{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,8]],"date-time":"2026-04-08T14:30:47Z","timestamp":1775658647861,"version":"3.50.1"},"reference-count":40,"publisher":"Elsevier BV","issue":"4","license":[{"start":{"date-parts":[[2016,12,1]],"date-time":"2016-12-01T00:00:00Z","timestamp":1480550400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2016,3,23]],"date-time":"2016-03-23T00:00:00Z","timestamp":1458691200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Journal of Pharmacological Sciences"],"published-print":{"date-parts":[[2016,12]]},"DOI":"10.1016\/j.jphs.2016.03.008","type":"journal-article","created":{"date-parts":[[2016,3,26]],"date-time":"2016-03-26T11:52:02Z","timestamp":1458993122000},"page":"218-223","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":10,"title":["Inhibition of extracellular matrix production and remodeling by doxycycline in smooth muscle cells"],"prefix":"10.1016","volume":"132","author":[{"given":"Rogelio","family":"Palomino-Morales","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Carolina","family":"Torres","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sonia","family":"Perales","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ana","family":"Linares","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maria Jose","family":"Alejandre","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1016\/j.jphs.2016.03.008_bib1","doi-asserted-by":"crossref","first-page":"12","DOI":"10.1177\/08959374980120010501","article-title":"Tetracyclines inhibit connective tissue breakdown by multiple non-antimicrobial mechanisms","volume":"12","author":"Golub","year":"1998","journal-title":"Adv Dent Res"},{"key":"10.1016\/j.jphs.2016.03.008_bib2","doi-asserted-by":"crossref","first-page":"1082","DOI":"10.1016\/S0741-5214(98)70035-7","article-title":"Pharmacologic suppression of experimental abdominal aortic aneurysms: a comparison of doxycycline and four chemically modified tetracyclines","volume":"28","author":"Curci","year":"1998","journal-title":"J\u00a0Vasc Surg"},{"key":"10.1016\/j.jphs.2016.03.008_bib3","doi-asserted-by":"crossref","first-page":"923","DOI":"10.1067\/mva.2002.123757","article-title":"Doxycycline in patients with abdominal aortic aneurysms and in mice: comparison of serum levels and effect on aneurysm growth in mice","volume":"35","author":"Prall","year":"2002","journal-title":"J\u00a0Vasc Surg"},{"key":"10.1016\/j.jphs.2016.03.008_bib4","doi-asserted-by":"crossref","first-page":"740","DOI":"10.1016\/0006-291X(92)91118-A","article-title":"Selective endothelial growth inhibition by tetracyclines that inhibit collagenase","volume":"188","author":"Guerin","year":"1992","journal-title":"Biochem Biophys Res Commun"},{"key":"10.1016\/j.jphs.2016.03.008_bib5","doi-asserted-by":"crossref","first-page":"364","DOI":"10.1161\/01.RES.0000138581.04174.2f","article-title":"Minocycline exerts multiple inhibitory effects on vascular endothelial growth factor-induced smooth muscle cell migration: the role of ERK1\/2, PI3K, and matrix metalloproteinases","volume":"95","author":"Yao","year":"2004","journal-title":"Circ Res"},{"key":"10.1016\/j.jphs.2016.03.008_bib6","doi-asserted-by":"crossref","first-page":"1089","DOI":"10.1016\/S0002-9440(10)64929-2","article-title":"Doxycycline modulates smooth muscle cell growth, migration, and matrix remodeling after arterial injury","volume":"160","author":"Bendeck","year":"2002","journal-title":"Am J Pathol"},{"key":"10.1016\/j.jphs.2016.03.008_bib7","doi-asserted-by":"crossref","first-page":"547","DOI":"10.1111\/j.1749-6632.1999.tb07723.x","article-title":"MMP inhibition reduces intimal hyperplasia in a human vein graft stenosis model","volume":"878","author":"Loftus","year":"1999","journal-title":"Ann NY Acad Sci"},{"key":"10.1016\/j.jphs.2016.03.008_bib8","doi-asserted-by":"crossref","first-page":"314","DOI":"10.1111\/j.1471-4159.2005.03520.x","article-title":"Minocycline exerts inhibitory effects on multiple mitogen-activated protein kinases and IkappaBalpha degradation in a stimulus-specific manner in microglia","volume":"96","author":"Nikodemova","year":"2006","journal-title":"J\u00a0Neurochem"},{"key":"10.1016\/j.jphs.2016.03.008_bib9","doi-asserted-by":"crossref","first-page":"467","DOI":"10.1091\/mbc.11.2.467","article-title":"Rab7: a key to lysosome biogenesis","volume":"11","author":"Bucci","year":"2000","journal-title":"Mol Biol Cell"},{"key":"10.1016\/j.jphs.2016.03.008_bib10","doi-asserted-by":"crossref","first-page":"767","DOI":"10.1038\/nrm1229","article-title":"Ras GTPases: integrins' friends or foes?","volume":"4","author":"Kinbara","year":"2003","journal-title":"Nat Rev Mol Cell Biol"},{"key":"10.1016\/j.jphs.2016.03.008_bib11","doi-asserted-by":"crossref","first-page":"1704","DOI":"10.1126\/science.1092053","article-title":"Cell migration: integrating signals from front to back","volume":"302","author":"Ridley","year":"2003","journal-title":"Science"},{"key":"10.1016\/j.jphs.2016.03.008_bib12","doi-asserted-by":"crossref","first-page":"563","DOI":"10.1016\/j.cub.2005.07.010","article-title":"Signaling interplay in Ras superfamily function","volume":"15","author":"Mitin","year":"2005","journal-title":"Curr Biol"},{"key":"10.1016\/j.jphs.2016.03.008_bib13","doi-asserted-by":"crossref","first-page":"757","DOI":"10.1161\/01.RES.0000210579.35304.d3","article-title":"Rho GTPases and leukocyte adhesion receptor expression and function in endothelial cells","volume":"98","author":"Cernuda-Morollon","year":"2006","journal-title":"Circ Res"},{"key":"10.1016\/j.jphs.2016.03.008_bib14","doi-asserted-by":"crossref","first-page":"651","DOI":"10.1111\/j.1469-7793.2001.t01-2-00651.x","article-title":"Thiophosphorylation-induced Ca(2+) sensitization of guinea-pig ileum contractility is not mediated by Rho-associated kinase","volume":"533","author":"Pfitzer","year":"2001","journal-title":"J\u00a0Physiol"},{"key":"10.1016\/j.jphs.2016.03.008_bib15","doi-asserted-by":"crossref","first-page":"577","DOI":"10.1016\/S0022-2828(03)00110-X","article-title":"Serum response factor: toggling between disparate programs of gene expression","volume":"35","author":"Miano","year":"2003","journal-title":"J\u00a0Mol Cell Cardiol"},{"key":"10.1016\/j.jphs.2016.03.008_bib16","doi-asserted-by":"crossref","first-page":"39","DOI":"10.1165\/rcmb.2002-0206OC","article-title":"The RhoA\/Rho kinase pathway regulates nuclear localization of serum response factor","volume":"29","author":"Liu","year":"2003","journal-title":"Am J Respir Cell Mol Biol"},{"key":"10.1016\/j.jphs.2016.03.008_bib17","doi-asserted-by":"crossref","first-page":"1051","DOI":"10.1152\/ajpheart.00710.2004","article-title":"RhoA and resistance artery remodeling","volume":"288","author":"Loirand","year":"2005","journal-title":"Am J Physiol Heart Circ Physiol"},{"key":"10.1016\/j.jphs.2016.03.008_bib18","doi-asserted-by":"crossref","first-page":"151","DOI":"10.2174\/187152906778249545","article-title":"Nutritional control, gene regulation, and transformation of vascular smooth muscle cells in atherosclerosis","volume":"6","author":"Linares","year":"2006","journal-title":"Cardiovasc Hematol Disord Drug Targets"},{"key":"10.1016\/j.jphs.2016.03.008_bib19","doi-asserted-by":"crossref","first-page":"181","DOI":"10.1007\/s11745-998-0194-x","article-title":"Changes in cultured arterial smooth muscle cells isolated from chicks upon cholesterol feeding","volume":"33","author":"Carazo","year":"1998","journal-title":"Lipids"},{"key":"10.1016\/j.jphs.2016.03.008_bib20","doi-asserted-by":"crossref","first-page":"5134","DOI":"10.1016\/S0021-9258(19)70760-1","article-title":"Relation of cholesterol and mevalonic acid to the cell cycle in smooth muscle and swiss 3T3 cells stimulated to divide by platelet-derived growth factor","volume":"255","author":"Habenicht","year":"1980","journal-title":"J\u00a0Biol Chem"},{"key":"10.1016\/j.jphs.2016.03.008_bib21","doi-asserted-by":"crossref","first-page":"172","DOI":"10.1083\/jcb.50.1.172","article-title":"The smooth muscle cell. II. Growth of smooth muscle in culture and formation of elastic fibers","volume":"50","author":"Ross","year":"1971","journal-title":"J\u00a0Cell Biol"},{"key":"10.1016\/j.jphs.2016.03.008_bib22","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1152\/physrev.1979.59.1.1","article-title":"The smooth muscle cell in culture","volume":"59","author":"Chamley-Campbell","year":"1979","journal-title":"Physiol Rev"},{"key":"10.1016\/j.jphs.2016.03.008_bib23","doi-asserted-by":"crossref","first-page":"1697","DOI":"10.2353\/ajpath.2006.050613","article-title":"Doxycycline alters vascular smooth muscle cell adhesion, migration, and reorganization of fibrillar collagen matrices","volume":"168","author":"Franco","year":"2006","journal-title":"Am J Pathol"},{"key":"10.1016\/j.jphs.2016.03.008_bib24","doi-asserted-by":"crossref","first-page":"1376","DOI":"10.1016\/S0741-5214(03)01022-X","article-title":"Mechanism of inhibition of matrix metalloproteinase-2 expression by doxycycline in human aortic smooth muscle cells","volume":"38","author":"Liu","year":"2003","journal-title":"J\u00a0Vasc Surg"},{"key":"10.1016\/j.jphs.2016.03.008_bib25","doi-asserted-by":"crossref","first-page":"1189","DOI":"10.1016\/S0889-8588(02)00044-8","article-title":"Development of matrix metalloproteinase inhibitors in cancer therapy","volume":"16","author":"Purcell","year":"2002","journal-title":"Hematol Oncol Clin North Am"},{"key":"10.1016\/j.jphs.2016.03.008_bib26","doi-asserted-by":"crossref","first-page":"988","DOI":"10.1021\/bi00782a009","article-title":"Use of a mixture of proteinase-free collagenases for specific assay of radioactive collagen in presence of other proteins","volume":"10","author":"Peterkof","year":"1971","journal-title":"Biochemistry"},{"key":"10.1016\/j.jphs.2016.03.008_bib27","doi-asserted-by":"crossref","first-page":"248","DOI":"10.1016\/0003-2697(76)90527-3","article-title":"A\u00a0rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding","volume":"72","author":"Bradford","year":"1976","journal-title":"Anal Biochem"},{"key":"10.1016\/j.jphs.2016.03.008_bib28","first-page":"29","article-title":"A\u00a0new mathematical model for relative quantification in real-time RT-PCR","author":"Pfaffl","year":"2001","journal-title":"Nucleic Acids Res"},{"key":"10.1016\/j.jphs.2016.03.008_bib29","doi-asserted-by":"crossref","first-page":"798","DOI":"10.1002\/art.10953","article-title":"Selective stimulation of collagen synthesis in the presence of costimulatory insulin signaling by connective tissue growth factor in scleroderma fibroblasts","volume":"48","author":"Gore-Hyer","year":"2003","journal-title":"Arthritis Rheum"},{"key":"10.1016\/j.jphs.2016.03.008_bib30","article-title":"Cholesterol loading in\u00a0vivo and in\u00a0vitro alters extracellular matrix proteins production in smooth muscle cells","volume":"117","author":"Palomino-Morales","year":"2015","journal-title":"Eur J Lipid Sci Technol"},{"key":"10.1016\/j.jphs.2016.03.008_bib31","doi-asserted-by":"crossref","first-page":"733","DOI":"10.1161\/01.ATV.0000121571.78696.dc","article-title":"Clinical and biochemical results of the metalloproteinase inhibition with subantimicrobial doses of doxycycline to prevent acute coronary syndromes (MIDAS) pilot trial","volume":"24","author":"Brown","year":"2004","journal-title":"Arterioscler Thromb Vasc Biol"},{"key":"10.1016\/j.jphs.2016.03.008_bib32","doi-asserted-by":"crossref","first-page":"2858","DOI":"10.1161\/01.STR.0000038098.04291.F6","article-title":"Prospective, randomized, double-blind trial investigating the effect of doxycycline on matrix metalloproteinase expression within atherosclerotic carotid plaques","volume":"33","author":"Axisa","year":"2002","journal-title":"Stroke"},{"key":"10.1016\/j.jphs.2016.03.008_bib33","doi-asserted-by":"crossref","first-page":"1557","DOI":"10.1016\/S0002-9440(10)63512-2","article-title":"A\u00a0nonantibiotic chemically modified tetracycline (CMT-3) inhibits intimal thickening","volume":"163","author":"Islam","year":"2003","journal-title":"Am J Pathol"},{"key":"10.1016\/j.jphs.2016.03.008_bib34","doi-asserted-by":"crossref","first-page":"157","DOI":"10.1159\/000077145","article-title":"Inward remodeling of the rabbit aorta is blocked by the matrix metalloproteinase inhibitor doxycycline","volume":"41","author":"Courtman","year":"2004","journal-title":"J\u00a0Vasc Res"},{"key":"10.1016\/j.jphs.2016.03.008_bib35","doi-asserted-by":"crossref","first-page":"528","DOI":"10.1006\/dbio.1993.1261","article-title":"Type X collagen degradation in long-term serum-free culture of the embryonic chick tibia following production of active collagenase and gelatinase","volume":"159","author":"Cole","year":"1993","journal-title":"Dev Biol"},{"key":"10.1016\/j.jphs.2016.03.008_bib36","doi-asserted-by":"crossref","first-page":"63","DOI":"10.1177\/08959374980120012201","article-title":"Doxycycline inhibits collagen synthesis by differentiated articular chondrocytes","volume":"12","author":"TeKoppele","year":"1998","journal-title":"Adv Dent Res"},{"key":"10.1016\/j.jphs.2016.03.008_bib37","doi-asserted-by":"crossref","unstructured":"Rogelio Palomino-Morales, Sonia Perales, Carolina Torres, Ana Linares and Maria Jose Alejandre. Effect of HMG-CoA Reductase Inhibition on Vascular Smooth Muscle Cells Extracellular Matrix production: Role of RhoA. Current Vascular Pharmacology. Accepted.","DOI":"10.2174\/1570161114666160229115553"},{"key":"10.1016\/j.jphs.2016.03.008_bib38","doi-asserted-by":"crossref","first-page":"76","DOI":"10.1161\/hq0102.101822","article-title":"Shear stress activation of SREBP1 in endothelial cells is mediated by Integrins","volume":"22","author":"Liu","year":"2002","journal-title":"Arteriosclerosis Thrombosis Vasc Biol"},{"key":"10.1016\/j.jphs.2016.03.008_bib39","doi-asserted-by":"crossref","first-page":"329","DOI":"10.1152\/ajprenal.00136.2011","article-title":"SREBP-1 activation by glucose mediates TGF-beta upregulation in mesangial cells","volume":"302","author":"Uttarwar","year":"2012","journal-title":"Am J Physiol Ren Physiol"},{"key":"10.1016\/j.jphs.2016.03.008_bib40","doi-asserted-by":"crossref","first-page":"299","DOI":"10.1152\/ajplung.00271.2013","article-title":"Peroxisome proliferator activated receptor-gamma-Rho-kinase interactions contribute to vascular remodeling after chronic intrauterine pulmonary hypertension","volume":"306","author":"Gien","year":"2014","journal-title":"Am J Physiol Lung Cell Mol Physiol"}],"container-title":["Journal of Pharmacological Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1347861316300020?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1347861316300020?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2022,6,16]],"date-time":"2022-06-16T07:13:05Z","timestamp":1655363585000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S1347861316300020"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016,12]]},"references-count":40,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2016,12]]}},"alternative-id":["S1347861316300020"],"URL":"https:\/\/doi.org\/10.1016\/j.jphs.2016.03.008","relation":{},"ISSN":["1347-8613"],"issn-type":[{"value":"1347-8613","type":"print"}],"subject":[],"published":{"date-parts":[[2016,12]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Inhibition of extracellular matrix production and remodeling by doxycycline in smooth muscle cells","name":"articletitle","label":"Article Title"},{"value":"Journal of Pharmacological Sciences","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.jphs.2016.03.008","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2016 The Authors. Production and hosting by Elsevier B.V. on behalf of Japanese Pharmacological Society.","name":"copyright","label":"Copyright"}]}}