{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,4]],"date-time":"2026-09-04T14:54:40Z","timestamp":1788533680963,"version":"build-2803163510"},"reference-count":26,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2026,8,1]],"date-time":"2026-08-01T00:00:00Z","timestamp":1785542400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2026,8,1]],"date-time":"2026-08-01T00:00:00Z","timestamp":1785542400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2026,8,1]],"date-time":"2026-08-01T00:00:00Z","timestamp":1785542400000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-017"},{"start":{"date-parts":[[2026,8,1]],"date-time":"2026-08-01T00:00:00Z","timestamp":1785542400000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2026,8,1]],"date-time":"2026-08-01T00:00:00Z","timestamp":1785542400000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-012"},{"start":{"date-parts":[[2026,8,1]],"date-time":"2026-08-01T00:00:00Z","timestamp":1785542400000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2026,8,1]],"date-time":"2026-08-01T00:00:00Z","timestamp":1785542400000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-004"}],"funder":[{"DOI":"10.13039\/501100005758","name":"Universit\u00e0 Politecnica delle Marche","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100005758","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004801","name":"LEPL Shota Rustaveli National Science Foundation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004801","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Journal of Pharmaceutical and Biomedical Analysis"],"published-print":{"date-parts":[[2026,8]]},"DOI":"10.1016\/j.jpba.2026.117474","type":"journal-article","created":{"date-parts":[[2026,3,24]],"date-time":"2026-03-24T07:52:06Z","timestamp":1774338726000},"page":"117474","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":5,"special_numbering":"C","title":["First enantioselective determination of N-ethylpentedrone and its major phase-1 metabolites using supercritical fluid chromatography coupled with tandem mass spectrometry and its application to human oral fluid and urine samples"],"prefix":"10.1016","volume":"276","author":[{"ORCID":"https:\/\/orcid.org\/0009-0007-4751-6396","authenticated-orcid":false,"given":"Saba","family":"Jorbenadze","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aluda","family":"Tchelidze","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Giorgia","family":"Sprega","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vazha","family":"Tkemaladze","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gizo","family":"Dolidze","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8137-0614","authenticated-orcid":false,"given":"Adele","family":"Minutillo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Magi","family":"Farr\u00e9","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lourdes","family":"Poyatos","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Esther","family":"Papaseit","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Giuseppe","family":"Basile","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tivadar","family":"Farkas","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Francesco Paolo","family":"Busardo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2379-9815","authenticated-orcid":false,"given":"Bezhan","family":"Chankvetadze","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"issue":"12","key":"10.1016\/j.jpba.2026.117474_bib1","doi-asserted-by":"crossref","first-page":"153","DOI":"10.1021\/ac00036a009","article-title":"Supercritical fluid chromatography and extraction","volume":"64","author":"Chester","year":"1992","journal-title":"Anal. Chem."},{"key":"10.1016\/j.jpba.2026.117474_bib2","doi-asserted-by":"crossref","first-page":"141","DOI":"10.1016\/j.chroma.2016.04.040","article-title":"Analysis of polar urinary metabolites for metabolic phenotyping using supercritical fluid chromatography and mass spectrometry","volume":"1449","author":"Sen","year":"2016","journal-title":"J. Chromatogr. A"},{"key":"10.1016\/j.jpba.2026.117474_bib3","doi-asserted-by":"crossref","first-page":"96","DOI":"10.1016\/j.chroma.2018.05.055","article-title":"Applicability of supercritical fluid chromatography\u2013mass spectrometry to metabolomics. I \u2013 Optimization of separation conditions for the simultaneous analysis of hydrophilic and lipophilic substances","volume":"1562","author":"Desfontaine","year":"2018","journal-title":"J. Chromatogr. A"},{"key":"10.1016\/j.jpba.2026.117474_bib4","doi-asserted-by":"crossref","DOI":"10.1016\/j.chroma.2020.461587","article-title":"Development of a novel method for polar metabolite profiling by supercritical fluid chromatography\u2013tandem mass spectrometry","volume":"1632","author":"Konya","year":"2020","journal-title":"J. Chromatogr. A"},{"key":"10.1016\/j.jpba.2026.117474_bib5","doi-asserted-by":"crossref","DOI":"10.1016\/j.chroma.2019.460449","article-title":"Supercritical fluid chromatography\u2013mass spectrometry using data independent acquisition for the analysis of polar metabolites in human urine","volume":"1609","author":"Akbal","year":"2020","journal-title":"J. Chromatogr. A"},{"key":"10.1016\/j.jpba.2026.117474_bib6","doi-asserted-by":"crossref","DOI":"10.1016\/j.trac.2021.116304","article-title":"Metamorphosis of supercritical fluid chromatography: a viable tool for the analysis of polar compounds?","volume":"141","author":"Losacco","year":"2021","journal-title":"TrAC Trends Anal. Chem."},{"key":"10.1016\/j.jpba.2026.117474_bib7","article-title":"Current state and future perspectives of supercritical fluid chromatography","volume":"149","author":"Lee","year":"2022","journal-title":"TrAC Trends Anal. Chem."},{"key":"10.1016\/j.jpba.2026.117474_bib8","doi-asserted-by":"crossref","DOI":"10.1002\/jssc.70003","article-title":"Supercritical fluid chromatography in bioanalysis \u2013 A review","volume":"47","author":"Toribio","year":"2024","journal-title":"J. Sep. Sci."},{"key":"10.1016\/j.jpba.2026.117474_bib9","doi-asserted-by":"crossref","first-page":"212","DOI":"10.1016\/j.trac.2018.12.023","article-title":"Recent developments in supercritical fluid chromatography\u2013mass spectrometry: is it a viable option for analysis of complex samples?","volume":"112","author":"Pila\u0159ov\u00e1","year":"2019","journal-title":"TrAC Trends Anal. Chem."},{"issue":"1\u20132","key":"10.1016\/j.jpba.2026.117474_bib10","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.aca.2011.02.047","article-title":"Stationary and mobile phases in hydrophilic interaction chromatography: a review","volume":"692","author":"Jandera","year":"2011","journal-title":"Anal. Chim. Acta"},{"key":"10.1016\/j.jpba.2026.117474_bib11","doi-asserted-by":"crossref","DOI":"10.3389\/fphar.2023.1145140","article-title":"Metabolic stability and metabolite profiling of emerging synthetic cathinones","volume":"14","author":"Lopes","year":"2023","journal-title":"Front. Pharm."},{"key":"10.1016\/j.jpba.2026.117474_bib12","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.jchromb.2016.12.018","article-title":"Identification of novel psychoactive substances 25B-NBOMe and 4-CMC in biological material using HPLC-Q-TOF-MS and their quantification in blood using UPLC\u2013MS\/MS in case of severe intoxications","volume":"1041\u20131042","author":"Wiergowski","year":"2017","journal-title":"J. Chromatogr. B"},{"issue":"5","key":"10.1016\/j.jpba.2026.117474_bib13","doi-asserted-by":"crossref","first-page":"233","DOI":"10.1093\/jat\/bku021","article-title":"Testing for designer stimulants: metabolic profiles of 16 synthetic cathinones excreted free in human urine","volume":"38","author":"Uralets","year":"2014","journal-title":"J. Anal. Toxicol."},{"key":"10.1016\/j.jpba.2026.117474_bib14","doi-asserted-by":"crossref","first-page":"25","DOI":"10.1007\/s11419-022-00639-5","article-title":"A review of synthetic cathinones emerging in recent years (2019\u20132022)","volume":"41","author":"Kuropka","year":"2023","journal-title":"Forensic Toxicol."},{"key":"10.1016\/j.jpba.2026.117474_bib15","doi-asserted-by":"crossref","DOI":"10.1016\/j.jpba.2024.116293","article-title":"Simultaneous chemo- and enantio-separation of 2-, 3- and 4-chloro-methcathinones by high-performance liquid chromatography\u2013tandem mass spectrometry and its application to oral fluid samples","volume":"248","author":"Kobidze","year":"2024","journal-title":"J. Pharm. Biomed. Anal."},{"key":"10.1016\/j.jpba.2026.117474_bib16","doi-asserted-by":"crossref","DOI":"10.1007\/s00216-025-05838-8","article-title":"Simultaneous enantioselective determination of 2-, 3- and 4-methylmethcathinones, their isomers and major phase-I metabolites in oral fluid of drug abusers using enantioselective high-performance liquid chromatography\u2013tandem mass spectrometry","volume":"417","author":"Kobidze","year":"2025","journal-title":"Anal. Bioanal. Chem."},{"key":"10.1016\/j.jpba.2026.117474_bib17","doi-asserted-by":"crossref","DOI":"10.1016\/j.jpba.2024.116350","article-title":"Investigation of stereochemical stability of 2-, 3- and 4-chloromethcathinones in various biological matrices","volume":"249","author":"Jorbenadze","year":"2024","journal-title":"J. Pharm. Biomed. Anal."},{"key":"10.1016\/j.jpba.2026.117474_bib18","doi-asserted-by":"crossref","DOI":"10.1016\/j.jpba.2024.116275","article-title":"Challenges encountered in the enantioselective analysis of new psychoactive substances exemplified by clephedrone (4-CMC)","volume":"248","author":"Jorbenadze","year":"2024","journal-title":"J. Pharm. Biomed. Anal."},{"key":"10.1016\/j.jpba.2026.117474_bib19","doi-asserted-by":"crossref","DOI":"10.1016\/j.jpba.2026.117375","article-title":"Enantioselective high-performance liquid chromatography\u2013tandem mass spectrometry method for analysis of new psychoactive substance N-ethylpentedrone","volume":"272","author":"Jorbenadze","year":"2026","journal-title":"J. Pharm. Biomed. Anal."},{"issue":"25","key":"10.1016\/j.jpba.2026.117474_bib20","doi-asserted-by":"crossref","first-page":"6441","DOI":"10.1007\/s00216-018-1267-4","article-title":"Current trends in supercritical fluid chromatography","volume":"410","author":"West","year":"2018","journal-title":"Anal. Bioanal. Chem."},{"key":"10.1016\/j.jpba.2026.117474_bib21","doi-asserted-by":"crossref","DOI":"10.1016\/j.trac.2019.115648","article-title":"Recent trends in chiral supercritical fluid chromatography","volume":"120","author":"West","year":"2019","journal-title":"TrAC Trends Anal. Chem."},{"key":"10.1016\/j.jpba.2026.117474_bib22","doi-asserted-by":"crossref","DOI":"10.1016\/j.trac.2024.117733","article-title":"Application of enantioseparation techniques to forensic analysis and clinical toxicology","volume":"175","author":"Busardo","year":"2024","journal-title":"TrAC Trends Anal. Chem."},{"key":"10.1016\/j.jpba.2026.117474_bib23","doi-asserted-by":"crossref","DOI":"10.1016\/j.jpba.2024.115994","article-title":"Metabolic profile of N-ethylhexedrone, N-ethylpentedrone, and 4-chloro-methcathinone in urine samples by UHPLC-QTOF-HRMS","volume":"241","author":"Massano","year":"2024","journal-title":"J. Pharm. Biomed. Anal."},{"key":"10.1016\/j.jpba.2026.117474_bib24","doi-asserted-by":"crossref","DOI":"10.1016\/j.jpba.2025.117170","article-title":"Comparison of authentic urine N-ethylpentedrone metabolites to predicted in silico and in vitro human hepatocyte metabolism","volume":"267","author":"Daziani","year":"2026","journal-title":"J. Pharm. Biomed. Anal."},{"key":"10.1016\/j.jpba.2026.117474_bib25","doi-asserted-by":"crossref","first-page":"257","DOI":"10.3390\/pharmaceutics16020257","article-title":"Metabolic stability and metabolite identification of N-ethylpentedrone using rat, mouse and human liver microsomes","volume":"16","author":"Barcia Godoi","year":"2024","journal-title":"Pharmaceutics"},{"key":"10.1016\/j.jpba.2026.117474_bib26","unstructured":"European Monitoring Centre for Drugs and Drug Addiction, European Drug Report 2025: New psychoactive substances, 2025."}],"container-title":["Journal of Pharmaceutical and Biomedical Analysis"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0731708526001421?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0731708526001421?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,4,14]],"date-time":"2026-04-14T03:40:45Z","timestamp":1776138045000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0731708526001421"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,8]]},"references-count":26,"alternative-id":["S0731708526001421"],"URL":"https:\/\/doi.org\/10.1016\/j.jpba.2026.117474","relation":{},"ISSN":["0731-7085"],"issn-type":[{"value":"0731-7085","type":"print"}],"subject":[],"published":{"date-parts":[[2026,8]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"First enantioselective determination of N-ethylpentedrone and its major phase-1 metabolites using supercritical fluid chromatography coupled with tandem mass spectrometry and its application to human oral fluid and urine samples","name":"articletitle","label":"Article Title"},{"value":"Journal of Pharmaceutical and Biomedical Analysis","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.jpba.2026.117474","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2026 Elsevier B.V. All rights are reserved, including those for text and data mining, AI training, and similar technologies.","name":"copyright","label":"Copyright"}],"article-number":"117474"}}