{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,1]],"date-time":"2026-02-01T01:31:39Z","timestamp":1769909499983,"version":"3.49.0"},"reference-count":38,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2024,8,1]],"date-time":"2024-08-01T00:00:00Z","timestamp":1722470400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2024,8,1]],"date-time":"2024-08-01T00:00:00Z","timestamp":1722470400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2024,8,1]],"date-time":"2024-08-01T00:00:00Z","timestamp":1722470400000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-017"},{"start":{"date-parts":[[2024,8,1]],"date-time":"2024-08-01T00:00:00Z","timestamp":1722470400000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2024,8,1]],"date-time":"2024-08-01T00:00:00Z","timestamp":1722470400000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-012"},{"start":{"date-parts":[[2024,8,1]],"date-time":"2024-08-01T00:00:00Z","timestamp":1722470400000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2024,8,1]],"date-time":"2024-08-01T00:00:00Z","timestamp":1722470400000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-004"}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Journal of Pharmaceutical and Biomedical Analysis"],"published-print":{"date-parts":[[2024,8]]},"DOI":"10.1016\/j.jpba.2024.116180","type":"journal-article","created":{"date-parts":[[2024,4,29]],"date-time":"2024-04-29T05:50:41Z","timestamp":1714369841000},"page":"116180","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":3,"special_numbering":"C","title":["Sequence confirmation of synthetic DNA exceeding 100 nucleotides using restriction enzyme mediated digestion combined with high-resolution tandem mass spectrometry"],"prefix":"10.1016","volume":"245","author":[{"given":"Christian","family":"Sattler","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Burak","family":"Ceylan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Luisa","family":"Hoffmann","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andela","family":"Juric","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Julia","family":"Kraus","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shima","family":"Marandi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Aref","family":"Shahnazari","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michael","family":"R\u00fchl","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1016\/j.jpba.2024.116180_bib1","doi-asserted-by":"crossref","first-page":"145","DOI":"10.3390\/ph14020145","article-title":"2020 FDA TIDES (Peptides and Oligonucleotides) Harvest","volume":"14","author":"Al Musaimi","year":"2021","journal-title":"Pharmaceuticals"},{"key":"10.1016\/j.jpba.2024.116180_bib2","doi-asserted-by":"crossref","first-page":"222","DOI":"10.3390\/ph15020222","article-title":"2021 FDA TIDES (Peptides and Oligonucleotides) Harvest","volume":"15","author":"Al Shaer","year":"2022","journal-title":"Pharmaceuticals"},{"key":"10.1016\/j.jpba.2024.116180_bib3","doi-asserted-by":"crossref","first-page":"630","DOI":"10.1038\/s41565-021-00898-0","article-title":"The current landscape of nucleic acid therapeutics","volume":"16","author":"Kulkarni","year":"2021","journal-title":"Nat. Nanotechnol."},{"key":"10.1016\/j.jpba.2024.116180_bib4","doi-asserted-by":"crossref","first-page":"890","DOI":"10.1212\/WNL.0000000000002445","article-title":"Results from a phase 1 study of nusinersen (ISIS-SMNRx) in children with spinal muscular atrophy","volume":"86","author":"Chiriboga","year":"2016","journal-title":"Neurology"},{"key":"10.1016\/j.jpba.2024.116180_bib5","doi-asserted-by":"crossref","first-page":"277","DOI":"10.1007\/s40265-020-01463-0","article-title":"Lumasiran: First Approval","volume":"81","author":"Scott","year":"2021","journal-title":"Drugs"},{"key":"10.1016\/j.jpba.2024.116180_bib6","unstructured":"The new frontier of genome engineering with CRISPR-Cas9 | Science, (n.d.). https:\/\/www.science.org\/doi\/abs\/10.1126\/science.1258096 (accessed January 24, 2023).."},{"key":"10.1016\/j.jpba.2024.116180_bib7","doi-asserted-by":"crossref","first-page":"2150","DOI":"10.1016\/j.apsb.2021.05.020","article-title":"In vivo delivery of CRISPR-Cas9 therapeutics: progress and challenges","volume":"11","author":"Behr","year":"2021","journal-title":"Acta Pharm. Sin. B"},{"key":"10.1016\/j.jpba.2024.116180_bib8","doi-asserted-by":"crossref","first-page":"947","DOI":"10.1177\/08971900211009650","article-title":"Review of COVID-19 mRNA Vaccines: BNT162b2 and mRNA-1273","volume":"35","author":"Teo","year":"2022","journal-title":"J. Pharm. Pract."},{"key":"10.1016\/j.jpba.2024.116180_bib9","doi-asserted-by":"crossref","DOI":"10.1016\/j.ijpharm.2021.120586","article-title":"mRNA-lipid nanoparticle COVID-19 vaccines: structure and stability","volume":"601","author":"Schoenmaker","year":"2021","journal-title":"Int. J. Pharm."},{"key":"10.1016\/j.jpba.2024.116180_bib10","doi-asserted-by":"crossref","first-page":"317","DOI":"10.1007\/s40259-017-0234-5","article-title":"Adeno-Associated Virus (AAV) as a Vector for Gene Therapy","volume":"31","author":"Naso","year":"2017","journal-title":"BioDrugs"},{"key":"10.1016\/j.jpba.2024.116180_bib11","doi-asserted-by":"crossref","first-page":"601","DOI":"10.1146\/annurev-virology-092818-015530","article-title":"Recombinant Adeno-Associated Virus Gene Therapy in Light of Luxturna (and Zolgensma and Glybera): where are we, and how did we get here?","volume":"6","author":"Keeler","year":"2019","journal-title":"Annu. Rev. Virol."},{"key":"10.1016\/j.jpba.2024.116180_bib12","doi-asserted-by":"crossref","first-page":"1001","DOI":"10.1177\/1060028020914274","article-title":"Onasemnogene Abeparvovec-xioi: gene therapy for spinal muscular atrophy","volume":"54","author":"Stevens","year":"2020","journal-title":"Ann. Pharmacother."},{"key":"10.1016\/j.jpba.2024.116180_bib13","doi-asserted-by":"crossref","first-page":"1450","DOI":"10.3390\/microorganisms10071450","article-title":"Viral vector vaccine development and application during the COVID-19 pandemic","volume":"10","author":"Deng","year":"2022","journal-title":"Microorganisms"},{"key":"10.1016\/j.jpba.2024.116180_bib14","doi-asserted-by":"crossref","first-page":"1075","DOI":"10.1080\/14712598.2022.2066471","article-title":"Onasemnogene abeparvovec for the treatment of spinal muscular atrophy","volume":"22","author":"McMillan","year":"2022","journal-title":"Expert Opin. Biol. Ther."},{"key":"10.1016\/j.jpba.2024.116180_bib15","doi-asserted-by":"crossref","first-page":"918","DOI":"10.1016\/j.jasms.2010.02.025","article-title":"Top-down mass spectrometry for sequencing of larger (up to 61 nt) RNA by CAD and EDD","volume":"21","author":"Taucher","year":"2010","journal-title":"J. Am. Soc. Mass Spectrom."},{"key":"10.1016\/j.jpba.2024.116180_bib16","doi-asserted-by":"crossref","DOI":"10.1016\/1044-0305(92)85019-G","article-title":"Tandem mass spectrometry of small, multiply charged oligonucleotides","author":"Mcluckey","year":"1992","journal-title":"J. Am. Soc. Mass Spectrom."},{"key":"10.1016\/j.jpba.2024.116180_bib17","doi-asserted-by":"crossref","first-page":"740","DOI":"10.1016\/1044-0305(94)80006-5","article-title":"Ion trap tandem mass spectrometry applied to small multiply charged oligonucleotides with a modified base","volume":"5","author":"McLuckey","year":"1994","journal-title":"J. Am. Soc. Mass Spectrom."},{"key":"10.1016\/j.jpba.2024.116180_bib18","doi-asserted-by":"crossref","first-page":"364","DOI":"10.1002\/jms.2044","article-title":"Collision-induced dissociation of oligonucleotide anions fully modified at the 2\u2019-position of the ribose: 2\u2019-F\/-H and 2\u2019-F\/-H\/-OMe mix-mers","volume":"47","author":"Gao","year":"2012","journal-title":"J. Mass Spectrom."},{"key":"10.1016\/j.jpba.2024.116180_bib19","doi-asserted-by":"crossref","DOI":"10.1002\/jms.4819","article-title":"Tandem mass spectrometric sequence characterization of synthetic thymidine-rich oligonucleotides","volume":"57","author":"Abdullah","year":"2022","journal-title":"J. Mass Spectrom."},{"key":"10.1016\/j.jpba.2024.116180_bib20","doi-asserted-by":"crossref","first-page":"3173","DOI":"10.1021\/ac900100x","article-title":"Sequencing of single and double stranded rna oligonucleotides by acid hydrolysis and MALDI mass spectrometry","volume":"81","author":"Bahr","year":"2009","journal-title":"Anal. Chem."},{"key":"10.1016\/j.jpba.2024.116180_bib21","unstructured":"Sequencing of Phosphoramidate Oligonucleotides by Acid Hydrolysis and Mass Spectrometry | Analytical Chemistry, (n.d.). \u3008https:\/\/pubs.acs.org\/doi\/abs\/10.1021\/acs.analchem.9b01858\u3009 (accessed January 24, 2023)."},{"key":"10.1016\/j.jpba.2024.116180_bib22","doi-asserted-by":"crossref","first-page":"2631","DOI":"10.1002\/rcm.4683","article-title":"Ultra-performance liquid chromatography\/tandem mass spectrometry (UPLC\/MS\/MS) and UPLC\/MSE analysis of RNA oligonucleotides","volume":"24","author":"Ivleva","year":"2010","journal-title":"Rapid Commun. Mass Spectrom."},{"key":"10.1016\/j.jpba.2024.116180_bib23","doi-asserted-by":"crossref","first-page":"14569","DOI":"10.1021\/acs.analchem.9b03625","article-title":"Absolute quantification of RNA or DNA using acid hydrolysis and mass spectrometry","volume":"91","author":"Lowenthal","year":"2019","journal-title":"Anal. Chem."},{"key":"10.1016\/j.jpba.2024.116180_bib24","doi-asserted-by":"crossref","first-page":"9352","DOI":"10.1021\/ja9533611","article-title":"Sequence information from 42\u2212108-mer DNAs (Complete for a 50-mer) by Tandem Mass Spectrometry","volume":"118","author":"Little","year":"1996","journal-title":"J. Am. Chem. Soc."},{"key":"10.1016\/j.jpba.2024.116180_bib25","doi-asserted-by":"crossref","DOI":"10.1021\/acs.analchem.2c03030","article-title":"Top-down mass spectrometry of synthetic single guide ribonucleic acids enabled by facile sample clean-up","author":"Crittenden","year":"2023","journal-title":"Anal. Chem."},{"key":"10.1016\/j.jpba.2024.116180_bib26","doi-asserted-by":"crossref","first-page":"14792","DOI":"10.1021\/acs.analchem.1c03533","article-title":"Full Sequencing of CRISPR\/Cas9 Single Guide RNA (sgRNA) via Parallel ribonuclease digestions and hydrophilic interaction liquid chromatography\u2013high-resolution mass spectrometry analysis","volume":"93","author":"Goyon","year":"2021","journal-title":"Anal. Chem."},{"key":"10.1016\/j.jpba.2024.116180_bib27","doi-asserted-by":"crossref","first-page":"1169","DOI":"10.1021\/acs.analchem.1c04350","article-title":"On-line sequencing of CRISPR guide RNAs and their impurities via the use of immobilized ribonuclease cartridges attached to a 2D\/3D-LC\u2013MS system","volume":"94","author":"Goyon","year":"2022","journal-title":"Anal. Chem."},{"key":"10.1016\/j.jpba.2024.116180_bib28","doi-asserted-by":"crossref","first-page":"2809","DOI":"10.1007\/s00216-023-04693-9","article-title":"Middle-out sequence confirmation of CRISPR\/Cas9 single guide RNA (sgRNA) using DNA primers and ribonuclease T1 digestion","volume":"415","author":"Chin","year":"2023","journal-title":"Anal. Bioanal. Chem."},{"key":"10.1016\/j.jpba.2024.116180_bib29","doi-asserted-by":"crossref","first-page":"7339","DOI":"10.1021\/acs.analchem.2c00765","article-title":"Characterization and sequence mapping of large RNA and mRNA therapeutics using mass spectrometry","volume":"94","author":"Vanhinsbergh","year":"2022","journal-title":"Anal. Chem."},{"key":"10.1016\/j.jpba.2024.116180_bib30","doi-asserted-by":"crossref","DOI":"10.1093\/nar\/gkac632","article-title":"Human RNase 4 improves mRNA sequence characterization by LC\u2013MS\/MS","volume":"50","author":"Wolf","year":"2022","journal-title":"Nucleic Acids Res"},{"key":"10.1016\/j.jpba.2024.116180_bib31","doi-asserted-by":"crossref","DOI":"10.1093\/nar\/gni099","article-title":"Real-time monitoring of enzymatic DNA hydrolysis by electrospray ionization mass spectrometry","volume":"33","author":"van den Heuvel","year":"2005","journal-title":"Nucleic Acids Res."},{"key":"10.1016\/j.jpba.2024.116180_bib32","doi-asserted-by":"crossref","first-page":"28","DOI":"10.1016\/j.ab.2018.01.027","article-title":"Microwave-assisted enzymatic hydrolysis of DNA for mass spectrometric analysis: a new strategy for accelerated hydrolysis","volume":"546","author":"Chilakala","year":"2018","journal-title":"Anal. Biochem."},{"key":"10.1016\/j.jpba.2024.116180_bib33","doi-asserted-by":"crossref","first-page":"666","DOI":"10.1016\/j.tig.2018.05.008","article-title":"The third revolution in sequencing technology","volume":"34","author":"van Dijk","year":"2018","journal-title":"Trends Genet"},{"key":"10.1016\/j.jpba.2024.116180_bib34","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1016\/j.csbj.2019.11.002","article-title":"Long walk to genomics: history and current approaches to genome sequencing and assembly","volume":"18","author":"Giani","year":"2020","journal-title":"Comput. Struct. Biotechnol. J."},{"key":"10.1016\/j.jpba.2024.116180_bib35","doi-asserted-by":"crossref","first-page":"358","DOI":"10.1038\/s41573-019-0012-9","article-title":"Adeno-associated virus vector as a platform for gene therapy delivery","volume":"18","author":"Wang","year":"2019","journal-title":"Nat. Rev. Drug Discov."},{"key":"10.1016\/j.jpba.2024.116180_bib36","doi-asserted-by":"crossref","first-page":"1859","DOI":"10.1016\/S0040-4039(01)90461-7","article-title":"Deoxynucleoside phosphoramidites\u2014A new class of key intermediates for deoxypolynucleotide synthesis","volume":"22","author":"Beaucage","year":"1981","journal-title":"Tetrahedron Lett."},{"key":"10.1016\/j.jpba.2024.116180_bib37","doi-asserted-by":"crossref","first-page":"1169","DOI":"10.3390\/molecules28031169","article-title":"Investigation of the influence of charge state and collision energy on oligonucleotide fragmentation by tandem mass spectrometry","volume":"28","author":"Gawlig","year":"2023","journal-title":"Molecules"},{"key":"10.1016\/j.jpba.2024.116180_bib38","doi-asserted-by":"crossref","first-page":"2296","DOI":"10.1021\/jasms.3c00207","article-title":"Integrating internal fragments in the interpretation of top-down sequencing data of larger oligonucleotides","volume":"34","author":"Kenderdine","year":"2023","journal-title":"J. Am. Soc. Mass Spectrom."}],"container-title":["Journal of Pharmaceutical and Biomedical Analysis"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0731708524002206?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0731708524002206?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2024,7,10]],"date-time":"2024-07-10T23:47:46Z","timestamp":1720655266000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0731708524002206"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,8]]},"references-count":38,"alternative-id":["S0731708524002206"],"URL":"https:\/\/doi.org\/10.1016\/j.jpba.2024.116180","relation":{},"ISSN":["0731-7085"],"issn-type":[{"value":"0731-7085","type":"print"}],"subject":[],"published":{"date-parts":[[2024,8]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Sequence confirmation of synthetic DNA exceeding 100 nucleotides using restriction enzyme mediated digestion combined with high-resolution tandem mass spectrometry","name":"articletitle","label":"Article Title"},{"value":"Journal of Pharmaceutical and Biomedical Analysis","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.jpba.2024.116180","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2024 Elsevier B.V. All rights reserved.","name":"copyright","label":"Copyright"}],"article-number":"116180"}}