{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,15]],"date-time":"2026-05-15T15:19:39Z","timestamp":1778858379864,"version":"3.51.4"},"reference-count":39,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2019,5,1]],"date-time":"2019-05-01T00:00:00Z","timestamp":1556668800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Journal of Pharmaceutical and Biomedical Analysis"],"published-print":{"date-parts":[[2019,5]]},"DOI":"10.1016\/j.jpba.2019.02.029","type":"journal-article","created":{"date-parts":[[2019,2,22]],"date-time":"2019-02-22T03:30:58Z","timestamp":1550806258000},"page":"30-40","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":14,"special_numbering":"C","title":["Quantitative determination of bioactive proteins in diphtheria tetanus acellular pertussis (DTaP) vaccine by liquid chromatography tandem mass spectrometry"],"prefix":"10.1016","volume":"169","author":[{"given":"Zhen","family":"Long","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chen","family":"Wei","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhaoqi","family":"Zhan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiao","family":"Ma","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiuling","family":"Li","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yueqi","family":"Li","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jinting","family":"Yao","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Feng","family":"Ji","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Changkun","family":"Li","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Taohong","family":"Huang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"issue":"3 Pt 2","key":"10.1016\/j.jpba.2019.02.029_bib0005","doi-asserted-by":"crossref","first-page":"548","DOI":"10.1542\/peds.96.3.548","article-title":"Comparison of 13 acellular pertussis vaccines: overview and serologic response","volume":"96","author":"Edwards","year":"1995","journal-title":"Pediatrics"},{"issue":"6","key":"10.1016\/j.jpba.2019.02.029_bib0010","doi-asserted-by":"crossref","first-page":"341","DOI":"10.1056\/NEJM199602083340601","article-title":"A controlled trial of two acellular vaccines and one whole-cell vaccine against pertussis","volume":"334","author":"Pizza","year":"1996","journal-title":"N. Engl. J. Med."},{"issue":"7","key":"10.1016\/j.jpba.2019.02.029_bib0015","doi-asserted-by":"crossref","first-page":"978","DOI":"10.1093\/infdis\/jiu001","article-title":"Changing pertussis epidemiology: everything old is new again","volume":"209","author":"Clark","year":"2014","journal-title":"J. Infect. Dis."},{"key":"10.1016\/j.jpba.2019.02.029_bib0020","unstructured":"http:\/\/www.who.int\/en\/news-room\/fact-sheets\/detail\/immunization-coverage."},{"issue":"5","key":"10.1016\/j.jpba.2019.02.029_bib0025","first-page":"481","article-title":"Adverse events following immunization in China in 2015","author":"Ye","year":"2017","journal-title":"Chin. J. Vaccine Immun."},{"issue":"1","key":"10.1016\/j.jpba.2019.02.029_bib0030","doi-asserted-by":"crossref","first-page":"16","DOI":"10.1016\/j.ab.2007.10.001","article-title":"Characterization of pertussis toxin by LC\u2013MS\/MS","volume":"374","author":"Tummala","year":"2008","journal-title":"Anal. Biochem."},{"issue":"29","key":"10.1016\/j.jpba.2019.02.029_bib0035","doi-asserted-by":"crossref","first-page":"17759","DOI":"10.1016\/S0021-9258(18)38228-0","article-title":"The subunit S1 is important for pertussis toxin secretion","volume":"265","author":"Pizza","year":"1990","journal-title":"J. Biol. Chem."},{"issue":"3","key":"10.1016\/j.jpba.2019.02.029_bib0040","doi-asserted-by":"crossref","first-page":"235","DOI":"10.1111\/j.1574-695X.1999.tb01244.x","article-title":"Identification of immunodominant epitopes in the filamentous hemagglutinin of Bordetella pertussis","volume":"23","author":"Piatti","year":"1999","journal-title":"FEMS Immunol. Med. Mic."},{"issue":"11","key":"10.1016\/j.jpba.2019.02.029_bib0045","doi-asserted-by":"crossref","first-page":"1570","DOI":"10.1016\/j.vaccine.2003.09.040","article-title":"Rapid purification of pertussis toxin (PT) and filamentous hemagglutinin (FHA) by cation-exchange chromatography","volume":"22","author":"\u00d6zcengiz","year":"2004","journal-title":"Vaccine"},{"issue":"10","key":"10.1016\/j.jpba.2019.02.029_bib0050","doi-asserted-by":"crossref","first-page":"3554","DOI":"10.1073\/pnas.86.10.3554","article-title":"Molecular cloning and characterization of protective outer membrane protein P.69 from Bordetella pertussis","volume":"86","author":"Charles","year":"1989","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"issue":"34","key":"10.1016\/j.jpba.2019.02.029_bib0055","doi-asserted-by":"crossref","first-page":"4032","DOI":"10.1016\/j.vaccine.2016.06.029","article-title":"Purification design and practice for pertactin, the third component of acellular pertussis vaccine, from Bordetella pertussis","volume":"34","author":"Li","year":"2016","journal-title":"Vaccine"},{"key":"10.1016\/j.jpba.2019.02.029_bib0060","doi-asserted-by":"crossref","first-page":"15","DOI":"10.1016\/j.ab.2017.10.025","article-title":"An ELISA method to estimate the mono ADP-ribosyltransferase activities: e.g in pertussis toxin and vaccines","volume":"540\u2013541","author":"Asokanathan","year":"2018","journal-title":"Anal. Biochem."},{"issue":"Supplement C","key":"10.1016\/j.jpba.2019.02.029_bib0065","doi-asserted-by":"crossref","first-page":"23","DOI":"10.1016\/j.jchromb.2016.04.001","article-title":"Challenges and opportunities of using liquid chromatography and mass spectrometry methods to develop complex vaccine antigens as pharmaceutical dosage forms","volume":"1032","author":"Hickey","year":"2016","journal-title":"J. Chromatogr. B"},{"issue":"4","key":"10.1016\/j.jpba.2019.02.029_bib0070","doi-asserted-by":"crossref","first-page":"317","DOI":"10.1021\/pr025517j","article-title":"Quantitative profiling of proteins in complex mixtures using liquid chromatography and mass spectrometry","volume":"1","author":"Chelius","year":"2002","journal-title":"J. Proteome Res."},{"key":"10.1016\/j.jpba.2019.02.029_bib0075","doi-asserted-by":"crossref","first-page":"161","DOI":"10.1016\/j.jchromb.2013.04.030","article-title":"Bioanalytical LC\u2013MS\/MS of protein-based biopharmaceuticals","volume":"929","author":"van den Broek","year":"2013","journal-title":"J. Chromatogr. B"},{"issue":"32","key":"10.1016\/j.jpba.2019.02.029_bib0080","doi-asserted-by":"crossref","first-page":"4762","DOI":"10.1016\/j.vaccine.2012.05.036","article-title":"Simultaneous quantification of the viral antigens hemagglutinin and neuraminidase in influenza vaccines by LC\u2013MSE","volume":"30","author":"Creskey","year":"2012","journal-title":"Vaccine"},{"key":"10.1016\/j.jpba.2019.02.029_bib0085","doi-asserted-by":"crossref","first-page":"253","DOI":"10.1016\/j.jpba.2018.04.006","article-title":"A fast and robust hydrophilic interaction liquid chromatography tandem mass spectrometry method for determining methylpentose, hexose, hexosamine and hexonic acid in pneumococcal polysaccharide vaccine hydrolysates","volume":"155","author":"Long","year":"2018","journal-title":"J. Pharm. Biomed. Anal."},{"key":"10.1016\/j.jpba.2019.02.029_bib0090","unstructured":"https:\/\/www.nibsc.org\/products\/brm_product_catalogue\/detail_page.aspx?catid=15\/126."},{"issue":"16","key":"10.1016\/j.jpba.2019.02.029_bib0095","first-page":"30","article-title":"International collaborative calibration for the 2nd generation WHO reference standard of pertussis toxin","volume":"27","author":"Wei","year":"2018","journal-title":"Chin. J. N. Drug."},{"issue":"7","key":"10.1016\/j.jpba.2019.02.029_bib0100","doi-asserted-by":"crossref","first-page":"938","DOI":"10.1016\/j.chroma.2010.12.067","article-title":"First screening method for the simultaneous detection of seven allergens by liquid chromatography mass spectrometry","volume":"1218","author":"Heick","year":"2011","journal-title":"J. Chromatogr. A"},{"key":"10.1016\/j.jpba.2019.02.029_bib0105","series-title":"Methods in \u2009Molecular Biology","doi-asserted-by":"crossref","DOI":"10.1007\/978-1-4939-6700-1","author":"Gao","year":"2017"},{"issue":"4","key":"10.1016\/j.jpba.2019.02.029_bib0110","doi-asserted-by":"crossref","first-page":"351","DOI":"10.1016\/j.ymeth.2011.05.005","article-title":"The importance of the digest: proteolysis and absolute quantification in proteomics","volume":"54","author":"Brownridge","year":"2011","journal-title":"Methods"},{"issue":"21","key":"10.1016\/j.jpba.2019.02.029_bib0115","doi-asserted-by":"crossref","first-page":"6023","DOI":"10.1021\/ac0346196","article-title":"Enzyme-friendly, mass spectrometry-compatible surfactant for in-solution enzymatic digestion of proteins","volume":"75","author":"Ying-Qing","year":"2003","journal-title":"Anal. Chem."},{"key":"10.1016\/j.jpba.2019.02.029_bib0120","volume":"vol. III","author":"Chinese Pharmacopoeia Commission","year":"2015"},{"key":"10.1016\/j.jpba.2019.02.029_bib0125","series-title":"Reductive Cleavage of Cystine Disulfides with Tributylphosphine, Methods in Enzymology","first-page":"111","author":"Ruegg","year":"1977"},{"issue":"26","key":"10.1016\/j.jpba.2019.02.029_bib0130","doi-asserted-by":"crossref","first-page":"12485","DOI":"10.1073\/pnas.92.26.12485","article-title":"Peptide synthesis using unprotected peptides through orthogonal coupling methods","volume":"92","author":"T. J P","year":"1995","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"issue":"1","key":"10.1016\/j.jpba.2019.02.029_bib0135","doi-asserted-by":"crossref","first-page":"235","DOI":"10.1016\/j.chroma.2007.01.045","article-title":"Trends in sample preparation for classical and second generation proteomics","volume":"1153","author":"Ca\u00f1as","year":"2007","journal-title":"J. Chromatogr. A"},{"issue":"12","key":"10.1016\/j.jpba.2019.02.029_bib0140","doi-asserted-by":"crossref","first-page":"735","DOI":"10.1016\/j.mpmed.2017.09.015","article-title":"Pertussis","volume":"45","author":"Calvert","year":"2017","journal-title":"Medicine"},{"issue":"Supplement C","key":"10.1016\/j.jpba.2019.02.029_bib0145","doi-asserted-by":"crossref","first-page":"16","DOI":"10.1016\/j.chroma.2015.11.077","article-title":"Recent advances and trends in the liquid-chromatography\u2013mass spectrometry analysis of flavonoids","volume":"1430","author":"de Villiers","year":"2016","journal-title":"J. Chromatogr. A"},{"key":"10.1016\/j.jpba.2019.02.029_bib0150","doi-asserted-by":"crossref","first-page":"99","DOI":"10.1016\/j.talanta.2012.01.054","article-title":"Evaluation of new mixed-mode UHPLC stationary phases and the importance of stationary phase choice when using low ionic-strength mobile phase additives","volume":"93","author":"Lucie","year":"2012","journal-title":"Talanta"},{"key":"10.1016\/j.jpba.2019.02.029_bib0155","series-title":"Methods in Molecular Biology","doi-asserted-by":"crossref","DOI":"10.1007\/978-1-4939-6700-1","author":"Gao","year":"2017"},{"key":"10.1016\/j.jpba.2019.02.029_bib0160","doi-asserted-by":"crossref","first-page":"234","DOI":"10.1016\/j.jprot.2015.04.016","article-title":"Quantitative analysis of post-translational modifications in human serum transthyretin associated with familial amyloidotic polyneuropathy by targeted LC\u2013MS and intact protein MS","volume":"127","author":"Vil\u00e0-Rico","year":"2015","journal-title":"J. Proteom."},{"issue":"7","key":"10.1016\/j.jpba.2019.02.029_bib0165","doi-asserted-by":"crossref","first-page":"633","DOI":"10.1038\/nbt.1546","article-title":"Multi-site assessment of the precision and reproducibility of multiple reaction monitoring-based measurements of proteins in plasma","volume":"27","author":"Addona","year":"2009","journal-title":"Nat. Biotechnol."},{"issue":"10","key":"10.1016\/j.jpba.2019.02.029_bib0170","doi-asserted-by":"crossref","first-page":"2339","DOI":"10.1074\/mcp.M900140-MCP200","article-title":"Quantification of cardiovascular biomarkers in patient plasma by targeted mass spectrometry and stable isotope dilution","volume":"8","author":"Keshishian","year":"2009","journal-title":"Mol. Cell. Proteom. MCP"},{"issue":"5","key":"10.1016\/j.jpba.2019.02.029_bib0175","first-page":"519","article-title":"Comparison of two lowry methods for determination of protein content in bulk of boredella pertussis","volume":"30","author":"Wei","year":"2017","journal-title":"Chin. J. Biol."},{"key":"10.1016\/j.jpba.2019.02.029_bib0180","doi-asserted-by":"crossref","first-page":"899","DOI":"10.1016\/j.colsurfb.2016.06.014","article-title":"Effect of cross-linker glutaraldehyde on gastric digestion of emulsified albumin","volume":"145","author":"del Castillo-Santaella","year":"2016","journal-title":"Colloid. Surf. B"},{"issue":"18","key":"10.1016\/j.jpba.2019.02.029_bib0185","doi-asserted-by":"crossref","first-page":"2129","DOI":"10.1016\/j.vaccine.2016.03.007","article-title":"Effect of different detoxification procedures on the residual pertussis toxin activities in vaccines","volume":"34","author":"Yuen","year":"2016","journal-title":"Vaccine"},{"issue":"19","key":"10.1016\/j.jpba.2019.02.029_bib0190","doi-asserted-by":"crossref","first-page":"2678","DOI":"10.1016\/S0264-410X(03)00105-1","article-title":"Novel configurations of high molecular weight species of the pertussis toxin vaccine component","volume":"21","author":"Fowler","year":"2003","journal-title":"Vaccine"},{"issue":"5","key":"10.1016\/j.jpba.2019.02.029_bib0195","doi-asserted-by":"crossref","first-page":"359","DOI":"10.1016\/0264-410X(95)00230-X","article-title":"The effect of formaldehyde, hydrogen peroxide and genetic detoxification of pertussis toxin on epitope recognition by murine monoclonal antibodies","volume":"14","author":"Ibsen","year":"1996","journal-title":"Vaccine"}],"container-title":["Journal of Pharmaceutical and Biomedical Analysis"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0731708518318296?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0731708518318296?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2022,9,12]],"date-time":"2022-09-12T09:46:57Z","timestamp":1662976017000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0731708518318296"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,5]]},"references-count":39,"alternative-id":["S0731708518318296"],"URL":"https:\/\/doi.org\/10.1016\/j.jpba.2019.02.029","relation":{},"ISSN":["0731-7085"],"issn-type":[{"value":"0731-7085","type":"print"}],"subject":[],"published":{"date-parts":[[2019,5]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Quantitative determination of bioactive proteins in diphtheria tetanus acellular pertussis (DTaP) vaccine by liquid chromatography tandem mass spectrometry","name":"articletitle","label":"Article Title"},{"value":"Journal of Pharmaceutical and Biomedical Analysis","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.jpba.2019.02.029","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2019 Elsevier B.V. All rights reserved.","name":"copyright","label":"Copyright"}]}}