{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T22:09:07Z","timestamp":1790114947983,"version":"4.0.1"},"reference-count":28,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2026,12,1]],"date-time":"2026-12-01T00:00:00Z","timestamp":1796083200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2026,12,1]],"date-time":"2026-12-01T00:00:00Z","timestamp":1796083200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2026,7,27]],"date-time":"2026-07-27T00:00:00Z","timestamp":1785110400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100018950","name":"CHU Rennes","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100018950","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Journal of Pharmaceutical and Biomedical Analysis"],"published-print":{"date-parts":[[2026,12]]},"DOI":"10.1016\/j.jpba.2026.117669","type":"journal-article","created":{"date-parts":[[2026,7,22]],"date-time":"2026-07-22T23:38:36Z","timestamp":1784763516000},"page":"117669","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":0,"special_numbering":"C","title":["Validation of a rapid semi-automated method for monitoring alcohol abuse by phosphatidylethanol (16:0\/18:1) LC-MS\/MS determination in dried blood spots"],"prefix":"10.1016","volume":"281","author":[{"given":"Emma-Rose","family":"Legrand-Brault","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Romain","family":"Pelletier","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ang\u00e9lique","family":"Cam","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sylvie","family":"Lepage","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Isabelle","family":"Morel","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2354-1884","authenticated-orcid":false,"given":"Thomas","family":"Gicquel","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.jpba.2026.117669_bib1","year":"2024"},{"key":"10.1016\/j.jpba.2026.117669_bib2","doi-asserted-by":"crossref","unstructured":"E. Mayer-Davis, H. Leidy, R. Mattes, T. Naimi, R. Novotny, B. Schneeman, B. James Kingshipp, M. Spill, N. Chong Cole, G. Butera, N. Terry, J. Obbagy, Alcohol Consumption and All-Cause Mortality: A Systematic Review. U.S. Department of Agriculture, Food and Nutrition Service, Center for Nutrition Policy and Promotion, Nutrition Evidence Systematic Review. (2020).","DOI":"10.52570\/NESR.DGAC2020.SR0403"},{"key":"10.1016\/j.jpba.2026.117669_bib3","doi-asserted-by":"crossref","DOI":"10.1001\/jamanetworkopen.2023.6185","article-title":"Association between daily alcohol intake and risk of all-cause mortality: a systematic review and meta-analyses","volume":"6","author":"Zhao","year":"2023","journal-title":"JAMA Netw. Open."},{"key":"10.1016\/j.jpba.2026.117669_bib4","doi-asserted-by":"crossref","first-page":"545","DOI":"10.1080\/03602532.2019.1679169","article-title":"Ethanol and its metabolites: update on toxicity, benefits, and focus on immunomodulatory effects","volume":"51","author":"Le Dar\u00e9","year":"2019","journal-title":"Drug. Metab. Rev."},{"key":"10.1016\/j.jpba.2026.117669_bib5","doi-asserted-by":"crossref","first-page":"1189","DOI":"10.1016\/j.jhep.2025.07.019","article-title":"Phosphatidylethanol in steatotic liver disease","volume":"83","author":"Torp","year":"2025","journal-title":"J. Hepatol."},{"key":"10.1016\/j.jpba.2026.117669_bib6","first-page":"638","article-title":"Phosphatidylethanol blood analysis","volume":"77","author":"Hakim","year":"2019","journal-title":"Ann. De. Biol. Clin."},{"key":"10.1016\/j.jpba.2026.117669_bib7","doi-asserted-by":"crossref","first-page":"1800","DOI":"10.1002\/dta.3340","article-title":"Consensus for the use of the alcohol biomarker phosphatidylethanol (PEth) for the assessment of abstinence and alcohol consumption in clinical and forensic practice (2022 Consensus of Basel)","volume":"14","author":"Luginb\u00fchl","year":"2022","journal-title":"Drug. Test. Anal."},{"key":"10.1016\/j.jpba.2026.117669_bib8","doi-asserted-by":"crossref","first-page":"2292","DOI":"10.1111\/acer.13213","article-title":"The formation, elimination, interpretation, and future research needs of phosphatidylethanol for research studies and clinical practice","volume":"40","author":"Hahn","year":"2016","journal-title":"Alcohol. Clin. Exp. Res."},{"key":"10.1016\/j.jpba.2026.117669_bib9","doi-asserted-by":"crossref","DOI":"10.1002\/jcla.22631","article-title":"Determination of phosphatidylethanol 16:0\/18:1 in whole blood by 96-well supported liquid extraction and UHPLC - MS \/ MS","volume":"33","author":"Berg","year":"2019","journal-title":"Clin. Lab. Anal."},{"key":"10.1016\/j.jpba.2026.117669_bib10","doi-asserted-by":"crossref","first-page":"1395","DOI":"10.1373\/clinchem.2008.120923","article-title":"Molecular species of the alcohol biomarker phosphatidylethanol in human blood measured by LC-MS","volume":"55","author":"Helander","year":"2009","journal-title":"Clin. Chem."},{"key":"10.1016\/j.jpba.2026.117669_bib11","doi-asserted-by":"crossref","first-page":"1293","DOI":"10.1002\/jms.1608","article-title":"Selective detection of phosphatidylethanol homologues in blood as biomarkers for alcohol consumption by LC-ESI-MS\/MS","volume":"44","author":"Gnann","year":"2009","journal-title":"J. Mass. Spectrom."},{"key":"10.1016\/j.jpba.2026.117669_bib12","doi-asserted-by":"crossref","DOI":"10.1016\/j.jchromb.2023.123886","article-title":"Development of an LC-MS\/MS assay with automated sample preparation for phosphatidylethanol (PEth)\u2013 Not your typical clinical marker","volume":"1229","author":"Lahr","year":"2023","journal-title":"J. Chromatogr. B"},{"key":"10.1016\/j.jpba.2026.117669_bib13","doi-asserted-by":"crossref","DOI":"10.1016\/j.jpba.2026.117337","article-title":"Simultaneous determination of three phosphatidylethanol homologues, 12 drugs and metabolites in whole blood by LC\u2013MS\/MS","volume":"273","author":"Maria","year":"2026","journal-title":"J. Pharm. Biomed. Anal."},{"key":"10.1016\/j.jpba.2026.117669_bib14","doi-asserted-by":"crossref","first-page":"825","DOI":"10.1007\/s00216-015-9169-1","article-title":"Quantification of phosphatidylethanol 16:0\/18:1, 18:1\/18:1, and 16:0\/16:0 in venous blood and venous and capillary dried blood spots from patients in alcohol withdrawal and control volunteers","volume":"408","author":"Kummer","year":"2016","journal-title":"Anal. Bioanal. Chem."},{"key":"10.1016\/j.jpba.2026.117669_bib15","doi-asserted-by":"crossref","first-page":"603","DOI":"10.1007\/s00414-012-0799-y","article-title":"Stability of phosphatidylethanol species in spiked and authentic whole blood and matching dried blood spots","volume":"127","author":"Faller","year":"2013","journal-title":"Int. J. Leg. Med."},{"key":"10.1016\/j.jpba.2026.117669_bib16","doi-asserted-by":"crossref","first-page":"1163","DOI":"10.1007\/s00216-011-5221-y","article-title":"LC-MS\/MS analysis of phosphatidylethanol in dried blood spots versus conventional blood specimens","volume":"401","author":"Faller","year":"2011","journal-title":"Anal. Bioanal. Chem."},{"key":"10.1016\/j.jpba.2026.117669_bib17","doi-asserted-by":"crossref","DOI":"10.1016\/j.jpba.2025.117325","article-title":"Device does matter! Comparison of PEth 16:0\/18:1 and PEth 16:0\/18:2 blood concentrations determined from three dried blood spot sampling devices","volume":"271","author":"Hose","year":"2026","journal-title":"J. Pharm. Biomed. Anal."},{"key":"10.1016\/j.jpba.2026.117669_bib18","doi-asserted-by":"crossref","DOI":"10.1002\/bmc.70081","article-title":"Determination by UHPLC-QTOF-HRMS of Phosphatidylethanol (PEth) in Dried Blood Spots: Method Validation and Practical Application of a Rising Alcohol Abuse Biomarker With Minimally Invasive Sampling","volume":"39","author":"Ververi","year":"2025","journal-title":"Biomed. Chromatogr."},{"key":"10.1016\/j.jpba.2026.117669_bib19","first-page":"S106","article-title":"Quantification of phosphatidylethanol combining dried blood spots sampling with Orbitrap-based LC-HRMS platform: Method validation and proficiency testing","volume":"34","author":"D\u00e9glon","year":"2022","journal-title":"Toxicol. Anal. Et. Clin."},{"key":"10.1016\/j.jpba.2026.117669_bib20","doi-asserted-by":"crossref","first-page":"231","DOI":"10.1002\/dta.3695","article-title":"Volumetric dried blood spots for determination of phosphatidylethanol: Validation of a liquid chromatography tandem masspectrometry method and clinical application","volume":"17","author":"Beck","year":"2025","journal-title":"Drug. Test. Anal."},{"key":"10.1016\/j.jpba.2026.117669_bib21","unstructured":"International Council for Harmonisation of Technical Requirements for Pharmaceuticals for Human Use (ICH). ICH Q2(R2) validation of analytical procedures. European Medicines Agency (EMA). Available from: ICH Q2(R2) Validation of analytical procedures - Scientific guideline | European Medicines Agency (EMA). (2024)."},{"key":"10.1016\/j.jpba.2026.117669_bib22","unstructured":"International Council for Harmonisation of Technical Requirements for Pharmaceuticals for Human Use (ICH). ICH M10 guideline on bioanalytical method validation. European Medicines Agency (EMA). Available from: ICH M10 on bioanalytical method validation - Scientific guideline | European Medicines Agency (EMA). (2022)."},{"key":"10.1016\/j.jpba.2026.117669_bib23","unstructured":"ISO 15189:2022. Medical Laboratories - Particular Requirements for Quality and Competence (2022)."},{"key":"10.1016\/j.jpba.2026.117669_bib24","doi-asserted-by":"crossref","first-page":"408","DOI":"10.1016\/j.jpba.2005.11.032","article-title":"Separation of fibrate-type antihyperlipidemic drugs by capillary electrophoresis and their quantitation in pharmaceuticals","volume":"41","author":"Komsta","year":"2006","journal-title":"J. Pharm. Biomed. Anal."},{"key":"10.1016\/j.jpba.2026.117669_bib25","doi-asserted-by":"crossref","first-page":"3019","DOI":"10.1021\/ac020361s","article-title":"Strategies for the Assessment of Matrix Effect in Quantitative Bioanalytical Methods Based on HPLC\u2212MS\/MS","volume":"75","author":"Matuszewski","year":"2003","journal-title":"Anal. Chem."},{"key":"10.1016\/j.jpba.2026.117669_bib26","series-title":"SH GTA 04, R\u00e9vision 02","article-title":"Comit\u00e9 Fran\u00e7ais d\u2032Accr\u00e9ditation (COFRAC). Guide technique d\u2032accr\u00e9ditation de v\u00e9rification (port\u00e9e A)\/ validation (port\u00e9e B) des m\u00e9thodes de biologie m\u00e9dicale","year":"2023"},{"key":"10.1016\/j.jpba.2026.117669_bib27","doi-asserted-by":"crossref","DOI":"10.1016\/j.jchromb.2023.123725","article-title":"Development and Validation of an LC-MS\/MS Method to Quantify the Alcohol Biomarker Phosphatidylethanol 16:0\/18:1 in Dried Blood Spots for Clinical Research Purposes","volume":"1223","author":"Salah","year":"2023","journal-title":"J. Chromatogr. B Anal. Technol. Biomed. Life Sci."},{"key":"10.1016\/j.jpba.2026.117669_bib28","doi-asserted-by":"crossref","first-page":"2013","DOI":"10.1111\/acer.70133","article-title":"Validation of blood phosphatidylethanol as an alcohol consumption biomarker in patients with alcohol use disorder and liver disease at a liver transplant center","volume":"49","author":"Watt","year":"2025","journal-title":"Alcohol. Clin. Exp. Res."}],"container-title":["Journal of Pharmaceutical and Biomedical Analysis"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0731708526003377?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0731708526003377?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T21:47:34Z","timestamp":1790113654000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0731708526003377"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,12]]},"references-count":28,"alternative-id":["S0731708526003377"],"URL":"https:\/\/doi.org\/10.1016\/j.jpba.2026.117669","relation":{},"ISSN":["0731-7085"],"issn-type":[{"value":"0731-7085","type":"print"}],"subject":[],"published":{"date-parts":[[2026,12]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Validation of a rapid semi-automated method for monitoring alcohol abuse by phosphatidylethanol (16:0\/18:1) LC-MS\/MS determination in dried blood spots","name":"articletitle","label":"Article Title"},{"value":"Journal of Pharmaceutical and Biomedical Analysis","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.jpba.2026.117669","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2026 The Authors. Published by Elsevier B.V.","name":"copyright","label":"Copyright"}],"article-number":"117669"}}