10.1016\/j.jpba.2017.07.050_bib0115","doi-asserted-by":"crossref","first-page":"591","DOI":"10.1128\/JCM.01113-09","article-title":"Comparison of the AF146527 and B1 repeated elements, two real-time PCR targets used for detection of Toxoplasma gondii","volume":"48","author":"Wahab","year":"2010","journal-title":"J. Clin. Microbiol."},{"key":"10.1016\/j.jpba.2017.07.050_bib0120","doi-asserted-by":"crossref","first-page":"37","DOI":"10.1016\/j.parint.2013.08.005","article-title":"Comparison of the RE and B1 gene for detection of Toxoplasma gondii infection in children with cancer","volume":"63","author":"Fallahi","year":"2014","journal-title":"Parasitol. Int."},{"key":"10.1016\/j.jpba.2017.07.050_bib0125","doi-asserted-by":"crossref","DOI":"10.1590\/S1807-59322009000300004","article-title":"Significant performance variation among PCR systems in diagnosing congenital toxoplasmosis in S\u2138o Paulo, Brazil: analysis of 467 amniotic fluid samples","volume":"64","author":"Okay","year":"2009","journal-title":"Clinics"},{"key":"10.1016\/j.jpba.2017.07.050_bib0130","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1186\/1471-2334-13-552","article-title":"Direct genotyping of Toxoplasma gondii from amniotic fluids based on B1 gene polymorphism using minisequencing analysis","volume":"13","author":"Costa","year":"2013","journal-title":"BMC Infect. Dis."},{"key":"10.1016\/j.jpba.2017.07.050_bib0135","doi-asserted-by":"crossref","first-page":"1294","DOI":"10.1128\/JCM.02900-14","article-title":"A 10-year retrospective comparison of two target sequences REP-529 and B1, for Toxoplasma gondii detection by quantitative PCR","volume":"53","author":"Belaz","year":"2015","journal-title":"J. Clin. Microbiol."},{"key":"10.1016\/j.jpba.2017.07.050_bib0140","doi-asserted-by":"crossref","first-page":"637","DOI":"10.1016\/S0020-7519(00)00036-9","article-title":"Molecular typing of Toxoplasma gondii strains by GRA6 gene sequence analysis","volume":"30","author":"Fazaeli","year":"2000","journal-title":"Int. J. Parasitol."},{"key":"10.1016\/j.jpba.2017.07.050_bib0145","doi-asserted-by":"crossref","first-page":"2397","DOI":"10.1091\/mbc.e02-01-0021","article-title":"Biogenesis of nanotubular network in Toxoplasma parasitophorous vacuole induced by parasite proteins","volume":"13","author":"Mercier","year":"2002","journal-title":"Mol. Biol. Cell"},{"issue":"Suppl","key":"10.1016\/j.jpba.2017.07.050_bib0150","doi-asserted-by":"crossref","first-page":"S29","DOI":"10.3347\/kjp.2009.47.S.S29","article-title":"GRA proteins of Toxoplasma gondii: maintenance of host-parasite interactions across the parasitophorous vacuolar membrane","volume":"47","author":"Nam","year":"2009","journal-title":"Korean J. Parasitol."},{"key":"10.1016\/j.jpba.2017.07.050_bib0155","doi-asserted-by":"crossref","first-page":"666","DOI":"10.2478\/s11686-014-0288-1","article-title":"Genetic characterization of Toxoplasma gondii isolates from chickens in India by GRA6 gene sequence analysis","volume":"59","author":"Biradar","year":"2014","journal-title":"Acta Parasitol."},{"key":"10.1016\/j.jpba.2017.07.050_bib0160","doi-asserted-by":"crossref","first-page":"1343","DOI":"10.1128\/CVI.00117-13","article-title":"Toxoplasma gondii recombinant antigens as tools for serodiagnosis of human toxoplasmosis: current status of studies","volume":"20","author":"Holec-Gasior","year":"2013","journal-title":"Clin. Vaccine Immunol."},{"key":"10.1016\/j.jpba.2017.07.050_bib0165","doi-asserted-by":"crossref","first-page":"503","DOI":"10.3347\/kjp.2013.51.5.503","article-title":"A rapid diagnostic test for toxoplasmosis using recombinant antigenic N-terminal half of SAG1 linked with intrinsically unstructured domain of gra2 protein","volume":"51","author":"Song","year":"2013","journal-title":"Korean J. Parasitol."},{"key":"10.1016\/j.jpba.2017.07.050_bib0170","doi-asserted-by":"crossref","first-page":"53","DOI":"10.1016\/j.micres.2015.11.006","article-title":"Immunodiagnosis and molecular validation of Toxoplasma gondii-recombinant dense granular (GRA) 7 protein for the detection of toxoplasmosis in patients with cancer","volume":"183","author":"Arab-Mazar","year":"2016","journal-title":"Microbiol. Res."},{"key":"10.1016\/j.jpba.2017.07.050_bib0175","doi-asserted-by":"crossref","first-page":"120","DOI":"10.1016\/j.actatropica.2016.03.035","article-title":"An alternative nested-PCR assay for the detection of Toxoplasma gondii strains based on GRA7 gene sequences","volume":"159","author":"Costa","year":"2016","journal-title":"Acta Trop."},{"key":"10.1016\/j.jpba.2017.07.050_bib0180","doi-asserted-by":"crossref","first-page":"1853","DOI":"10.1016\/j.msec.2013.01.035","article-title":"A review study of (bio)sensor systems based on conducting polymers","volume":"33","author":"Ates","year":"2013","journal-title":"Mater. Sci. Eng. C Mater. Biol. Appl."},{"key":"10.1016\/j.jpba.2017.07.050_bib0185","doi-asserted-by":"crossref","first-page":"3801","DOI":"10.3390\/s150203801","article-title":"Electrochemical DNA hybrdization sensors based on conducting polymers","volume":"15","author":"Rahman","year":"2015","journal-title":"Sensors (Basel)"},{"key":"10.1016\/j.jpba.2017.07.050_bib0190","doi-asserted-by":"crossref","first-page":"5163","DOI":"10.1093\/nar\/29.24.5163","article-title":"The effect of surface probe densityon DNA hybridization","volume":"29","author":"Peterson","year":"2001","journal-title":"Nucl. Acids Res."},{"key":"10.1016\/j.jpba.2017.07.050_bib0195","doi-asserted-by":"crossref","first-page":"144912","DOI":"10.1063\/1.2787618","article-title":"Computer simulation study of probe-target hybridization in model DNA microarrays: effect of probe surface density and target concentration","volume":"127","author":"Jayaraman","year":"2007","journal-title":"J. Chem. Phys."},{"key":"10.1016\/j.jpba.2017.07.050_bib0200","doi-asserted-by":"crossref","first-page":"41","DOI":"10.1016\/j.ab.2013.09.032","article-title":"Strategies for optimizing DNA hybridization on surfaces","volume":"444","author":"Ravan","year":"2014","journal-title":"Anal. Biochem."},{"key":"10.1016\/j.jpba.2017.07.050_bib0205","doi-asserted-by":"crossref","first-page":"273","DOI":"10.1016\/j.apsusc.2014.06.084","article-title":"Preparation of genosensor for detection of specific DNA sequence of the hepatitis B virus","volume":"314","author":"Honorato Castro","year":"2014","journal-title":"Appl. Surf. Sci."},{"key":"10.1016\/j.jpba.2017.07.050_bib0210","doi-asserted-by":"crossref","first-page":"9051","DOI":"10.3390\/ijms15059051","article-title":"Detection of a specific biomarker for Epstein-Barr virus using a polymer-based genosensor","volume":"15","author":"Balvedi","year":"2014","journal-title":"Int. J. Mol. Sci"},{"key":"10.1016\/j.jpba.2017.07.050_bib0215","doi-asserted-by":"crossref","first-page":"5616","DOI":"10.3390\/s110605616","article-title":"Label-free electrochemical detection of the specific oligonucleotide sequence of dengue virus type 1 on pencil graphite electrodes","volume":"11","author":"Souza","year":"2011","journal-title":"Sensors (Basel)"},{"key":"10.1016\/j.jpba.2017.07.050_bib0220","doi-asserted-by":"crossref","first-page":"92","DOI":"10.1016\/j.snb.2016.04.105","article-title":"Label-free optical biosensor for direct complex DNA detection using Vitis vinifera L","volume":"234","author":"Moreira","year":"2016","journal-title":"Sens. Actuators B"},{"key":"10.1016\/j.jpba.2017.07.050_bib0225","doi-asserted-by":"crossref","first-page":"62","DOI":"10.1016\/j.bios.2017.04.024","article-title":"Proximity hybridization triggered hemin\/G-quadruplex formation for construction a label-free and signal-on electrochemical DNA sensor","volume":"96","author":"Gao","year":"2017","journal-title":"Biosens. Bioelectron."},{"key":"10.1016\/j.jpba.2017.07.050_bib0230","doi-asserted-by":"crossref","first-page":"6215","DOI":"10.1039\/C7CC03239K","article-title":"A label-free ratiometric electrochemical DNA sensor for monitoring intracellular redox homeostasis","volume":"53","author":"Liu","year":"2017","journal-title":"Chem. Commun."},{"key":"10.1016\/j.jpba.2017.07.050_bib0235","doi-asserted-by":"crossref","first-page":"199","DOI":"10.1016\/j.msec.2016.04.030","article-title":"In vitro cytotoxicity and phototoxicity of surface-modified gold nanoparticles associated with neutral red as a potential drug delivery system in phototherapy","volume":"65","author":"Verissimo","year":"2016","journal-title":"Mater. Sci. Eng. C Mater. Biol. Appl."}],"container-title":["Journal of Pharmaceutical and Biomedical Analysis"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0731708517306106?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0731708517306106?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2023,8,24]],"date-time":"2023-08-24T22:46:48Z","timestamp":1692917208000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0731708517306106"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,10]]},"references-count":47,"alternative-id":["S0731708517306106"],"URL":"https:\/\/doi.org\/10.1016\/j.jpba.2017.07.050","relation":{},"ISSN":["0731-7085"],"issn-type":[{"value":"0731-7085","type":"print"}],"subject":[],"published":{"date-parts":[[2017,10]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Development of direct assays for Toxoplasma gondii and its use in genomic DNA sample","name":"articletitle","label":"Article Title"},{"value":"Journal of Pharmaceutical and Biomedical Analysis","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.jpba.2017.07.050","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2017 Elsevier B.V. All rights reserved.","name":"copyright","label":"Copyright"}]}}