{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,17]],"date-time":"2026-10-17T15:14:56Z","timestamp":1792250096455,"version":"4.4.0"},"reference-count":114,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2019,4,1]],"date-time":"2019-04-01T00:00:00Z","timestamp":1554076800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2019,4,1]],"date-time":"2019-04-01T00:00:00Z","timestamp":1554076800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"}],"funder":[{"DOI":"10.13039\/100014440","name":"Ministerio de Ciencia, Innovaci\u00f3n y Universidades","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100014440","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000641","name":"Institution of Civil Engineers","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100000641","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003329","name":"Ministerio de Econom\u00eda y Competitividad","doi-asserted-by":"publisher","award":["PCIN-2016-081"],"award-info":[{"award-number":["PCIN-2016-081"]}],"id":[{"id":"10.13039\/501100003329","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100008431","name":"Consejer\u00eda de Educaci\u00f3n, Junta de Castilla y Le\u00f3n","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100008431","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Journal of Proteomics"],"published-print":{"date-parts":[[2019,4]]},"DOI":"10.1016\/j.jprot.2018.11.006","type":"journal-article","created":{"date-parts":[[2018,11,7]],"date-time":"2018-11-07T18:27:19Z","timestamp":1541615239000},"page":"119-131","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":41,"special_numbering":"C","title":["Proteomics and Penicillium chrysogenum: Unveiling the secrets behind penicillin production"],"prefix":"10.1016","volume":"198","author":[{"given":"Carlos","family":"Barreiro","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carlos","family":"Garc\u00eda-Estrada","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.jprot.2018.11.006_bb0005","first-page":"135","article-title":"Penicillin's discovery and antibiotic resistance: Lessons for the future?","volume":"90","author":"Lobanovska","year":"2017","journal-title":"Yale J Biol Med"},{"key":"10.1016\/j.jprot.2018.11.006_bb0010","first-page":"780","article-title":"On the antibacterial action of cultures of a penicillium, with special reference to their use in the isolation of B. influenzae","volume":"79","author":"Fleming","year":"2001","journal-title":"Bull. World Health Organ."},{"key":"10.1016\/j.jprot.2018.11.006_bb0015","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1155\/2012\/105109","article-title":"Proteomics shows new faces for the old penicillin producer Penicillium chrysogenum","volume":"2012","author":"Barreiro","year":"2012","journal-title":"J Biomed Biotechnol"},{"key":"10.1016\/j.jprot.2018.11.006_bb0020","doi-asserted-by":"crossref","first-page":"163","DOI":"10.1016\/S0065-2164(01)49013-7","article-title":"Alexander Fleming and the discovery of penicillin","volume":"49","author":"Bennett","year":"2001","journal-title":"Adv. Appl. Microbiol."},{"key":"10.1016\/j.jprot.2018.11.006_bb0025","doi-asserted-by":"crossref","first-page":"849","DOI":"10.3201\/eid2305.161556","article-title":"The discovery of penicillin\u2014new insights after more than 75 years of clinical use","volume":"23","author":"Gaynes","year":"2017","journal-title":"Emerg. Infect. Dis."},{"key":"10.1016\/j.jprot.2018.11.006_bb0030","doi-asserted-by":"crossref","first-page":"87","DOI":"10.5598\/imafungus.2011.02.01.12","article-title":"Fleming's penicillin producing strain is not Penicillium chrysogenum but P. rubens","volume":"2","author":"Houbraken","year":"2011","journal-title":"IMA Fungus"},{"key":"10.1016\/j.jprot.2018.11.006_bb0035","doi-asserted-by":"crossref","first-page":"366","DOI":"10.11622\/smedj.2015105","article-title":"Alexander Fleming (1881\u20131955): Discoverer of penicillin","volume":"56","author":"Tan","year":"2015","journal-title":"Singap. Med. J."},{"key":"10.1016\/j.jprot.2018.11.006_bb0040","series-title":"The Penicillins: Properties, Biosynthesis, and Fermentation","volume":"Vol. 3","author":"Herschbach","year":"1984"},{"key":"10.1016\/j.jprot.2018.11.006_bb0045","doi-asserted-by":"crossref","first-page":"91","DOI":"10.1002\/star.19750270306","article-title":"Evaluation of various Brands of Corn Steep Liquor for Penicillin Production","volume":"27","author":"El-Marsafy","year":"1975","journal-title":"Starch - St\u00e4rke"},{"key":"10.1016\/j.jprot.2018.11.006_bb0050","doi-asserted-by":"crossref","first-page":"297","DOI":"10.1128\/br.12.4.297-311.1948","article-title":"Corn steep liquor in microbiology","volume":"12","author":"Liggett","year":"1948","journal-title":"Bacteriol. Rev."},{"key":"10.1016\/j.jprot.2018.11.006_bb0055","doi-asserted-by":"crossref","first-page":"617","DOI":"10.1126\/science.102.2659.617","article-title":"The effect of corn steep liquor ash on penicillin production","volume":"102","author":"Knight","year":"1945","journal-title":"Science"},{"key":"10.1016\/j.jprot.2018.11.006_bb0060","first-page":"117","article-title":"Effect of media composition on the penicillin production","volume":"132","author":"El-Marsafy","year":"1977","journal-title":"Zentralbl Bakteriol Parasitenkd Infektionskr Hyg"},{"key":"10.1016\/j.jprot.2018.11.006_bb0065","doi-asserted-by":"crossref","first-page":"208","DOI":"10.1128\/am.1.4.208-211.1953","article-title":"Penicillin production in corn steep media with continuous carbohydrate addition","volume":"1","author":"Davey","year":"1953","journal-title":"Appl. Microbiol."},{"key":"10.1016\/j.jprot.2018.11.006_bb0070","series-title":"Penicillin Production through Deep-Tank Fermentation","first-page":"1","author":"American Chemical Society","year":"2008"},{"key":"10.1016\/j.jprot.2018.11.006_bb0075","author":"Flavell-While","year":"2010","journal-title":"Chem. Eng."},{"key":"10.1016\/j.jprot.2018.11.006_bb0080","doi-asserted-by":"crossref","first-page":"323","DOI":"10.4321\/S0211-95362011000200004","article-title":"Innovators, deep fermentation and antibiotics: Promoting applied science before and after the Second World War","volume":"31","author":"Bud","year":"2011","journal-title":"Dynamis"},{"key":"10.1016\/j.jprot.2018.11.006_bb0085","doi-asserted-by":"crossref","first-page":"1823","DOI":"10.1021\/ie50489a036","article-title":"Penicillin Fermentations in a Waldhof-Type Fermentor","volume":"42","author":"Brown","year":"1950","journal-title":"Ind. Eng. Chem."},{"key":"10.1016\/j.jprot.2018.11.006_bb0090","doi-asserted-by":"crossref","first-page":"39","DOI":"10.1016\/0167-7799(85)90057-5","article-title":"Biosynthesis of \u03b2-lactam antibiotics: Design and construction of overproducing strains","volume":"3","author":"Martin","year":"1985","journal-title":"Trends Biotechnol."},{"key":"10.1016\/j.jprot.2018.11.006_bb0095","doi-asserted-by":"crossref","first-page":"737","DOI":"10.1038\/171737a0","article-title":"Molecular structure of nucleic acids; a structure for deoxyribose nucleic acid","volume":"171","author":"Watson","year":"1953","journal-title":"Nature"},{"key":"10.1016\/j.jprot.2018.11.006_bb0100","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1534\/genetics.109.112144","article-title":"Personal reflections on the origins and emergence of recombinant DNA technology","volume":"184","author":"Berg","year":"2010","journal-title":"Genetics"},{"key":"10.1016\/j.jprot.2018.11.006_bb0105","doi-asserted-by":"crossref","first-page":"15521","DOI":"10.1073\/pnas.1313397110","article-title":"DNA cloning: a personal view after 40 years","volume":"110","author":"Cohen","year":"2013","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"key":"10.1016\/j.jprot.2018.11.006_bb0110","doi-asserted-by":"crossref","first-page":"3240","DOI":"10.1073\/pnas.70.11.3240","article-title":"Construction of Biologically Functional Bacterial Plasmids In Vitro","volume":"70","author":"Cohen","year":"1973","journal-title":"Proc. Natl. Acad. Sci."},{"key":"10.1016\/j.jprot.2018.11.006_bb0115","first-page":"494","article-title":"High-frequency transformation of Penicillium chrysogenum","volume":"5","author":"Cantoral","year":"1987","journal-title":"Bio\/Technology"},{"key":"10.1016\/j.jprot.2018.11.006_bb0120","doi-asserted-by":"crossref","first-page":"639","DOI":"10.1007\/BF00393928","article-title":"Transformation of Penicillium chrysogenum using the Aspergillus nidulans amdS gene as a dominant selective marker","volume":"11","author":"Beri","year":"1987","journal-title":"Curr. Genet."},{"key":"10.1016\/j.jprot.2018.11.006_bb0125","doi-asserted-by":"crossref","first-page":"97","DOI":"10.1016\/0378-1119(87)90479-3","article-title":"Transformation in Penicillium chrysogenum","volume":"51","author":"S\u00e1nchez","year":"1987","journal-title":"Gene"},{"key":"10.1016\/j.jprot.2018.11.006_bb0130","doi-asserted-by":"crossref","first-page":"91","DOI":"10.1007\/BF00332235","article-title":"Cloning, sequence analysis and transcriptional study of the isopenicillin N synthase of Penicillium chrysogenum AS-P-78","volume":"216","author":"Barredo","year":"1989","journal-title":"Mol. Gen. Genet."},{"key":"10.1016\/j.jprot.2018.11.006_bb0135","doi-asserted-by":"crossref","first-page":"291","DOI":"10.1016\/0378-1119(89)90115-7","article-title":"Cloning and characterization of the acyl-coenzyme a: 6-aminopenicillanic-acid-acyltransferase gene of Penicillium chrysogenum","volume":"83","author":"Barredo","year":"1989","journal-title":"Gene"},{"key":"10.1016\/j.jprot.2018.11.006_bb0140","doi-asserted-by":"crossref","first-page":"16358","DOI":"10.1016\/S0021-9258(17)46231-4","article-title":"The cluster of penicillin biosynthetic genes. Identification and characterization of the pcbAB gene encoding the alpha-aminoadipyl-cysteinyl-valine synthetase and linkage to the pcbC and penDE genes","volume":"265","author":"D\u00edez","year":"1990","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.jprot.2018.11.006_bb0145","doi-asserted-by":"crossref","first-page":"12646","DOI":"10.1016\/S0021-9258(18)98948-9","article-title":"Delta-(L-alpha-aminoadipyl)-L-cysteinyl-D-valine synthetase from Aspergillus nidulans. Molecular characterization of the acvA gene encoding the first enzyme of the penicillin biosynthetic pathway","volume":"266","author":"MacCabe","year":"1991","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.jprot.2018.11.006_bb0150","doi-asserted-by":"crossref","first-page":"6200","DOI":"10.1073\/pnas.92.13.6200","article-title":"The penicillin gene cluster is amplified in tandem repeats linked by conserved hexanucleotide sequences","volume":"92","author":"Fierro","year":"1995","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"key":"10.1016\/j.jprot.2018.11.006_bb0155","first-page":"464","article-title":"Why did the Fleming strain fail in penicillin industry ?","volume":"42","author":"Rodr\u00edguez-S\u00e1iz","year":"2005"},{"key":"10.1016\/j.jprot.2018.11.006_bb0160","doi-asserted-by":"crossref","first-page":"5465","DOI":"10.1128\/JB.183.19.5465-5471.2001","article-title":"Reduced function of a phenylacetate-oxidizing cytochrome p450 caused strong genetic improvement in early phylogeny of penicillin-producing strains","volume":"183","author":"Rodr\u00edguez-S\u00e1iz","year":"2001","journal-title":"J. Bacteriol."},{"key":"10.1016\/j.jprot.2018.11.006_bb0165","doi-asserted-by":"crossref","first-page":"21199","DOI":"10.1074\/jbc.270.36.21199","article-title":"Molecular characterization of a gene encoding a homogentisate dioxygenase from Aspergillus nidulans and identification of its human and plant homologues","volume":"270","author":"Fern\u00e1ndez-Ca\u00f1\u00f3n","year":"1995","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.jprot.2018.11.006_bb0170","doi-asserted-by":"crossref","first-page":"14545","DOI":"10.1074\/jbc.274.21.14545","article-title":"Disruption of phacA, an Aspergillus nidulans gene encoding a novel cytochrome P450 monooxygenase catalyzing phenylacetate 2-hydroxylation, results in penicillin overproduction","volume":"274","author":"Mingot","year":"1999","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.jprot.2018.11.006_bb0175","doi-asserted-by":"crossref","first-page":"5062","DOI":"10.1128\/JB.186.15.5062-5077.2004","article-title":"The homogentisate pathway: a central catabolic pathway involved in the degradation of L-phenylalanine, L-tyrosine, and 3-hydroxyphenylacetate in Pseudomonas putida","volume":"186","author":"Arias-Barrau","year":"2004","journal-title":"J. Bacteriol."},{"key":"10.1016\/j.jprot.2018.11.006_bb0180","doi-asserted-by":"crossref","first-page":"514","DOI":"10.1128\/EC.00226-06","article-title":"Novel phacB-encoded cytochrome P450 monooxygenase from Aspergillus nidulans with 3-hydroxyphenylacetate 6-hydroxylase and 3,4-dihydroxyphenylacetate 6-hydroxylase activities","volume":"6","author":"Ferrer-Sevillano","year":"2007","journal-title":"Eukaryot. Cell"},{"key":"10.1016\/j.jprot.2018.11.006_bb0185","doi-asserted-by":"crossref","first-page":"1161","DOI":"10.1038\/nbt.1498","article-title":"Genome sequencing and analysis of the filamentous fungus Penicillium chrysogenum","volume":"26","author":"Van Den Berg","year":"2008","journal-title":"Nat. Biotechnol."},{"issue":"Suppl. 1","key":"10.1016\/j.jprot.2018.11.006_bb0190","doi-asserted-by":"crossref","first-page":"S11","DOI":"10.1186\/1471-2164-15-S1-S11","article-title":"Genome sequencing of high-penicillin producing industrial strain of Penicillium chrysogenum","volume":"15","author":"Wang","year":"2014","journal-title":"BMC Genomics"},{"key":"10.1016\/j.jprot.2018.11.006_bb0195","doi-asserted-by":"crossref","first-page":"214","DOI":"10.1016\/j.biochi.2008.09.004","article-title":"The global regulator LaeA controls penicillin biosynthesis, pigmentation and sporulation, but not roquefortine C synthesis in Penicillium chrysogenum","volume":"91","author":"Kosalkov\u00e1","year":"2009","journal-title":"Biochimie"},{"key":"10.1016\/j.jprot.2018.11.006_bb0200","doi-asserted-by":"crossref","first-page":"1236","DOI":"10.1128\/EC.00077-10","article-title":"Two components of a velvet-like complex control hyphal morphogenesis, conidiophore development, and penicillin biosynthesis in Penicillium chrysogenum","volume":"9","author":"Hoff","year":"2010","journal-title":"Eukaryot. Cell"},{"key":"10.1016\/j.jprot.2018.11.006_bb0205","doi-asserted-by":"crossref","first-page":"972","DOI":"10.1128\/AEM.01557-10","article-title":"Among developmental regulators, StuA but not BrlA is essential for penicillin V production in Penicillium chrysogenum","volume":"77","author":"Sigl","year":"2011","journal-title":"Appl. Environ. Microbiol."},{"key":"10.1016\/j.jprot.2018.11.006_bb0210","doi-asserted-by":"crossref","first-page":"866","DOI":"10.1016\/j.fgb.2012.08.002","article-title":"The regulatory factor PcRFX1 controls the expression of the three genes of \u03b2-lactam biosynthesis in Penicillium chrysogenum","volume":"49","author":"Dom\u00ednguez-Santos","year":"2012","journal-title":"Fungal Genet. Biol."},{"key":"10.1016\/j.jprot.2018.11.006_bb0215","doi-asserted-by":"crossref","first-page":"162","DOI":"10.1016\/j.biochi.2015.05.015","article-title":"PcFKH1, a novel regulatory factor from the forkhead family, controls the biosynthesis of penicillin in Penicillium chrysogenum","volume":"115","author":"Dom\u00ednguez-Santos","year":"2015","journal-title":"Biochimie"},{"key":"10.1016\/j.jprot.2018.11.006_bb0220","doi-asserted-by":"crossref","first-page":"561","DOI":"10.1080\/14786440709463633","article-title":"On rays of positive electricity","volume":"13","author":"Thomson","year":"1907","journal-title":"Phil Mag Ser"},{"key":"10.1016\/j.jprot.2018.11.006_bb0225","doi-asserted-by":"crossref","first-page":"633","DOI":"10.1038\/nmeth.1659","article-title":"A century of mass spectrometry: from atoms to proteomes","volume":"8","author":"Yates","year":"2011","journal-title":"Nat. Methods"},{"key":"10.1016\/j.jprot.2018.11.006_bb0230","first-page":"636","article-title":"Advanced characterization of petroleum crude and products by high field Fourier Transform ion cyclotron resonance mass spectrometry","volume":"47","author":"Rodgers","year":"2002","journal-title":"Fuel Chem Div Prepr"},{"key":"10.1016\/j.jprot.2018.11.006_bb0235","doi-asserted-by":"crossref","first-page":"2240","DOI":"10.1063\/1.1670391","article-title":"Molecular beams of macroions","volume":"49","author":"Dole","year":"1968","journal-title":"J. Chem. Phys."},{"key":"10.1016\/j.jprot.2018.11.006_bb0240","doi-asserted-by":"crossref","first-page":"2274","DOI":"10.1021\/ja00475a072","article-title":"Selected ion fragmentation with a tandem quadrupole mass spectrometer","volume":"100","author":"Yost","year":"1978","journal-title":"J. Am. Chem. Soc."},{"key":"10.1016\/j.jprot.2018.11.006_bb0245","doi-asserted-by":"crossref","first-page":"270","DOI":"10.1038\/293270a0","article-title":"Fast Atom Bombardment of Solids as an Ion Source in Mass Spectrometry","volume":"293","author":"Barber","year":"1981","journal-title":"Nature"},{"key":"10.1016\/j.jprot.2018.11.006_bb0250","doi-asserted-by":"crossref","first-page":"85","DOI":"10.1016\/0168-1176(84)80077-4","article-title":"Recent improvements in and analytical applications of advanced ion trap technology","volume":"60","author":"Stafford","year":"1984","journal-title":"Int. J. Mass Spectrom. Ion Process."},{"key":"10.1016\/j.jprot.2018.11.006_bb0255","doi-asserted-by":"crossref","first-page":"2299","DOI":"10.1021\/ac00171a028","article-title":"Laser desorption ionization of proteins with molecular masses exceeding 10,000 daltons","volume":"60","author":"Karas","year":"1988","journal-title":"Anal. Chem."},{"key":"10.1016\/j.jprot.2018.11.006_bb0260","series-title":"New Trends Corynebacterium glutamicum beyond Amin. Acids","first-page":"45","article-title":"Methods in proteomics applied to Corynebacterium glutamicum","author":"Barreiro","year":"2015"},{"key":"10.1016\/j.jprot.2018.11.006_bb0265","doi-asserted-by":"crossref","first-page":"279","DOI":"10.1017\/S0033583597003399","article-title":"Protein identification in the post-genome era: the rapid rise of proteomics","volume":"30","author":"James","year":"1997","journal-title":"Q. Rev. Biophys."},{"key":"10.1016\/j.jprot.2018.11.006_bb0270","doi-asserted-by":"crossref","first-page":"4842","DOI":"10.1002\/pmic.200800719","article-title":"The pre-omics era: the early days of two-dimensional gels","volume":"8","author":"O'Farrell","year":"2008","journal-title":"Proteomics"},{"key":"10.1016\/j.jprot.2018.11.006_bb0275","doi-asserted-by":"crossref","first-page":"1829","DOI":"10.1016\/j.jprot.2011.05.040","article-title":"Two-dimensional gel electrophoresis in proteomics: a tutorial","volume":"74","author":"Rabilloud","year":"2011","journal-title":"J. Proteome"},{"key":"10.1016\/j.jprot.2018.11.006_bb0280","doi-asserted-by":"crossref","first-page":"680","DOI":"10.1038\/227680a0","article-title":"Cleavage of structural proteins during the assembly of the head of bacteriophage T4","volume":"227","author":"Laemmli","year":"1970","journal-title":"Nature"},{"key":"10.1016\/j.jprot.2018.11.006_bb0285","doi-asserted-by":"crossref","first-page":"4007","DOI":"10.1016\/S0021-9258(19)41496-8","article-title":"High resolution two-dimensional electrophoresis of proteins","volume":"250","author":"O'Farrell","year":"1975","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.jprot.2018.11.006_bb0290","doi-asserted-by":"crossref","first-page":"411","DOI":"10.1002\/9780470939932.ch17","article-title":"Two-dimensional electrophoresis in proteomics","volume":"54","author":"Westermeier","year":"2011","journal-title":"Methods Biochem. Anal."},{"key":"10.1016\/j.jprot.2018.11.006_bb0295","doi-asserted-by":"crossref","first-page":"3665","DOI":"10.1002\/pmic.200401031","article-title":"Current two-dimensional electrophoresis technology for proteomics","volume":"4","author":"G\u00f6rg","year":"2004","journal-title":"Proteomics"},{"key":"10.1016\/j.jprot.2018.11.006_bb0300","doi-asserted-by":"crossref","first-page":"1327","DOI":"10.1002\/elps.200305844","article-title":"Blue silver: a very sensitive colloidal Coomassie G-250 staining for proteome analysis","volume":"25","author":"Candiano","year":"2004","journal-title":"Electrophoresis"},{"key":"10.1016\/j.jprot.2018.11.006_bb0305","doi-asserted-by":"crossref","first-page":"601","DOI":"10.1002\/(SICI)1522-2683(19990301)20:3<601::AID-ELPS601>3.0.CO;2-6","article-title":"Mass spectrometric identification of proteins from silver-stained polyacrylamide gel: a method for the removal of silver ions to enhance sensitivity","volume":"20","author":"Gharahdaghi","year":"1999","journal-title":"Electrophoresis"},{"key":"10.1016\/j.jprot.2018.11.006_bb0310","doi-asserted-by":"crossref","first-page":"1359","DOI":"10.1002\/1615-9861(200111)1:11<1359::AID-PROT1359>3.0.CO;2-Q","article-title":"Improved silver staining protocols for high sensitivity protein identification using matrix-assisted laser desorption\/ionization-time of flight analysis","volume":"1","author":"Mortz","year":"2001","journal-title":"Proteomics"},{"key":"10.1016\/j.jprot.2018.11.006_bb0315","doi-asserted-by":"crossref","first-page":"2071","DOI":"10.1002\/elps.1150181133","article-title":"Difference gel electrophoresis: a single gel method for detecting changes in protein extracts","volume":"18","author":"Unl\u00fc","year":"1997","journal-title":"Electrophoresis"},{"key":"10.1016\/j.jprot.2018.11.006_bb0320","doi-asserted-by":"crossref","first-page":"4886","DOI":"10.1002\/pmic.200800298","article-title":"Difference gel electrophoresis","volume":"8","author":"Timms","year":"2008","journal-title":"Proteomics"},{"key":"10.1016\/j.jprot.2018.11.006_bb0325","doi-asserted-by":"crossref","first-page":"1300","DOI":"10.1021\/ac026136s","article-title":"Fast-response proteomics by accelerated in-gel digestion of proteins","volume":"75","author":"Havlis","year":"2003","journal-title":"Anal. Chem."},{"key":"10.1016\/j.jprot.2018.11.006_bb0330","doi-asserted-by":"crossref","first-page":"568","DOI":"10.1002\/bit.10441","article-title":"Inverse metabolic engineering: a strategy for directed genetic engineering of useful phenotypes","volume":"79","author":"Bailey","year":"2002","journal-title":"Biotechnol. Bioeng."},{"key":"10.1016\/j.jprot.2018.11.006_bb0335","series-title":"Biosynth. Molelcular Genet. Fungal Second. Metab","first-page":"17","article-title":"Penicillins","volume":"vol I","author":"Garc\u00eda-Estrada","year":"2014"},{"key":"10.1016\/j.jprot.2018.11.006_bb0340","doi-asserted-by":"crossref","first-page":"317","DOI":"10.1042\/BJ20080369","article-title":"Post-translational enzyme modification by the phosphopantetheinyl transferase is required for lysine and penicillin biosynthesis but not for roquefortine or fatty acid formation in Penicillium chrysogenum","volume":"415","author":"Garc\u00eda-Estrada","year":"2008","journal-title":"Biochem. J."},{"key":"10.1016\/j.jprot.2018.11.006_bb0345","doi-asserted-by":"crossref","first-page":"973","DOI":"10.1128\/jb.176.4.973-984.1994","article-title":"The thioredoxin system of Penicillium chrysogenum and its possible role in penicillin biosynthesis","volume":"176","author":"Cohen","year":"1994","journal-title":"J. Bacteriol."},{"key":"10.1016\/j.jprot.2018.11.006_bb0350","doi-asserted-by":"crossref","first-page":"147","DOI":"10.1042\/BJ20051599","article-title":"Amplification and disruption of the phenylacetyl-CoA ligase gene of Penicillium chrysogenum encoding an aryl-capping enzyme that supplies phenylacetic acid to the isopenicillin N-acyltransferase","volume":"395","author":"Lamas-Maceiras","year":"2006","journal-title":"Biochem. J."},{"key":"10.1016\/j.jprot.2018.11.006_bb0355","doi-asserted-by":"crossref","first-page":"3567","DOI":"10.1099\/mic.0.2008\/019091-0","article-title":"The heterotrimeric Galpha protein pga1 regulates biosynthesis of penicillin, chrysogenin and roquefortine in Penicillium chrysogenum","volume":"154","author":"Garc\u00eda-Rico","year":"2008","journal-title":"Microbiology"},{"key":"10.1016\/j.jprot.2018.11.006_bb0360","first-page":"45","article-title":"Regulation of penicillin biosynthesis in filamentous fungi","volume":"88","author":"Brakhage","year":"2004","journal-title":"Adv. Biochem. Eng. Biotechnol."},{"key":"10.1016\/j.jprot.2018.11.006_bb0365","doi-asserted-by":"crossref","first-page":"285","DOI":"10.1111\/j.1751-7915.2009.00123.x","article-title":"Regulation and compartmentalization of \u03b2-lactam biosynthesis","volume":"3","author":"Mart\u00edn","year":"2010","journal-title":"Microb. Biotechnol."},{"key":"10.1016\/j.jprot.2018.11.006_bb0370","doi-asserted-by":"crossref","first-page":"47","DOI":"10.3390\/fermentation4020047","article-title":"Transcription Factors Controlling primary and secondary Metabolism in Filamentous Fungi: the \u03b2-Lactam Paradigm","volume":"4","author":"Garc\u00eda-Estrada","year":"2018","journal-title":"Fermentation"},{"key":"10.1016\/j.jprot.2018.11.006_bb0375","doi-asserted-by":"crossref","first-page":"167","DOI":"10.1007\/s10142-009-0110-6","article-title":"Matching the proteome to the genome: the microbody of penicillin-producing Penicillium chrysogenum cells","volume":"9","author":"Kiel","year":"2009","journal-title":"Funct Integr Genomics"},{"key":"10.1016\/j.jprot.2018.11.006_bb0380","doi-asserted-by":"crossref","first-page":"1182","DOI":"10.1074\/mcp.M900327-MCP200","article-title":"Proteome analysis of the penicillin producer Penicillium chrysogenum: characterization of protein changes during the industrial strain improvement","volume":"9","author":"Jami","year":"2010","journal-title":"Mol Cell Proteomics MCP"},{"key":"10.1016\/j.jprot.2018.11.006_bb0385","doi-asserted-by":"crossref","first-page":"27","DOI":"10.1093\/nar\/28.1.27","article-title":"KEGG: Kyoto encyclopedia of genes and genomes","volume":"28","author":"Kanehisa","year":"2000","journal-title":"Nucleic Acids Res."},{"key":"10.1016\/j.jprot.2018.11.006_bb0390","doi-asserted-by":"crossref","first-page":"5539","DOI":"10.1093\/nar\/gkh894","article-title":"The FunCat, a functional annotation scheme for systematic classification of proteins from whole genomes","volume":"32","author":"Ruepp","year":"2004","journal-title":"Nucleic Acids Res."},{"key":"10.1016\/j.jprot.2018.11.006_bb0395","doi-asserted-by":"crossref","first-page":"2729","DOI":"10.1074\/mcp.M110.001412","article-title":"The Penicillium chrysogenum extracellular proteome. Conversion from a food-rotting strain to a versatile cell factory for white biotechnology","volume":"9","author":"Jami","year":"2010","journal-title":"Mol. Cell. Proteomics"},{"key":"10.1016\/j.jprot.2018.11.006_bb0400","doi-asserted-by":"crossref","first-page":"783","DOI":"10.1016\/j.jmb.2004.05.028","article-title":"Improved prediction of signal peptides: SignalP 3.0","volume":"340","author":"Bendtsen","year":"2004","journal-title":"J. Mol. Biol."},{"key":"10.1016\/j.jprot.2018.11.006_bb0405","doi-asserted-by":"crossref","first-page":"349","DOI":"10.1093\/protein\/gzh037","article-title":"Feature-based prediction of non-classical and leaderless protein secretion","volume":"17","author":"Bendtsen","year":"2004","journal-title":"Protein Eng Des Sel"},{"key":"10.1016\/j.jprot.2018.11.006_bb0410","doi-asserted-by":"crossref","first-page":"429","DOI":"10.1080\/00275514.1955.12024468","article-title":"The production and selection of a Family of Strains in Penicillium chrysogenum","volume":"47","author":"Backus","year":"1955","journal-title":"Mycologia"},{"key":"10.1016\/j.jprot.2018.11.006_bb0415","series-title":"Antibiot. Handb. Exp. Pharmacol","doi-asserted-by":"crossref","first-page":"97","DOI":"10.1007\/978-3-642-81966-7_3","article-title":"Strain improvement and preservation of \u03b2-Lactam-producing microorganisms","author":"Elander","year":"1983"},{"key":"10.1016\/j.jprot.2018.11.006_bb0420","doi-asserted-by":"crossref","first-page":"10","DOI":"10.1016\/j.ymben.2007.07.001","article-title":"A metabolome study of the steady-state relation between central metabolism, amino acid biosynthesis and penicillin production in Penicillium chrysogenum","volume":"10","author":"Nasution","year":"2008","journal-title":"Metab. Eng."},{"key":"10.1016\/j.jprot.2018.11.006_bb0425","doi-asserted-by":"crossref","first-page":"618","DOI":"10.1016\/j.fgb.2006.03.001","article-title":"Transcriptional and bioinformatic analysis of the 56.8 kb DNA region amplified in tandem repeats containing the penicillin gene cluster in Penicillium chrysogenum","volume":"43","author":"Fierro","year":"2006","journal-title":"Fungal Genet. Biol."},{"key":"10.1016\/j.jprot.2018.11.006_bb0430","doi-asserted-by":"crossref","first-page":"18","DOI":"10.1038\/sj.jim.2900411","article-title":"Analysis of a commercially improved Penicillium chrysogenum strain series: involvement of recombinogenic regions in amplification and deletion of the penicillin biosynthesis gene cluster","volume":"19","author":"Newbert","year":"1997","journal-title":"J. Ind. Microbiol. Biotechnol."},{"key":"10.1016\/j.jprot.2018.11.006_bb0435","doi-asserted-by":"crossref","first-page":"788","DOI":"10.1007\/s12010-016-2031-x","article-title":"Comprehensive profiling of proteome changes provide insights of industrial Penicillium chrysogenum during pilot and industrial penicillin G fermentation","volume":"179","author":"Cheng","year":"2016","journal-title":"Appl. Biochem. Biotechnol."},{"key":"10.1016\/j.jprot.2018.11.006_bb0440","doi-asserted-by":"crossref","first-page":"1223","DOI":"10.1007\/s12010-012-9852-z","article-title":"Comparative metabolomic study of Penicillium chrysogenum during pilot and industrial penicillin fermentations","volume":"168","author":"Ding","year":"2012","journal-title":"Appl. Biochem. Biotechnol."},{"key":"10.1016\/j.jprot.2018.11.006_bb0445","doi-asserted-by":"crossref","first-page":"285","DOI":"10.1111\/j.1364-3703.2006.00338.x","article-title":"Signalling pathways connecting mycotoxin production and sporulation","volume":"7","author":"Brodhagen","year":"2006","journal-title":"Mol. Plant Pathol."},{"key":"10.1016\/j.jprot.2018.11.006_bb0450","doi-asserted-by":"crossref","first-page":"437","DOI":"10.1016\/j.resmic.2007.03.001","article-title":"The pga1 gene of Penicillium chrysogenum NRRL 1951 encodes a heterotrimeric G protein alpha subunit that controls growth and development","volume":"158","author":"Garc\u00eda-Rico","year":"2007","journal-title":"Res. Microbiol."},{"key":"10.1016\/j.jprot.2018.11.006_bb0455","doi-asserted-by":"crossref","first-page":"57","DOI":"10.1139\/O07-148","article-title":"Heterotrimeric Galpha protein Pga1 of Penicillium chrysogenum controls conidiation mainly by a cAMP-independent mechanism","volume":"86","author":"Garc\u00eda-Rico","year":"2008","journal-title":"Biochem. Cell Biol."},{"key":"10.1016\/j.jprot.2018.11.006_bb0460","doi-asserted-by":"crossref","first-page":"641","DOI":"10.1016\/j.fgb.2010.11.013","article-title":"Heterotrimeric G\u03b1 protein Pga1 from Penicillium chrysogenum triggers germination in response to carbon sources and affects negatively resistance to different stress conditions","volume":"48","author":"Garc\u00eda-Rico","year":"2011","journal-title":"Fungal Genet. Biol."},{"key":"10.1016\/j.jprot.2018.11.006_bb0465","doi-asserted-by":"crossref","first-page":"173","DOI":"10.1186\/s12934-016-0564-x","article-title":"Proteomic analysis of the signaling pathway mediated by the heterotrimeric G\u03b1 protein Pga1 of Penicillium chrysogenum","volume":"15","author":"Carrasco-Navarro","year":"2016","journal-title":"Microb. Cell Factories"},{"key":"10.1016\/j.jprot.2018.11.006_bb0470","doi-asserted-by":"crossref","first-page":"129","DOI":"10.1016\/j.jprot.2013.04.028","article-title":"The inducers 1,3-diaminopropane and spermidine cause the reprogramming of metabolism in Penicillium chrysogenum, leading to multiple vesicles and penicillin overproduction","volume":"85","author":"Garc\u00eda-Estrada","year":"2013","journal-title":"J. Proteome"},{"key":"10.1016\/j.jprot.2018.11.006_bb0475","doi-asserted-by":"crossref","first-page":"52","DOI":"10.1016\/j.jprot.2016.12.021","article-title":"Casein phosphopeptides and CaCl2 increase penicillin production and cause an increment in microbody\/peroxisome proteins in Penicillium chrysogenum","volume":"156","author":"Dom\u00ednguez-Santos","year":"2017","journal-title":"J. Proteome"},{"key":"10.1016\/j.jprot.2018.11.006_bb0480","doi-asserted-by":"crossref","first-page":"243","DOI":"10.1016\/j.jprot.2018.08.006","article-title":"Catabolism of phenylacetic acid in Penicillium rubens. Proteome-wide analysis in response to the benzylpenicillin side chain precursor","volume":"187","author":"Jami","year":"2018","journal-title":"J. Proteome"},{"key":"10.1016\/j.jprot.2018.11.006_bb0485","doi-asserted-by":"crossref","first-page":"5688","DOI":"10.1128\/AEM.00059-11","article-title":"Characterization of an autoinducer of penicillin biosynthesis in Penicillium chrysogenum","volume":"77","author":"Mart\u00edn","year":"2011","journal-title":"Appl. Environ. Microbiol."},{"key":"10.1016\/j.jprot.2018.11.006_bb0490","doi-asserted-by":"crossref","first-page":"1004","DOI":"10.1016\/j.fgb.2012.10.001","article-title":"The inducers 1,3-diaminopropane and spermidine produce a drastic increase in the expression of the penicillin biosynthetic genes for prolonged time, mediated by the laeA regulator","volume":"49","author":"Mart\u00edn","year":"2012","journal-title":"Fungal Genet. Biol."},{"key":"10.1016\/j.jprot.2018.11.006_bb0495","doi-asserted-by":"crossref","first-page":"241","DOI":"10.7164\/antibiotics.44.241","article-title":"Isolation and partial characterisation of ACV synthetase from Cephalosporium acremonium and Streptomyces clavuligerus. Evidence for the presence of phosphopantothenate in ACV synthetase","volume":"44","author":"Baldwin","year":"1991","journal-title":"J Antibiot (Tokyo)"},{"key":"10.1016\/j.jprot.2018.11.006_bb0500","doi-asserted-by":"crossref","first-page":"35","DOI":"10.1016\/j.fgb.2010.05.006","article-title":"Compartmentalization and molecular traffic in secondary metabolism: a new understanding of established cellular processes","volume":"48","author":"Roze","year":"2011","journal-title":"Fungal Genet. Biol."},{"key":"10.1016\/j.jprot.2018.11.006_bb0505","doi-asserted-by":"crossref","first-page":"197","DOI":"10.1515\/bmc-2012-0048","article-title":"Transport of substrates into peroxisomes: the paradigm of \u03b2-lactam biosynthetic intermediates","volume":"4","author":"Mart\u00edn","year":"2013","journal-title":"Biomol Concepts"},{"key":"10.1016\/j.jprot.2018.11.006_bb0510","doi-asserted-by":"crossref","first-page":"5","DOI":"10.1186\/1475-2859-11-5","article-title":"Casein phosphopeptides drastically increase the secretion of extracellular proteins in Aspergillus awamori. Proteomics studies reveal changes in the secretory pathway","volume":"11","author":"Kosalkov\u00e1","year":"2012","journal-title":"Microb. Cell Factories"},{"key":"10.1016\/j.jprot.2018.11.006_bb0515","doi-asserted-by":"crossref","first-page":"117","DOI":"10.1186\/s12934-014-0117-0","article-title":"Calcium-containing phosphopeptides pave the secretory pathway for efficient protein traffic and secretion in fungi","volume":"13","author":"Mart\u00edn","year":"2014","journal-title":"Microb. Cell Factories"},{"key":"10.1016\/j.jprot.2018.11.006_bb0520","doi-asserted-by":"crossref","first-page":"541","DOI":"10.1111\/j.1365-2958.2004.03994.x","article-title":"Blockage of methylcitrate cycle inhibits polyketide production in Aspergillus nidulans","volume":"52","author":"Zhang","year":"2004","journal-title":"Mol. Microbiol."},{"key":"10.1016\/j.jprot.2018.11.006_bb0525","doi-asserted-by":"crossref","first-page":"71","DOI":"10.1016\/0378-1097(89)90368-6","article-title":"Regulation of delta-(L-alpha-aminoadipyl)-L-cysteinyl-D-valine and isopenicillin N biosynthesis in Penicillium chrysogenum by the alpha-aminoadipate pool size","volume":"53","author":"H\u00f6nlinger","year":"1989","journal-title":"FEMS Microbiol. Lett."},{"key":"10.1016\/j.jprot.2018.11.006_bb0530","doi-asserted-by":"crossref","first-page":"172","DOI":"10.1016\/S0006-291X(82)80027-2","article-title":"Induction of penicillin biosynthesis by L-glutamate in Penicillium chrysogenum","volume":"105","author":"Lara","year":"1982","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"10.1016\/j.jprot.2018.11.006_bb0535","doi-asserted-by":"crossref","first-page":"329","DOI":"10.1128\/jb.53.3.329-341.1947","article-title":"Penicillin: X. the effect of Phenylacetic Acid on Penicillin Production","volume":"53","author":"Moyer","year":"1947","journal-title":"J. Bacteriol."},{"key":"10.1016\/j.jprot.2018.11.006_bb0540","doi-asserted-by":"crossref","first-page":"443","DOI":"10.1016\/j.fgb.2012.04.003","article-title":"Fungal S-adenosylmethionine synthetase and the control of development and secondary metabolism in Aspergillus nidulans","volume":"49","author":"Gerke","year":"2012","journal-title":"Fungal Genet. Biol."},{"key":"10.1016\/j.jprot.2018.11.006_bb0545","doi-asserted-by":"crossref","first-page":"1242","DOI":"10.1099\/mic.0.2007\/010017-0","article-title":"NADPH-dependent glutamate dehydrogenase in Penicillium chrysogenum is involved in regulation of beta-lactam production","volume":"154","author":"Thykaer","year":"2008","journal-title":"Microbiology"},{"key":"10.1016\/j.jprot.2018.11.006_bb0550","doi-asserted-by":"crossref","first-page":"1087","DOI":"10.1038\/ja.2017.124","article-title":"Incentivising innovation in antibiotic drug discovery and development: Progress, challenges and next steps","volume":"70","author":"Simpkin","year":"2017","journal-title":"J Antibiot (Tokyo)"},{"issue":"3","key":"10.1016\/j.jprot.2018.11.006_bb0555","first-page":"1","article-title":"Microbial metabolites in nutrition, healthcare and agriculture","volume":"7","author":"Singh","year":"2017","journal-title":"Biotech"},{"key":"10.1016\/j.jprot.2018.11.006_bb0560","doi-asserted-by":"crossref","first-page":"386","DOI":"10.3181\/00379727-51-13986","article-title":"Resistance of Staphylococcus aureus to the Action of Penicillin","volume":"51","author":"Rammelkamp","year":"1942","journal-title":"Exp. Biol. Med."},{"key":"10.1016\/j.jprot.2018.11.006_bb0565","series-title":"Antimicrob. Compd","first-page":"285","article-title":"Bacterial Cell Walls","author":"Santos-Beneit","year":"2014"},{"key":"10.1016\/j.jprot.2018.11.006_bb0570","doi-asserted-by":"crossref","first-page":"473","DOI":"10.1038\/nrc2394","article-title":"Paul Ehrlich \u2019 s magic bullet concept : 100 years of progress","volume":"8","author":"Strebhardt","year":"2008","journal-title":"Nat. Rev. Cancer"}],"container-title":["Journal of Proteomics"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1874391918303968?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1874391918303968?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,10,29]],"date-time":"2025-10-29T12:01:10Z","timestamp":1761739270000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S1874391918303968"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,4]]},"references-count":114,"alternative-id":["S1874391918303968"],"URL":"https:\/\/doi.org\/10.1016\/j.jprot.2018.11.006","relation":{},"ISSN":["1874-3919"],"issn-type":[{"value":"1874-3919","type":"print"}],"subject":[],"published":{"date-parts":[[2019,4]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Proteomics and Penicillium chrysogenum: Unveiling the secrets behind penicillin production","name":"articletitle","label":"Article Title"},{"value":"Journal of Proteomics","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.jprot.2018.11.006","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2018 Elsevier B.V. All rights reserved.","name":"copyright","label":"Copyright"}]}}