{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T18:38:37Z","timestamp":1791916717980,"version":"4.3.4"},"reference-count":39,"publisher":"Elsevier BV","issue":"1","license":[{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2025,6,30]],"date-time":"2025-06-30T00:00:00Z","timestamp":1751241600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100006469","name":"Fundo para o Desenvolvimento das Ci\u00eancias e da Tecnologia","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100006469","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002858","name":"China Postdoctoral Science Foundation","doi-asserted-by":"publisher","award":["GZC20230169"],"award-info":[{"award-number":["GZC20230169"]}],"id":[{"id":"10.13039\/501100002858","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Journal of Pharmaceutical Analysis"],"published-print":{"date-parts":[[2026,1]]},"DOI":"10.1016\/j.jpha.2025.101385","type":"journal-article","created":{"date-parts":[[2025,7,3]],"date-time":"2025-07-03T12:30:26Z","timestamp":1751545826000},"page":"101385","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":2,"title":["FPS_P\/N: A two-dimensional mass spectrometry utilization program with precursor ion determination for accurately distinguishing anthocyanin from other flavonoids"],"prefix":"10.1016","volume":"16","author":[{"given":"Ya-Hui","family":"Ge","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lili","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shilin","family":"Gong","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wen","family":"Miao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Li","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Weibin","family":"Bai","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3875-185X","authenticated-orcid":false,"given":"Jian-Lin","family":"Wu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0404-6431","authenticated-orcid":false,"given":"Na","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.jpha.2025.101385_bib1","doi-asserted-by":"crossref","first-page":"261","DOI":"10.1146\/annurev-food-030216-025923","article-title":"Natural colorants: food colorants from natural sources","volume":"8","author":"Sigurdson","year":"2017","journal-title":"Annu. Rev. Food Sci. Technol."},{"key":"10.1016\/j.jpha.2025.101385_bib2","doi-asserted-by":"crossref","first-page":"314","DOI":"10.1016\/j.freeradbiomed.2011.10.483","article-title":"The anthocyanin cyanidin-3-O-\u03b2-glucoside, a flavonoid, increases hepatic glutathione synthesis and protects hepatocytes against reactive oxygen species during hyperglycemia: involvement of a cAMP-PKA-dependent signaling pathway","volume":"52","author":"Zhu","year":"2012","journal-title":"Free Radic. Biol. Med."},{"key":"10.1016\/j.jpha.2025.101385_bib3","doi-asserted-by":"crossref","first-page":"3809","DOI":"10.3390\/molecules25173809","article-title":"Anthocyanins: a comprehensive review of their chemical properties and health effects on cardiovascular and neurodegenerative diseases","volume":"25","author":"Mattioli","year":"2020","journal-title":"Molecules"},{"key":"10.1016\/j.jpha.2025.101385_bib4","doi-asserted-by":"crossref","first-page":"123","DOI":"10.5455\/jice.20140619030232","article-title":"Structure-activity relationships of antioxidant activity in vitro about flavonoids isolated from Pyrethrum tatsienense","volume":"3","author":"Lin","year":"2014","journal-title":"J. Intercult. Ethnopharmacol."},{"key":"10.1016\/j.jpha.2025.101385_bib5","doi-asserted-by":"crossref","first-page":"1275","DOI":"10.1016\/j.foodchem.2015.09.003","article-title":"Antiradical and reductant activities of anthocyanidins and anthocyanins, structure-activity relationship and synthesis","volume":"194","author":"Ali","year":"2016","journal-title":"Food Chem."},{"key":"10.1016\/j.jpha.2025.101385_bib6","doi-asserted-by":"crossref","DOI":"10.2144\/fsoa-2023-0322","article-title":"Assessment of oral toxicity and safety profile of cyanidin: acute and subacute studies on anthocyanin","volume":"10","author":"Suresh","year":"2024","journal-title":"Future Sci. OA"},{"key":"10.1016\/j.jpha.2025.101385_bib7","doi-asserted-by":"crossref","DOI":"10.1016\/j.afres.2024.100399","article-title":"Beyond colors: the health benefits of maize anthocyanins","volume":"4","author":"Bhushan","year":"2024","journal-title":"Appl. Food Res."},{"key":"10.1016\/j.jpha.2025.101385_bib8","doi-asserted-by":"crossref","first-page":"1619","DOI":"10.1002\/rcm.7584","article-title":"A systematic analysis strategy for accurate detection of anthocyanin pigments in red wines","volume":"30","author":"Li","year":"2016","journal-title":"Rapid Commun. Mass Spectrom."},{"key":"10.1016\/j.jpha.2025.101385_bib9","doi-asserted-by":"crossref","first-page":"10792","DOI":"10.1080\/10408398.2022.2086211","article-title":"Advances in dietary proteins binding with co-existed anthocyanins in foods: Driving forces, structure-affinity relationship, and functional and nutritional properties","volume":"63","author":"Ma","year":"2023","journal-title":"Crit. Rev. Food Sci. Nutr."},{"key":"10.1016\/j.jpha.2025.101385_bib10","doi-asserted-by":"crossref","first-page":"71","DOI":"10.1016\/j.ijms.2011.07.026","article-title":"Structural characterization of cyanidin-3,5-diglucoside and pelargonidin-3,5-diglucoside anthocyanins: multi-dimensional fragmentation pathways using high performance liquid chromatography-electrospray ionization-ion trap-time of flight mass spectrometry","volume":"308","author":"Barnes","year":"2011","journal-title":"Int. J. Mass Spectrom."},{"key":"10.1016\/j.jpha.2025.101385_bib11","doi-asserted-by":"crossref","first-page":"43","DOI":"10.1016\/j.chroma.2008.06.002","article-title":"Simultaneous separation by reversed-phase high-performance liquid chromatography and mass spectral identification of anthocyanins and flavonols in Shiraz grape skin","volume":"1201","author":"Downey","year":"2008","journal-title":"J. Chromatogr. A"},{"key":"10.1016\/j.jpha.2025.101385_bib12","doi-asserted-by":"crossref","first-page":"466","DOI":"10.1016\/j.foodres.2015.05.044","article-title":"Detailed polyphenolic profiling of Annurca apple (M. pumila Miller cv Annurca) by a combination of RP-UHPLC and HILIC, both hyphenated to IT-TOF mass spectrometry","volume":"76","author":"Sommella","year":"2015","journal-title":"Food Res. Int."},{"key":"10.1016\/j.jpha.2025.101385_bib13","doi-asserted-by":"crossref","first-page":"55","DOI":"10.1016\/j.jfca.2016.12.003","article-title":"Retention of polyphenols in blueberries (Vaccinium corymbosum) after different cooking methods, using UHPLC\u2013DAD\u2013MS based metabolomics","volume":"56","author":"Zhao","year":"2017","journal-title":"J. Food Compos. Anal."},{"key":"10.1016\/j.jpha.2025.101385_bib14","doi-asserted-by":"crossref","first-page":"549","DOI":"10.3390\/foods8110549","article-title":"Developing and validating a method for separating flavonoid isomers in common buckwheat sprouts using HPLC-PDA","volume":"8","author":"Jang","year":"2019","journal-title":"Foods"},{"key":"10.1016\/j.jpha.2025.101385_bib15","doi-asserted-by":"crossref","DOI":"10.1155\/2023\/3940081","article-title":"Rapid analysis of 18 flavonoids in tea by ultrahigh-performance liquid chromatography coupled with quadrupole-time of flight mass spectrometry","volume":"2023","author":"Li","year":"2023","journal-title":"J. Food Qual."},{"key":"10.1016\/j.jpha.2025.101385_bib16","doi-asserted-by":"crossref","DOI":"10.1016\/j.foodres.2022.111951","article-title":"Identification and characterization of anthocyanins and non-anthocyanin phenolics from Australian native fruits and their antioxidant, antidiabetic, and anti-Alzheimer potential","volume":"162","author":"Ali","year":"2022","journal-title":"Food Res. Int."},{"key":"10.1016\/j.jpha.2025.101385_bib17","doi-asserted-by":"crossref","first-page":"7085","DOI":"10.1021\/acs.jafc.2c01595","article-title":"MS-FINDER assisted in understanding the profile of flavonoids in temporal dimension during the fermentation of Pu-erh tea","volume":"70","author":"Wang","year":"2022","journal-title":"J. Agric. Food Chem."},{"key":"10.1016\/j.jpha.2025.101385_bib18","doi-asserted-by":"crossref","DOI":"10.1016\/j.foodchem.2021.129008","article-title":"Discovery of the bioactive peptides secreted by Bifidobacterium using integrated MCX coupled with LC-MS and feature-based molecular networking","volume":"347","author":"Chen","year":"2021","journal-title":"Food Chem."},{"key":"10.1016\/j.jpha.2025.101385_bib19","doi-asserted-by":"crossref","first-page":"433","DOI":"10.1002\/pca.2991","article-title":"Electrospray ionisation mass spectrometric behaviour of flavonoid 5-O-glucosides and their positional isomers detected in the extracts from the bark of Prunus cerasus L. and Prunus avium L","volume":"32","author":"Beszterda","year":"2021","journal-title":"Phytochem. Anal."},{"key":"10.1016\/j.jpha.2025.101385_bib20","doi-asserted-by":"crossref","first-page":"1123","DOI":"10.1002\/rcm.6209","article-title":"Study of the mass spectrometric behaviors of anthocyanins in negative ionization mode and its applications for characterization of anthocyanins and non-anthocyanin polyphenols","volume":"26","author":"Sun","year":"2012","journal-title":"Rapid Commun. Mass Spectrom."},{"key":"10.1016\/j.jpha.2025.101385_bib21","doi-asserted-by":"crossref","first-page":"12206","DOI":"10.1021\/acs.jafc.8b05029","article-title":"Nontargeted metabolomic study on variation of phenolics in different cranberry cultivars using UPLC-IM-HRMS","volume":"66","author":"Wang","year":"2018","journal-title":"J. Agric. Food Chem."},{"key":"10.1016\/j.jpha.2025.101385_bib22","doi-asserted-by":"crossref","first-page":"179","DOI":"10.1016\/j.aca.2018.11.040","article-title":"Fingerprinting of traditionally produced red wines using liquid chromatography combined with drift tube ion mobility-mass spectrometry","volume":"1052","author":"Causon","year":"2019","journal-title":"Anal. Chim. Acta"},{"key":"10.1016\/j.jpha.2025.101385_bib23","doi-asserted-by":"crossref","first-page":"4039","DOI":"10.1021\/acs.jpca.1c00971","article-title":"Efficient quantum chemical calculation of structure ensembles and free energies for nonrigid molecules","volume":"125","author":"Grimme","year":"2021","journal-title":"J. Phys. Chem. A"},{"key":"10.1016\/j.jpha.2025.101385_bib24","doi-asserted-by":"crossref","first-page":"73","DOI":"10.1002\/wcms.81","article-title":"The ORCA program system","volume":"2","author":"Neese","year":"2012","journal-title":"Wiley Interdiscip. Rev. Comput. Mol. Sci."},{"key":"10.1016\/j.jpha.2025.101385_bib25","doi-asserted-by":"crossref","first-page":"23","DOI":"10.1038\/s43586-023-00203-4","article-title":"Mass spectrometry using electrospray ionization","volume":"3","author":"Prabhu","year":"2023","journal-title":"Nat. Rev. Methods Primers"},{"key":"10.1016\/j.jpha.2025.101385_bib26","doi-asserted-by":"crossref","first-page":"566","DOI":"10.1002\/mas.20027","article-title":"Internal energy and fragmentation of ions produced in electrospray sources","volume":"24","author":"Gabelica","year":"2005","journal-title":"Mass Spectrom. Rev."},{"key":"10.1016\/j.jpha.2025.101385_bib27","doi-asserted-by":"crossref","first-page":"478","DOI":"10.1002\/jms.1914","article-title":"In-source fragmentation and accurate mass analysis of multiclass flavonoid conjugates by electrospray ionization time-of-flight mass spectrometry","volume":"46","author":"Abrank\u00f3","year":"2011","journal-title":"J. Mass Spectrom."},{"key":"10.1016\/j.jpha.2025.101385_bib28","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1002\/jms.585","article-title":"Mass spectrometry in the structural analysis of flavonoids","volume":"39","author":"Cuyckens","year":"2004","journal-title":"J. Mass Spectrom."},{"key":"10.1016\/j.jpha.2025.101385_bib29","doi-asserted-by":"crossref","first-page":"126","DOI":"10.1080\/10408347.2011.632314","article-title":"Analysis and antioxidant capacity of anthocyanin pigments. Part II: chemical structure, color, and intake of anthocyanins","volume":"42","author":"Bueno","year":"2012","journal-title":"Crit. Rev. Anal. Chem."},{"key":"10.1016\/j.jpha.2025.101385_bib30","first-page":"163","article-title":"Optimization and kinetic studies of ultrasound-assisted extraction on polyphenols from Satsuma Mandarin (Citrus Unshiu Marc.) leaves","volume":"36","author":"Ci\u011fero\u011flu","year":"2017","journal-title":"Iran. J. Chem. Chem. Eng."},{"key":"10.1016\/j.jpha.2025.101385_bib31","doi-asserted-by":"crossref","DOI":"10.1016\/j.foodchem.2019.125745","article-title":"Simultaneous determination of multiclass illegal dyes with different acidic-basic properties in foodstuffs by LC-MS\/MS via polarity switching mode","volume":"309","author":"Hu","year":"2020","journal-title":"Food Chem."},{"key":"10.1016\/j.jpha.2025.101385_bib32","doi-asserted-by":"crossref","first-page":"4612","DOI":"10.1016\/j.chroma.2010.05.007","article-title":"Determination of anthelmintic drug residues in milk using ultra high performance liquid chromatography-tandem mass spectrometry with rapid polarity switching","volume":"1217","author":"Whelan","year":"2010","journal-title":"J. Chromatogr. A"},{"key":"10.1016\/j.jpha.2025.101385_bib33","doi-asserted-by":"crossref","first-page":"68","DOI":"10.1016\/j.chroma.2018.12.033","article-title":"A single run, rapid polarity switching method for determination of 30 pharmaceuticals and personal care products in waste water using Q-Exactive Orbitrap high resolution accurate mass spectrometry","volume":"1588","author":"Abou-Elwafa Abdallah","year":"2019","journal-title":"J. Chromatogr. A"},{"key":"10.1016\/j.jpha.2025.101385_bib34","doi-asserted-by":"crossref","first-page":"77","DOI":"10.1016\/j.talanta.2014.08.011","article-title":"Rapid analysis of trace organic compounds in water by automated online solid-phase extraction coupled to liquid chromatography-tandem mass spectrometry","volume":"132","author":"Anumol","year":"2015","journal-title":"Talanta"},{"key":"10.1016\/j.jpha.2025.101385_bib35","doi-asserted-by":"crossref","DOI":"10.1016\/j.jpba.2021.113931","article-title":"In vivo and in vitro studies of Banhahoobak-tang tablets using UPLC-ESI-MS\/MS with polarity switching","volume":"196","author":"Jeong","year":"2021","journal-title":"J. Pharm. Biomed. Anal."},{"key":"10.1016\/j.jpha.2025.101385_bib36","doi-asserted-by":"crossref","first-page":"1582","DOI":"10.1021\/acs.jafc.6b05455","article-title":"Flavonoid metabolites in human urine during blueberry anthocyanin intake","volume":"65","author":"Kalt","year":"2017","journal-title":"J. Agric. Food Chem."},{"key":"10.1016\/j.jpha.2025.101385_bib37","doi-asserted-by":"crossref","first-page":"336","DOI":"10.1016\/j.foodchem.2018.10.101","article-title":"Simultaneous LC-MS quantification of anthocyanins and non-anthocyanin phenolics from blueberries with widely divergent profiles and biological activities","volume":"277","author":"Grace","year":"2019","journal-title":"Food Chem."},{"key":"10.1016\/j.jpha.2025.101385_bib38","doi-asserted-by":"crossref","DOI":"10.1016\/j.dyepig.2020.108852","article-title":"Acylation of blueberry anthocyanins with maleic acid: improvement of the stability and its application potential in intelligent color indicator packing materials","volume":"184","author":"Fei","year":"2021","journal-title":"Dyes Pigments"},{"key":"10.1016\/j.jpha.2025.101385_bib39","doi-asserted-by":"crossref","first-page":"119","DOI":"10.1016\/j.foodchem.2016.07.073","article-title":"Stability-increasing effects of anthocyanin glycosyl acylation","volume":"214","author":"Zhao","year":"2017","journal-title":"Food Chem"}],"container-title":["Journal of Pharmaceutical Analysis"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S2095177925002023?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S2095177925002023?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,4,14]],"date-time":"2026-04-14T11:32:31Z","timestamp":1776166351000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S2095177925002023"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,1]]},"references-count":39,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2026,1]]}},"alternative-id":["S2095177925002023"],"URL":"https:\/\/doi.org\/10.1016\/j.jpha.2025.101385","relation":{},"ISSN":["2095-1779"],"issn-type":[{"value":"2095-1779","type":"print"}],"subject":[],"published":{"date-parts":[[2026,1]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"FPS_P\/N: A two-dimensional mass spectrometry utilization program with precursor ion determination for accurately distinguishing anthocyanin from other flavonoids","name":"articletitle","label":"Article Title"},{"value":"Journal of Pharmaceutical Analysis","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.jpha.2025.101385","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2025 The Author(s). Published by Elsevier B.V. on behalf of Xi\u2019an Jiaotong University.","name":"copyright","label":"Copyright"}],"article-number":"101385"}}