{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,15]],"date-time":"2026-07-15T11:10:21Z","timestamp":1784113821262,"version":"3.55.0"},"reference-count":50,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2026,11,1]],"date-time":"2026-11-01T00:00:00Z","timestamp":1793491200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2026,11,1]],"date-time":"2026-11-01T00:00:00Z","timestamp":1793491200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2026,6,14]],"date-time":"2026-06-14T00:00:00Z","timestamp":1781395200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100000024","name":"Canadian Institutes of Health Research","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100000024","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000038","name":"NSERC","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100000038","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Journal of Pharmaceutical and Biomedical Analysis"],"published-print":{"date-parts":[[2026,11]]},"DOI":"10.1016\/j.jpba.2026.117612","type":"journal-article","created":{"date-parts":[[2026,6,14]],"date-time":"2026-06-14T18:34:06Z","timestamp":1781462046000},"page":"117612","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":0,"special_numbering":"C","title":["Development of new liquid chromatography-tandem mass spectrometry methods for the quantification of seven metabolites in three related breast cancer cell lines"],"prefix":"10.1016","volume":"280","author":[{"ORCID":"https:\/\/orcid.org\/0009-0005-9056-1016","authenticated-orcid":false,"given":"Marziyeh","family":"Ghanbarian","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5161-8677","authenticated-orcid":false,"given":"Deborah","family":"Michel","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0005-7137-5701","authenticated-orcid":false,"given":"Maryam","family":"Alyari","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1170-9213","authenticated-orcid":false,"given":"Amir","family":"Khajavinia","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1307-5063","authenticated-orcid":false,"given":"Oleg Y.","family":"Dmitriev","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anas","family":"El-Aneed","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"issue":"6487","key":"10.1016\/j.jpba.2026.117612_bib1","doi-asserted-by":"crossref","DOI":"10.1126\/science.aaw5473","article-title":"Metabolic reprogramming and cancer progression","volume":"368","author":"Faubert","year":"2020","journal-title":"Science"},{"key":"10.1016\/j.jpba.2026.117612_bib2","doi-asserted-by":"crossref","DOI":"10.3389\/fmolb.2021.763902","article-title":"Recent Metabolomics Analysis in Tumor Metabolism Reprogramming","volume":"8","author":"Han","year":"2021","journal-title":"Front. Mol. Biosci."},{"issue":"19","key":"10.1016\/j.jpba.2026.117612_bib3","doi-asserted-by":"crossref","first-page":"3351","DOI":"10.1038\/s41388-020-01639-8","article-title":"The functional roles of TCA cycle metabolites in cancer","volume":"40","author":"Eniafe","year":"2021","journal-title":"Oncogene"},{"key":"10.1016\/j.jpba.2026.117612_bib4","doi-asserted-by":"crossref","first-page":"16","DOI":"10.1038\/s42255-018-0014-7","article-title":"Coupling Krebs cycle metabolites to signalling in immunity and cancer","volume":"1","author":"Ryan","year":"2019","journal-title":"Nat. Metab."},{"issue":"34","key":"10.1016\/j.jpba.2026.117612_bib5","doi-asserted-by":"crossref","first-page":"30409","DOI":"10.1074\/jbc.R200006200","article-title":"The key role of anaplerosis and cataplerosis for citric acid cycle function","volume":"277","author":"Owen","year":"2002","journal-title":"J. Biol. Chem."},{"issue":"12","key":"10.1016\/j.jpba.2026.117612_bib6","doi-asserted-by":"crossref","DOI":"10.3390\/ijms22126587","article-title":"Understanding the Central Role of Citrate in the Metabolism of Cancer Cells and Tumors: An Update","volume":"22","author":"Icard","year":"2021","journal-title":"Int. J. Mol. Sci."},{"issue":"1","key":"10.1016\/j.jpba.2026.117612_bib7","doi-asserted-by":"crossref","first-page":"21","DOI":"10.1007\/s00005-016-0406-x","article-title":"Alpha-Ketoglutarate as a Molecule with Pleiotropic Activity: Well-Known and Novel Possibilities of Therapeutic Use","volume":"65","author":"Zdzisi\u0144ska","year":"2017","journal-title":"Arch. Immunol. Ther. Exp. (Warsz. )"},{"issue":"8","key":"10.1016\/j.jpba.2026.117612_bib8","doi-asserted-by":"crossref","first-page":"4256","DOI":"10.1074\/jbc.M115.688762","article-title":"Fumarate and Succinate Regulate Expression of Hypoxia-inducible Genes via TET Enzymes","volume":"291","author":"Laukka","year":"2016","journal-title":"J. Biol. Chem."},{"issue":"1","key":"10.1016\/j.jpba.2026.117612_bib9","doi-asserted-by":"crossref","first-page":"20","DOI":"10.1186\/s40170-021-00256-7","article-title":"Pyruvate carboxylase and cancer progression","volume":"9","author":"Kiesel","year":"2021","journal-title":"Cancer & Metab."},{"issue":"7443","key":"10.1016\/j.jpba.2026.117612_bib10","doi-asserted-by":"crossref","first-page":"101","DOI":"10.1038\/nature12040","article-title":"Glutamine supports pancreatic cancer growth through a KRAS-regulated metabolic pathway","volume":"496","author":"Son","year":"2013","journal-title":"Nature"},{"issue":"3","key":"10.1016\/j.jpba.2026.117612_bib11","doi-asserted-by":"crossref","first-page":"169","DOI":"10.1016\/j.trecan.2017.01.005","article-title":"Glutamine Metabolism in Cancer: Understanding the Heterogeneity","volume":"3","author":"Cluntun","year":"2017","journal-title":"Trends Cancer"},{"issue":"11","key":"10.1016\/j.jpba.2026.117612_bib12","doi-asserted-by":"crossref","first-page":"630","DOI":"10.1038\/nrcardio.2011.138","article-title":"Metabolomics as a tool for cardiac research","volume":"8","author":"Griffin","year":"2011","journal-title":"Nat. Rev. Cardiol."},{"issue":"3","key":"10.1016\/j.jpba.2026.117612_bib13","doi-asserted-by":"crossref","first-page":"228","DOI":"10.1016\/j.trac.2007.12.001","article-title":"Quantitative metabolomics using NMR","volume":"27","author":"Wishart","year":"2008","journal-title":"TrAC. Trends Anal. Chem."},{"issue":"1","key":"10.1016\/j.jpba.2026.117612_bib14","doi-asserted-by":"crossref","first-page":"51","DOI":"10.1002\/mas.20108","article-title":"Mass spectrometry-based metabolomics","volume":"26","author":"Dettmer","year":"2007","journal-title":"Mass. Spectrom. Rev."},{"issue":"13","key":"10.1016\/j.jpba.2026.117612_bib15","doi-asserted-by":"crossref","first-page":"5527","DOI":"10.1021\/ac100331b","article-title":"High-throughput and multiplexed LC\/MS\/MRM method for targeted metabolomics","volume":"82","author":"Wei","year":"2010","journal-title":"Anal. Chem."},{"key":"10.1016\/j.jpba.2026.117612_bib16","doi-asserted-by":"crossref","first-page":"53","DOI":"10.1016\/j.bbrc.2021.10.072","article-title":"Development of simultaneous quantitative analysis of tricarboxylic acid cycle metabolites to identify specific metabolites in cancer cells by targeted metabolomic approach","volume":"584","author":"Yamamoto","year":"2021","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"10.1016\/j.jpba.2026.117612_bib17","article-title":"Development of a LC-MS\/MS Method for the Simultaneous Detection of Tricarboxylic Acid Cycle Intermediates in a Range of Biological Matrices","author":"Kadhi","year":"2017","journal-title":"J. Anal. Methods Chem. 2017"},{"issue":"4","key":"10.1016\/j.jpba.2026.117612_bib18","doi-asserted-by":"crossref","first-page":"351","DOI":"10.1016\/j.clinbiochem.2005.01.007","article-title":"Contemporary clinical usage of LC\/MS: analysis of biologically important carboxylic acids","volume":"38","author":"Johnson","year":"2005","journal-title":"Clin. Biochem."},{"issue":"1","key":"10.1016\/j.jpba.2026.117612_bib19","doi-asserted-by":"crossref","first-page":"102","DOI":"10.1002\/jssc.201501003","article-title":"Review of in situ derivatization techniques for enhanced bioanalysis using liquid chromatography with mass spectrometry","volume":"39","author":"Baghdady","year":"2016","journal-title":"J. Sep. Sci."},{"key":"10.1016\/j.jpba.2026.117612_bib20","doi-asserted-by":"crossref","first-page":"121","DOI":"10.1016\/j.trac.2014.03.013","article-title":"Derivatization for liquid chromatography-mass spectrometry","volume":"59","author":"Qi","year":"2014","journal-title":"TrAC. Trends Anal. Chem."},{"key":"10.1016\/j.jpba.2026.117612_bib21","doi-asserted-by":"crossref","DOI":"10.1016\/j.jchromb.2023.123719","article-title":"Structural elucidation of 3-nitrophenylhydrazine derivatives of tricarboxylic acid cycle acids and optimization of their fragmentation to boost sensitivity in liquid chromatography-mass spectrometry","volume":"1222","author":"Hodek","year":"2023","journal-title":"J. Chromatogr. B"},{"key":"10.1016\/j.jpba.2026.117612_bib22","doi-asserted-by":"crossref","first-page":"382","DOI":"10.1016\/j.jpba.2018.10.055","article-title":"Improving liquid chromatography-tandem mass spectrometry determination of polycarboxylic acids in human urine by chemical derivatization. Comparison of o-benzyl hydroxylamine and 2-picolyl amine","volume":"164","author":"Gomez-Gomez","year":"2019","journal-title":"J. Pharm. Biomed. Anal."},{"key":"10.1016\/j.jpba.2026.117612_bib23","doi-asserted-by":"crossref","first-page":"134","DOI":"10.1016\/j.ab.2014.07.027","article-title":"Derivatization of the tricarboxylic acid intermediates with O-benzylhydroxylamine for liquid chromatography\u2013tandem mass spectrometry detection","volume":"465","author":"Tan","year":"2014","journal-title":"Anal. Biochem."},{"key":"10.1016\/j.jpba.2026.117612_bib24","doi-asserted-by":"crossref","first-page":"1723","DOI":"10.1007\/s10337-017-3421-0","article-title":"A new derivatization reagent for HPLC\u2013MS analysis of biological organic acids","volume":"80","author":"Marquis","year":"2017","journal-title":"Chromatographia"},{"issue":"2","key":"10.1016\/j.jpba.2026.117612_bib25","doi-asserted-by":"crossref","first-page":"606","DOI":"10.1039\/B701677H","article-title":"Amide bond formation: beyond the myth of coupling reagents","volume":"38","author":"Valeur","year":"2009","journal-title":"Chem. Soc. Rev."},{"key":"10.1016\/j.jpba.2026.117612_bib26","doi-asserted-by":"crossref","DOI":"10.1016\/j.aca.2024.342435","article-title":"Derivatization of carboxylic groups prior to their LC analysis \u2013 A review","volume":"1300","author":"Jankech","year":"2024","journal-title":"Anal. Chim. Acta"},{"key":"10.1016\/j.jpba.2026.117612_bib27","doi-asserted-by":"crossref","first-page":"81","DOI":"10.1016\/j.aca.2018.09.035","article-title":"Quantitative determination of potential urine biomarkers of respiratory illnesses using new targeted metabolomic approach","volume":"1047","author":"Khamis","year":"2019","journal-title":"Anal. Chim. Acta"},{"key":"10.1016\/j.jpba.2026.117612_bib28","doi-asserted-by":"crossref","first-page":"181","DOI":"10.1016\/j.jpba.2016.04.033","article-title":"Isotope-coded ESI-enhancing derivatization reagents for differential analysis, quantification and profiling of metabolites in biological samples by LC\/MS: A review","volume":"130","author":"Higashi","year":"2016","journal-title":"J. Pharm. Biomed. Anal."},{"issue":"3","key":"10.1016\/j.jpba.2026.117612_bib29","doi-asserted-by":"crossref","DOI":"10.1002\/bmc.4756","article-title":"Current trends in isotope-coded derivatization liquid chromatographic-mass spectrometric analyses with special emphasis on their biomedical application","volume":"34","author":"El-Maghrabey","year":"2020","journal-title":"Biomed. Chromatogr."},{"key":"10.1016\/j.jpba.2026.117612_bib30","doi-asserted-by":"crossref","first-page":"29","DOI":"10.1016\/j.jchromb.2019.05.021","article-title":"Development of a new quantification method for organic acids in urine as potential biomarkers for respiratory illness","volume":"1122-1123","author":"Awad","year":"2019","journal-title":"J. Chromatogr. B Anal. Technol. Biomed. Life Sci."},{"key":"10.1016\/j.jpba.2026.117612_bib31","doi-asserted-by":"crossref","DOI":"10.1016\/j.microc.2020.105445","article-title":"Metabolic profiling of material-limited cell samples by dimethylaminophenacyl bromide derivatization with UPLC-MS\/MS analysis","volume":"159","author":"Willacey","year":"2020","journal-title":"Microchem. J."},{"issue":"22","key":"10.1016\/j.jpba.2026.117612_bib32","doi-asserted-by":"crossref","first-page":"13514","DOI":"10.1021\/acs.analchem.8b03435","article-title":"Dansylhydrazine Isotope Labeling LC-MS for Comprehensive Carboxylic Acid Submetabolome Profiling","volume":"90","author":"Zhao","year":"2018","journal-title":"Anal. Chem."},{"issue":"21","key":"10.1016\/j.jpba.2026.117612_bib33","doi-asserted-by":"crossref","first-page":"8789","DOI":"10.1021\/ac102146g","article-title":"High-performance isotope labeling for profiling carboxylic acid-containing metabolites in biofluids by mass spectrometry","volume":"82","author":"Guo","year":"2010","journal-title":"Anal. Chem."},{"key":"10.1016\/j.jpba.2026.117612_bib34","doi-asserted-by":"crossref","DOI":"10.1016\/j.chroma.2019.460413","article-title":"LC\u2013MS\/MS analysis of the central energy and carbon metabolites in biological samples following derivatization by dimethylaminophenacyl bromide","volume":"1608","author":"Willacey","year":"2019","journal-title":"J. Chromatogr. A"},{"issue":"10","key":"10.1016\/j.jpba.2026.117612_bib35","doi-asserted-by":"crossref","first-page":"2079","DOI":"10.1016\/j.tet.2008.12.078","article-title":"Synthesis of 5-aryl-5\u2032-formyl-2,2\u2032-bithiophenes as new precursors for nonlinear optical (NLO) materials","volume":"65","author":"Herbivo","year":"2009","journal-title":"Tetrahedron"},{"issue":"29","key":"10.1016\/j.jpba.2026.117612_bib36","doi-asserted-by":"crossref","first-page":"4831","DOI":"10.1002\/ejoc.200700426","article-title":"Synthesis of 1,1,3-Trisubstituted Naphtho[2,3-c]pyran-5,10-dione Derivatives as Potential Redox Switches","volume":"2007","author":"Shie","year":"2007","journal-title":"Eur. J. Org. Chem."},{"key":"10.1016\/j.jpba.2026.117612_bib37","doi-asserted-by":"crossref","DOI":"10.7554\/eLife.86354","article-title":"MEMO1 binds iron and modulates iron homeostasis in cancer cells","volume":"13","author":"Dolgova","year":"2024","journal-title":"Elife"},{"issue":"3","key":"10.1016\/j.jpba.2026.117612_bib38","doi-asserted-by":"crossref","first-page":"67","DOI":"10.17145\/jab.18.010","article-title":"Bioanalytical method development and validation: from the USFDA 2001 to the USFDA 2018 guidance for industry","volume":"4","author":"Meesters","year":"2018","journal-title":"J. Appl. Bioanal."},{"key":"10.1016\/j.jpba.2026.117612_bib39","author":"Agency","year":"2011","journal-title":"Guidel. Bioanal. Method. Valid. Lond. UK"},{"issue":"25","key":"10.1016\/j.jpba.2026.117612_bib40","doi-asserted-by":"crossref","first-page":"3929","DOI":"10.1016\/j.chroma.2009.11.060","article-title":"Signal suppression\/enhancement in high-performance liquid chromatography tandem mass spectrometry","volume":"1217","author":"Gosetti","year":"2010","journal-title":"J. Chromatogr. A"},{"issue":"7","key":"10.1016\/j.jpba.2026.117612_bib41","doi-asserted-by":"crossref","first-page":"401","DOI":"10.1002\/kin.20012","article-title":"Effect of binary aqueous-organic solvents on the reaction of phenacyl bromide with nitrobenzoic acid (s) in the presence of triethylamine","volume":"36","author":"Nallu","year":"2004","journal-title":"Int. J. Chem. Kinet."},{"issue":"30","key":"10.1016\/j.jpba.2026.117612_bib42","doi-asserted-by":"crossref","first-page":"7419","DOI":"10.1016\/j.tet.2008.05.034","article-title":"\u03b1-Fluorination of \u03b2-ketosulfones by Selectfluor\u2122 F\u2013TEDA\u2013BF4","volume":"64","author":"Loghmani-Khouzani","year":"2008","journal-title":"Tetrahedron"},{"key":"10.1016\/j.jpba.2026.117612_bib43","doi-asserted-by":"crossref","DOI":"10.1016\/j.phrs.2022.106490","article-title":"Therapeutic targeting of glutamate dehydrogenase 1 that links metabolic reprogramming and Snail-mediated epithelial\u2013mesenchymal transition in drug-resistant lung cancer","volume":"185","author":"Wang","year":"2022","journal-title":"Pharmacol. Res."},{"issue":"10","key":"10.1016\/j.jpba.2026.117612_bib44","doi-asserted-by":"crossref","first-page":"525","DOI":"10.1093\/chromsci\/18.10.525","article-title":"Separation of Ionic Compounds by Reversed-Phase Liquid Chromatography An Update of Ion-Pairing Techniques","volume":"18","author":"Bidlingmeyer","year":"1980","journal-title":"J. Chromatogr. Sci."},{"issue":"1","key":"10.1016\/j.jpba.2026.117612_bib45","doi-asserted-by":"crossref","first-page":"227","DOI":"10.1016\/j.jchromb.2007.12.011","article-title":"Development and validation of a liquid chromatographic-tandem mass spectrometric method for determination of piperaquine in plasma: Stable isotope labeled internal standard does not always compensate for matrix effects","volume":"862","author":"Lindegardh","year":"2008","journal-title":"J. Chromatogr. B"},{"issue":"1","key":"10.1016\/j.jpba.2026.117612_bib46","doi-asserted-by":"crossref","first-page":"231","DOI":"10.1007\/s00216-011-5308-5","article-title":"Hydrophilic interaction liquid chromatography (HILIC)--a powerful separation technique","volume":"402","author":"Buszewski","year":"2012","journal-title":"Anal. Bioanal. Chem."},{"issue":"3","key":"10.1016\/j.jpba.2026.117612_bib47","doi-asserted-by":"crossref","first-page":"289","DOI":"10.1002\/jms.984","article-title":"Matrix effects on accurate mass measurements of low-molecular weight compounds using liquid chromatography-electrospray-quadrupole time-of-flight mass spectrometry","volume":"41","author":"Calbiani","year":"2006","journal-title":"J. Mass. Spectrom."},{"key":"10.1016\/j.jpba.2026.117612_bib48","series-title":"LC-MS in drug bioanalysis","author":"Xu","year":"2012"},{"key":"10.1016\/j.jpba.2026.117612_bib49","article-title":"Commission Decision 2002\/657\/EC of 12 August 2002 implementing Council Directive 96\/23\/EC concerning the performance of analytical methods and the interpretation of results","author":"Commission","year":"2002","journal-title":"Off. J. Eur. Communities"},{"issue":"4","key":"10.1016\/j.jpba.2026.117612_bib50","doi-asserted-by":"crossref","DOI":"10.3390\/metabo15040277","article-title":"Metabolic Effects of the Cancer Metastasis Modulator MEMO1","volume":"15","author":"Ghanbarian","year":"2025","journal-title":"Metabolites"}],"container-title":["Journal of Pharmaceutical and Biomedical Analysis"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0731708526002803?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0731708526002803?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,7,15]],"date-time":"2026-07-15T10:56:59Z","timestamp":1784113019000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0731708526002803"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,11]]},"references-count":50,"alternative-id":["S0731708526002803"],"URL":"https:\/\/doi.org\/10.1016\/j.jpba.2026.117612","relation":{},"ISSN":["0731-7085"],"issn-type":[{"value":"0731-7085","type":"print"}],"subject":[],"published":{"date-parts":[[2026,11]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Development of new liquid chromatography-tandem mass spectrometry methods for the quantification of seven metabolites in three related breast cancer cell lines","name":"articletitle","label":"Article Title"},{"value":"Journal of Pharmaceutical and Biomedical Analysis","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.jpba.2026.117612","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2026 The Authors. Published by Elsevier B.V.","name":"copyright","label":"Copyright"}],"article-number":"117612"}}