{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,25]],"date-time":"2026-04-25T06:49:33Z","timestamp":1777099773758,"version":"3.51.4"},"reference-count":30,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T00:00:00Z","timestamp":1788220800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T00:00:00Z","timestamp":1788220800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T00:00:00Z","timestamp":1788220800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-017"},{"start":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T00:00:00Z","timestamp":1788220800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T00:00:00Z","timestamp":1788220800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-012"},{"start":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T00:00:00Z","timestamp":1788220800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T00:00:00Z","timestamp":1788220800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-004"}],"funder":[{"DOI":"10.13039\/501100008549","name":"Shanxi Medical University","doi-asserted-by":"publisher","award":["CKD\/SXMU-2025-06"],"award-info":[{"award-number":["CKD\/SXMU-2025-06"]}],"id":[{"id":"10.13039\/501100008549","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100005891","name":"State Administration of Traditional Chinese Medicine of the People's Republic of China","doi-asserted-by":"publisher","award":["GZY-KJS-2023-031"],"award-info":[{"award-number":["GZY-KJS-2023-031"]}],"id":[{"id":"10.13039\/501100005891","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81973287"],"award-info":[{"award-number":["81973287"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Journal of Pharmaceutical and Biomedical Analysis"],"published-print":{"date-parts":[[2026,9]]},"DOI":"10.1016\/j.jpba.2026.117497","type":"journal-article","created":{"date-parts":[[2026,4,3]],"date-time":"2026-04-03T06:15:56Z","timestamp":1775196956000},"page":"117497","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":0,"special_numbering":"C","title":["Discovery of anti-renal fibrosis quality markers from Piperis longi fructus: An integrated strategy of hollow fibre cell trapping method coupled with liquid chromatography-mass spectrometry and in vitro efficacy verification"],"prefix":"10.1016","volume":"277","author":[{"given":"Xue-ling","family":"Zhang","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qian","family":"Li","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Run-qin","family":"Wang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rong-rong","family":"Xing","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xuan","family":"Chen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jian-fang","family":"Liu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shuang","family":"Hu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1016\/j.jpba.2026.117497_bib1","doi-asserted-by":"crossref","first-page":"129","DOI":"10.1038\/s41392-023-01379-7","article-title":"Kidney fibrosis: from mechanisms to therapeutic medicines","volume":"8","author":"Huang","year":"2023","journal-title":"Signal Transduct. Target. Ther."},{"key":"10.1016\/j.jpba.2026.117497_bib2","doi-asserted-by":"crossref","first-page":"816","DOI":"10.1038\/s41419-023-06347-1","article-title":"Inhibition of PFKP in renal tubular epithelial cell restrains TGF-\u03b2 induced glycolysis and renal fibrosis","volume":"14","author":"Yang","year":"2023","journal-title":"Cell Death Dis."},{"key":"10.1016\/j.jpba.2026.117497_bib3","doi-asserted-by":"crossref","first-page":"325","DOI":"10.1038\/nrneph.2016.48","article-title":"TGF-\u03b2: the master regulator of fibrosis","volume":"12","author":"Meng","year":"2016","journal-title":"Nat. Rev. Nephrol."},{"key":"10.1016\/j.jpba.2026.117497_bib4","doi-asserted-by":"crossref","first-page":"295","DOI":"10.1016\/j.addr.2017.12.019","article-title":"Targeting renal fibrosis: mechanisms and drug delivery systems","volume":"129","author":"Nastase","year":"2018","journal-title":"Adv. Drug Deliv. Rev."},{"key":"10.1016\/j.jpba.2026.117497_bib5","doi-asserted-by":"crossref","DOI":"10.1016\/j.phymed.2021.153650","article-title":"Effects of andrographolide on renal tubulointersticial injury and fibrosis. Evidence of its mechanism of action","volume":"91","author":"Liu","year":"2021","journal-title":"Phytomedicine"},{"key":"10.1016\/j.jpba.2026.117497_bib6","doi-asserted-by":"crossref","first-page":"10682","DOI":"10.1038\/s41598-020-67690-3","article-title":"Transcriptomic analysis of the mechanisms of alleviating renal interstitial fibrosis using the traditional Chinese medicine Kangxianling in a rat model","volume":"10","author":"Jiang","year":"2020","journal-title":"Sci. Rep."},{"key":"10.1016\/j.jpba.2026.117497_bib7","article-title":"Research progress of Chinese herbal medicine intervention in renal interstitial fibrosis","volume":"13","author":"Liu","year":"2022","journal-title":"Front. Pharm."},{"key":"10.1016\/j.jpba.2026.117497_bib8","doi-asserted-by":"crossref","first-page":"4425","DOI":"10.1002\/ptr.7649","article-title":"A comprehensive review on traditional uses, phytochemistry, pharmacology, and health-promoting activities","volume":"36","author":"Biswas","year":"2022","journal-title":"Phytother. Res."},{"key":"10.1016\/j.jpba.2026.117497_bib9","doi-asserted-by":"crossref","DOI":"10.1016\/j.jep.2019.112255","article-title":"A systematic review on Piper longum L.: Bridging traditional knowledge and pharmacological evidence for future translational research","volume":"247","author":"Yadav","year":"2020","journal-title":"J. Ethnopharmacol."},{"key":"10.1016\/j.jpba.2026.117497_bib10","doi-asserted-by":"crossref","first-page":"373","DOI":"10.1016\/j.jep.2013.03.022","article-title":"Anti-snake venom activities of ethanolic extract of fruits of Piper longum L.(Piperaceae) against Russell's viper venom: characterization of piperine as active principle","volume":"147","author":"Shenoy","year":"2013","journal-title":"J. Ethnopharmacol."},{"key":"10.1016\/j.jpba.2026.117497_bib11","doi-asserted-by":"crossref","DOI":"10.1016\/j.jep.2023.116561","article-title":"Piperlonguminine attenuates renal fibrosis by inhibiting TRPC6","volume":"313","author":"Zhou","year":"2023","journal-title":"J. Ethnopharmacol."},{"key":"10.1016\/j.jpba.2026.117497_bib12","series-title":"Pharmacopoeia of the People\u2019s Republic of China, Part I","first-page":"254","author":"National Pharmacopoeia Commission","year":"2025"},{"key":"10.1016\/j.jpba.2026.117497_bib13","doi-asserted-by":"crossref","first-page":"680","DOI":"10.1002\/ptr.6855","article-title":"Piperine: a review of its biological effects","volume":"35","author":"Haq","year":"2021","journal-title":"Phytother. Res."},{"key":"10.1016\/j.jpba.2026.117497_bib14","doi-asserted-by":"crossref","DOI":"10.1016\/j.trac.2022.116601","article-title":"Recent advances in cell membrane-coated technology for drug discovery from natural products","volume":"151","author":"Bu","year":"2022","journal-title":"TrAC-Trends Anal. Chem."},{"key":"10.1016\/j.jpba.2026.117497_bib15","doi-asserted-by":"crossref","DOI":"10.1016\/j.jpba.2020.113825","article-title":"A stop-flow comprehensive two-dimensional HK-2 and HK-2\/CIKI cell membrane chromatography comparative analysis system for screening the active ingredients from Pyrrosia calvata (Bak.) Ching against crystal-induced kidney injury","volume":"195","author":"Pan","year":"2021","journal-title":"J. Pharm. Biomed. Anal."},{"key":"10.1016\/j.jpba.2026.117497_bib16","doi-asserted-by":"crossref","DOI":"10.1016\/j.jep.2020.112687","article-title":"Compound-based Chinese medicine formula: from discovery to compatibility mechanism","volume":"254","author":"Luan","year":"2020","journal-title":"J. Ethnopharmacol."},{"key":"10.1016\/j.jpba.2026.117497_bib17","doi-asserted-by":"crossref","first-page":"269","DOI":"10.1016\/j.cej.2019.01.171","article-title":"Magnetic carbon nanotubes camouflaged with cell membrane as a drug discovery platform for selective extraction of bioactive compounds from natural products","volume":"364","author":"Hu","year":"2019","journal-title":"Chem. Eng. J."},{"key":"10.1016\/j.jpba.2026.117497_bib18","doi-asserted-by":"crossref","first-page":"8","DOI":"10.1016\/j.chroma.2013.10.084","article-title":"Hollow fibre cell fishing with high performance liquid chromatography for screening bioactive anthraquinones from traditional Chinese medicines","volume":"1322","author":"Yan","year":"2013","journal-title":"J. Chromatogr. A"},{"key":"10.1016\/j.jpba.2026.117497_bib19","doi-asserted-by":"crossref","first-page":"118","DOI":"10.1016\/j.jchromb.2017.06.003","article-title":"Study on major antitumor components in Yinchenhao decoction in vitro and in vivo based on hollow fiber cell fishing coupled with high performance liquid chromatography","volume":"1060","author":"Wu","year":"2017","journal-title":"J. Chromatogr. B"},{"key":"10.1016\/j.jpba.2026.117497_bib20","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1016\/j.jpba.2017.10.026","article-title":"Research on major antitumor active components in Zi-Cao-Cheng-Qi decoction based on hollow fiber cell fishing with high performance liquid chromatography","volume":"149","author":"Li","year":"2018","journal-title":"J. Pharm. Biomed. Anal."},{"key":"10.1016\/j.jpba.2026.117497_bib21","doi-asserted-by":"crossref","first-page":"69","DOI":"10.1007\/s00418-023-02237-x","article-title":"HK\u20112 cell response to TGF\u2011\u03b2 highly depends on cell culture medium formulations","volume":"161","author":"Garmaa","year":"2024","journal-title":"Histochem. Cell Biol."},{"key":"10.1016\/j.jpba.2026.117497_bib22","doi-asserted-by":"crossref","first-page":"28","DOI":"10.1016\/j.jpba.2015.02.012","article-title":"Qualitative and quantitative analysis of an alkaloid fraction from Piper longum L. using ultra-high performance liquid chromatography-diode array detector\u2013electrospray ionization mass spectrometry","volume":"109","author":"Li","year":"2015","journal-title":"J. Pharm. Biomed. Anal."},{"key":"10.1016\/j.jpba.2026.117497_bib23","doi-asserted-by":"crossref","first-page":"191","DOI":"10.1016\/j.foodchem.2015.01.033","article-title":"Identification and simultaneous quantification of five alkaloids in Piper longum L. by HPLC\u2013ESI-MSn and UFLC\u2013ESI-MS\/MS and their application to Piper nigrum L","volume":"177","author":"Liu","year":"2015","journal-title":"Food Chem."},{"key":"10.1016\/j.jpba.2026.117497_bib24","doi-asserted-by":"crossref","DOI":"10.1016\/j.foodres.2021.110123","article-title":"LC-HRMS\/MS-based phytochemical profiling of Piper spices: global association of piperamides with endocannabinoid system modulation","volume":"141","author":"Luca","year":"2021","journal-title":"Food Res. Int."},{"key":"10.1016\/j.jpba.2026.117497_bib25","doi-asserted-by":"crossref","first-page":"679","DOI":"10.1007\/s12272-001-1212-7","article-title":"Methylpiperate derivatives from Piper longum and their inhibition of monoamine oxidase","volume":"31","author":"Lee","year":"2008","journal-title":"Arch. Pharm. Res."},{"key":"10.1016\/j.jpba.2026.117497_bib26","first-page":"110","article-title":"Cell membrance chromatography and its application in researching active components of Chinese materia medica","volume":"34","author":"Wang","year":"2011","journal-title":"Drug Eval. Res."},{"key":"10.1016\/j.jpba.2026.117497_bib27","doi-asserted-by":"crossref","first-page":"439","DOI":"10.1016\/j.apsb.2017.04.012","article-title":"Approaches to establish Q-markers for the quality standards of traditional Chinese medicines","volume":"7","author":"Yang","year":"2017","journal-title":"Acta Pharm. Sin. B"},{"key":"10.1016\/j.jpba.2026.117497_bib28","first-page":"1994","article-title":"Piperine regulates pyroptosis of fibroblast synoviocytes in rheumatoid arthritis by secreted frizzled related protein","volume":"41","author":"Zhang","year":"2025","journal-title":"Chin. J. Clin. Pharm."},{"key":"10.1016\/j.jpba.2026.117497_bib29","doi-asserted-by":"crossref","DOI":"10.1016\/j.jep.2023.116561","article-title":"Piperlonguminine attenuates renal fibrosis by inhibiting TRPC6","volume":"313","author":"Zhou","year":"2023","journal-title":"J. Ethnopharmacol."},{"key":"10.1016\/j.jpba.2026.117497_bib30","doi-asserted-by":"crossref","DOI":"10.1016\/j.fct.2023.114051","article-title":"Piperlongumine induces apoptosis and autophagy via the PI3K\/Akt\/mTOR pathway in KB human cervical cancer cells","volume":"180","author":"Han","year":"2023","journal-title":"Food Chem. Toxicol."}],"container-title":["Journal of Pharmaceutical and Biomedical Analysis"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0731708526001652?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0731708526001652?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,4,25]],"date-time":"2026-04-25T06:15:59Z","timestamp":1777097759000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0731708526001652"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,9]]},"references-count":30,"alternative-id":["S0731708526001652"],"URL":"https:\/\/doi.org\/10.1016\/j.jpba.2026.117497","relation":{},"ISSN":["0731-7085"],"issn-type":[{"value":"0731-7085","type":"print"}],"subject":[],"published":{"date-parts":[[2026,9]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Discovery of anti-renal fibrosis quality markers from Piperis longi fructus: An integrated strategy of hollow fibre cell trapping method coupled with liquid chromatography-mass spectrometry and in vitro efficacy verification","name":"articletitle","label":"Article Title"},{"value":"Journal of Pharmaceutical and Biomedical Analysis","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.jpba.2026.117497","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2026 Elsevier B.V. All rights are reserved, including those for text and data mining, AI training, and similar technologies.","name":"copyright","label":"Copyright"}],"article-number":"117497"}}