{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,18]],"date-time":"2026-09-18T01:15:23Z","timestamp":1789694123152,"version":"4.0.1"},"reference-count":19,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2022,1,1]],"date-time":"2022-01-01T00:00:00Z","timestamp":1640995200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2022,1,1]],"date-time":"2022-01-01T00:00:00Z","timestamp":1640995200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2022,1,1]],"date-time":"2022-01-01T00:00:00Z","timestamp":1640995200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-017"},{"start":{"date-parts":[[2022,1,1]],"date-time":"2022-01-01T00:00:00Z","timestamp":1640995200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2022,1,1]],"date-time":"2022-01-01T00:00:00Z","timestamp":1640995200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-012"},{"start":{"date-parts":[[2022,1,1]],"date-time":"2022-01-01T00:00:00Z","timestamp":1640995200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2022,1,1]],"date-time":"2022-01-01T00:00:00Z","timestamp":1640995200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-004"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82003927"],"award-info":[{"award-number":["82003927"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Journal of Pharmaceutical and Biomedical Analysis"],"published-print":{"date-parts":[[2022,1]]},"DOI":"10.1016\/j.jpba.2021.114431","type":"journal-article","created":{"date-parts":[[2021,10,14]],"date-time":"2021-10-14T13:11:55Z","timestamp":1634217115000},"page":"114431","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":16,"special_numbering":"C","title":["Metabolic profiling and pharmacokinetic studies of sinapine thiocyanate by UHPLC-Q\/TOF-MS and UHPLC-MS\/MS"],"prefix":"10.1016","volume":"207","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9198-883X","authenticated-orcid":false,"given":"Huida","family":"Guan","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qiyan","family":"Lin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chao","family":"Ma","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhengcai","family":"Ju","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Changhong","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"issue":"6","key":"10.1016\/j.jpba.2021.114431_bib1","doi-asserted-by":"crossref","first-page":"3637","DOI":"10.1039\/C9FO00195F","article-title":"Sinapine reduces non-alcoholic fatty liver disease in mice by modulating the composition of the gut microbiota","volume":"10","author":"Li","year":"2019","journal-title":"Food Funct."},{"key":"10.1016\/j.jpba.2021.114431_bib2","doi-asserted-by":"crossref","DOI":"10.1016\/j.redox.2020.101554","article-title":"Sinapine, but not sinapic acid, counteracts mitochondrial oxidative stress in cardiomyocytes","volume":"34","author":"Boulghobra","year":"2020","journal-title":"Redox Biol."},{"key":"10.1016\/j.jpba.2021.114431_bib3","doi-asserted-by":"crossref","first-page":"768","DOI":"10.1016\/j.foodchem.2018.10.045","article-title":"Determination of sinapine in rapeseed pomace extract: Its antioxidant and acetylcholinesterase inhibition properties","volume":"276","author":"Yates","year":"2019","journal-title":"Food Chem."},{"key":"10.1016\/j.jpba.2021.114431_bib4","series-title":"Pharmacopoeia of the People\u2019s Republic of China","first-page":"1","author":"Chinese Pharmacopeia Commission","year":"2020"},{"key":"10.1016\/j.jpba.2021.114431_bib5","doi-asserted-by":"crossref","first-page":"196","DOI":"10.1016\/j.phymed.2018.05.010","article-title":"Comparison of the anti-inflammatory effects of Sinapis alba and Brassica juncea in mouse models of inflammation","volume":"50","author":"Xian","year":"2018","journal-title":"Phytomedicine"},{"key":"10.1016\/j.jpba.2021.114431_bib6","doi-asserted-by":"crossref","DOI":"10.1155\/2013\/636194","article-title":"A review of the phytochemistry and pharmacological activities of raphani semen","volume":"2013","author":"Sham","year":"2013","journal-title":"Evid. Based Complement. Altern. Med."},{"issue":"1","key":"10.1016\/j.jpba.2021.114431_bib7","first-page":"134","article-title":"Effect of sinapine on relieving asthma and its mechanism","volume":"42","author":"Wang","year":"2011","journal-title":"Chin. Tradit. Herb. Drugs"},{"issue":"4","key":"10.1016\/j.jpba.2021.114431_bib8","doi-asserted-by":"crossref","first-page":"237","DOI":"10.5582\/bst.2015.01087","article-title":"Protective effects on vascular endothelial cell in N\u2032-nitro-L-arginine (l-NNA)-induced hypertensive rats from the combination of effective components of Uncaria rhynchophylla and Semen Raphani","volume":"9","author":"Li","year":"2015","journal-title":"Biosci. Trends"},{"issue":"6","key":"10.1016\/j.jpba.2021.114431_bib9","doi-asserted-by":"crossref","first-page":"3081","DOI":"10.3892\/etm.2017.4357","article-title":"Mechanism of the protective effects of the combined treatment with rhynchophylla total alkaloids and sinapine thiocyanate against a prothrombotic state caused by vascular endothelial cell inflammatory damage","volume":"13","author":"Li","year":"2017","journal-title":"Exp. Ther. Med."},{"key":"10.1016\/j.jpba.2021.114431_bib10","article-title":"Sinapine thiocyanate ameliorates vascular endothelial dysfunction in hypertension by inhibiting activation of the NLRP3 inflammasome","volume":"11","author":"Liu","year":"2020","journal-title":"Front. Pharmacol."},{"key":"10.1016\/j.jpba.2021.114431_bib11","doi-asserted-by":"crossref","DOI":"10.1016\/j.jpba.2021.113957","article-title":"Rapid screening of bioactive compound in Sanzi Yangqin Decoction and investigating of binding mechanism by immobilized \u03b2(2)-adrenogic receptor chromatography coupled with molecular docking","volume":"197","author":"Wang","year":"2021","journal-title":"J. Pharm. Biomed. Anal."},{"issue":"10","key":"10.1016\/j.jpba.2021.114431_bib12","doi-asserted-by":"crossref","first-page":"628","DOI":"10.1002\/jmr.2478","article-title":"Target-directed screening of the bioactive compounds specifically binding to \u03b22-adrenoceptor in Semen brassicae by high-performance affinity chromatography","volume":"28","author":"An","year":"2015","journal-title":"J. Mol. Recognit."},{"issue":"9","key":"10.1016\/j.jpba.2021.114431_bib13","doi-asserted-by":"crossref","first-page":"1345","DOI":"10.1021\/tx200168d","article-title":"Structural alert\/reactive metabolite concept as applied in medicinal chemistry to mitigate the risk of idiosyncratic drug toxicity: a perspective based on the critical examination of trends in the top 200 drugs marketed in the United States","volume":"24","author":"Stepan","year":"2011","journal-title":"Chem. Res. Toxicol."},{"issue":"3","key":"10.1016\/j.jpba.2021.114431_bib14","first-page":"531","article-title":"Determination of five components in Pingfei oral liquid by HPLC","volume":"39","author":"Xiao","year":"2019","journal-title":"Chin. J. Pharm. Anal."},{"issue":"1","key":"10.1016\/j.jpba.2021.114431_bib15","doi-asserted-by":"crossref","first-page":"136","DOI":"10.1016\/j.ijpharm.2013.09.015","article-title":"Skin penetration of topically applied white mustard extract and its effects on epidermal Langerhans cells and cytokines","volume":"457","author":"Guo","year":"2013","journal-title":"Int. J. Pharm."},{"key":"10.1016\/j.jpba.2021.114431_bib16","doi-asserted-by":"crossref","first-page":"56","DOI":"10.1016\/j.jchromb.2018.05.014","article-title":"Systematic screening and characterization of Qi-Li-Qiang-Xin capsule-related xenobiotics in rats by ultra-performance liquid chromatography coupled with quadrupole time-of-flight tandem mass spectrometry","volume":"1090","author":"Yun","year":"2018","journal-title":"J. Chromatogr. B. Anal. Technol. Biomed. Life Sci."},{"key":"10.1016\/j.jpba.2021.114431_bib17","doi-asserted-by":"crossref","first-page":"9502902","DOI":"10.1155\/2018\/9502902","article-title":"In vivo study on site of action of sinapine thiocyanate following acupoint herbal patching","volume":"2018","author":"Chen","year":"2018","journal-title":"Evid. Based Complement. Altern. Med."},{"issue":"2","key":"10.1016\/j.jpba.2021.114431_bib18","first-page":"336","article-title":"Dermal pharmacokinetic study of Baijiezi Tufang by microdialysis combined with RP-HPLC","volume":"43","author":"Xu","year":"2012","journal-title":"Chin. Tradit. Herb. Drugs"},{"key":"10.1016\/j.jpba.2021.114431_bib19","unstructured":"F.D. Administration, USFDA, Guidance for Industry: Bioanalytical Method Validation, \u3008http:\/\/www.fda.gov.\/cder\/guidance\/4252fnl.pdf\u3009, 66, 2001."}],"container-title":["Journal of Pharmaceutical and Biomedical Analysis"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0731708521005422?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0731708521005422?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,10,9]],"date-time":"2025-10-09T09:19:18Z","timestamp":1760001558000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0731708521005422"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,1]]},"references-count":19,"alternative-id":["S0731708521005422"],"URL":"https:\/\/doi.org\/10.1016\/j.jpba.2021.114431","relation":{},"ISSN":["0731-7085"],"issn-type":[{"value":"0731-7085","type":"print"}],"subject":[],"published":{"date-parts":[[2022,1]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Metabolic profiling and pharmacokinetic studies of sinapine thiocyanate by UHPLC-Q\/TOF-MS and UHPLC-MS\/MS","name":"articletitle","label":"Article Title"},{"value":"Journal of Pharmaceutical and Biomedical Analysis","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.jpba.2021.114431","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2021 Elsevier B.V. All rights reserved.","name":"copyright","label":"Copyright"}],"article-number":"114431"}}