{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,5]],"date-time":"2026-08-05T07:29:45Z","timestamp":1785914985564,"version":"3.56.0"},"reference-count":36,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2020,11,1]],"date-time":"2020-11-01T00:00:00Z","timestamp":1604188800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2020,11,1]],"date-time":"2020-11-01T00:00:00Z","timestamp":1604188800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"}],"funder":[{"DOI":"10.13039\/501100002322","name":"Coordena\u00e7\u00e3o de Aperfei\u00e7oamento de Pessoal de N\u00edvel Superior","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100002322","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Journal of Pharmaceutical and Biomedical Analysis"],"published-print":{"date-parts":[[2020,11]]},"DOI":"10.1016\/j.jpba.2020.113635","type":"journal-article","created":{"date-parts":[[2020,9,17]],"date-time":"2020-09-17T12:48:17Z","timestamp":1600346897000},"page":"113635","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":10,"special_numbering":"C","title":["An enquiry into antileishmanial activity and quantitative analysis of polyhydroxylated steroidal saponins from Solanum paniculatum L. leaves"],"prefix":"10.1016","volume":"191","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0199-4521","authenticated-orcid":false,"given":"Alexander B.","family":"Valerino-D\u00edaz","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ana C.","family":"Zanatta","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daylin","family":"Gamiotea-Turro","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ana Carolina Bolela Bovo","family":"Candido","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lizandra Guidi","family":"Magalh\u00e3es","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wagner","family":"Vilegas","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lourdes Campaner dos","family":"Santos","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.jpba.2020.113635_bib0005","doi-asserted-by":"crossref","first-page":"16","DOI":"10.1016\/j.foodres.2014.01.057","article-title":"Extraction and quantification of saponins: a review","volume":"59","author":"Cheok","year":"2014","journal-title":"Food Res. Int."},{"key":"10.1016\/j.jpba.2020.113635_bib0010","doi-asserted-by":"crossref","first-page":"219","DOI":"10.1016\/j.jep.2004.05.016","article-title":"Biological activities and distribution of plants saponins","volume":"94","author":"Sparg","year":"2004","journal-title":"J. Ethnopharmacol."},{"key":"10.1016\/j.jpba.2020.113635_bib0015","doi-asserted-by":"crossref","first-page":"435","DOI":"10.1016\/j.phytochem.2011.01.015","article-title":"Molecular activities, biosynthesis and evolution of triterpenoid saponins","volume":"72","author":"Augustin","year":"2011","journal-title":"Phytochemistry"},{"key":"10.1016\/j.jpba.2020.113635_bib0020","first-page":"11318","article-title":"Antiproliferation effect and apoptosis mechanism of prostate cancer cells PC-3 by flavonoids and saponins prepared for Gynostemma pentaphyllum","volume":"59","author":"Chen","year":"2011","journal-title":"J. Agric. Food Chem."},{"key":"10.1016\/j.jpba.2020.113635_bib0025","doi-asserted-by":"crossref","first-page":"480","DOI":"10.1016\/j.jcs.2013.02.002","article-title":"Isolation and antioxidative properties of phenolic-saponins rich fraction from deffated rice bran","volume":"57","author":"Chan","year":"2013","journal-title":"J. Cereal Sci."},{"key":"10.1016\/j.jpba.2020.113635_bib0030","doi-asserted-by":"crossref","first-page":"27","DOI":"10.1016\/S0147-9571(99)00020-X","article-title":"Isolation and evaluation of immunological adjuvant activities of saponins from Polygala senega L","volume":"23","author":"Estrada","year":"2000","journal-title":"Comp. Immunol. Microbiol. Infect. Dis."},{"key":"10.1016\/j.jpba.2020.113635_bib0035","doi-asserted-by":"crossref","first-page":"2047","DOI":"10.1016\/j.intimp.2011.08.018","article-title":"Structure \u2013 function relationship of saponins from roots of Platycodon glandiflorum for hemolytic and adjuvant activity","volume":"11","author":"Sun","year":"2011","journal-title":"Int. Immunopharmacol."},{"key":"10.1016\/j.jpba.2020.113635_bib0040","doi-asserted-by":"crossref","first-page":"3113","DOI":"10.1021\/jf205010c","article-title":"Immunoadjuvant activity, toxicity assays, and determination by UPLC\/Q-TOF\/MS of triterpenic saponins from Chenopodium quinoa seeds","volume":"60","author":"Verza","year":"2012","journal-title":"J. Agric. Food Chem."},{"issue":"10","key":"10.1016\/j.jpba.2020.113635_bib0045","doi-asserted-by":"crossref","first-page":"1404","DOI":"10.3390\/molecules21101404","article-title":"Effects of Saponins on Lipid Metabolism: a Review of potential health benefits in the treatment of obesity","volume":"21","author":"Marrelli","year":"2016","journal-title":"Molecules"},{"key":"10.1016\/j.jpba.2020.113635_bib0050","doi-asserted-by":"crossref","first-page":"543","DOI":"10.1039\/c0np00059k","article-title":"Synthesis, biology and clinical significance of pentacyclic triterpenes: a multi-target approach to prevention and treatment of metabolic and vascular diseases","volume":"28","author":"Sheng","year":"2011","journal-title":"Nat. Prod. Rep."},{"key":"10.1016\/j.jpba.2020.113635_bib0055","doi-asserted-by":"crossref","first-page":"77","DOI":"10.1007\/s13659-019-0201-6","article-title":"The genus Solanum: an ethnophamacological, phytochemical and biological properties review","volume":"9","author":"Sakah Kaunda","year":"2019","journal-title":"Nat. Prod. Bioprospect."},{"key":"10.1016\/j.jpba.2020.113635_bib0060","series-title":"Cat\u00e1logo dos Dados Principais sobre Plantas utilizadas em Medicina e Farm\u00e1cia","article-title":"Notas de Fitoterapia","author":"Coimbra","year":"1958"},{"key":"10.1016\/j.jpba.2020.113635_bib0065","first-page":"395","volume":"vol 3","author":"Corr\u00eaa","year":"1984"},{"key":"10.1016\/j.jpba.2020.113635_bib0070","doi-asserted-by":"crossref","first-page":"8703","DOI":"10.1021\/acs.jafc.8b01262","article-title":"New polyhydroxylated steroidal saponins from Solanum paniculatum L. leaf alcohol tincture with antibacterial activity against oral pathogens","volume":"66","author":"Valerino-Diaz","year":"2018","journal-title":"J. Agric. Food Chem."},{"key":"10.1016\/j.jpba.2020.113635_bib0075","doi-asserted-by":"crossref","first-page":"642","DOI":"10.1002\/cbdv.201200063","article-title":"In vitro leishmanicidal and cytotoxic activities of the glycoalkaloids from Solanum lycocarpum (Solanaceae) fruits","volume":"10","author":"Miranda","year":"2013","journal-title":"Chem. Biodivers."},{"key":"10.1016\/j.jpba.2020.113635_bib0080","first-page":"806","article-title":"Leaf epidermal characters of Brazilian species of Solanum section Torva as taxonomic evidence","volume":"58","author":"Nurit-Silva","year":"2012","journal-title":"Can. J. Microbiol."},{"key":"10.1016\/j.jpba.2020.113635_bib0085","doi-asserted-by":"crossref","first-page":"160","DOI":"10.1016\/j.foodchem.2014.08.005","article-title":"New steroidal saponins and antiulcer activity from Solanum paniculatum L","volume":"86","author":"Vieira j\u00fanior","year":"2015","journal-title":"Food Chem."},{"key":"10.1016\/j.jpba.2020.113635_bib0090","unstructured":"World Health Organization (WHO): Global Health Observatory Data 2018. Leishmaniasis. Situation and trends. Available at: https:\/\/www.who.int\/gho\/neglected_diseases\/leishmaniasis\/en\/#:\u223c:text=Out%20of%20200%20countries%20and,endemic%20for%20leishmaniasis%20in%202018. (Accessed 15 August 2020)."},{"key":"10.1016\/j.jpba.2020.113635_bib0095","doi-asserted-by":"crossref","first-page":"239","DOI":"10.1590\/S0074-02762004000300001","article-title":"Clinical and immunopathological spectrum of American cutaneous Leishmaniasis with special reference to the disease in Amazonian Brazil - A review","volume":"99","author":"Silveira","year":"2004","journal-title":"Mem. Inst. Oswaldo Cruz"},{"key":"10.1016\/j.jpba.2020.113635_bib0100","first-page":"1","article-title":"N-acetyl-L-cysteine reduces Leishmania amazonensis-induced inflammation in BALB\/c mice","volume":"16","author":"Crupi","year":"2020","journal-title":"Vet. Res."},{"key":"10.1016\/j.jpba.2020.113635_bib0105","doi-asserted-by":"crossref","first-page":"21","DOI":"10.1016\/j.exppara.2016.03.026","article-title":"Antileishmanial activity and mechanism of action from a purified fraction of Zinziber officinalis Roschoe against Leishmania amazonensis","volume":"166","author":"Duarte","year":"2016","journal-title":"Exp. Parasitol."},{"key":"10.1016\/j.jpba.2020.113635_bib0110","doi-asserted-by":"crossref","first-page":"127","DOI":"10.1002\/ptr.2650040402","article-title":"Natural products and the development of selective antiprotozoal drugs","volume":"4","author":"Wright","year":"2000","journal-title":"Phytother. Res."},{"key":"10.1016\/j.jpba.2020.113635_bib0115","doi-asserted-by":"crossref","first-page":"70","DOI":"10.1016\/j.jep.2016.01.012","article-title":"In vitro evaluation of traditionally used Surinamese medicinal plants for their potential anti-Leishmanial efficacy","volume":"180","author":"Mans","year":"2016","journal-title":"J. Ethnopharmacol."},{"key":"10.1016\/j.jpba.2020.113635_bib0120","doi-asserted-by":"crossref","first-page":"523","DOI":"10.1016\/j.ejmech.2013.02.038","article-title":"Potent naphthoquinones against antimony-sensitive and -resistant Leishmania parasites: Synthesis of novel \u03b1- and nor- \u03b1-lapachonebased 1,2,3-triazoles by copper-catalyzed azide-alkyne cycloaddition","volume":"63","author":"Guimar\u00e3es","year":"2013","journal-title":"Eur. J. Med. Chem."},{"key":"10.1016\/j.jpba.2020.113635_bib0125","doi-asserted-by":"crossref","first-page":"111","DOI":"10.1016\/j.mimet.2013.05.012","article-title":"Improvement of 96-well microplate assay of estimation of cell growth and inibition of Leishmania with Alamar Blue","volume":"94","author":"Corral","year":"2013","journal-title":"J. Microbiol. Methods"},{"key":"10.1016\/j.jpba.2020.113635_bib0130","doi-asserted-by":"crossref","first-page":"2085","DOI":"10.1007\/s00436-018-5894-7","article-title":"Susceptibility of prosmatigotes and intracelular amastigotes from distinct Leishmania species to the calpain inhitor MDL 28170","volume":"117","author":"Souza Ara\u00fajo","year":"2018","journal-title":"Parasitol. Res."},{"key":"10.1016\/j.jpba.2020.113635_bib0135","doi-asserted-by":"crossref","first-page":"12","DOI":"10.1016\/j.exppara.2018.07.003","article-title":"Bovine serum albumin nanoparticles containing amphotericin B were effective in treating murine cutaneous Leishmaniasis and reduced the drug toxicity","volume":"192","author":"Casa","year":"2018","journal-title":"Exp. Parasitol."},{"key":"10.1016\/j.jpba.2020.113635_bib0140","doi-asserted-by":"crossref","first-page":"1079","DOI":"10.1248\/cpb.55.1079","article-title":"Distinction of absolute configuration at C-22 of C-23-hydroxylspirostane and C-23-hydroxyspirosolane glycosides","volume":"5","author":"Matsushita","year":"2007","journal-title":"Chem. Pharm. Bull."},{"key":"10.1016\/j.jpba.2020.113635_bib0145","series-title":"Ag\u00eancia Nacional de Vigil\u00e2ncia Sanit\u00e1ria. Resolu\u00e7\u00e3o da Diretoria Colegiada - RDC N\u00ba 166, 25\/07\/2017","author":"Brasil Anvisa","year":"2017"},{"key":"10.1016\/j.jpba.2020.113635_bib0150","first-page":"271","article-title":"Valida\u00e7\u00e3o de princ\u00edpios ativos de plantas medicinais e fitoter\u00e1picos. Farmacognosia","author":"Marcucci","year":"2011","journal-title":"Colet\u00e2nea Cient\u00edfica"},{"key":"10.1016\/j.jpba.2020.113635_bib0155","doi-asserted-by":"crossref","first-page":"751","DOI":"10.1038\/nrd4683","article-title":"Hit and criteria in drug discovery for infectious diseases of the developing world","volume":"14","author":"Katsuno","year":"2015","journal-title":"Nat. Rev. Drug Discov."},{"key":"10.1016\/j.jpba.2020.113635_bib0160","doi-asserted-by":"crossref","first-page":"495","DOI":"10.1017\/S0031182002001439","article-title":"The role of promastigote secretory gel in the origin and transmission of the infective stage of Leishmania Mexicana by the sandfly Lutzomyia longipalpis","volume":"124","author":"Rogers","year":"2002","journal-title":"Parasitol"},{"key":"10.1016\/j.jpba.2020.113635_bib0165","doi-asserted-by":"crossref","first-page":"531","DOI":"10.1007\/s00436-017-5731-4","article-title":"Assessment of the antiprotozoal activity of Pulicaria inuloides extracts, an Algerian medicinal plant: leishmanicidal bioguided fractionation","volume":"117","author":"Fadel","year":"2018","journal-title":"Parasitol. Res."},{"key":"10.1016\/j.jpba.2020.113635_bib0170","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1186\/s13104-017-3022-x","article-title":"Experimental therapeutic assays of Tephrosia vogelii against Leishmania major infection in murine model: in vitro and in vivo","volume":"10","author":"Marango","year":"2017","journal-title":"BMC Res. Notes"},{"key":"10.1016\/j.jpba.2020.113635_bib0175","doi-asserted-by":"crossref","first-page":"4625","DOI":"10.1007\/s00436-015-4708-4","article-title":"Antileishmanial activity and evaluation of the mechanism of action of strychnobiflavone flavonoid isolated from Strychnos pseudoquina against Leishmania infantum","volume":"114","author":"Lage","year":"2015","journal-title":"Parasitol. Res."},{"key":"10.1016\/j.jpba.2020.113635_bib0180","doi-asserted-by":"crossref","first-page":"e1253","DOI":"10.1371\/journal.pntd.0001253","article-title":"A screen against Leishmania intracellular amastigotes: comparison to a promastigotes screen and identification of a host cell-specific hit","volume":"5","author":"de Muylder","year":"2011","journal-title":"PloS Neglect. Trop. D."}],"container-title":["Journal of Pharmaceutical and Biomedical Analysis"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0731708520315211?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0731708520315211?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,10,9]],"date-time":"2025-10-09T09:12:15Z","timestamp":1760001135000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0731708520315211"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,11]]},"references-count":36,"alternative-id":["S0731708520315211"],"URL":"https:\/\/doi.org\/10.1016\/j.jpba.2020.113635","relation":{},"ISSN":["0731-7085"],"issn-type":[{"value":"0731-7085","type":"print"}],"subject":[],"published":{"date-parts":[[2020,11]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"An enquiry into antileishmanial activity and quantitative analysis of polyhydroxylated steroidal saponins from Solanum paniculatum L. leaves","name":"articletitle","label":"Article Title"},{"value":"Journal of Pharmaceutical and Biomedical Analysis","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.jpba.2020.113635","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2020 Elsevier B.V. All rights reserved.","name":"copyright","label":"Copyright"}],"article-number":"113635"}}