{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,25]],"date-time":"2026-03-25T21:20:51Z","timestamp":1774473651013,"version":"3.50.1"},"reference-count":35,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2025,8,1]],"date-time":"2025-08-01T00:00:00Z","timestamp":1754006400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2025,8,1]],"date-time":"2025-08-01T00:00:00Z","timestamp":1754006400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2025,8,1]],"date-time":"2025-08-01T00:00:00Z","timestamp":1754006400000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-017"},{"start":{"date-parts":[[2025,8,1]],"date-time":"2025-08-01T00:00:00Z","timestamp":1754006400000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2025,8,1]],"date-time":"2025-08-01T00:00:00Z","timestamp":1754006400000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-012"},{"start":{"date-parts":[[2025,8,1]],"date-time":"2025-08-01T00:00:00Z","timestamp":1754006400000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2025,8,1]],"date-time":"2025-08-01T00:00:00Z","timestamp":1754006400000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-004"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81001260"],"award-info":[{"award-number":["81001260"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100017594","name":"Medical Science and Technology Project of Zhejiang Province","doi-asserted-by":"publisher","award":["2020KY451"],"award-info":[{"award-number":["2020KY451"]}],"id":[{"id":"10.13039\/501100017594","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100017594","name":"Medical Science and Technology Project of Zhejiang Province","doi-asserted-by":"publisher","award":["2017KY222"],"award-info":[{"award-number":["2017KY222"]}],"id":[{"id":"10.13039\/501100017594","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100017594","name":"Medical Science and Technology Project of Zhejiang Province","doi-asserted-by":"publisher","award":["2022KY598"],"award-info":[{"award-number":["2022KY598"]}],"id":[{"id":"10.13039\/501100017594","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Journal of Pharmaceutical and Biomedical Analysis"],"published-print":{"date-parts":[[2025,8]]},"DOI":"10.1016\/j.jpba.2025.116800","type":"journal-article","created":{"date-parts":[[2025,3,5]],"date-time":"2025-03-05T03:02:04Z","timestamp":1741143724000},"page":"116800","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":1,"special_numbering":"C","title":["Development and validation of a HPLC-MS\/MS method\u00a0the determination of genistein and equol in serum, urine and follicular fluid"],"prefix":"10.1016","volume":"260","author":[{"given":"Xia","family":"Zheng","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yue-jin","family":"Wu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Li-mei","family":"Wu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ling","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lin","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhen","family":"Jin","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fang","family":"Gao","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qing-qing","family":"Li","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yin","family":"Wang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yi-dan","family":"Wu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"issue":"6","key":"10.1016\/j.jpba.2025.116800_bib1","doi-asserted-by":"crossref","DOI":"10.3390\/molecules24061076","article-title":"Isoflavones","volume":"24","author":"K\u0159\u00ed\u017eov\u00e1","year":"2019","journal-title":"Molecules"},{"issue":"14","key":"10.1016\/j.jpba.2025.116800_bib2","doi-asserted-by":"crossref","DOI":"10.3390\/foods12142665","article-title":"An updated review of soy-derived beverages: nutrition, processing, and bioactivity","volume":"12","author":"Ol\u00edas","year":"2023","journal-title":"Foods"},{"issue":"7-8","key":"10.1016\/j.jpba.2025.116800_bib3","doi-asserted-by":"crossref","first-page":"628","DOI":"10.1016\/j.jchromb.2010.01.028","article-title":"Simultaneous determination of glucuronic acid and sulfuric acid conjugated metabolites of daidzein and genistein in human plasma by high-performance liquid chromatography","volume":"878","author":"Hosoda","year":"2010","journal-title":"J. Chromatogr. B"},{"issue":"7","key":"10.1016\/j.jpba.2025.116800_bib4","doi-asserted-by":"crossref","DOI":"10.3390\/antiox10071064","article-title":"Current perspectives on the beneficial effects of soybean isoflavones and their metabolites for humans","volume":"10","author":"Kim","year":"2021","journal-title":"Antioxidants"},{"issue":"1","key":"10.1016\/j.jpba.2025.116800_bib5","doi-asserted-by":"crossref","DOI":"10.1155\/2021\/3268136","article-title":"Genistein: an integrative overview of its mode of action, pharmacological properties, and health benefits","volume":"2021","author":"Sharifi-Rad","year":"2021","journal-title":"Oxid. Med. Cell. Longev."},{"issue":"2","key":"10.1016\/j.jpba.2025.116800_bib6","doi-asserted-by":"crossref","DOI":"10.3390\/antiox12020368","article-title":"The role of isoflavones in the prevention of breast cancer and prostate cancer","volume":"12","author":"Pej\u010di\u0107","year":"2023","journal-title":"Antioxidants"},{"issue":"7","key":"10.1016\/j.jpba.2025.116800_bib7","doi-asserted-by":"crossref","first-page":"1369S","DOI":"10.3945\/jn.109.118323","article-title":"Emerging research on equol and cancer","volume":"140","author":"Lampe","year":"2010","journal-title":"J. Nutr."},{"issue":"6","key":"10.1016\/j.jpba.2025.116800_bib8","doi-asserted-by":"crossref","DOI":"10.3390\/ijms22063212","article-title":"Utilization of isoflavones in soybeans for women with menopausal syndrome: an overview","volume":"22","author":"Chen","year":"2021","journal-title":"Int. J. Mol. Sci."},{"issue":"18","key":"10.1016\/j.jpba.2025.116800_bib9","doi-asserted-by":"crossref","first-page":"8800","DOI":"10.1039\/D1FO00684C","article-title":"Genistein exhibits therapeutic potential for PCOS mice via the ER-Nrf2-Foxo1-ROS pathway","volume":"12","author":"Luo","year":"2021","journal-title":"Food Funct."},{"issue":"1","key":"10.1016\/j.jpba.2025.116800_bib10","doi-asserted-by":"crossref","first-page":"7204","DOI":"10.1080\/21655979.2021.1979864","article-title":"Soy isoflavones alleviate polycystic ovary syndrome in rats by regulating NF- \u03baB signaling pathway","volume":"12","author":"Ma","year":"2021","journal-title":"Bioengineered"},{"key":"10.1016\/j.jpba.2025.116800_bib11","doi-asserted-by":"crossref","DOI":"10.1016\/j.ejphar.2022.175275","article-title":"Potential roles of genistein in polycystic ovary syndrome: a comprehensive systematic review","volume":"933","author":"Nasimi Doost Azgomi","year":"2022","journal-title":"Eur. J. Pharmacol."},{"issue":"21","key":"10.1016\/j.jpba.2025.116800_bib12","doi-asserted-by":"crossref","DOI":"10.3390\/molecules28217436","article-title":"The role of genistein in mammalian reproduction","volume":"28","author":"Guelfi","year":"2023","journal-title":"Molecules"},{"issue":"5","key":"10.1016\/j.jpba.2025.116800_bib13","doi-asserted-by":"crossref","first-page":"1594","DOI":"10.1111\/jpn.13131","article-title":"Effect of genistein on IGF-1 and IGFBP-1 in young and aged female rat ovary","volume":"103","author":"Chi","year":"2019","journal-title":"J. Anim. Physiol. Anim. Nutr."},{"key":"10.1016\/j.jpba.2025.116800_bib14","doi-asserted-by":"crossref","first-page":"53","DOI":"10.1016\/j.taap.2015.12.026","article-title":"Genistein exposure inhibits growth and alters steroidogenesis in adult mouse antral follicles","volume":"293","author":"Patel","year":"2016","journal-title":"Toxicol. Appl. Pharmacol."},{"issue":"3","key":"10.1016\/j.jpba.2025.116800_bib15","doi-asserted-by":"crossref","first-page":"425","DOI":"10.1095\/biolreprod.108.073171","article-title":"Neonatal exposure to genistein disrupts ability of female mouse reproductive tract to support preimplantation embryo development and implantation1","volume":"80","author":"Jefferson","year":"2009","journal-title":"Biol. Reprod."},{"issue":"6","key":"10.1016\/j.jpba.2025.116800_bib16","doi-asserted-by":"crossref","first-page":"1021","DOI":"10.1095\/biolreprod.109.082941","article-title":"Formation of the ovarian follicular antrum and follicular Fluid1","volume":"82","author":"Rodgers","year":"2010","journal-title":"Biol. Reprod."},{"key":"10.1016\/j.jpba.2025.116800_bib17","doi-asserted-by":"crossref","first-page":"68","DOI":"10.1016\/j.jprot.2013.05.017","article-title":"Proteomic analysis of human follicular fluid: a new perspective towards understanding folliculogenesis","volume":"87","author":"Ambekar","year":"2013","journal-title":"J. Proteom."},{"issue":"49","key":"10.1016\/j.jpba.2025.116800_bib18","doi-asserted-by":"crossref","first-page":"12941","DOI":"10.1021\/acs.jafc.8b04852","article-title":"Metabolite identification and pharmacokinetic profiling of isoflavones from black soybean in rats using ultrahigh-performance liquid chromatography with linear-ion-trap\u2013orbitrap and triple-quadrupole tandem mass spectrometry","volume":"66","author":"Zhang","year":"2018","journal-title":"J. Agric. Food Chem."},{"issue":"24","key":"10.1016\/j.jpba.2025.116800_bib19","doi-asserted-by":"crossref","first-page":"6007","DOI":"10.1007\/s00216-014-8034-y","article-title":"Quantification of soy isoflavones and their conjugative metabolites in plasma and urine: an automated and validated UHPLC-MS\/MS method for use in large-scale studies","volume":"406","author":"Soukup","year":"2014","journal-title":"Anal. Bioanal. Chem."},{"issue":"1-2","key":"10.1016\/j.jpba.2025.116800_bib20","doi-asserted-by":"crossref","first-page":"138","DOI":"10.1016\/j.jchromb.2007.03.011","article-title":"High throughput quantification of phytoestrogens in human urine and serum using liquid chromatography\/tandem mass spectrometry (LC\u2013MS\/MS)","volume":"853","author":"Grace","year":"2007","journal-title":"J. Chromatogr. B"},{"issue":"31","key":"10.1016\/j.jpba.2025.116800_bib21","doi-asserted-by":"crossref","first-page":"6946","DOI":"10.1021\/acs.jafc.5b02891","article-title":"Pharmacokinetic comparison of soy isoflavone extracts in human plasma","volume":"63","author":"Rodr\u00edguez-Morat\u00f3","year":"2015","journal-title":"J. Agric. Food Chem."},{"issue":"13","key":"10.1016\/j.jpba.2025.116800_bib22","doi-asserted-by":"crossref","DOI":"10.3390\/ijms241310852","article-title":"Phytoestrogens present in follicular fluid and urine are positively associated with ivf outcomes following single euploid embryo transfer","volume":"24","author":"Gonzalez-Martin","year":"2023","journal-title":"Int. J. Mol. Sci."},{"key":"10.1016\/j.jpba.2025.116800_bib23","unstructured":"U.S. Food and Drug Administration, Bioanalytical method validation guidance for industry. \u3008https:\/\/www.fda.gov\/files\/drugs\/published\/Bioanalytical-Method-Validation-Guidance-for-Industry.pdf\u3009."},{"key":"10.1016\/j.jpba.2025.116800_bib24","doi-asserted-by":"crossref","first-page":"337","DOI":"10.1016\/j.talanta.2018.05.053","article-title":"Recent developments and applications of different sorbents for SPE and SPME from biological samples","volume":"187","author":"Hashemi","year":"2018","journal-title":"Talanta"},{"key":"10.1016\/j.jpba.2025.116800_bib25","doi-asserted-by":"crossref","DOI":"10.1016\/j.chroma.2022.463049","article-title":"Hybrid silica material as a mixed-mode sorbent for solid-phase extraction of hydrophobic and hydrophilic illegal additives from food samples","volume":"1672","author":"Duan","year":"2022","journal-title":"J. Chromatogr. A"},{"issue":"12","key":"10.1016\/j.jpba.2025.116800_bib26","doi-asserted-by":"crossref","first-page":"1269","DOI":"10.1002\/jms.231","article-title":"Effect of eluent on the ionization efficiency of flavonoids by ion spray, atmospheric pressure chemical ionization, and atmospheric pressure photoionization mass spectrometry","volume":"36","author":"Rauha","year":"2001","journal-title":"J. Mass Spectrom."},{"issue":"8","key":"10.1016\/j.jpba.2025.116800_bib27","doi-asserted-by":"crossref","first-page":"845","DOI":"10.1002\/jms.659","article-title":"Triple quadrupole linear ion trap mass spectrometer for the analysis of small molecules and macromolecules","volume":"39","author":"Hopfgartner","year":"2004","journal-title":"J. Mass Spectrom."},{"key":"10.1016\/j.jpba.2025.116800_bib28","doi-asserted-by":"crossref","first-page":"1466S","DOI":"10.1093\/ajcn\/68.6.1466S","article-title":"Y, Isoflavones in human breast milk and other biological fluids","volume":"68","author":"Franke","year":"1998","journal-title":"Am. J. Clin. Nutr."},{"issue":"13-14","key":"10.1016\/j.jpba.2025.116800_bib29","doi-asserted-by":"crossref","first-page":"949","DOI":"10.1016\/j.jchromb.2010.02.022","article-title":"Development of a high-throughput LC\/APCI-MS method for the determination of thirteen phytoestrogens including gut microbial metabolites in human urine and serum","volume":"878","author":"Wyns","year":"2010","journal-title":"J. Chromatogr. B Anal. Technol. Biomed. Life Sci."},{"issue":"4","key":"10.1016\/j.jpba.2025.116800_bib30","doi-asserted-by":"crossref","first-page":"461","DOI":"10.1080\/01635580801911761","article-title":"Orally administered isoflavones are present as glucuronides in the human prostate","volume":"60","author":"Guy","year":"2008","journal-title":"Nutr. Cancer"},{"issue":"1","key":"10.1016\/j.jpba.2025.116800_bib31","doi-asserted-by":"crossref","DOI":"10.1016\/S1570-0232(02)00789-4","article-title":"D, Quantitation of soy-derived phytoestrogens in human breast tissue and biological fluids by high-performance liquid chromatography","volume":"784","author":"Maubach","year":"2003","journal-title":"J. Chromatogr. B Anal. Technol. Biomed. Life Sci."},{"issue":"3","key":"10.1016\/j.jpba.2025.116800_bib32","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0288946","article-title":"Comparison of blood and urine concentrations of equol by LC-MS\/MS method and factors associated with equol production in 466 Japanese men and women","volume":"19","author":"Yoshikata","year":"2024","journal-title":"Plos One"},{"issue":"7","key":"10.1016\/j.jpba.2025.116800_bib33","doi-asserted-by":"crossref","first-page":"1355S","DOI":"10.3945\/jn.109.119776","article-title":"Equol: History, Chemistry, and Formation","volume":"140","author":"Setchell","year":"2010","journal-title":"J. Nutr."},{"issue":"5","key":"10.1016\/j.jpba.2025.116800_bib34","doi-asserted-by":"crossref","first-page":"1284","DOI":"10.3945\/ajcn.111.019638","article-title":"Soy isoflavone phase II metabolism differs between rodents and humans: implications for the effect on breast cancer risk","volume":"94","author":"Setchell","year":"2011","journal-title":"Am. J. Clin. Nutr."},{"issue":"6","key":"10.1016\/j.jpba.2025.116800_bib35","doi-asserted-by":"crossref","first-page":"1559","DOI":"10.1016\/j.bmc.2003.11.035","article-title":"Equol, a natural estrogenic metabolite from soy isoflavones","volume":"12","author":"Muthyala","year":"2004","journal-title":"Bioorg. Med. Chem."}],"container-title":["Journal of Pharmaceutical and Biomedical Analysis"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0731708525001414?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0731708525001414?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,11,5]],"date-time":"2025-11-05T02:13:21Z","timestamp":1762308801000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0731708525001414"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,8]]},"references-count":35,"alternative-id":["S0731708525001414"],"URL":"https:\/\/doi.org\/10.1016\/j.jpba.2025.116800","relation":{},"ISSN":["0731-7085"],"issn-type":[{"value":"0731-7085","type":"print"}],"subject":[],"published":{"date-parts":[[2025,8]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Development and validation of a HPLC-MS\/MS method\u00a0the determination of genistein and equol in serum, urine and follicular fluid","name":"articletitle","label":"Article Title"},{"value":"Journal of Pharmaceutical and Biomedical Analysis","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.jpba.2025.116800","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2025 Elsevier B.V. All rights are reserved, including those for text and data mining, AI training, and similar technologies.","name":"copyright","label":"Copyright"}],"article-number":"116800"}}