{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,7]],"date-time":"2026-09-07T15:17:45Z","timestamp":1788794265759,"version":"build-2803163510"},"reference-count":30,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2019,2,1]],"date-time":"2019-02-01T00:00:00Z","timestamp":1548979200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2019,2,1]],"date-time":"2019-02-01T00:00:00Z","timestamp":1548979200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2019,2,1]],"date-time":"2019-02-01T00:00:00Z","timestamp":1548979200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-017"},{"start":{"date-parts":[[2019,2,1]],"date-time":"2019-02-01T00:00:00Z","timestamp":1548979200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2019,2,1]],"date-time":"2019-02-01T00:00:00Z","timestamp":1548979200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-012"},{"start":{"date-parts":[[2019,2,1]],"date-time":"2019-02-01T00:00:00Z","timestamp":1548979200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2019,2,1]],"date-time":"2019-02-01T00:00:00Z","timestamp":1548979200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-004"}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Journal of Pharmaceutical and Biomedical Analysis"],"published-print":{"date-parts":[[2019,2]]},"DOI":"10.1016\/j.jpba.2018.10.039","type":"journal-article","created":{"date-parts":[[2018,10,25]],"date-time":"2018-10-25T18:38:40Z","timestamp":1540492720000},"page":"317-325","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":7,"special_numbering":"C","title":["Preliminary pharmacokinetic study of the anticancer 6BIO in mice using an UHPLC-MS\/MS approach"],"prefix":"10.1016","volume":"164","author":[{"given":"Job","family":"Tchoumtchoua","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria","family":"Halabalaki","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0107-9991","authenticated-orcid":false,"given":"Evangelos","family":"Gikas","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6843-5575","authenticated-orcid":false,"given":"Anthony","family":"Tsarbopoulos","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nikoletta","family":"Fotaki","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lucy","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sangkil","family":"Nam","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Richard","family":"Jove","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Leandros A.","family":"Skaltsounis","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"issue":"5","key":"10.1016\/j.jpba.2018.10.039_bib0005","doi-asserted-by":"crossref","first-page":"977","DOI":"10.1021\/np5008763","article-title":"NMR-Based Metabolomic Study on Isatis tinctoria: Comparison of Different Accessions, Harvesting Dates, and the Effect of Repeated Harvesting","volume":"78","author":"Guldbrandsen","year":"2015","journal-title":"J. Nat. Prod."},{"issue":"7","key":"10.1016\/j.jpba.2018.10.039_bib0010","doi-asserted-by":"crossref","first-page":"1239","DOI":"10.1002\/rcm.2954","article-title":"Determination of indican, isatin, indirubin and indigotin in Isatis indigotica by liquid chromatography\/electrospray ionization tandem mass spectrometry","volume":"21","author":"Zou","year":"2007","journal-title":"Rapid Commun. Mass Spectrom."},{"issue":"6","key":"10.1016\/j.jpba.2018.10.039_bib0015","doi-asserted-by":"crossref","first-page":"1695","DOI":"10.1007\/s00216-014-8423-2","article-title":"Identification of natural indigo in historical textiles by GC\u2013MS","volume":"407","author":"Degani","year":"2015","journal-title":"Anal. Bioanal. Chem."},{"issue":"5","key":"10.1016\/j.jpba.2018.10.039_bib0020","doi-asserted-by":"crossref","first-page":"1104","DOI":"10.1021\/bp025540+","article-title":"\u03b2-glucosidase-catalyzed hydrolysis of indican from leaves of Polygonum tinctorium","volume":"18","author":"Maugard","year":"2002","journal-title":"Biotechnol. Prog."},{"key":"10.1016\/j.jpba.2018.10.039_bib0025","article-title":"Rapid Identification and Verification of Indirubin-Containing Medicinal Plants","volume":"2015","author":"Hu","year":"2015","journal-title":"Evid. Complement. Alternat. Med."},{"key":"10.1016\/j.jpba.2018.10.039_bib0030","doi-asserted-by":"crossref","first-page":"122","DOI":"10.1016\/j.ejps.2015.08.015","article-title":"5-Nitro-5\u2032-hydroxy-indirubin-3\u2032-oxime (AGM130), an indirubin-3\u2032-oxime derivative, inhibits tumor growth by inducing apoptosis against non-small cell lung cancer in vitro and in vivo","volume":"79","author":"Ahn","year":"2015","journal-title":"Eur. J. Pharm. Sci."},{"issue":"9","key":"10.1016\/j.jpba.2018.10.039_bib0035","doi-asserted-by":"crossref","first-page":"1244","DOI":"10.1016\/j.chroma.2011.01.004","article-title":"High performance liquid chromatography of slightly soluble brominated indigoids from Tyrian purple","volume":"1218","author":"Nowik","year":"2011","journal-title":"J. Chromatogr. A"},{"issue":"1","key":"10.1016\/j.jpba.2018.10.039_bib0040","doi-asserted-by":"crossref","first-page":"7","DOI":"10.1016\/j.dyepig.2009.03.019","article-title":"Brominated precursors of Tyrian purple (C.I. Natural Violet 1) from Plicopurpura pansa, Plicopurpura columellaris and Plicopurpura patula","volume":"83","author":"L\u00f3pez Ch\u00e1vez","year":"2009","journal-title":"Dye. Pigment."},{"issue":"9","key":"10.1016\/j.jpba.2018.10.039_bib0045","doi-asserted-by":"crossref","first-page":"736","DOI":"10.3390\/60900736","article-title":"Tyrian Purple: 6,6\u2019-Dibromoindigo and Related Compounds","volume":"6","author":"Cooksey","year":"2001","journal-title":"Molecules."},{"key":"10.1016\/j.jpba.2018.10.039_bib0050","first-page":"6","article-title":"Chemical constituents of traditional Chinese medicine Qing Dai","volume":"15","author":"Chen","year":"1984","journal-title":"Chinese Trad Herb Drugs."},{"key":"10.1016\/j.jpba.2018.10.039_bib0055","first-page":"6","article-title":"Studies on the active principles of indigofera tinctoria in the treatment of CML","volume":"9","author":"Wu","year":"1979","journal-title":"Comm Chinese Herb Med."},{"issue":"1","key":"10.1016\/j.jpba.2018.10.039_bib0060","doi-asserted-by":"crossref","first-page":"60","DOI":"10.1038\/9035","article-title":"Indirubin, the active constituent of a Chinese antileukaemia medicine, inhibits cyclin-dependent kinases","volume":"1","author":"Hoessel","year":"1999","journal-title":"Nat. Cell Biol."},{"issue":"8","key":"10.1016\/j.jpba.2018.10.039_bib0065","doi-asserted-by":"crossref","first-page":"5237","DOI":"10.3390\/md13085237","article-title":"Are the traditional medical uses of Muricidae molluscs substantiated by their pharmacological properties and bioactive compounds?","volume":"13","author":"Benkendorff","year":"2015","journal-title":"Mar. Drugs"},{"issue":"12","key":"10.1016\/j.jpba.2018.10.039_bib0070","doi-asserted-by":"crossref","first-page":"1255","DOI":"10.1016\/j.chembiol.2003.11.010","article-title":"GSK-3-Selective Inhibitors Derived from Tyrian Purple Indirubins","volume":"10","author":"Meijer","year":"2003","journal-title":"Chem. Biol."},{"issue":"5","key":"10.1016\/j.jpba.2018.10.039_bib0075","doi-asserted-by":"crossref","first-page":"583","DOI":"10.1517\/13543776.2015.1019865","article-title":"Indirubin derivatives: a patent review (2010 \u2013 present)","volume":"25","author":"Gaboriaud-Kolar","year":"2015","journal-title":"Expert Opin. Ther. Pat."},{"issue":"9","key":"10.1016\/j.jpba.2018.10.039_bib0080","doi-asserted-by":"crossref","first-page":"1213","DOI":"10.2174\/1871520614666140825112924","article-title":"New Perspective on the Dual Functions of Indirubins in Cancer Therapy and Neuroprotection","volume":"14","author":"Wang","year":"2014","journal-title":"Anti-Cancer Agents in Medicinal Chemistry- Anti-Cancer Agents)."},{"issue":"14","key":"10.1016\/j.jpba.2018.10.039_bib0085","doi-asserted-by":"crossref","first-page":"1515","DOI":"10.1055\/s-0032-1315261","article-title":"From tyrian purple to kinase modulators: Naturally halogenated indirubins and synthetic analogues","volume":"78","author":"Vougogiannopoulou","year":"2012","journal-title":"Planta Med."},{"issue":"9","key":"10.1016\/j.jpba.2018.10.039_bib0090","doi-asserted-by":"crossref","first-page":"3715","DOI":"10.1021\/jm401740w","article-title":"Indirubin Core Structure of Glycogen Synthase Kinase-3 Inhibitors as Novel Chemotype for Intervention with 5-Lipoxygenase","volume":"57","author":"Pergola","year":"2014","journal-title":"J. Med. Chem."},{"issue":"1","key":"10.1016\/j.jpba.2018.10.039_bib0095","doi-asserted-by":"crossref","first-page":"22","DOI":"10.1021\/ml300207a","article-title":"Novel Inverse Binding Mode of Indirubin Derivatives Yields Improved Selectivity for DYRK Kinases","volume":"4","author":"Myrianthopoulos","year":"2013","journal-title":"ACS Med. Chem. Lett."},{"issue":"3","key":"10.1016\/j.jpba.2018.10.039_bib0100","doi-asserted-by":"crossref","first-page":"703","DOI":"10.1002\/prot.22890","article-title":"Kinetics, in silico docking, molecular dynamics, and MM-GBSA binding studies on prototype indirubins, KT5720, and staurosporine as phosphorylase kinase ATP-binding site inhibitors: The role of water molecules examined","volume":"79","author":"Hayes","year":"2011","journal-title":"Proteins Struct. Funct. Bioinform."},{"issue":"12","key":"10.1016\/j.jpba.2018.10.039_bib0105","doi-asserted-by":"crossref","first-page":"1289","DOI":"10.1016\/j.ijpara.2009.04.005","article-title":"6-Br-5methylindirubin-3\u2032oxime (5-Me-6-BIO) targeting the leishmanial glycogen synthase kinase-3 (GSK-3) short form affects cell-cycle progression and induces apoptosis-like death: Exploitation of GSK-3 for treating leishmaniasis","volume":"39","author":"Xingi","year":"2009","journal-title":"Int. J. Parasitol."},{"issue":"12","key":"10.1016\/j.jpba.2018.10.039_bib0110","doi-asserted-by":"crossref","first-page":"2199","DOI":"10.1021\/np9003905","article-title":"Synthesis and Antiproliferative Activity of 7-Azaindirubin-3\u2032-oxime, a 7-Aza Isostere of the Natural Indirubin Pharmacophore","volume":"72","author":"Kritsanida","year":"2009","journal-title":"J. Nat. Prod."},{"issue":"4","key":"10.1016\/j.jpba.2018.10.039_bib0115","doi-asserted-by":"crossref","first-page":"935","DOI":"10.1021\/jm031016d","article-title":"Structural Basis for the Synthesis of Indirubins as Potent and Selective Inhibitors of Glycogen Synthase Kinase-3 and Cyclin-Dependent Kinases","volume":"47","author":"Polychronopoulos","year":"2004","journal-title":"J. Med. Chem."},{"issue":"1","key":"10.1016\/j.jpba.2018.10.039_bib0120","doi-asserted-by":"crossref","first-page":"55","DOI":"10.1038\/nm979","article-title":"Maintenance of pluripotency in human and mouse embryonic stem cells through activation of Wnt signaling by a pharmacological GSK-3-specific inhibitor","volume":"10","author":"Sato","year":"2004","journal-title":"Nat. Med."},{"issue":"11","key":"10.1016\/j.jpba.2018.10.039_bib0125","doi-asserted-by":"crossref","first-page":"3972","DOI":"10.1158\/0008-5472.CAN-10-3852","article-title":"6-Bromoindirubin-3\u2032-oxime inhibits JAK\/STAT3 signaling and induces apoptosis of human melanoma cells","volume":"71","author":"Liu","year":"2011","journal-title":"Cancer Res."},{"issue":"19","key":"10.1016\/j.jpba.2018.10.039_bib0130","doi-asserted-by":"crossref","first-page":"6004","DOI":"10.1158\/0008-5472.CAN-12-4358","article-title":"Indirubin derivative 6BIO suppresses metastasis","volume":"73","author":"Braig","year":"2013","journal-title":"Cancer Res."},{"issue":"2","key":"10.1016\/j.jpba.2018.10.039_bib0135","doi-asserted-by":"crossref","first-page":"157","DOI":"10.1016\/j.bcp.2014.07.009","article-title":"The pleiotropic profile of the indirubin derivative 6BIO overcomes TRAIL resistance in cancer","volume":"91","author":"Braig","year":"2014","journal-title":"Biochem. Pharmacol."},{"issue":"5","key":"10.1016\/j.jpba.2018.10.039_bib0140","doi-asserted-by":"crossref","first-page":"561","DOI":"10.1002\/jms.3167","article-title":"Structure-oriented UHPLC-LTQ Orbitrap-based approach as a dereplication strategy for the identification of isoflavonoids from Amphimas pterocarpoides crude extract","volume":"48","author":"Tchoumtchoua","year":"2013","journal-title":"J. Mass Spectrom."},{"issue":"4","key":"10.1016\/j.jpba.2018.10.039_bib0145","doi-asserted-by":"crossref","first-page":"277","DOI":"10.1016\/j.phymed.2008.01.004","article-title":"Determination and pharmacokinetic study of indirubin in rat plasma by high-performance liquid chromatography","volume":"15","author":"Deng","year":"2008","journal-title":"Phytomedicine."},{"issue":"4","key":"10.1016\/j.jpba.2018.10.039_bib0150","doi-asserted-by":"crossref","first-page":"1724","DOI":"10.3892\/ijo.2014.2548","article-title":"Evaluation of meisoindigo, an indirubin derivative: In vitro antileukemic activity and in vivo pharmacokinetics","volume":"45","author":"Huang","year":"2014","journal-title":"Int. J. Oncol."}],"container-title":["Journal of Pharmaceutical and Biomedical Analysis"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0731708518319289?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0731708518319289?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2024,6,1]],"date-time":"2024-06-01T19:33:19Z","timestamp":1717270399000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0731708518319289"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,2]]},"references-count":30,"alternative-id":["S0731708518319289"],"URL":"https:\/\/doi.org\/10.1016\/j.jpba.2018.10.039","relation":{},"ISSN":["0731-7085"],"issn-type":[{"value":"0731-7085","type":"print"}],"subject":[],"published":{"date-parts":[[2019,2]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Preliminary pharmacokinetic study of the anticancer 6BIO in mice using an UHPLC-MS\/MS approach","name":"articletitle","label":"Article Title"},{"value":"Journal of Pharmaceutical and Biomedical Analysis","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.jpba.2018.10.039","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2018 Elsevier B.V. All rights reserved.","name":"copyright","label":"Copyright"}]}}