{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,19]],"date-time":"2026-09-19T03:03:20Z","timestamp":1789787000657,"version":"4.0.1"},"reference-count":30,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2027,1,1]],"date-time":"2027-01-01T00:00:00Z","timestamp":1798761600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2027,1,1]],"date-time":"2027-01-01T00:00:00Z","timestamp":1798761600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2027,1,1]],"date-time":"2027-01-01T00:00:00Z","timestamp":1798761600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-017"},{"start":{"date-parts":[[2027,1,1]],"date-time":"2027-01-01T00:00:00Z","timestamp":1798761600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2027,1,1]],"date-time":"2027-01-01T00:00:00Z","timestamp":1798761600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-012"},{"start":{"date-parts":[[2027,1,1]],"date-time":"2027-01-01T00:00:00Z","timestamp":1798761600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2027,1,1]],"date-time":"2027-01-01T00:00:00Z","timestamp":1798761600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-004"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82474021"],"award-info":[{"award-number":["82474021"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100007129","name":"Natural Science Foundation of Shandong Province","doi-asserted-by":"publisher","award":["ZR2024MH158"],"award-info":[{"award-number":["ZR2024MH158"]}],"id":[{"id":"10.13039\/501100007129","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Journal of Pharmaceutical and Biomedical Analysis"],"published-print":{"date-parts":[[2027,1]]},"DOI":"10.1016\/j.jpba.2026.117719","type":"journal-article","created":{"date-parts":[[2026,8,29]],"date-time":"2026-08-29T15:19:22Z","timestamp":1788016762000},"page":"117719","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":0,"special_numbering":"C","title":["An ultra-sensitive liquid chromatography-fluorescence detection method for determining hCE1 activity and evaluation of hCE1 as a biomarker of liver injury"],"prefix":"10.1016","volume":"282","author":[{"given":"Xuan","family":"Zhao","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qian-qian","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qi-Chen","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xin","family":"Xu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3493-1615","authenticated-orcid":false,"given":"Dan-Dan","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.jpba.2026.117719_bib1","doi-asserted-by":"crossref","first-page":"335","DOI":"10.4062\/biomolther.2009.17.4.335","article-title":"Carboxylesterases: structure, function and polymorphism","volume":"17","author":"Satoh","year":"2009","journal-title":"Biomol. Ther."},{"key":"10.1016\/j.jpba.2026.117719_bib2","doi-asserted-by":"crossref","first-page":"195","DOI":"10.1016\/j.cbi.2006.07.001","article-title":"Structure, function and regulation of carboxylesterases","volume":"162","author":"Satoh","year":"2006","journal-title":"Chem. Biol. Interact."},{"key":"10.1016\/j.jpba.2026.117719_bib3","doi-asserted-by":"crossref","first-page":"187","DOI":"10.1002\/jbt.20178","article-title":"Human carboxylesterases and their role in xenobiotic and endobiotic metabolism","volume":"21","author":"Ross","year":"2007","journal-title":"J. Biochem. Mol. Toxicol."},{"key":"10.1016\/j.jpba.2026.117719_bib4","doi-asserted-by":"crossref","first-page":"2188","DOI":"10.1002\/hep.25961","article-title":"Deficiency of carboxylesterase 1\/esterase-x results in obesity, hepatic steatosis, and hyperlipidemia","volume":"56","author":"Quiroga","year":"2012","journal-title":"Hepatology"},{"key":"10.1016\/j.jpba.2026.117719_bib5","doi-asserted-by":"crossref","first-page":"1761","DOI":"10.1002\/hep.26714","article-title":"Hepatic carboxylesterase 1 is essential for both normal and farnesoid x receptor-controlled lipid homeostasis","volume":"59","author":"Xu","year":"2014","journal-title":"Hepatology"},{"key":"10.1016\/j.jpba.2026.117719_bib6","doi-asserted-by":"crossref","first-page":"313","DOI":"10.1016\/S1359-6446(05)03383-0","article-title":"Mammalian carboxylesterases: From drug targets to protein therapeutics","volume":"10","author":"Redinbo","year":"2005","journal-title":"Drug. Discov. Today"},{"key":"10.1016\/j.jpba.2026.117719_bib7","doi-asserted-by":"crossref","first-page":"25602","DOI":"10.1074\/jbc.M110.135483","article-title":"Activity-based protein profiling identifies a host enzyme, carboxylesterase 1, which is differentially active during hepatitis c virus replication","volume":"285","author":"Blais","year":"2010","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.jpba.2026.117719_bib8","doi-asserted-by":"crossref","first-page":"3989","DOI":"10.1002\/pmic.200900105","article-title":"Human plasma carboxylesterase 1, a novel serologic biomarker candidate for hepatocellular carcinoma","volume":"9","author":"Na","year":"2009","journal-title":"Proteomics"},{"key":"10.1016\/j.jpba.2026.117719_bib9","doi-asserted-by":"crossref","first-page":"50","DOI":"10.1021\/pr900191p","article-title":"Liverbase: A comprehensive view of human liver biology","volume":"9","author":"Sun","year":"2010","journal-title":"J. Proteome Res."},{"key":"10.1016\/j.jpba.2026.117719_bib10","doi-asserted-by":"crossref","first-page":"575","DOI":"10.1038\/nature13302","article-title":"A draft map of the human proteome","volume":"509","author":"Kim","year":"2014","journal-title":"Nature"},{"key":"10.1016\/j.jpba.2026.117719_bib11","doi-asserted-by":"crossref","first-page":"1022","DOI":"10.1373\/clinchem.2008.102996","article-title":"Abundance of immunologically active alanine aminotransferase in sera of liver cirrhosis and hepatocellular carcinoma patients","volume":"55","author":"Kim","year":"2009","journal-title":"Clin. Chem."},{"key":"10.1016\/j.jpba.2026.117719_bib12","doi-asserted-by":"crossref","first-page":"276","DOI":"10.1093\/toxsci\/kft009","article-title":"Assessment of emerging biomarkers of liver injury in human subjects","volume":"132","author":"Schomaker","year":"2013","journal-title":"Toxicol. Sci."},{"key":"10.1016\/j.jpba.2026.117719_bib13","doi-asserted-by":"crossref","first-page":"75","DOI":"10.1039\/C3TX50077B","article-title":"Safety biomarkers for drug-induced liver injury: Current status and future perspectives","volume":"3","author":"Antoine","year":"2014","journal-title":"Toxicol. Res."},{"key":"10.1016\/j.jpba.2026.117719_bib14","doi-asserted-by":"crossref","first-page":"261","DOI":"10.1093\/clinchem\/48.2.261","article-title":"Serum paraoxonase activity: A new additional test for the improved evaluation of chronic liver damage","volume":"48","author":"Ferre","year":"2002","journal-title":"Clin. Chem."},{"key":"10.1016\/j.jpba.2026.117719_bib15","doi-asserted-by":"crossref","first-page":"2195","DOI":"10.1093\/clinchem\/48.12.2195","article-title":"Diagnostic accuracy of alpha(1)-acid glycoprotein fucosylation for liver cirrhosis in patients undergoing hepatic biopsy","volume":"48","author":"Ryden","year":"2002","journal-title":"Clin. Chem."},{"key":"10.1016\/j.jpba.2026.117719_bib16","doi-asserted-by":"crossref","first-page":"1792","DOI":"10.1373\/clinchem.2007.089144","article-title":"Novel serum biomarker candidates for liver fibrosis in hepatitis C patients","volume":"53","author":"Gangadharan","year":"2007","journal-title":"Clin. Chem."},{"key":"10.1016\/j.jpba.2026.117719_bib17","doi-asserted-by":"crossref","first-page":"1429","DOI":"10.1373\/clinchem.2014.229096","article-title":"YKL-40 and alcoholic liver and pancreas damage and disease in 86,258 individuals from the general population: Cohort and Mendelian randomization studies","volume":"60","author":"Kjaergaard","year":"2014","journal-title":"Clin. Chem."},{"key":"10.1016\/j.jpba.2026.117719_bib18","doi-asserted-by":"crossref","first-page":"1987","DOI":"10.1021\/acssensors.0c00384","article-title":"Bioluminescent sensor reveals that carboxylesterase 1A is a novel endoplasmic reticulum-derived serologic indicator for hepatocyte injury","volume":"5","author":"Wang","year":"2020","journal-title":"ACS Sens."},{"key":"10.1016\/j.jpba.2026.117719_bib19","doi-asserted-by":"crossref","first-page":"1673","DOI":"10.1016\/j.bcp.2005.09.002","article-title":"Butyrylcholinesterase, paraoxonase, and albumin esterase, but not carboxylesterase, are present in human plasma","volume":"70","author":"Li","year":"2005","journal-title":"Biochem. Pharmacol."},{"key":"10.1016\/j.jpba.2026.117719_bib20","doi-asserted-by":"crossref","first-page":"309","DOI":"10.1002\/(SICI)1099-081X(199807)19:5<309::AID-BDD108>3.0.CO;2-9","article-title":"The influence of plasma butyrylcholinesterase concentration on the in vitro hydrolysis of cocaine in human plasma","volume":"19","author":"Browne","year":"1998","journal-title":"Biopharm. Drug. Dispos."},{"key":"10.1016\/j.jpba.2026.117719_bib21","doi-asserted-by":"crossref","first-page":"245","DOI":"10.1194\/jlr.D700022-JLR200","article-title":"Development of an immunoblot assay with infrared fluorescence to quantify paraoxonase 1 in serum and plasma","volume":"49","author":"Connelly","year":"2008","journal-title":"J. Lipid Res."},{"key":"10.1016\/j.jpba.2026.117719_bib22","doi-asserted-by":"crossref","first-page":"745","DOI":"10.1111\/j.2042-7158.1992.tb05512.x","article-title":"Nicotinate esters: Their binding to and hydrolysis by human serum-albumin","volume":"44","author":"Steiner","year":"1992","journal-title":"J. Pharm. Pharmacol."},{"key":"10.1016\/j.jpba.2026.117719_bib23","doi-asserted-by":"crossref","DOI":"10.1126\/science.1260419","article-title":"Tissue-based map of the human proteome","volume":"347","author":"Uhl\u00e9n","year":"2015","journal-title":"Science"},{"key":"10.1016\/j.jpba.2026.117719_bib24","doi-asserted-by":"crossref","first-page":"4302","DOI":"10.1039\/C5RA23614B","article-title":"A highly selective marker reaction for measuring the activity of human carboxylesterase 1 in complex biological samples","volume":"6","author":"Lv","year":"2016","journal-title":"RSC Adv."},{"key":"10.1016\/j.jpba.2026.117719_bib25","doi-asserted-by":"crossref","first-page":"3183","DOI":"10.1039\/C5CC09874B","article-title":"A bioluminescent sensor for highly selective and sensitive detection of human carboxylesterase 1 in complex biological samples","volume":"52","author":"Wang","year":"2016","journal-title":"Chem. Commun."},{"key":"10.1016\/j.jpba.2026.117719_bib26","doi-asserted-by":"crossref","first-page":"14488","DOI":"10.1021\/jacs.5b09854","article-title":"A highly selective ratiometric two-photon fluorescent probe for human cytochrome P450 1A","volume":"137","author":"Dai","year":"2015","journal-title":"J. Am. Chem. Soc."},{"key":"10.1016\/j.jpba.2026.117719_bib27","doi-asserted-by":"crossref","first-page":"1011","DOI":"10.1124\/jpet.104.081265","article-title":"Hydrolysis of capecitabine to 5\u2032-deoxy-5-fluorocytidine by human carboxylesterases and inhibition by loperamide","volume":"313","author":"Quinney","year":"2005","journal-title":"J. Pharmacol. Exp. Ther."},{"key":"10.1016\/j.jpba.2026.117719_bib28","doi-asserted-by":"crossref","first-page":"126","DOI":"10.1124\/dmd.113.053512","article-title":"I. Consortium, In vitro drug metabolism by human carboxylesterase 1: Focus on angiotensin-converting enzyme inhibitors","volume":"42","author":"Thomsen","year":"2014","journal-title":"Drug. Metab. Dispos."},{"key":"10.1016\/j.jpba.2026.117719_bib29","doi-asserted-by":"crossref","first-page":"1477","DOI":"10.1124\/jpet.106.111807","article-title":"Anti-influenza prodrug oseltamivir is activated by carboxylesterase 1, and the activation is inhibited by antiplatelet agent clopidogrel","volume":"319","author":"Shi","year":"2006","journal-title":"J. Pharmacol. Exp. Ther."},{"key":"10.1016\/j.jpba.2026.117719_bib30","doi-asserted-by":"crossref","first-page":"212","DOI":"10.1016\/j.jchromb.2015.11.046","article-title":"Highly sensitive and selective detection of human carboxylesterase 1 activity by liquid chromatography with fluorescence detection","volume":"1008","author":"Wang","year":"2016","journal-title":"J. Chromatogr. B Anal. Technol. Biomed. Life Sci."}],"container-title":["Journal of Pharmaceutical and Biomedical Analysis"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0731708526003870?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0731708526003870?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,9,19]],"date-time":"2026-09-19T02:33:17Z","timestamp":1789785197000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0731708526003870"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2027,1]]},"references-count":30,"alternative-id":["S0731708526003870"],"URL":"https:\/\/doi.org\/10.1016\/j.jpba.2026.117719","relation":{},"ISSN":["0731-7085"],"issn-type":[{"value":"0731-7085","type":"print"}],"subject":[],"published":{"date-parts":[[2027,1]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"An ultra-sensitive liquid chromatography-fluorescence detection method for determining hCE1 activity and evaluation of hCE1 as a biomarker of liver injury","name":"articletitle","label":"Article Title"},{"value":"Journal of Pharmaceutical and Biomedical Analysis","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.jpba.2026.117719","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2026 Published by Elsevier B.V.","name":"copyright","label":"Copyright"}],"article-number":"117719"}}