{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,15]],"date-time":"2026-09-15T11:00:51Z","timestamp":1789470051179,"version":"build-2803163510"},"reference-count":36,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2024,1,1]],"date-time":"2024-01-01T00:00:00Z","timestamp":1704067200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2024,1,1]],"date-time":"2024-01-01T00:00:00Z","timestamp":1704067200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2024,1,1]],"date-time":"2024-01-01T00:00:00Z","timestamp":1704067200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-017"},{"start":{"date-parts":[[2024,1,1]],"date-time":"2024-01-01T00:00:00Z","timestamp":1704067200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2024,1,1]],"date-time":"2024-01-01T00:00:00Z","timestamp":1704067200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-012"},{"start":{"date-parts":[[2024,1,1]],"date-time":"2024-01-01T00:00:00Z","timestamp":1704067200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2024,1,1]],"date-time":"2024-01-01T00:00:00Z","timestamp":1704067200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-004"}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Journal of Pharmaceutical and Biomedical Analysis"],"published-print":{"date-parts":[[2024,1]]},"DOI":"10.1016\/j.jpba.2023.115813","type":"journal-article","created":{"date-parts":[[2023,11,2]],"date-time":"2023-11-02T17:42:49Z","timestamp":1698946969000},"page":"115813","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":1,"special_numbering":"C","title":["Development of a free cytokine immunoassay to maintain binding and dissociation equilibrium in vitro"],"prefix":"10.1016","volume":"238","author":[{"ORCID":"https:\/\/orcid.org\/0009-0006-2108-0910","authenticated-orcid":false,"given":"Siqi","family":"Li","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hao","family":"Guo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yan","family":"Gao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wen","family":"Tian","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shan","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chen","family":"Shen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lili","family":"Xu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hailong","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jinliang","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yingwu","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"issue":"5","key":"10.1016\/j.jpba.2023.115813_bib1","doi-asserted-by":"crossref","first-page":"589","DOI":"10.1093\/ajcp\/105.5.589","article-title":"Cytokine function: a study in biologic diversity","volume":"105","author":"Cohen","year":"1996","journal-title":"Am. J. Clin. Pathol."},{"issue":"2","key":"10.1016\/j.jpba.2023.115813_bib2","doi-asserted-by":"crossref","first-page":"149","DOI":"10.1002\/jlb.67.2.149","article-title":"Extracellular matrix moieties cytokines and enzymes: dynamic effects on immune cell behavior and inflammation","volume":"67","author":"Vaday","year":"2000","journal-title":"J. Leukoc. Biol."},{"key":"10.1016\/j.jpba.2023.115813_bib3","doi-asserted-by":"crossref","first-page":"1","DOI":"10.3389\/fimmu.2014.00491","article-title":"Macrophage cytokines: involvement in immunity and infectious diseases","volume":"5","author":"Arango Duque","year":"2014","journal-title":"Front. Immunol."},{"key":"10.1016\/j.jpba.2023.115813_bib4","volume":"127\u2013155","author":"O'Shea","year":"2019","journal-title":"Cytokines Cytokine Recept."},{"key":"10.1016\/j.jpba.2023.115813_bib5","doi-asserted-by":"crossref","unstructured":"Meng Pengyu Chen Walker Ting Chung Robert McHugh Yuwei Nidetz Jianping Li Timothy T. Fu Thomas P. Cornell Katsuo Shanley Kurabayashi, multiplex serum cytokine immunoassay using nanoplasmonic biosensor microarrays ACS Nano 9 4 2015 4173 4181.","DOI":"10.1021\/acsnano.5b00396"},{"issue":"15","key":"10.1016\/j.jpba.2023.115813_bib6","doi-asserted-by":"crossref","DOI":"10.1002\/advs.202004433","article-title":"Cytokines: from clinical significance to quantification","volume":"8","author":"Liu","year":"2021","journal-title":"Adv. Sci."},{"issue":"5","key":"10.1016\/j.jpba.2023.115813_bib7","doi-asserted-by":"crossref","first-page":"1583","DOI":"10.1016\/S0002-9440(10)63415-3","article-title":"Rheumatic heart disease: proinflammatory cytokines play a role in the progression and maintenance of valvular lesions","volume":"165","author":"Guilherme","year":"2004","journal-title":"Am. J. Pathol."},{"issue":"2","key":"10.1016\/j.jpba.2023.115813_bib8","doi-asserted-by":"crossref","first-page":"R49","DOI":"10.1186\/cc5783","article-title":"Cytokine profiles as markers of disease severity in sepsis: a multiplex analysis","volume":"11","author":"Bozza","year":"2007","journal-title":"Crit. Care"},{"issue":"6","key":"10.1016\/j.jpba.2023.115813_bib9","doi-asserted-by":"crossref","first-page":"429","DOI":"10.1038\/nri2094","article-title":"Cytokines in the pathogenesis of rheumatoid arthritis","volume":"7","author":"McInnes","year":"2007","journal-title":"Nat. Rev. Immunol."},{"issue":"2","key":"10.1016\/j.jpba.2023.115813_bib10","doi-asserted-by":"crossref","first-page":"215","DOI":"10.1016\/j.cell.2008.07.001","article-title":"TGFbeta in Cancer","volume":"134","author":"Massague","year":"2008","journal-title":"Cell"},{"issue":"5","key":"10.1016\/j.jpba.2023.115813_bib11","doi-asserted-by":"crossref","first-page":"446","DOI":"10.1016\/j.biopsych.2009.09.033","article-title":"A meta-analysis of cytokines in major depression","volume":"67","author":"Dowlati","year":"2010","journal-title":"Biol. Psychiatry"},{"key":"10.1016\/j.jpba.2023.115813_bib12","doi-asserted-by":"crossref","first-page":"697","DOI":"10.1056\/NEJMoa050580","article-title":"Evidence of a role of tumor necrosis factor \u03b1 in Refractory Asthma","volume":"354","author":"Berry","year":"2006","journal-title":"New Engl. J. Med."},{"issue":"1","key":"10.1016\/j.jpba.2023.115813_bib13","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1016\/j.atherosclerosis.2004.11.016","article-title":"Disruption of tumor necrosis factor-alpha gene diminishes the development of atherosclerosis in ApoE-deficient mice","volume":"180","author":"Ohta","year":"2005","journal-title":"Atherosclerosis"},{"issue":"7","key":"10.1016\/j.jpba.2023.115813_bib14","doi-asserted-by":"crossref","first-page":"791","DOI":"10.1038\/ki.2011.1","article-title":"The meaning of urinary creatinine concentration","volume":"79","author":"Ortiz","year":"2011","journal-title":"Kidney Int."},{"issue":"6","key":"10.1016\/j.jpba.2023.115813_bib15","doi-asserted-by":"crossref","first-page":"511","DOI":"10.1016\/S1359-6101(03)00058-3","article-title":"Cytokine-directed therapies for the treatment of chronic airway diseases","volume":"14","author":"Barnes","year":"2003","journal-title":"Cytokine Growth Factor Rev."},{"key":"10.1016\/j.jpba.2023.115813_bib16","doi-asserted-by":"crossref","first-page":"4923062","DOI":"10.1155\/2018\/4923062","article-title":"Baicalin downregulates RLRs signaling pathway to control influenza A virus infection and improve the prognosis","volume":"2018","author":"Pang","year":"2018","journal-title":"Evid. Based Complement Altern. Med."},{"issue":"4","key":"10.1016\/j.jpba.2023.115813_bib17","doi-asserted-by":"crossref","first-page":"267","DOI":"10.1515\/CCLM.2000.040","article-title":"The clinical usefulness of the measurement of cytokines","volume":"38","author":"Bienvenu","year":"2000","journal-title":"Clin. Chem. Lab. Med."},{"issue":"3","key":"10.1016\/j.jpba.2023.115813_bib18","doi-asserted-by":"crossref","first-page":"2551","DOI":"10.1021\/acs.analchem.8b05892","article-title":"Ultrasensitive and simultaneous detection of two cytokines secreted by single cell in microfluidic droplets via magnetic-field amplified SERS","volume":"91","author":"Sun","year":"2019","journal-title":"Anal. Chem."},{"key":"10.1016\/j.jpba.2023.115813_bib19","article-title":"Detection of cytokine mRNA expression by PCR","author":"James","year":"2001","journal-title":"Curr. Protoc. Immunol."},{"key":"10.1016\/j.jpba.2023.115813_bib20","doi-asserted-by":"crossref","DOI":"10.3389\/fimmu.2021.723585","article-title":"Different profiles of antibodies and cytokines were found between severe and moderate COVID-19 patients","volume":"12","author":"Guo","year":"2021","journal-title":"Front. Immunol."},{"key":"10.1016\/j.jpba.2023.115813_bib21","first-page":"65","article-title":"Luminex xMAP assay to quantify cytokines in cancer patient serum","volume":"108","author":"Kupcova Skalnikova","year":"2020","journal-title":"lMethods Mol. Biol."},{"key":"10.1016\/j.jpba.2023.115813_bib22","doi-asserted-by":"crossref","DOI":"10.1016\/j.cyto.2020.155272","article-title":"Biosensors: a novel approach to and recent discovery in detection of cytokines","volume":"136","author":"Mobed","year":"2020","journal-title":"Cytokine"},{"issue":"4","key":"10.1016\/j.jpba.2023.115813_bib23","doi-asserted-by":"crossref","DOI":"10.1002\/VIW.20210003","article-title":"Point-of-care detection of cytokines in cytokine storm management and beyond: significance and challenges","volume":"2","author":"Liu","year":"2021","journal-title":"View"},{"key":"10.1016\/j.jpba.2023.115813_bib24","doi-asserted-by":"crossref","first-page":"2014","DOI":"10.3389\/fimmu.2019.02014","article-title":"Interleukin-1 family cytokines: keystones in liver inflammatory diseases","volume":"10","author":"Barbier","year":"2019","journal-title":"Front Immunol."},{"issue":"8","key":"10.1016\/j.jpba.2023.115813_bib25","doi-asserted-by":"crossref","first-page":"633","DOI":"10.1038\/nrd3800","article-title":"Treating inflammation by blocking interleukin-1 in a broad spectrum of diseases","volume":"11","author":"Dinarello","year":"2012","journal-title":"Nat. Rev. Drug Discov."},{"key":"10.1016\/j.jpba.2023.115813_bib26","doi-asserted-by":"crossref","first-page":"118","DOI":"10.1159\/000092969","article-title":"The inflammatory tumor microenvironment and its impact on cancer development","volume":"13","author":"de Vissera","year":"2006","journal-title":"Contrib. Microbiol."},{"issue":"3","key":"10.1016\/j.jpba.2023.115813_bib27","doi-asserted-by":"crossref","first-page":"897","DOI":"10.1016\/j.jpba.2008.07.012","article-title":"A novel method for quantitative measurement of a biomarker in the presence of a therapeutic monoclonal antibody directed against the biomarker","volume":"48","author":"Davis","year":"2008","journal-title":"J. Pharm. Biomed. Anal."},{"issue":"6","key":"10.1016\/j.jpba.2023.115813_bib28","doi-asserted-by":"crossref","first-page":"1125","DOI":"10.4155\/bio.10.64","article-title":"Therapeutic monoclonal antibody concentration monitoring free or total?","volume":"2","author":"Kuang","year":"2010","journal-title":"Bioanalysis"},{"issue":"1","key":"10.1016\/j.jpba.2023.115813_bib29","doi-asserted-by":"crossref","first-page":"99","DOI":"10.1208\/s12248-011-9251-3","article-title":"Bioanalytical approaches to quantify \"total\" and \"free\" therapeutic antibodies and their targets: technical challenges and PK\/PD applications over the course of drug development","volume":"13","author":"Lee","year":"2011","journal-title":"AAPS J."},{"issue":"11","key":"10.1016\/j.jpba.2023.115813_bib30","doi-asserted-by":"crossref","first-page":"1163","DOI":"10.4155\/bio.11.73","article-title":"Free versus total ligand-binding assays: points to consider in biotherapeutic drug development","volume":"3","author":"Yang","year":"2011","journal-title":"Bioanalysis"},{"issue":"11","key":"10.1016\/j.jpba.2023.115813_bib31","doi-asserted-by":"crossref","first-page":"1287","DOI":"10.4155\/bio.11.104","article-title":"Application and interpretation of free and total drug measurements in the development of biologics","volume":"3","author":"Ahene","year":"2011","journal-title":"Bioanalysis"},{"issue":"2","key":"10.1016\/j.jpba.2023.115813_bib32","doi-asserted-by":"crossref","first-page":"459","DOI":"10.1067\/mob.2001.115106","article-title":"Optimization of cytokine stability in stored amniotic fluid","volume":"185","author":"Porter","year":"2001","journal-title":"Am. J. Obstet. Gynecol."},{"key":"10.1016\/j.jpba.2023.115813_bib33","doi-asserted-by":"crossref","first-page":"453","DOI":"10.1016\/j.cyto.2018.06.009","article-title":"Effect of storage duration on cytokine stability in human serum and plasma","volume":"113","author":"Vincent","year":"2019","journal-title":"Cytokine"},{"key":"10.1016\/j.jpba.2023.115813_bib34","doi-asserted-by":"crossref","first-page":"38","DOI":"10.1016\/j.clinbiochem.2019.01.001","article-title":"Impact of clinical sample handling and processing on ultra-low level measurements of plasma cytokines","volume":"65","author":"Cohen","year":"2019","journal-title":"Clin. Biochem."},{"key":"10.1016\/j.jpba.2023.115813_bib35","doi-asserted-by":"crossref","DOI":"10.1016\/j.cyto.2019.154829","article-title":"Thermal stability of cytokines: a review","volume":"125","author":"Simpson","year":"2020","journal-title":"Cytokine"},{"issue":"12","key":"10.1016\/j.jpba.2023.115813_bib36","doi-asserted-by":"crossref","first-page":"2030","DOI":"10.1002\/alz.12342","article-title":"Knowledge gaps in Alzheimer's disease immune biomarker research","volume":"17","author":"Morgan","year":"2021","journal-title":"Alzheimers Dement"}],"container-title":["Journal of Pharmaceutical and Biomedical Analysis"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0731708523005824?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0731708523005824?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,10,9]],"date-time":"2025-10-09T09:33:41Z","timestamp":1760002421000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0731708523005824"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,1]]},"references-count":36,"alternative-id":["S0731708523005824"],"URL":"https:\/\/doi.org\/10.1016\/j.jpba.2023.115813","relation":{},"ISSN":["0731-7085"],"issn-type":[{"value":"0731-7085","type":"print"}],"subject":[],"published":{"date-parts":[[2024,1]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Development of a free cytokine immunoassay to maintain binding and dissociation equilibrium in vitro","name":"articletitle","label":"Article Title"},{"value":"Journal of Pharmaceutical and Biomedical Analysis","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.jpba.2023.115813","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2023 Elsevier B.V. All rights reserved.","name":"copyright","label":"Copyright"}],"article-number":"115813"}}