{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T03:23:48Z","timestamp":1790306628397,"version":"4.1.0"},"reference-count":32,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2021,2,1]],"date-time":"2021-02-01T00:00:00Z","timestamp":1612137600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2021,2,1]],"date-time":"2021-02-01T00:00:00Z","timestamp":1612137600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82073974"],"award-info":[{"award-number":["82073974"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81603256"],"award-info":[{"award-number":["81603256"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["21974129"],"award-info":[{"award-number":["21974129"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004826","name":"Natural Science Foundation of Beijing Municipality","doi-asserted-by":"publisher","award":["8192049"],"award-info":[{"award-number":["8192049"]}],"id":[{"id":"10.13039\/501100004826","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012226","name":"Fundamental Research Funds for the Central Universities","doi-asserted-by":"publisher","award":["2019-JYB-TD005"],"award-info":[{"award-number":["2019-JYB-TD005"]}],"id":[{"id":"10.13039\/501100012226","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012226","name":"Fundamental Research Funds for the Central Universities","doi-asserted-by":"publisher","award":["BUCM-2019-JCRC002"],"award-info":[{"award-number":["BUCM-2019-JCRC002"]}],"id":[{"id":"10.13039\/501100012226","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012226","name":"Fundamental Research Funds for the Central Universities","doi-asserted-by":"publisher","award":["2020-JYB-ZDGG-044"],"award-info":[{"award-number":["2020-JYB-ZDGG-044"]}],"id":[{"id":"10.13039\/501100012226","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100005090","name":"Beijing Nova Program","doi-asserted-by":"publisher","award":["Z201100006820026"],"award-info":[{"award-number":["Z201100006820026"]}],"id":[{"id":"10.13039\/501100005090","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Journal of Pharmaceutical and Biomedical Analysis"],"published-print":{"date-parts":[[2021,2]]},"DOI":"10.1016\/j.jpba.2020.113820","type":"journal-article","created":{"date-parts":[[2020,12,2]],"date-time":"2020-12-02T11:53:15Z","timestamp":1606909995000},"page":"113820","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":50,"special_numbering":"C","title":["Comprehensive analysis of Huanglian Jiedu decoction: Revealing the presence of a self-assembled phytochemical complex in its naturally-occurring precipitate"],"prefix":"10.1016","volume":"195","author":[{"given":"Meng","family":"Chen","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Penglong","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tong","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Linsen","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Junfang","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hua","family":"Bai","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Haimin","family":"Lei","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7887-2031","authenticated-orcid":false,"given":"Qiang","family":"Ma","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.jpba.2020.113820_bib0005","series-title":"Serum Pharmacochemistry of Traditional Chinese Medicine: Technologies, Strategies and Applications","first-page":"23","article-title":"Chapter 4 - UPLC\/MS and its potential in traditional Chinese medicine development","author":"Zhang","year":"2017"},{"key":"10.1016\/j.jpba.2020.113820_bib0010","series-title":"Comprehensive Natural Products II: Chemistry and Biology","first-page":"383","article-title":"3.13 - Chinese traditional medicine","author":"Yang","year":"2010"},{"key":"10.1016\/j.jpba.2020.113820_bib0015","series-title":"Systems Biology and its Application in TCM Formulas Research","first-page":"3","article-title":"Chapter 1 - strategy for modern research of traditional Chinese medicine formulae","author":"Yan","year":"2018"},{"key":"10.1016\/j.jpba.2020.113820_bib0020","series-title":"Serum Pharmacochemistry of Traditional Chinese Medicine: Technologies, Strategies and Applications","first-page":"1","article-title":"Chapter 1 - traditional Chinese medicine: current state, challenges, and applications","author":"Wang","year":"2017"},{"issue":"1","key":"10.1016\/j.jpba.2020.113820_bib0025","doi-asserted-by":"crossref","first-page":"57","DOI":"10.1186\/s13020-019-0277-2","article-title":"Huang-Lian Jie-Du decoction: a review on phytochemical, pharmacological and pharmacokinetic investigations","volume":"14","author":"Qi","year":"2019","journal-title":"Chin. Med."},{"issue":"4","key":"10.1016\/j.jpba.2020.113820_bib0030","doi-asserted-by":"crossref","first-page":"227","DOI":"10.4103\/2225-4110.119732","article-title":"The anticancer properties and apoptosis-inducing mechanisms of cinnamaldehyde and the herbal prescription Huang-Lian-Jie-Du-Tang (Hu\u00e1ng Li\u00e1n Ji\u011b D\u00fa Tang) in human hepatoma cells","volume":"3","author":"Lin","year":"2013","journal-title":"J. Tradit. Complementary Med."},{"issue":"1","key":"10.1016\/j.jpba.2020.113820_bib0035","doi-asserted-by":"crossref","first-page":"55","DOI":"10.1016\/j.jep.2014.03.007","article-title":"Metabolomic analysis of biochemical changes in the plasma and urine of collagen-induced arthritis in rats after treatment with Huang-Lian-Jie-Du-Tang","volume":"154","author":"Zhang","year":"2014","journal-title":"J. Ethnopharmacol."},{"issue":"1","key":"10.1016\/j.jpba.2020.113820_bib0040","first-page":"30","article-title":"In silico target fishing for the potential bioactive components contained in Huanglian Jiedu Tang (HLJDD) and elucidating molecular mechanisms for the treatment of sepsis","volume":"13","author":"Ma","year":"2015","journal-title":"Chin. J. Nat. Med."},{"issue":"92","key":"10.1016\/j.jpba.2020.113820_bib0045","doi-asserted-by":"crossref","first-page":"89855","DOI":"10.1039\/C6RA17380B","article-title":"Huang-Lian-Jie-Du decoction treated sepsis via regulating ERK and SRC\/STAT3 pathways and ameliorating metabolic status","volume":"6","author":"Liao","year":"2016","journal-title":"RSC Adv."},{"issue":"81","key":"10.1016\/j.jpba.2020.113820_bib0050","doi-asserted-by":"crossref","first-page":"66200","DOI":"10.1039\/C5RA12224D","article-title":"Cholestatic liver injury model of bile duct ligation and the protection of Huang-Lian-Jie-Du decoction by NMR metabolomic profiling","volume":"5","author":"Wei","year":"2015","journal-title":"RSC Adv."},{"issue":"2","key":"10.1016\/j.jpba.2020.113820_bib0055","doi-asserted-by":"crossref","first-page":"221","DOI":"10.1002\/2211-5463.12178","article-title":"Protection by Huang-Lian-Jie-Du decoction and its constituent herbs of lipopolysaccharide-induced acute kidney injury","volume":"7","author":"Li","year":"2017","journal-title":"FEBS Open Bio"},{"key":"10.1016\/j.jpba.2020.113820_bib0060","doi-asserted-by":"crossref","first-page":"147","DOI":"10.1016\/j.jep.2018.01.015","article-title":"Metabolomics-based mechanisms exploration of Huang-Lian Jie-Du decoction on cerebral ischemia via UPLC-Q-TOF\/MS analysis on rat serum","volume":"216","author":"Zhu","year":"2018","journal-title":"J. Ethnopharmacol."},{"issue":"5","key":"10.1016\/j.jpba.2020.113820_bib0065","doi-asserted-by":"crossref","first-page":"615","DOI":"10.1016\/j.phymed.2013.11.004","article-title":"Hypolipidemic effect of the Chinese polyherbal Huanglian Jiedu decoction in type 2 diabetic rats and its possible mechanism","volume":"21","author":"Zhang","year":"2014","journal-title":"Phytomedicine"},{"issue":"3","key":"10.1016\/j.jpba.2020.113820_bib0070","doi-asserted-by":"crossref","first-page":"e92954","DOI":"10.1371\/journal.pone.0092954","article-title":"Effects of Huanglian-Jie-Du-Tang and its modified formula on the modulation of amyloid-\u03b2 precursor protein processing in Alzheimer\u2019s disease models","volume":"9","author":"Durairajan","year":"2014","journal-title":"PLoS One"},{"key":"10.1016\/j.jpba.2020.113820_bib0075","doi-asserted-by":"crossref","first-page":"75","DOI":"10.1016\/j.jpba.2017.12.053","article-title":"Explore the effects of Huang-Lian-Jie-Du-Tang on Alzheimer\u2019s disease by UPLC-QTOF\/MS-based plasma metabolomics study","volume":"151","author":"Sun","year":"2018","journal-title":"J. Pharm. Biomed. Anal."},{"key":"10.1016\/j.jpba.2020.113820_bib0080","series-title":"Chinese Herbal Formulas: Treatment Principles and Composition Strategies","first-page":"1","article-title":"Theories and concepts in the composition of Chinese herbal formulas","author":"Yang","year":"2010"},{"issue":"1","key":"10.1016\/j.jpba.2020.113820_bib0085","doi-asserted-by":"crossref","first-page":"165","DOI":"10.1016\/S1570-0232(04)00579-3","article-title":"Study on the precipitation reaction between baicalin and berberine by HPLC","volume":"810","author":"Li","year":"2004","journal-title":"J. Chromatogr. B"},{"issue":"1","key":"10.1016\/j.jpba.2020.113820_bib0090","doi-asserted-by":"crossref","first-page":"128","DOI":"10.1016\/j.jep.2013.02.019","article-title":"Study on intestinal absorption and pharmacokinetic characterization of diester diterpenoid alkaloids in precipitation derived from Fuzi\u2013Gancao herb-pair decoction for its potential interaction mechanism investigation","volume":"147","author":"Zhang","year":"2013","journal-title":"J. Ethnopharmacol."},{"issue":"9","key":"10.1016\/j.jpba.2020.113820_bib0095","doi-asserted-by":"crossref","first-page":"1784","DOI":"10.1016\/j.apsb.2019.12.014","article-title":"Self-assembled natural phytochemicals for synergistically antibacterial application from the enlightenment of traditional Chinese medicine combination","volume":"10","author":"Tian","year":"2019","journal-title":"Acta Pharm. Sin. B"},{"issue":"1","key":"10.1016\/j.jpba.2020.113820_bib0100","doi-asserted-by":"crossref","first-page":"227","DOI":"10.1021\/acsami.9b17722","article-title":"Self-assemblies based on traditional medicine berberine and cinnamic acid for adhesion-induced inhibition multidrug-resistant Staphylococcus aureus","volume":"12","author":"Huang","year":"2020","journal-title":"ACS Appl. Mater. Interfaces"},{"issue":"8","key":"10.1016\/j.jpba.2020.113820_bib0105","doi-asserted-by":"crossref","first-page":"1094","DOI":"10.3390\/molecules21081094","article-title":"Compositions, formation mechanism, and neuroprotective effect of compound precipitation from the traditional Chinese prescription Huang-Lian-Jie-Du-Tang","volume":"21","author":"Zhang","year":"2016","journal-title":"Molecules"},{"issue":"6","key":"10.1016\/j.jpba.2020.113820_bib0110","doi-asserted-by":"crossref","first-page":"6770","DOI":"10.1021\/acsnano.9b01346","article-title":"Natural berberine-based Chinese herb medicine assembled nanostructures with modified antibacterial application","volume":"13","author":"Li","year":"2019","journal-title":"ACS Nano"},{"issue":"9","key":"10.1016\/j.jpba.2020.113820_bib0115","doi-asserted-by":"crossref","first-page":"1456","DOI":"10.3390\/molecules22091456","article-title":"Origin and formation mechanism investigation of compound precipitation from the traditional Chinese prescription Huang-Lian-Jie-Du-Tang by isothermal titration calorimetry","volume":"22","author":"Wang","year":"2017","journal-title":"Molecules"},{"key":"10.1016\/j.jpba.2020.113820_bib0120","doi-asserted-by":"crossref","first-page":"239","DOI":"10.1016\/j.jpba.2013.07.033","article-title":"Quality evaluation of commercial Huang-Lian-Jie-Du-Tang based on simultaneous determination of fourteen major chemical constituents using high performance liquid chromatography","volume":"85","author":"Kwok","year":"2013","journal-title":"J. Pharm. Biomed. Anal."},{"key":"10.1016\/j.jpba.2020.113820_bib0125","doi-asserted-by":"crossref","first-page":"659","DOI":"10.1365\/s10337-008-0945-3","article-title":"LC\u2013DAD and LC\u2013ESI\u2013MS chromatographic fingerprinting and quantitative analysis for evaluation of the quality of Huang-Lian-Jie-Du-Tang","volume":"69","author":"Dou","year":"2009","journal-title":"Chromatographia"},{"key":"10.1016\/j.jpba.2020.113820_bib0130","doi-asserted-by":"crossref","first-page":"1015","DOI":"10.1248\/bpb.22.1015","article-title":"Simultaneous determination of baicalin, wogonoside, baicalein, wogonin, berberine, coptisine, palmatine, jateorrhizine and glycyrrhizin in kampo medicines by ion-pair high-performance liquid chromatography","volume":"22","author":"Okamura","year":"1999","journal-title":"Biol. Pharm. Bull."},{"issue":"3","key":"10.1016\/j.jpba.2020.113820_bib0135","doi-asserted-by":"crossref","first-page":"355","DOI":"10.1248\/bpb.35.355","article-title":"Huanglian-Jie-Du-Tang extract protects against chronic brain injury after focal cerebral ischemia via hypoxia-inducible-factor-1\u03b1-regulated vascular endothelial growth factor signaling in mice","volume":"35","author":"Ye","year":"2012","journal-title":"Biol. Pharm. Bull."},{"key":"10.1016\/j.jpba.2020.113820_bib0140","doi-asserted-by":"crossref","first-page":"88","DOI":"10.1016\/j.chroma.2013.10.072","volume":"1321","author":"Yang","year":"2013","journal-title":"J. Chromatogr. A"},{"issue":"20","key":"10.1016\/j.jpba.2020.113820_bib0145","doi-asserted-by":"crossref","first-page":"4357","DOI":"10.1039\/c1an15444c","article-title":"Selective separation of structure-related alkaloids in Rhizoma coptidis with \u201cclick\u201d binaphthyl stationary phase and their structural elucidation with liquid chromatography-mass spectrometry","volume":"136","author":"Liu","year":"2011","journal-title":"Analyst"},{"issue":"7","key":"10.1016\/j.jpba.2020.113820_bib0150","doi-asserted-by":"crossref","first-page":"1103","DOI":"10.2116\/analsci.20.1103","article-title":"Structural analysis of selected characteristic flavones by electrospray tandem mass spectrometry","volume":"20","author":"Wu","year":"2004","journal-title":"Anal. Sci."},{"issue":"17","key":"10.1016\/j.jpba.2020.113820_bib0155","doi-asserted-by":"crossref","first-page":"2520","DOI":"10.1002\/rcm.4643","article-title":"Fragmentation study of iridoid glycosides including epimers by liquid chromatography\u2010diode array detection\/electrospray ionization mass spectrometry and its application in metabolic fingerprint analysis of Gardenia jasminoides Ellis","volume":"24","author":"Zhou","year":"2010","journal-title":"Rapid Commun. Mass Spectrom."},{"issue":"7","key":"10.1016\/j.jpba.2020.113820_bib0160","first-page":"712A","article-title":"Limit of detection a closer look at the IUPAC definition","volume":"55","author":"Long","year":"1983","journal-title":"Anal. Chem."}],"container-title":["Journal of Pharmaceutical and Biomedical Analysis"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0731708520317064?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0731708520317064?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,4,30]],"date-time":"2026-04-30T18:06:15Z","timestamp":1777572375000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0731708520317064"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,2]]},"references-count":32,"alternative-id":["S0731708520317064"],"URL":"https:\/\/doi.org\/10.1016\/j.jpba.2020.113820","relation":{},"ISSN":["0731-7085"],"issn-type":[{"value":"0731-7085","type":"print"}],"subject":[],"published":{"date-parts":[[2021,2]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Comprehensive analysis of Huanglian Jiedu decoction: Revealing the presence of a self-assembled phytochemical complex in its naturally-occurring precipitate","name":"articletitle","label":"Article Title"},{"value":"Journal of Pharmaceutical and Biomedical Analysis","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.jpba.2020.113820","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2020 Elsevier B.V. All rights reserved.","name":"copyright","label":"Copyright"}],"article-number":"113820"}}