{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,3]],"date-time":"2026-03-03T01:29:08Z","timestamp":1772501348794,"version":"3.50.1"},"reference-count":31,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2019,5,1]],"date-time":"2019-05-01T00:00:00Z","timestamp":1556668800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Journal of Pharmaceutical and Biomedical Analysis"],"published-print":{"date-parts":[[2019,5]]},"DOI":"10.1016\/j.jpba.2019.02.025","type":"journal-article","created":{"date-parts":[[2019,2,19]],"date-time":"2019-02-19T16:52:34Z","timestamp":1550595154000},"page":"201-208","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":33,"special_numbering":"C","title":["Development and validation of an HPLC-UV method for simultaneous determination of sildenafil and tramadol in biological fluids: Application to drug-drug interaction study"],"prefix":"10.1016","volume":"168","author":[{"given":"Hosam Eldin","family":"Dahshan","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mohamed A.","family":"Helal","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Samia M.","family":"Mostafa","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mohamed Saleh","family":"Elgawish","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1016\/j.jpba.2019.02.025_sbref0005a","doi-asserted-by":"crossref","first-page":"576","DOI":"10.1111\/j.1743-6109.2011.02585.x","volume":"9","author":"Shindel","year":"2012","journal-title":"J. Sex Med."},{"key":"10.1016\/j.jpba.2019.02.025_sbref0005b","doi-asserted-by":"crossref","first-page":"257","DOI":"10.1046\/j.1464-410X.1996.10220.x","volume":"78","author":"Boolell","year":"1996","journal-title":"Br. J. Urol."},{"key":"10.1016\/j.jpba.2019.02.025_sbref0005c","doi-asserted-by":"crossref","first-page":"54","DOI":"10.1016\/S0022-5347(17)34871-1","volume":"151","author":"Feldman","year":"1994","journal-title":"J. Urol."},{"key":"10.1016\/j.jpba.2019.02.025_bib0010","doi-asserted-by":"crossref","first-page":"51","DOI":"10.3923\/tmr.2015.51.62","volume":"10","author":"Oka","year":"2015","journal-title":"Trend Med Res"},{"key":"10.1016\/j.jpba.2019.02.025_bib0015","series-title":"Foye\u2019s Principles of Medicinal Chemistry","author":"Foye","year":"2008"},{"key":"10.1016\/j.jpba.2019.02.025_bib0020","series-title":"Clarke\u2019s Analysis of Drugs and Poison","author":"Anthony","year":"2011"},{"key":"10.1016\/j.jpba.2019.02.025_bib0025","first-page":"1","volume":"1010","author":"Al-Hroub","year":"2016","journal-title":"B: Anal. Technol. Biomed. Life Sci."},{"key":"10.1016\/j.jpba.2019.02.025_bib0030","doi-asserted-by":"crossref","first-page":"952","DOI":"10.1016\/j.jpba.2005.08.022","volume":"40","author":"Guermouche","year":"2006","journal-title":"J. Pharm. Biomed. Anal."},{"key":"10.1016\/j.jpba.2019.02.025_bib0035","doi-asserted-by":"crossref","first-page":"255","DOI":"10.1016\/S1570-0232(03)00223-X","volume":"791","author":"Sheu","year":"2003","journal-title":"J. Chromatogr. B."},{"key":"10.1016\/j.jpba.2019.02.025_bib0040","doi-asserted-by":"crossref","first-page":"1559","DOI":"10.1002\/bmc.3459","volume":"29","author":"Stracha","year":"2015","journal-title":"Biomed. Chromagr"},{"key":"10.1016\/j.jpba.2019.02.025_bib0045","doi-asserted-by":"crossref","first-page":"88","DOI":"10.1093\/jat\/27.2.88","volume":"27","author":"Tracqui","year":"2003","journal-title":"J. Anal. Toxicol."},{"key":"10.1016\/j.jpba.2019.02.025_bib0050","doi-asserted-by":"crossref","first-page":"252","DOI":"10.1007\/s004140000178","volume":"114","author":"Weinmann","year":"2001","journal-title":"Int. J. Legal Med."},{"key":"10.1016\/j.jpba.2019.02.025_bib0055","doi-asserted-by":"crossref","first-page":"21","DOI":"10.1016\/j.jpba.2016.06.022","volume":"129","author":"Rashid","year":"2016","journal-title":"J. Pharm. Biomed. Anal."},{"key":"10.1016\/j.jpba.2019.02.025_bib0060","doi-asserted-by":"crossref","first-page":"529","DOI":"10.1016\/j.jpba.2012.04.030","volume":"70","author":"Unceta","year":"2012","journal-title":"J. Pharm. Biomed. Anal."},{"key":"10.1016\/j.jpba.2019.02.025_bib0065","doi-asserted-by":"crossref","first-page":"325","DOI":"10.1016\/S0378-4347(01)00237-7","volume":"759","author":"Gan","year":"2001","journal-title":"J. Chromatogr. B, Biomed. Appl."},{"key":"10.1016\/j.jpba.2019.02.025_bib0070","first-page":"123","volume":"772","author":"Gan","year":"2002","journal-title":"B: Anal. Technol. Biomed. Life Sci."},{"key":"10.1016\/j.jpba.2019.02.025_bib0075","doi-asserted-by":"crossref","first-page":"194","DOI":"10.1016\/j.jpba.2010.03.016","volume":"53","author":"Saccomanni","year":"2010","journal-title":"J. Pharm. Biomed. Anal."},{"key":"10.1016\/j.jpba.2019.02.025_bib0080","doi-asserted-by":"crossref","first-page":"1104","DOI":"10.1016\/j.talanta.2008.01.006","volume":"75","author":"Vlase","year":"2008","journal-title":"Talanta"},{"key":"10.1016\/j.jpba.2019.02.025_bib0085","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1186\/s40780-015-0033-4","volume":"2","author":"Hironari","year":"2016","journal-title":"J. Pharm. Health Care Sci."},{"key":"10.1016\/j.jpba.2019.02.025_bib0090","doi-asserted-by":"crossref","first-page":"1243","DOI":"10.1002\/dta.2157","volume":"9","author":"Liu","year":"2017","journal-title":"Drug Test. Anal."},{"key":"10.1016\/j.jpba.2019.02.025_bib0095","first-page":"2682","volume":"11","author":"Farag","year":"2003","journal-title":"Int. J. Comput. Technol."},{"key":"10.1016\/j.jpba.2019.02.025_bib0100","doi-asserted-by":"crossref","first-page":"104","DOI":"10.1038\/sj.ijir.3901420","volume":"18","author":"Dresser","year":"2006","journal-title":"Int. J. Impot. Res."},{"key":"10.1016\/j.jpba.2019.02.025_bib0105","doi-asserted-by":"crossref","first-page":"306","DOI":"10.1016\/j.cmpb.2010.01.007","volume":"99","author":"Zhang","year":"2010","journal-title":"Comput. Methods Programs Biomed."},{"key":"10.1016\/j.jpba.2019.02.025_bib0110","doi-asserted-by":"crossref","first-page":"1409","DOI":"10.1021\/tx400253k","volume":"16","author":"Elgawish","year":"2013","journal-title":"Chem. Res. Toxicol."},{"key":"10.1016\/j.jpba.2019.02.025_bib0115","unstructured":"U.S. Department of Health and Human Services Food and Drug Administration, Center for Drug Evaluation and Research (CDER), and Center for Veterinary Medicine (CVM), Guidance for Industry, Bioanalytical Method Validation, http:\/\/www.fda.gov\/downloads\/Drugs\/Guidances\/ucm070107.pdf (accessed May, 2001)."},{"key":"10.1016\/j.jpba.2019.02.025_bib0120","doi-asserted-by":"crossref","first-page":"297","DOI":"10.1080\/004982599238687","volume":"29","author":"Walker","year":"1999","journal-title":"Xenobiotica"},{"key":"10.1016\/j.jpba.2019.02.025_bib0125","doi-asserted-by":"crossref","first-page":"389","DOI":"10.1146\/annurev.pharmtox.38.1.389","volume":"38","author":"Thummel","year":"1998","journal-title":"Annu. Rev. Pharmacol. Toxicol."},{"key":"10.1016\/j.jpba.2019.02.025_bib0130","doi-asserted-by":"crossref","first-page":"4137","DOI":"10.1021\/bi9715627","volume":"37","author":"Korzekwa","year":"1998","journal-title":"Biochemistry"},{"key":"10.1016\/j.jpba.2019.02.025_bib0135","doi-asserted-by":"crossref","first-page":"51","DOI":"10.3923\/tmr.2015.51.62","volume":"10","author":"Oka","year":"2015","journal-title":"Trends in Med Res."},{"key":"10.1016\/j.jpba.2019.02.025_bib0140","doi-asserted-by":"crossref","first-page":"105","DOI":"10.7324\/JAPS.2015.50817","volume":"5","author":"Nna","year":"2015","journal-title":"J. Appl. Pharma Sci."},{"key":"10.1016\/j.jpba.2019.02.025_bib0145","first-page":"162","volume":"4","author":"Aldalou","year":"2014","journal-title":"J. Sci."}],"container-title":["Journal of Pharmaceutical and Biomedical Analysis"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0731708519301384?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0731708519301384?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2019,3,29]],"date-time":"2019-03-29T23:09:49Z","timestamp":1553900989000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0731708519301384"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,5]]},"references-count":31,"alternative-id":["S0731708519301384"],"URL":"https:\/\/doi.org\/10.1016\/j.jpba.2019.02.025","relation":{},"ISSN":["0731-7085"],"issn-type":[{"value":"0731-7085","type":"print"}],"subject":[],"published":{"date-parts":[[2019,5]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Development and validation of an HPLC-UV method for simultaneous determination of sildenafil and tramadol in biological fluids: Application to drug-drug interaction study","name":"articletitle","label":"Article Title"},{"value":"Journal of Pharmaceutical and Biomedical Analysis","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.jpba.2019.02.025","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2019 Elsevier B.V. All rights reserved.","name":"copyright","label":"Copyright"}]}}