{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,3]],"date-time":"2026-07-03T03:17:34Z","timestamp":1783048654044,"version":"3.54.6"},"reference-count":36,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T00:00:00Z","timestamp":1788220800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T00:00:00Z","timestamp":1788220800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T00:00:00Z","timestamp":1788220800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-017"},{"start":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T00:00:00Z","timestamp":1788220800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T00:00:00Z","timestamp":1788220800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-012"},{"start":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T00:00:00Z","timestamp":1788220800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T00:00:00Z","timestamp":1788220800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-004"}],"funder":[{"DOI":"10.13039\/501100005891","name":"State Administration of Traditional Chinese Medicine of the People's Republic of China","doi-asserted-by":"publisher","award":["5001\\u201312263004"],"award-info":[{"award-number":["5001\\u201312263004"]}],"id":[{"id":"10.13039\/501100005891","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82260755"],"award-info":[{"award-number":["82260755"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Journal of Pharmaceutical and Biomedical Analysis"],"published-print":{"date-parts":[[2026,9]]},"DOI":"10.1016\/j.jpba.2026.117539","type":"journal-article","created":{"date-parts":[[2026,4,26]],"date-time":"2026-04-26T14:43:00Z","timestamp":1777214580000},"page":"117539","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":0,"special_numbering":"C","title":["Integrated metabolomics and transcriptomics analysis to reveal the mechanism of Xuefu Zhuyu Decoction in the treatment of nitroglycerin-induced chronic migraine"],"prefix":"10.1016","volume":"278","author":[{"given":"Ting-ting","family":"Zhu","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhu-jun","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ke-ke","family":"Ren","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wen-duan","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Long-jin","family":"Xiao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yu-lin","family":"Feng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shi-lin","family":"Yang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hui","family":"Ouyang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.jpba.2026.117539_bib1","doi-asserted-by":"crossref","first-page":"2461","DOI":"10.1016\/S0140-6736(25)01853-7","article-title":"Global, regional, and national burden of headache disorders, 1990-2023: a systematic analysis for the Global Burden of Disease Study 2023","volume":"406","year":"2025","journal-title":"Lancet"},{"key":"10.1016\/j.jpba.2026.117539_bib2","first-page":"527","article-title":"Rethinking the clinical status of migraine","volume":"44","author":"Song","year":"2023","journal-title":"Clin. Med. Insights"},{"key":"10.1016\/j.jpba.2026.117539_bib3","first-page":"36","article-title":"Relationship between migraine and blood stasis syndrome","author":"Yang","year":"2001","journal-title":"Zhejiang J. Integr. Tradit. Chin. West. Med."},{"key":"10.1016\/j.jpba.2026.117539_bib4","article-title":"Clinical efficacy study of Jiajian Tongqiao Huoxue Decoction in treating migraine with blood stasis obstructing collaterals","author":"Li","year":"2022","journal-title":"Beijing Univ. Chin. Med."},{"key":"10.1016\/j.jpba.2026.117539_bib5","doi-asserted-by":"crossref","first-page":"164","DOI":"10.3988\/jcn.2021.17.2.164","article-title":"The role of endothelial dysfunction in the pathophysiology and cerebrovascular effects of migraine: a narrative review","volume":"17","author":"Paolucci","year":"2021","journal-title":"J. Clin. Neurol."},{"key":"10.1016\/j.jpba.2026.117539_bib6","first-page":"2783","article-title":"Effects of blood residence stasis-resolving decoction combined with cyproheptadine hydrochloride tablets on intracranial arterial hemodynamics, oxygen free radicals, and vasoactive factors in patients with blood stasis obstructing collaterals-type migraine","volume":"25","author":"Dong","year":"2025","journal-title":"Adv. Mod. Biomed. Sci."},{"key":"10.1016\/j.jpba.2026.117539_bib7","first-page":"1790","article-title":"Research progress on migraine pathogenesis and traditional Chinese medicine intervention","volume":"36","author":"Zhao","year":"2025","journal-title":"N. Chin. Drugs Clin."},{"key":"10.1016\/j.jpba.2026.117539_bib8","first-page":"60","article-title":"Study on the correlation between migraine and cerebrovascular disease","volume":"13","author":"Geng","year":"2023","journal-title":"J. Neuropharmacol."},{"key":"10.1016\/j.jpba.2026.117539_bib9","first-page":"46","article-title":"An exploration of Wang Qing-ren's thoughts on promoting blood circulation and removing blood stasis in correction of errors in medical classics","volume":"50","author":"He","year":"2019","journal-title":"Fujian J. Tradit. Chin. Med."},{"key":"10.1016\/j.jpba.2026.117539_bib10","first-page":"1","article-title":"Meta-analysis of the clinical efficacy and safety of Xuefu Zhuyu decoction combined with western medicine in the treatment of migraine","author":"Jang","year":"2025","journal-title":"Tradit. Chin. Med. Pharmacol. Clin."},{"key":"10.1016\/j.jpba.2026.117539_bib11","first-page":"1","article-title":"Research progress on the treatment of headache with blood residence stasis-expelling decoction","author":"Yang","year":"2025","journal-title":"J. Pract. Intern. Med."},{"key":"10.1016\/j.jpba.2026.117539_bib12","article-title":"Effects of blood residence stasis-resolving decoction combined with cyproheptadine hydrochloride tablets on cerebral hemodynamics and vasoactive substances in patients with blood stasis obstructing collaterals-type migraine","volume":"34","author":"Li","year":"2025","journal-title":"J. Mod. Integr. Tradit. Chin. West. Med."},{"key":"10.1016\/j.jpba.2026.117539_bib13","first-page":"1629","article-title":"Regulation of mitochondrial autophagy in rats with blood stasis pattern of coronary heart disease by Xuefu Zhuyu decoction via the PINK1\/Parkin signaling pathway, New Chin","volume":"36","author":"Su","year":"2025","journal-title":"Drugs Clin."},{"key":"10.1016\/j.jpba.2026.117539_bib14","doi-asserted-by":"crossref","first-page":"131","DOI":"10.1186\/s13020-024-01001-0","article-title":"Metabolomics integrated with network pharmacology of blood-entry constituents reveals the bioactive component of Xuefu Zhuyu decoction and its angiogenic effects in treating traumatic brain injury","volume":"19","author":"Li","year":"2024","journal-title":"Chin. Med."},{"key":"10.1016\/j.jpba.2026.117539_bib15","doi-asserted-by":"crossref","first-page":"149","DOI":"10.1007\/s11306-016-1094-6","article-title":"Metabolomics enables precision medicine: \"A White Paper, Community Perspective","volume":"12","author":"Beger","year":"2016","journal-title":"Metabolomics"},{"key":"10.1016\/j.jpba.2026.117539_bib16","doi-asserted-by":"crossref","first-page":"2339","DOI":"10.1002\/hep.32060","article-title":"Metabolomics to predict acute kidney injury in cirrhosis","volume":"74","author":"Piano","year":"2021","journal-title":"Hepatology"},{"key":"10.1016\/j.jpba.2026.117539_bib17","article-title":"A comprehensive analysis of metabolomics and transcriptomics in non-small cell lung cancer","volume":"15","author":"Chen","year":"2020","journal-title":"PLoS One"},{"key":"10.1016\/j.jpba.2026.117539_bib18","doi-asserted-by":"crossref","first-page":"705","DOI":"10.1042\/BST20200652","article-title":"Unlocking immune-mediated disease mechanisms with transcriptomics","volume":"49","author":"DeJong","year":"2021","journal-title":"Biochem. Soc. Trans."},{"key":"10.1016\/j.jpba.2026.117539_bib19","article-title":"Multi-omics analysis reveals the chemoresistance mechanism of proliferating tissue-resident macrophages in PDAC via metabolic adaptation","volume":"42","author":"Zhang","year":"2023","journal-title":"Cell Rep."},{"key":"10.1016\/j.jpba.2026.117539_bib20","doi-asserted-by":"crossref","first-page":"3313","DOI":"10.1007\/s00210-023-02785-y","article-title":"Analysis of the mechanism of curcumin against osteoarthritis using metabolomics and transcriptomics","volume":"397","author":"Deng","year":"2024","journal-title":"Naunyn Schmiede Arch. Pharmacol."},{"key":"10.1016\/j.jpba.2026.117539_bib21","doi-asserted-by":"crossref","first-page":"9.60.1","DOI":"10.1002\/cpns.33","article-title":"Animal model of chronic migraine-associated pain","volume":"80","author":"Moye","year":"2017","journal-title":"Curr. Protoc. Neurosci."},{"key":"10.1016\/j.jpba.2026.117539_bib22","doi-asserted-by":"crossref","first-page":"13","DOI":"10.1186\/s13059-016-0881-8","article-title":"A survey of best practices for RNA-seq data analysis","volume":"17","author":"Conesa","year":"2016","journal-title":"Genome Biol."},{"key":"10.1016\/j.jpba.2026.117539_bib23","doi-asserted-by":"crossref","first-page":"136","DOI":"10.1093\/bioinformatics\/btp612","article-title":"DEGseq: an R package for identifying differentially expressed genes from RNA-seq data","volume":"26","author":"Wang","year":"2010","journal-title":"Bioinformatics"},{"key":"10.1016\/j.jpba.2026.117539_bib24","first-page":"1254","article-title":"Role of hypothalamic neuropeptides in migraine and research progress on related targeted drugs","volume":"20","author":"Wang","year":"2022","journal-title":"J. Integr. Chin. West. Med. Cardiovasc. Cerebrovasc. Dis."},{"key":"10.1016\/j.jpba.2026.117539_bib25","first-page":"102","article-title":"Study on the blood-activating and stasis-resolving effects of Shenghua decoction in an acute blood stasis model of rats","volume":"61","author":"Pu","year":"2025","journal-title":"Food Ferment. Technol."},{"key":"10.1016\/j.jpba.2026.117539_bib26","first-page":"1450","article-title":"Clinical observation of blood stasis-type migraine treated with modified blood residence stasis-resolving decoction combined with cyproheptadine hydrochloride tablets","volume":"23","author":"Shi","year":"2023","journal-title":"Chin. Hosp. Drug Eval. Anal."},{"key":"10.1016\/j.jpba.2026.117539_bib27","first-page":"1188","article-title":"Effects of moxibustion on serum 5-HT and ET-1 levels and brain stem c-fos and c-jun protein expression in migraine rats","volume":"32","author":"Feng","year":"2023","journal-title":"J. Mod. Integr. Tradit. Chin. West. Med."},{"key":"10.1016\/j.jpba.2026.117539_bib28","doi-asserted-by":"crossref","first-page":"164","DOI":"10.3988\/jcn.2021.17.2.164","article-title":"The role of endothelial dysfunction in the pathophysiology and cerebrovascular effects of migraine: a narrative review","volume":"17","author":"Paolucci","year":"2021","journal-title":"J. Clin. Neurol."},{"key":"10.1016\/j.jpba.2026.117539_bib29","first-page":"3622","article-title":"Effects of Tongmai Kaiao pills on angiogenesis and JAK2\/STAT3\/VEGF signaling pathways in rats with vascular cognitive impairment","volume":"47","author":"Liu","year":"2025","journal-title":"Chin. Tradit. Pat. Med."},{"key":"10.1016\/j.jpba.2026.117539_bib30","doi-asserted-by":"crossref","first-page":"119","DOI":"10.1002\/cem.695","article-title":"Orthogonal projections to latent structures (O-PLS)","volume":"16","author":"Trygg","year":"2002","journal-title":"J. Chemom."},{"key":"10.1016\/j.jpba.2026.117539_bib31","first-page":"243","article-title":"Mechanism study on the regulation of NR4A1\/NF-\u03baB by the qi-enhancing, blood-activating, and meridian-unblocking formula to alleviate inflammatory injury in UUO rats","volume":"25","author":"Xu","year":"2023","journal-title":"World Sci. Technol. - Mod. Tradit. Chin. Med."},{"key":"10.1016\/j.jpba.2026.117539_bib32","first-page":"586","article-title":"EGR2 maintains neuropathic pain by promoting microglial phagocytosis","volume":"50","author":"Xi","year":"2025","journal-title":"J. Cent. South Univ."},{"key":"10.1016\/j.jpba.2026.117539_bib33","doi-asserted-by":"crossref","DOI":"10.1016\/j.jep.2022.115470","article-title":"San Pian decoction can treat nitroglycerin-induced migraine in rats by inhibiting the PI3K\/AKT and MAPK signaling pathways","volume":"296","author":"Mao","year":"2022","journal-title":"J. Ethnopharmacol."},{"key":"10.1016\/j.jpba.2026.117539_bib34","first-page":"106","article-title":"Mechanism of action of ephedra-asarum-aconite decoction in migraine intervention based on network pharmacology and in vivo rat experiments","volume":"28","author":"Ge","year":"2022","journal-title":"Chin. J. Exp. Formul."},{"key":"10.1016\/j.jpba.2026.117539_bib35","doi-asserted-by":"crossref","DOI":"10.1155\/2016\/8082753","article-title":"Paeoniflorin and albiflorin attenuate neuropathic pain via MAPK pathway in chronic constriction injury rats","author":"Zhou","year":"2016","journal-title":"Evid. Based Complement. Altern. Med."},{"key":"10.1016\/j.jpba.2026.117539_bib36","doi-asserted-by":"crossref","DOI":"10.1016\/j.jep.2025.121043","article-title":"Puerariae lobatae radix-gastrodiae rhizoma ameliorates nitroglycerin-induced chronic migraine by modulating p38 MAPK\/CREB signaling pathway in the prefrontal cortex-thalamus neural circuit","volume":"358","author":"Wang","year":"2026","journal-title":"J. Ethnopharmacol."}],"container-title":["Journal of Pharmaceutical and Biomedical Analysis"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0731708526002074?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0731708526002074?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,7,3]],"date-time":"2026-07-03T02:32:34Z","timestamp":1783045954000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0731708526002074"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,9]]},"references-count":36,"alternative-id":["S0731708526002074"],"URL":"https:\/\/doi.org\/10.1016\/j.jpba.2026.117539","relation":{},"ISSN":["0731-7085"],"issn-type":[{"value":"0731-7085","type":"print"}],"subject":[],"published":{"date-parts":[[2026,9]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Integrated metabolomics and transcriptomics analysis to reveal the mechanism of Xuefu Zhuyu Decoction in the treatment of nitroglycerin-induced chronic migraine","name":"articletitle","label":"Article Title"},{"value":"Journal of Pharmaceutical and Biomedical Analysis","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.jpba.2026.117539","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2026 Published by Elsevier B.V.","name":"copyright","label":"Copyright"}],"article-number":"117539"}}