{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,21]],"date-time":"2026-05-21T19:36:16Z","timestamp":1779392176174,"version":"3.53.1"},"reference-count":16,"publisher":"Elsevier BV","issue":"3","license":[{"start":{"date-parts":[[2022,3,1]],"date-time":"2022-03-01T00:00:00Z","timestamp":1646092800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2022,3,1]],"date-time":"2022-03-01T00:00:00Z","timestamp":1646092800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2022,1,6]],"date-time":"2022-01-06T00:00:00Z","timestamp":1641427200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Journal of Pharmacological Sciences"],"published-print":{"date-parts":[[2022,3]]},"DOI":"10.1016\/j.jphs.2022.01.002","type":"journal-article","created":{"date-parts":[[2022,1,13]],"date-time":"2022-01-13T01:02:29Z","timestamp":1642035749000},"page":"281-285","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":5,"title":["Methotrexate mediates the integrity of intestinal stem cells partly through nitric oxide-dependent Wnt\/\u03b2-catenin signaling in methotrexate-induced rat ileal mucositis"],"prefix":"10.1016","volume":"148","author":[{"given":"Maiko","family":"Machida","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6942-3322","authenticated-orcid":false,"given":"Takuji","family":"Machida","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Masaki","family":"Kikuchi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ayaka","family":"Shimizu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Syunsuke","family":"Ida","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yoshiki","family":"Tawaraya","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Risa","family":"Kato","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Keisuke","family":"Haramaki","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kaori","family":"Yama","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Saki","family":"Shiga","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Masahiko","family":"Hirafuji","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kenji","family":"Iizuka","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.jphs.2022.01.002_bib1","doi-asserted-by":"crossref","first-page":"820","DOI":"10.1002\/cncr.22484","article-title":"Updated clinical practice guidelines for the prevention and treatment of mucositis","volume":"109","author":"Keefe","year":"2007","journal-title":"Cancer"},{"key":"10.1016\/j.jphs.2022.01.002_bib2","first-page":"147","article-title":"Hallmarks of intestinal stem cells","volume":"3","author":"Baulies","year":"2020","journal-title":"Development"},{"key":"10.1016\/j.jphs.2022.01.002_bib3","doi-asserted-by":"crossref","first-page":"4827","DOI":"10.1113\/JP271931","article-title":"The regulatory niche of intestinal stem cells","volume":"594","author":"Sailaja","year":"2016","journal-title":"J\u00a0Physiol"},{"issue":"154","key":"10.1016\/j.jphs.2022.01.002_bib4","doi-asserted-by":"crossref","first-page":"274","DOI":"10.1016\/j.cell.2013.07.004","article-title":"The intestinal crypt, a prototype stem cell compartment","volume":"18","author":"Clevers","year":"2013","journal-title":"Cell"},{"key":"10.1016\/j.jphs.2022.01.002_bib5","doi-asserted-by":"crossref","first-page":"e110675","DOI":"10.1371\/journal.pone.0110675","article-title":"The role of Wnt\/\u03b2-catenin signaling in enterocyte turnover during methotrexate-induced intestinal mucositis in a rat","volume":"9","author":"Sukhotnik","year":"2014","journal-title":"PLoS One"},{"key":"10.1016\/j.jphs.2022.01.002_bib6","doi-asserted-by":"crossref","first-page":"90","DOI":"10.1186\/1471-230X-11-90","article-title":"Role of inducible nitric oxide synthase pathway on methotrexate-induced intestinal mucositis in rodents","volume":"11","author":"Leit\u00e3o","year":"2011","journal-title":"BMC Gastroenterol"},{"key":"10.1016\/j.jphs.2022.01.002_bib7","doi-asserted-by":"crossref","first-page":"30","DOI":"10.1016\/j.jphs.2020.02.006","article-title":"The role of nitric oxide in small intestine differs between a single and a consecutive administration of methotrexate to rats","volume":"143","author":"Shiga","year":"2020","journal-title":"J\u00a0Pharmacol Sci"},{"key":"10.1016\/j.jphs.2022.01.002_bib8","author":"Nusse"},{"key":"10.1016\/j.jphs.2022.01.002_bib9","doi-asserted-by":"crossref","first-page":"48","DOI":"10.1186\/s12935-018-0543-x","article-title":"Effects of secreted frizzled-related protein 1 on proliferation, migration, invasion, and apoptosis of colorectal cancer cells","volume":"18","author":"Wang","year":"2018","journal-title":"Cancer Cell Int"},{"key":"10.1016\/j.jphs.2022.01.002_bib10","doi-asserted-by":"crossref","first-page":"1003","DOI":"10.1038\/nature06196","article-title":"Identification of stem cells in small intestine and colon by marker gene Lgr5","volume":"449","author":"Barker","year":"2007","journal-title":"Nature"},{"key":"10.1016\/j.jphs.2022.01.002_bib11","first-page":"405","article-title":"Dual action of nitric oxide in pathogenesis of indomethacin-induced small intestinal ulceration in rats","volume":"50","author":"Tanaka","year":"1999","journal-title":"J\u00a0Physiol Pharmacol"},{"key":"10.1016\/j.jphs.2022.01.002_bib12","doi-asserted-by":"crossref","first-page":"1251","DOI":"10.1038\/bjc.2016.112","article-title":"Full-length LGR5-positive cells have chemoresistant characteristics in colorectal cancer","volume":"114","author":"Osawa","year":"2016","journal-title":"Br J Cancer"},{"key":"10.1016\/j.jphs.2022.01.002_bib13","doi-asserted-by":"crossref","first-page":"6543","DOI":"10.2174\/1381612820666140826153203","article-title":"Attacking c-Myc: targeted and combined therapies for cancer","volume":"20","author":"Huang","year":"2014","journal-title":"Curr Pharmaceut Des"},{"key":"10.1016\/j.jphs.2022.01.002_bib14","doi-asserted-by":"crossref","first-page":"1733","DOI":"10.1248\/bpb.b19-00464","article-title":"Potentiation of glucagon-like peptide-2 dynamics by methotrexate administration in rat small intestine","volume":"42","author":"Machida","year":"2019","journal-title":"Biol Pharm Bull"},{"issue":"11","key":"10.1016\/j.jphs.2022.01.002_bib15","doi-asserted-by":"crossref","first-page":"811","DOI":"10.3389\/fgene.2020.00811","article-title":"Epigenetic regulation mediated by methylation in the pathogenesis and precision medicine of rheumatoid arthritis","volume":"4","author":"Guo","year":"2020","journal-title":"Front Genet"},{"issue":"118","key":"10.1016\/j.jphs.2022.01.002_bib16","doi-asserted-by":"crossref","first-page":"4969","DOI":"10.1002\/cncr.27519","article-title":"Helicobacter pylori induces promoter methylation of E-cadherin via interleukin-1 activation of nitric oxide production in gastric cancer cells","volume":"15","author":"Huang","year":"2012","journal-title":"Cancer"}],"container-title":["Journal of Pharmacological Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1347861322000020?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1347861322000020?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,10,22]],"date-time":"2025-10-22T20:00:51Z","timestamp":1761163251000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S1347861322000020"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,3]]},"references-count":16,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2022,3]]}},"alternative-id":["S1347861322000020"],"URL":"https:\/\/doi.org\/10.1016\/j.jphs.2022.01.002","relation":{},"ISSN":["1347-8613"],"issn-type":[{"value":"1347-8613","type":"print"}],"subject":[],"published":{"date-parts":[[2022,3]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Methotrexate mediates the integrity of intestinal stem cells partly through nitric oxide-dependent Wnt\/\u03b2-catenin signaling in methotrexate-induced rat ileal mucositis","name":"articletitle","label":"Article Title"},{"value":"Journal of Pharmacological Sciences","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.jphs.2022.01.002","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2022 The Authors. Production and hosting by Elsevier B.V. on behalf of Japanese Pharmacological Society.","name":"copyright","label":"Copyright"}]}}