{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,7]],"date-time":"2026-05-07T23:22:50Z","timestamp":1778196170516,"version":"3.51.4"},"reference-count":50,"publisher":"Elsevier BV","issue":"10","license":[{"start":{"date-parts":[[2025,10,1]],"date-time":"2025-10-01T00:00:00Z","timestamp":1759276800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2025,10,1]],"date-time":"2025-10-01T00:00:00Z","timestamp":1759276800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2025,4,5]],"date-time":"2025-04-05T00:00:00Z","timestamp":1743811200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Journal of Pharmaceutical Analysis"],"published-print":{"date-parts":[[2025,10]]},"DOI":"10.1016\/j.jpha.2025.101299","type":"journal-article","created":{"date-parts":[[2025,5,6]],"date-time":"2025-05-06T11:32:10Z","timestamp":1746531130000},"page":"101299","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":5,"title":["Integrating gas-chromatographical analyses with nuclear-magnetic-resonance spectroscopy to elucidate anti-microbial profile of oleoresins isolated from Rauvolfia serpentina seeds by supercritical\u2013(CO2)\u2013fluid extraction"],"prefix":"10.1016","volume":"15","author":[{"given":"Acharya","family":"Balkrishna","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Monali","family":"Joshi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yash","family":"Varshney","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Manisha","family":"Kabdwal","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Himanshu","family":"Jangid","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.","family":"Priya Rani","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pardeep","family":"Nain","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Savita","family":"Lochab","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anurag","family":"Varshney","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1016\/j.jpha.2025.101299_bib1","first-page":"53","article-title":"Cloning of Rauvolfia serpentina - an endangered medicinal plant","volume":"201","author":"Kataria","year":"2015","journal-title":"J. Sustain. For."},{"key":"10.1016\/j.jpha.2025.101299_bib2","series-title":"Glossary of Indian Medicinal Plants","author":"Chopra","year":"1969"},{"key":"10.1016\/j.jpha.2025.101299_bib3","doi-asserted-by":"crossref","first-page":"350","DOI":"10.1134\/S1607672923700515","article-title":"Anti-aging effect of rutin in Caenorhabditis elegans and D-Gal-induced aging mouse model","volume":"513","author":"Xinghua","year":"2023","journal-title":"Dokl. Biochem. Biophys."},{"key":"10.1016\/j.jpha.2025.101299_bib4","doi-asserted-by":"crossref","first-page":"1177","DOI":"10.48048\/wjst.2020.6492","article-title":"Micropropagation and screening of phytocompounds present among in vitro raised and wild plants of Rauvolfia serpentina","volume":"17","author":"Varnika","year":"2020","journal-title":"Walailak J. Sci. Technol."},{"key":"10.1016\/j.jpha.2025.101299_bib5","doi-asserted-by":"crossref","first-page":"213","DOI":"10.13005\/ojc\/31.Special-Issue1.28","article-title":"Isolation and extraction of flavonoid from the leaves of Rauwolfia serpentina and evaluation of DPPH-scavenging antioxidant potential","volume":"31","author":"Gupta","year":"2015","journal-title":"Orient. J. Chem."},{"key":"10.1016\/j.jpha.2025.101299_bib6","series-title":"In Vitro Production of Secondary Metabolites Reserpine and Ajmalicine in Rauvolfia Serpentina (L.) Benth, Current Aspects in Pharmaceutical Research and Development","first-page":"132","author":"Tiwari","year":"2021"},{"key":"10.1016\/j.jpha.2025.101299_bib7","doi-asserted-by":"crossref","first-page":"741","DOI":"10.1093\/ajh\/hpaa042","article-title":"Reserpine substantially lowers blood pressure in patients with refractory hypertension: A proof-of-concept study","volume":"33","author":"Siddiqui","year":"2020","journal-title":"Am. J. Hypertens."},{"key":"10.1016\/j.jpha.2025.101299_bib8","doi-asserted-by":"crossref","first-page":"2278","DOI":"10.3390\/plants10112278","article-title":"A comparative antibacterial, antioxidant, and antineoplastic potential ofRauwolfia serpentina (L.) leaf extract with its biologically synthesized gold nanoparticles (R-AuNPs)","volume":"10","author":"Alshahrani","year":"2021","journal-title":"Plants"},{"key":"10.1016\/j.jpha.2025.101299_bib9","doi-asserted-by":"crossref","DOI":"10.1016\/j.lfs.2020.118730","article-title":"Reserpine inhibits DNA repair, cell proliferation, invasion and induces apoptosis in oral carcinogenesis via modulation of TGF-\u03b2 signaling","volume":"264","author":"Ramu","year":"2021","journal-title":"Life Sci."},{"key":"10.1016\/j.jpha.2025.101299_bib10","doi-asserted-by":"crossref","first-page":"7968","DOI":"10.3390\/ijms21217968","article-title":"\u03b2-Adrenoceptor activation in breast MCF-10A cells induces a pattern of catecholamine production similar to that of tumorigenic MCF-7 cells","volume":"21","author":"Amaro","year":"2020","journal-title":"Int. J. Mol. Sci."},{"key":"10.1016\/j.jpha.2025.101299_bib11","doi-asserted-by":"crossref","first-page":"629","DOI":"10.1128\/MMBR.00078-15","article-title":"Klebsiella pneumoniae: Going on the offense with a strong defense","volume":"80","author":"Paczosa","year":"2016","journal-title":"Microbiol. Mol. Biol. Rev."},{"key":"10.1016\/j.jpha.2025.101299_bib12","doi-asserted-by":"crossref","first-page":"234","DOI":"10.3390\/antibiotics12020234","article-title":"Carbapenem-resistant Klebsiella pneumoniae: Virulence factors, molecular epidemiology and latest updates in treatment options","volume":"12","author":"Karampatakis","year":"2023","journal-title":"Antibiotics (Basel)"},{"key":"10.1016\/j.jpha.2025.101299_bib13","doi-asserted-by":"crossref","first-page":"23","DOI":"10.1128\/AAC.2.1.23","article-title":"Fatty acids and derivatives as anti-microbial agents","volume":"2","author":"Kabara","year":"1972","journal-title":"Antimicrob. Agents Chemother."},{"key":"10.1016\/j.jpha.2025.101299_bib14","doi-asserted-by":"crossref","first-page":"5485","DOI":"10.3390\/molecules28145485","article-title":"Supercritical fluid extraction (SFE) of polar compounds fromCamellia sinensis leaves: Use of ethanol\/water as a green polarity modifier","volume":"28","author":"Atwi-Ghaddar","year":"2023","journal-title":"Molecules"},{"key":"10.1016\/j.jpha.2025.101299_bib15","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1007\/978-1-62703-529-3_1","article-title":"Bacterial mutation assays","volume":"1044","author":"Zeiger","year":"2013","journal-title":"Methods Mol. Biol."},{"key":"10.1016\/j.jpha.2025.101299_bib16","first-page":"29","article-title":"The Ames Salmonella\/microsome mutagenicity assay","volume":"455","author":"Mortelmans","year":"2000","journal-title":"Mutat. Res."},{"key":"10.1016\/j.jpha.2025.101299_bib17","doi-asserted-by":"crossref","first-page":"21","DOI":"10.1007\/978-1-61779-421-6_2","article-title":"Bacterial mutagenicity assays: Test methods","volume":"817","author":"Gatehouse","year":"2012","journal-title":"Methods Mol. Biol."},{"key":"10.1016\/j.jpha.2025.101299_bib18","doi-asserted-by":"crossref","first-page":"1020","DOI":"10.3390\/molecules26041020","article-title":"Supercritical fluid extract ofPutranjiva roxburghii Wall. seeds mitigates fertility impairment in a zebrafish model","volume":"26","author":"Balkrishna","year":"2021","journal-title":"Molecules"},{"key":"10.1016\/j.jpha.2025.101299_bib19","series-title":"Fats and Fixed Oils (401), United States Pharmacopoeia","year":"2012"},{"key":"10.1016\/j.jpha.2025.101299_bib20","series-title":"Indian Standards 548, Part I","year":"1964"},{"key":"10.1016\/j.jpha.2025.101299_bib21","doi-asserted-by":"crossref","DOI":"10.1128\/JCM.00607-19","article-title":"Development of broth microdilution MIC and disk diffusion anti-microbial susceptibility test quality control ranges for the combination of cefepime and the novel \u03b2-lactamase inhibitor Enmetazobactam","volume":"57","author":"Belley","year":"2019","journal-title":"J. Clin. Microbiol."},{"key":"10.1016\/j.jpha.2025.101299_bib22","series-title":"Anti-microbial Susceptibility Testing Protocols","first-page":"275","article-title":"Methods for determining bactericidal activity and anti-microbial interactions","author":"Verma","year":"2007"},{"key":"10.1016\/j.jpha.2025.101299_bib23","doi-asserted-by":"crossref","first-page":"1429","DOI":"10.3389\/fmicb.2016.01429","article-title":"Development of an in vitro assay, based on the bioFilm ring test\u00ae, for rapid profiling of biofilm-growing bacteria","volume":"7","author":"Di Domenico","year":"2016","journal-title":"Front. Microbiol."},{"key":"10.1016\/j.jpha.2025.101299_bib24","doi-asserted-by":"crossref","first-page":"671","DOI":"10.1038\/nmeth.2089","article-title":"NIH image to imageJ: 25 years of image analysis","volume":"9","author":"Schneider","year":"2012","journal-title":"Nat. Methods"},{"key":"10.1016\/j.jpha.2025.101299_bib25","unstructured":"Test No. 471, Bacterial Reverse Mutation Test, OECD 2020, OECD guidelines for the testing of chemicals. https:\/\/www.oecd-ilibrary.org\/environment\/test-no-471-bacterial-reverse-mutation-test_9789264071247-en. (Accessed 6 October 2024)."},{"key":"10.1016\/j.jpha.2025.101299_bib26","doi-asserted-by":"crossref","first-page":"313","DOI":"10.1080\/1040841X.2016.1208146","article-title":"Critical review on biofilm methods","volume":"43","author":"Azeredo","year":"2017","journal-title":"Crit. Rev. Microbiol."},{"key":"10.1016\/j.jpha.2025.101299_bib27","doi-asserted-by":"crossref","first-page":"1883","DOI":"10.1080\/14786419.2020.1810031","article-title":"Supercritical extraction of volatile and fixed oils fromPetroselinum crispum L. Seeds: Chemical composition and biological activity","volume":"36","author":"Piras","year":"2022","journal-title":"Nat. Prod. Res."},{"key":"10.1016\/j.jpha.2025.101299_bib28","doi-asserted-by":"crossref","first-page":"2607","DOI":"10.1039\/C5FO00325C","article-title":"Supercritical fluid extraction of grape seeds: Extract chemical composition, antioxidant activity and inhibition of nitrite production in LPS-stimulated raw 264.7 cells","volume":"6","author":"Perez","year":"2015","journal-title":"Food Funct."},{"key":"10.1016\/j.jpha.2025.101299_bib29","doi-asserted-by":"crossref","first-page":"185","DOI":"10.3390\/biom10020185","article-title":"Super critical fluid extracted fatty acids fromWithania somnifera seeds repair psoriasis-like skin lesions and attenuate pro-inflammatory cytokines (TNF-\u03b1 and IL-6) release","volume":"10","author":"Balkrishna","year":"2020","journal-title":"Biomolecules"},{"key":"10.1016\/j.jpha.2025.101299_bib30","doi-asserted-by":"crossref","first-page":"694","DOI":"10.1016\/j.bbalip.2011.06.015","article-title":"Oxidized and nitrated oleic acid in biological systems: Analysis by GC-MS\/MS and LC-MS\/MS, and biological significance","volume":"1811","author":"Tsikas","year":"2011","journal-title":"Biochim. Biophys. Acta"},{"key":"10.1016\/j.jpha.2025.101299_bib31","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1038\/s41598-021-87604-1","article-title":"Chemotype of damask rose with oleic acid (9 octadecenoic acid) and its anti-microbial effectiveness","volume":"11","author":"Ghavam","year":"2021","journal-title":"Sci. Rep."},{"key":"10.1016\/j.jpha.2025.101299_bib32","doi-asserted-by":"crossref","first-page":"1285","DOI":"10.1080\/09168451.2020.1731299","article-title":"Linoleic acid, \u03b1-linolenic acid, and monolinolenins as antibacterial substances in the heat-processed soybean fermented with Rhizopus oligosporus","volume":"84","author":"Kusumah","year":"2020","journal-title":"Biosci. Biotechnol. Biochem."},{"key":"10.1016\/j.jpha.2025.101299_bib33","doi-asserted-by":"crossref","first-page":"1293","DOI":"10.17219\/acem\/139398","article-title":"Therapeutic role of vanillin receptors in cancer","volume":"30","author":"Rakoczy","year":"2021","journal-title":"Adv. Clin. Exp. Med."},{"key":"10.1016\/j.jpha.2025.101299_bib34","article-title":"Antibacterial mechanism of vanillin against Escherichia coli O157: H7","volume":"9","author":"Chen","year":"2023","journal-title":"Heliyon"},{"key":"10.1016\/j.jpha.2025.101299_bib35","doi-asserted-by":"crossref","first-page":"755","DOI":"10.3390\/pharmaceutics14040755","article-title":"Anti-inflammatory effect of vanillin protects the stomach against ulcer formation","volume":"14","author":"Ciciliato","year":"2022","journal-title":"Pharmaceutics"},{"key":"10.1016\/j.jpha.2025.101299_bib36","doi-asserted-by":"crossref","DOI":"10.1016\/j.bioadv.2022.213270","article-title":"Effective breast cancer therapy based on palmitic acid-loaded PLGA nanoparticles","volume":"145","author":"He","year":"2023","journal-title":"Biomater. Adv."},{"key":"10.1016\/j.jpha.2025.101299_bib37","doi-asserted-by":"crossref","DOI":"10.1016\/j.jnutbio.2022.109207","article-title":"Dietary squalene supplementation decreases triglyceride species and modifies phospholipid lipidomic profile in the liver of a porcine model of non-alcoholic steatohepatitis","volume":"112","author":"Herrera-Marcos","year":"2023","journal-title":"J. Nutr. Biochem."},{"key":"10.1016\/j.jpha.2025.101299_bib38","doi-asserted-by":"crossref","first-page":"23","DOI":"10.1111\/j.1467-2494.2007.00360.x","article-title":"Protection against singlet oxygen, the main actor of sebum squalene peroxidation during sun exposure, using Commiphora myrrha essential oil","volume":"29","author":"Auffray","year":"2007","journal-title":"Int. J. Cosmet. Sci."},{"key":"10.1016\/j.jpha.2025.101299_bib39","doi-asserted-by":"crossref","first-page":"8133","DOI":"10.2147\/IJN.S221663","article-title":"The droplet-size effect of squalene@cetylpyridinium chloride nanoemulsions on anti-microbial potency against planktonic and biofilm MRSA","volume":"14","author":"Fang","year":"2019","journal-title":"Int. J. Nanomed."},{"key":"10.1016\/j.jpha.2025.101299_bib40","article-title":"Anticancer effect of antioxidant-rich methanolic extract of Rauvolfia serpentina (L.) Benth. Ex Kurz leaves in HepG2 and HeLa cells: A mechanistic insight","volume":"50","author":"Ashim","year":"2023","journal-title":"Biocatal. Agric. Biotechnol."},{"key":"10.1016\/j.jpha.2025.101299_bib41","doi-asserted-by":"crossref","DOI":"10.1002\/cbdv.202401073","article-title":"Molecular docking and antibacterial activity of campesterol derivatives against Staphylococcus aureus, Escherichia coli and Pseudomonas aeruginosa multiresistant strains","volume":"22","author":"de Morais Oliveira-Tintino","year":"2025","journal-title":"Chem. Biodivers."},{"key":"10.1016\/j.jpha.2025.101299_bib42","doi-asserted-by":"crossref","first-page":"113","DOI":"10.1177\/0960327116638727","article-title":"\u03b2-Amyrin, a pentacyclic triterpene, exhibits anti-fibrotic, anti-inflammatory, and anti-apoptotic effects on dimethyl nitrosamine-induced hepatic fibrosis in male rats","volume":"36","author":"Thirupathi","year":"2017","journal-title":"Hum. Exp. Toxicol."},{"key":"10.1016\/j.jpha.2025.101299_bib43","first-page":"320","article-title":"Suppression of EGFR\/STAT3 activity by lupeol contributes to the induction of the apoptosis of human non-small cell lung cancer cells","volume":"55","author":"Min","year":"2019","journal-title":"Int. J. Oncol."},{"key":"10.1016\/j.jpha.2025.101299_bib44","doi-asserted-by":"crossref","DOI":"10.1016\/j.jpha.2023.11.014","article-title":"An integrated strategy of UPLC-Q-TOF\/MS and HPTLC\/PAD-DESI-MSI for the analysis of chemical variations: A case study of Tibetan medicine Tiebangchui","volume":"14","author":"Liu","year":"2024","journal-title":"J. Pharm. Anal."},{"key":"10.1016\/j.jpha.2025.101299_bib45","doi-asserted-by":"crossref","first-page":"148","DOI":"10.1080\/10826076.2020.1866600","article-title":"Scanning densitometry and mass spectrometry for HPTLC analysis of lipids: The last 10 years","volume":"44","author":"Cebolla","year":"2021","journal-title":"J. Liq. Chromatogr. Relat. Technol."},{"key":"10.1016\/j.jpha.2025.101299_bib46","doi-asserted-by":"crossref","first-page":"369","DOI":"10.1007\/s10787-022-01098-5","article-title":"Maltol ameliorates intervertebral disc degeneration through inhibiting PI3K\/AKT\/NF-\u03baB pathway and regulating NLRP3 inflammasome-mediated pyroptosism","volume":"31","author":"Gong","year":"2023","journal-title":"Inflammopharmacology"},{"key":"10.1016\/j.jpha.2025.101299_bib47","doi-asserted-by":"crossref","DOI":"10.1155\/2020\/8348035","article-title":"Vanillic acid alleviates acute myocardial hypoxia\/reoxygenation injury by inhibiting oxidative stress","volume":"2020","author":"Yao","year":"2020","journal-title":"Oxid. Med. Cell. Longev."},{"key":"10.1016\/j.jpha.2025.101299_bib48","doi-asserted-by":"crossref","first-page":"33","DOI":"10.1186\/s40360-023-00676-9","article-title":"Vanillic acid alleviates methamphetamine-induced mitochondrial toxicity in cardiac mitochondria via antioxidant activity and inhibition of MPT Pore opening: An in-vitro study","volume":"24","author":"Shabani","year":"2023","journal-title":"BMC Pharmacol. Toxicol."},{"key":"10.1016\/j.jpha.2025.101299_bib49","doi-asserted-by":"crossref","DOI":"10.1016\/j.plipres.2021.101093","article-title":"Antibacterial fatty acids: An update of possible mechanisms of action and implications in the development of the next-generation of antibacterial agents","volume":"82","author":"Casillas-Vargas","year":"2021","journal-title":"Prog. Lipid Res."},{"key":"10.1016\/j.jpha.2025.101299_bib50","doi-asserted-by":"crossref","first-page":"881","DOI":"10.3201\/eid0809.020063","article-title":"Biofilms: Microbial life on surfaces","volume":"8","author":"Donlan","year":"2002","journal-title":"Emerg. Infect. Dis."}],"container-title":["Journal of Pharmaceutical Analysis"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S2095177925001169?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S2095177925001169?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,1,21]],"date-time":"2026-01-21T00:15:41Z","timestamp":1768954541000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S2095177925001169"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,10]]},"references-count":50,"journal-issue":{"issue":"10","published-print":{"date-parts":[[2025,10]]}},"alternative-id":["S2095177925001169"],"URL":"https:\/\/doi.org\/10.1016\/j.jpha.2025.101299","relation":{},"ISSN":["2095-1779"],"issn-type":[{"value":"2095-1779","type":"print"}],"subject":[],"published":{"date-parts":[[2025,10]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Integrating gas-chromatographical analyses with nuclear-magnetic-resonance spectroscopy to elucidate anti-microbial profile of oleoresins isolated from Rauvolfia serpentina seeds by supercritical\u2013(CO2)\u2013fluid extraction","name":"articletitle","label":"Article Title"},{"value":"Journal of Pharmaceutical Analysis","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.jpha.2025.101299","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2025 The Author(s). Published by Elsevier B.V. on behalf of Xi\u2019an Jiaotong University.","name":"copyright","label":"Copyright"}],"article-number":"101299"}}