{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,19]],"date-time":"2026-03-19T03:28:13Z","timestamp":1773890893368,"version":"3.50.1"},"reference-count":48,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2026,3,1]],"date-time":"2026-03-01T00:00:00Z","timestamp":1772323200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2026,3,1]],"date-time":"2026-03-01T00:00:00Z","timestamp":1772323200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2026,3,1]],"date-time":"2026-03-01T00:00:00Z","timestamp":1772323200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-017"},{"start":{"date-parts":[[2026,3,1]],"date-time":"2026-03-01T00:00:00Z","timestamp":1772323200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2026,3,1]],"date-time":"2026-03-01T00:00:00Z","timestamp":1772323200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-012"},{"start":{"date-parts":[[2026,3,1]],"date-time":"2026-03-01T00:00:00Z","timestamp":1772323200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2026,3,1]],"date-time":"2026-03-01T00:00:00Z","timestamp":1772323200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-004"}],"funder":[{"DOI":"10.13039\/501100019018","name":"Chinese Academy of Medical Sciences Initiative for Innovative Medicine","doi-asserted-by":"publisher","award":["2021-I2M-1\\u2013070"],"award-info":[{"award-number":["2021-I2M-1\\u2013070"]}],"id":[{"id":"10.13039\/501100019018","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Journal of Pharmaceutical and Biomedical Analysis"],"published-print":{"date-parts":[[2026,3]]},"DOI":"10.1016\/j.jpba.2025.117287","type":"journal-article","created":{"date-parts":[[2025,11,28]],"date-time":"2025-11-28T16:40:43Z","timestamp":1764348043000},"page":"117287","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":0,"special_numbering":"C","title":["Economical chiral and achiral separation of seven impurities in brivaracetam by supercritical fluid chromatography with polysaccharide-based chiral stationary phases"],"prefix":"10.1016","volume":"270","author":[{"given":"Qingying","family":"Jia","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaodan","family":"Qiu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xuejia","family":"Zhao","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Huiyi","family":"Liu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yongsheng","family":"Gu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kun","family":"Xiao","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xinran","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0008-5485-8308","authenticated-orcid":false,"given":"Xun","family":"Ma","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0646-2874","authenticated-orcid":false,"given":"Guangzhi","family":"Shan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1016\/j.jpba.2025.117287_bib1","doi-asserted-by":"crossref","first-page":"682","DOI":"10.1038\/s41573-024-00981-w","article-title":"New epilepsy therapies in development","volume":"23","author":"Klein","year":"2024","journal-title":"Nat. Rev. Drug Discov."},{"key":"10.1016\/j.jpba.2025.117287_bib2","doi-asserted-by":"crossref","first-page":"392","DOI":"10.1038\/s41583-022-00604-6","article-title":"Seizures beget seizures","volume":"23","author":"Lewis","year":"2022","journal-title":"Nat. Rev. Neurosci."},{"key":"10.1016\/j.jpba.2025.117287_bib3","doi-asserted-by":"crossref","first-page":"1269","DOI":"10.1001\/jama.2022.3880","article-title":"Antiseizure medications for adults with epilepsy: a review","volume":"327","author":"Kanner","year":"2022","journal-title":"JAMA"},{"key":"10.1016\/j.jpba.2025.117287_bib4","doi-asserted-by":"crossref","first-page":"517","DOI":"10.1007\/s40265-016-0555-6","article-title":"Brivaracetam: first global approval","volume":"76","author":"Markham","year":"2016","journal-title":"Drugs"},{"key":"10.1016\/j.jpba.2025.117287_bib5","series-title":"Brivaracetam [M]","first-page":"6630","author":"EDQM Council of Europe","year":"2025"},{"key":"10.1016\/j.jpba.2025.117287_bib6","doi-asserted-by":"crossref","first-page":"1868","DOI":"10.1039\/C9AY02582K","article-title":"Identification, isolation, structural characterisation, synthesis and in silico toxicity prediction of the alkaline hydrolytic degradation product of brivaracetam by using LC-PDA, preparative HPLC, LC\/HESI\/LTQ, FTIR, and 1H NMR","volume":"12","author":"Bhamare","year":"2020","journal-title":"Anal. Methods"},{"key":"10.1016\/j.jpba.2025.117287_bib7","first-page":"2315","article-title":"Development and validation of stability-indicating UPLC method for the determination of brivaracetam, its related impurities and degradation products","volume":"9","author":"Vishweshwar","year":"2018","journal-title":"Int. J. Pharm. Sci. Res."},{"key":"10.1016\/j.jpba.2025.117287_bib8","doi-asserted-by":"crossref","DOI":"10.1016\/j.tet.2020.131273","article-title":"Identification, characterization, synthesis and strategy for minimization of potential impurities observed in the synthesis of brivaracetam","volume":"76","author":"Liao","year":"2020","journal-title":"Tetrahedron"},{"key":"10.1016\/j.jpba.2025.117287_bib9","article-title":"Current state and future perspectives of supercritical fluid chromatography","volume":"149","author":"Si-Hung","year":"2022","journal-title":"TrACTrends Anal. Chem."},{"key":"10.1016\/j.jpba.2025.117287_bib10","article-title":"Preparative supercritical fluid chromatography as green purification methodology","volume":"171","author":"Peyrin","year":"2024","journal-title":"TrACTrends Anal. Chem."},{"key":"10.1016\/j.jpba.2025.117287_bib11","article-title":"Ultrahigh-performance supercritical fluid chromatography \/ mass spectrometry in the lipidomic analysis","volume":"149","author":"Wolrab","year":"2022","journal-title":"TrACTrends Anal. Chem."},{"key":"10.1016\/j.jpba.2025.117287_bib12","doi-asserted-by":"crossref","first-page":"341","DOI":"10.1016\/j.jpba.2015.06.012","article-title":"Development of a sensitive and rapid method for rifampicin impurity analysis using supercritical fluid chromatography","volume":"114","author":"Li","year":"2015","journal-title":"J. Pharm. Biomed. Anal."},{"key":"10.1016\/j.jpba.2025.117287_bib13","doi-asserted-by":"crossref","DOI":"10.1016\/j.chroma.2025.466257","article-title":"Separation of 15 impurities in semi-synthetic paclitaxel in a single run using supercritical fluid chromatography and greenness assessment by AMGS, GAPI, and AGREE tools","volume":"1759","author":"Zhao","year":"2025","journal-title":"J. Chromatogr. A"},{"key":"10.1016\/j.jpba.2025.117287_bib14","doi-asserted-by":"crossref","first-page":"17551","DOI":"10.1021\/acs.analchem.2c03895","article-title":"Advanced tool for chiral separations of anionic and zwitterionic (Metalla)carboranes: supercritical fluid chromatography","volume":"94","author":"Hor\u00e1\u010dek","year":"2022","journal-title":"Anal. Chem."},{"key":"10.1016\/j.jpba.2025.117287_bib15","doi-asserted-by":"crossref","first-page":"77","DOI":"10.1016\/j.jpba.2012.01.021","article-title":"Supercritical fluid chromatography for the enantioseparation of pharmaceuticals","volume":"69","author":"De Klerck","year":"2012","journal-title":"J. Pharm. Biomed. Anal."},{"key":"10.1016\/j.jpba.2025.117287_bib16","doi-asserted-by":"crossref","first-page":"199","DOI":"10.1016\/j.aca.2016.11.002","article-title":"General screening and optimization strategy for fast chiral separations in modern supercritical fluid chromatography","volume":"950","author":"Nov\u00e1kov\u00e1","year":"2017","journal-title":"Anal. Chim. Acta"},{"key":"10.1016\/j.jpba.2025.117287_bib17","doi-asserted-by":"crossref","first-page":"1789","DOI":"10.1016\/S0731-7085(96)01970-X","article-title":"Comparison of liquid and supercritical fluid chromatography for the separation of enantiomers on chiral stationary phases","volume":"15","author":"Williams","year":"1997","journal-title":"J. Pharm. Biomed. Anal."},{"key":"10.1016\/j.jpba.2025.117287_bib18","doi-asserted-by":"crossref","first-page":"1208","DOI":"10.1002\/chir.23269","article-title":"Development of an effective novel validated stability-indicating HPLC method for the resolution of brivaracetam stereoisomeric impurities","volume":"32","author":"Baksam","year":"2020","journal-title":"Chirality"},{"key":"10.1016\/j.jpba.2025.117287_bib19","first-page":"2084","article-title":"Separation and determination of stereoisomeric impurities in brivaracetam tablets by supercritical fluid chromatography","volume":"59","author":"Zhu","year":"2024","journal-title":"Chin. Pharm. J."},{"key":"10.1016\/j.jpba.2025.117287_bib20","first-page":"57","article-title":"Python: a programming language for software integration and development","volume":"17","author":"Sanner","year":"1999","journal-title":"J. Mol. Graph. Model"},{"key":"10.1016\/j.jpba.2025.117287_bib21","doi-asserted-by":"crossref","first-page":"455","DOI":"10.1002\/jcc.21334","article-title":"AutoDock Vina: improving the speed and accuracy of docking with a new scoring function, efficient optimization, and multithreading","volume":"31","author":"Trott","year":"2010","journal-title":"J. Comput. Chem."},{"key":"10.1016\/j.jpba.2025.117287_bib22","article-title":"Recent trends in chiral supercritical fluid chromatography","volume":"120","author":"West","year":"2019","journal-title":"TrACTrends Anal. Chem."},{"key":"10.1016\/j.jpba.2025.117287_bib23","doi-asserted-by":"crossref","first-page":"232","DOI":"10.1016\/j.jpba.2015.12.007","article-title":"Chiral separations of cathinone and amphetamine-derivatives: comparative study between capillary electrochromatography, supercritical fluid chromatography and three liquid chromatographic modes","volume":"121","author":"Albals","year":"2016","journal-title":"J. Pharm. Biomed. Anal."},{"key":"10.1016\/j.jpba.2025.117287_bib24","doi-asserted-by":"crossref","DOI":"10.1002\/chir.23626","article-title":"Investigation into the performance and stability of immobilized and coated polysaccharide columns in supercritical fluid chromatography","volume":"36","author":"Yue","year":"2024","journal-title":"Chirality"},{"key":"10.1016\/j.jpba.2025.117287_bib25","doi-asserted-by":"crossref","first-page":"294","DOI":"10.1016\/j.chroma.2014.06.026","article-title":"Insights into chiral recognition mechanism in supercritical fluid chromatography IV. Chlorinated polysaccharide stationary phases","volume":"1363","author":"Khater","year":"2014","journal-title":"J. Chromatogr. A"},{"key":"10.1016\/j.jpba.2025.117287_bib26","doi-asserted-by":"crossref","first-page":"9","DOI":"10.3390\/separations10010009","article-title":"Study of different chiral columns for the enantiomeric separation of azoles using supercritical fluid chromatography","volume":"10","author":"Toribio","year":"2023","journal-title":"Separations"},{"key":"10.1016\/j.jpba.2025.117287_bib27","doi-asserted-by":"crossref","first-page":"89","DOI":"10.1016\/j.chroma.2017.06.049","article-title":"The effect of high concentration additive on chiral separations in supercritical fluid chromatography","volume":"1510","author":"Speybrouck","year":"2017","journal-title":"J. Chromatogr. A"},{"key":"10.1016\/j.jpba.2025.117287_bib28","doi-asserted-by":"crossref","DOI":"10.1016\/j.chroma.2021.462270","article-title":"Supercritical fluid chromatography and liquid chromatography for isomeric separation of a multiple chiral centers analyte","volume":"1651","author":"La","year":"2021","journal-title":"J. Chromatogr. A"},{"key":"10.1016\/j.jpba.2025.117287_bib29","doi-asserted-by":"crossref","first-page":"3947","DOI":"10.1021\/jp003846y","article-title":"In situ formation of alkylcarbonic acids with CO2","volume":"105","author":"West","year":"2001","journal-title":"J. Phys. Chem. A"},{"key":"10.1016\/j.jpba.2025.117287_bib30","doi-asserted-by":"crossref","first-page":"136","DOI":"10.1016\/j.chroma.2017.02.066","article-title":"Unravelling the effects of mobile phase additives in supercritical fluid chromatography. Part I: Polarity and acidity of the mobile phase","volume":"1492","author":"West","year":"2017","journal-title":"J. Chromatogr. A"},{"key":"10.1016\/j.jpba.2025.117287_bib31","doi-asserted-by":"crossref","first-page":"2124","DOI":"10.3390\/molecules29092124","article-title":"The impact of water as an additive on the elution of some basic organic compounds in supercritical fluid chromatography","volume":"29","author":"Kazmouz","year":"2024","journal-title":"Molecules"},{"key":"10.1016\/j.jpba.2025.117287_bib32","doi-asserted-by":"crossref","first-page":"135","DOI":"10.1016\/j.chroma.2019.02.002","article-title":"Unravelling the effects of mobile phase additives in supercritical fluid chromatography\u2014Part II: Adsorption on the stationary phase","volume":"1593","author":"West","year":"2019","journal-title":"J. Chromatogr. A"},{"key":"10.1016\/j.jpba.2025.117287_bib33","doi-asserted-by":"crossref","first-page":"4569","DOI":"10.1016\/j.chroma.2011.05.038","article-title":"Use of isopycnic plots in designing operations of supercritical fluid chromatography: I. The critical role of density in determining the characteristics of the mobile phase in supercritical fluid chromatography","volume":"1218","author":"Tarafder","year":"2011","journal-title":"J. Chromatogr. A"},{"key":"10.1016\/j.jpba.2025.117287_bib34","doi-asserted-by":"crossref","DOI":"10.1016\/j.chroma.2019.460600","article-title":"Evaluation of temperature and pressure effects on retention in supercritical fluid chromatography on polar stationary phases","volume":"1610","author":"Ovchinnikov","year":"2020","journal-title":"J. Chromatogr. A"},{"key":"10.1016\/j.jpba.2025.117287_bib35","doi-asserted-by":"crossref","DOI":"10.1016\/j.chroma.2024.465342","article-title":"Economical and rapid enantioselective, diastereoselective and achiral separation of palonosetron hydrochloride and its impurities using supercritical fluid chromatography","volume":"1736","author":"Qiu","year":"2024","journal-title":"J. Chromatogr. A"},{"key":"10.1016\/j.jpba.2025.117287_bib36","doi-asserted-by":"crossref","first-page":"65","DOI":"10.1016\/j.jpba.2017.08.021","article-title":"Supercritical fluid chromatography in traditional Chinese medicine analysis","volume":"147","author":"Huang","year":"2018","journal-title":"J. Pharm. Biomed. Anal."},{"key":"10.1016\/j.jpba.2025.117287_bib37","doi-asserted-by":"crossref","first-page":"57","DOI":"10.1016\/S0021-9673(97)00693-6","article-title":"Temperature and pressure effects on solubility in supercritical carbon dioxide and retention in supercritical fluid chromatography","volume":"785","author":"Lou","year":"1997","journal-title":"J. Chromatogr. A"},{"key":"10.1016\/j.jpba.2025.117287_bib38","doi-asserted-by":"crossref","first-page":"325","DOI":"10.1016\/j.chroma.2012.09.078","article-title":"Effects of mobile phase composition and temperature on the supercritical fluid chromatography enantioseparation of chiral fluoro-oxoindole-type compounds with chlorinated polysaccharide stationary phases","volume":"1269","author":"West","year":"2012","journal-title":"J. Chromatogr. A"},{"key":"10.1016\/j.jpba.2025.117287_bib39","doi-asserted-by":"crossref","DOI":"10.1016\/j.chroma.2024.465250","article-title":"On physical meaning of van't Hoff equation and its applicability in chromatography","volume":"1733","author":"Asnin","year":"2024","journal-title":"J. Chromatogr. A"},{"key":"10.1016\/j.jpba.2025.117287_bib40","doi-asserted-by":"crossref","DOI":"10.1016\/j.chroma.2020.461299","article-title":"Analytical separation of four stereoisomers of luliconazole using supercritical fluid chromatography: Thermodynamic aspects and simulation study with chiral stationary phase","volume":"1625","author":"Pandya","year":"2020","journal-title":"J. Chromatogr. A"},{"key":"10.1016\/j.jpba.2025.117287_bib41","doi-asserted-by":"crossref","first-page":"152","DOI":"10.1002\/bmc.3013","article-title":"Effects of organic modifier and temperature on the enantiomeric separation of several azole drugs using supercritical fluid chromatography and the Chiralpak AD column","volume":"28","author":"Toribio","year":"2014","journal-title":"Biomed. Chromatogr."},{"key":"10.1016\/j.jpba.2025.117287_bib42","doi-asserted-by":"crossref","first-page":"2179","DOI":"10.1021\/ac960029e","article-title":"Entropically Driven\u201d chiral separations in supercritical fluid chromatography. Confirmation of isoelution temperature and reversal of elution order","volume":"68","author":"Stringham","year":"1996","journal-title":"Anal. Chem."},{"key":"10.1016\/j.jpba.2025.117287_bib43","doi-asserted-by":"crossref","DOI":"10.1016\/j.jpba.2020.113452","article-title":"Separation of achiral anti-diabetic drugs using sub\/supercritical fluid chromatography with a polysaccharide stationary phase: Thermodynamic considerations and molecular docking study","volume":"189","author":"Pandya","year":"2020","journal-title":"J. Pharm. Biomed. Anal."},{"key":"10.1016\/j.jpba.2025.117287_bib44","doi-asserted-by":"crossref","DOI":"10.1016\/j.chroma.2025.466220","article-title":"Exploration of the enantiorecognition mechanism of protected amino acids on amylose tris (3,5-dimethylphenylcarbamate) stationary phase in supercritical fluid chromatography: Thermodynamic considerations and molecular docking study","volume":"1758","author":"Qiu","year":"2025","journal-title":"J. Chromatogr. A"},{"key":"10.1016\/j.jpba.2025.117287_bib45","doi-asserted-by":"crossref","DOI":"10.1016\/j.jpba.2021.114458","article-title":"Separation of chiral and achiral impurities in paroxetine hydrochloride in a single run using supercritical fluid chromatography with a polysaccharide stationary phase","volume":"208","author":"Qiu","year":"2022","journal-title":"J. Pharm. Biomed. Anal."},{"key":"10.1016\/j.jpba.2025.117287_bib46","unstructured":"International Council for Harmonization, Guideline Q2 (R1) Validation of analytical procedures: Text and methodology [G], Geneva: ICH, 2005."},{"key":"10.1016\/j.jpba.2025.117287_bib47","article-title":"White analytical chemistry: an approach to reconcile the principles of green analytical chemistry and functionality","volume":"138","author":"Nowak","year":"2021","journal-title":"TrACTrends Anal. Chem."},{"key":"10.1016\/j.jpba.2025.117287_bib48","doi-asserted-by":"crossref","first-page":"1816","DOI":"10.1039\/C8GC03875A","article-title":"Making the move towards modernized greener separations: introduction of the analytical method greenness score (AMGS) calculator","volume":"21","author":"Hicks","year":"2019","journal-title":"Green. Chem."}],"container-title":["Journal of Pharmaceutical and Biomedical Analysis"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0731708525006284?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0731708525006284?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,3,19]],"date-time":"2026-03-19T00:24:20Z","timestamp":1773879860000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0731708525006284"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,3]]},"references-count":48,"alternative-id":["S0731708525006284"],"URL":"https:\/\/doi.org\/10.1016\/j.jpba.2025.117287","relation":{},"ISSN":["0731-7085"],"issn-type":[{"value":"0731-7085","type":"print"}],"subject":[],"published":{"date-parts":[[2026,3]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Economical chiral and achiral separation of seven impurities in brivaracetam by supercritical fluid chromatography with polysaccharide-based chiral stationary phases","name":"articletitle","label":"Article Title"},{"value":"Journal of Pharmaceutical and Biomedical Analysis","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.jpba.2025.117287","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2025 Published by Elsevier B.V.","name":"copyright","label":"Copyright"}],"article-number":"117287"}}