{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,17]],"date-time":"2026-10-17T18:28:20Z","timestamp":1792261700712,"version":"4.4.0"},"reference-count":37,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2026,6,1]],"date-time":"2026-06-01T00:00:00Z","timestamp":1780272000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2026,6,1]],"date-time":"2026-06-01T00:00:00Z","timestamp":1780272000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2026,5,11]],"date-time":"2026-05-11T00:00:00Z","timestamp":1778457600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003329","name":"Ministerio de Econom\u00eda y Competitividad","doi-asserted-by":"publisher","award":["PID2023-152778OB-I00"],"award-info":[{"award-number":["PID2023-152778OB-I00"]}],"id":[{"id":"10.13039\/501100003329","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003329","name":"Ministerio de Econom\u00eda y Competitividad","doi-asserted-by":"publisher","award":["MICIU\/AEI\/10.13039\/501100011033"],"award-info":[{"award-number":["MICIU\/AEI\/10.13039\/501100011033"]}],"id":[{"id":"10.13039\/501100003329","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100014440","name":"Gobierno de Espana Ministerio de Ciencia e Innovacion","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100014440","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004587","name":"Carlos III Health Institute","doi-asserted-by":"publisher","award":["FISS-RD24\/0007\/0009"],"award-info":[{"award-number":["FISS-RD24\/0007\/0009"]}],"id":[{"id":"10.13039\/501100004587","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100010585","name":"Basque Government Department of Health","doi-asserted-by":"publisher","award":["2025333005"],"award-info":[{"award-number":["2025333005"]}],"id":[{"id":"10.13039\/501100010585","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Journal of Proteomics"],"published-print":{"date-parts":[[2026,6]]},"DOI":"10.1016\/j.jprot.2026.105678","type":"journal-article","created":{"date-parts":[[2026,5,14]],"date-time":"2026-05-14T12:22:15Z","timestamp":1778761335000},"page":"105678","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":3,"special_numbering":"C","title":["Standardizing tear proteomics preanalytics: Capillary vs Schirmer sampling and denaturing vs mild elution buffers shape proteome depth and abundance profiles"],"prefix":"10.1016","volume":"329","author":[{"given":"Isabel","family":"Cerro-Pardo","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amaiur","family":"Mendizabal","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mikel","family":"Azkargorta","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"F\u00e9lix","family":"Elortza","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"I\u00f1aki","family":"Rodr\u00edguez-Agirretxe","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Arantxa","family":"Acera","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"issue":"5","key":"10.1016\/j.jprot.2026.105678_bb0005","first-page":"71","article-title":"The pathophysiology, diagnosis, and treatment of dry eye disease","volume":"112","author":"Messmer","year":"2015","journal-title":"Dtsch. Arztebl. Int."},{"issue":"8","key":"10.1016\/j.jprot.2026.105678_bb0010","doi-asserted-by":"crossref","first-page":"762","DOI":"10.1097\/01.ico.0000133997.07144.9e","article-title":"The lack of association between signs and symptoms in patients with dry eye disease","volume":"23","author":"Nichols","year":"2004","journal-title":"Cornea"},{"issue":"6","key":"10.1016\/j.jprot.2026.105678_bb0015","doi-asserted-by":"crossref","first-page":"Bio1","DOI":"10.1167\/iovs.17-21709","article-title":"The growing need for validated biomarkers and endpoints for dry eye clinical research","volume":"58","author":"Roy","year":"2017","journal-title":"Invest. Ophthalmol. Vis. Sci."},{"key":"10.1016\/j.jprot.2026.105678_bb0020","doi-asserted-by":"crossref","DOI":"10.1016\/j.exer.2022.109121","article-title":"Tear and ocular surface disease biomarkers: a diagnostic and clinical perspective for ocular allergies and dry eye disease","volume":"221","author":"Su\u00e1rez-Cort\u00e9s","year":"2022","journal-title":"Exp. Eye Res."},{"issue":"1","key":"10.1016\/j.jprot.2026.105678_bb0025","doi-asserted-by":"crossref","first-page":"15","DOI":"10.1186\/s13167-016-0065-3","article-title":"Tear fluid biomarkers in ocular and systemic disease: potential use for predictive, preventive and personalised medicine","volume":"7","author":"Hagan","year":"2016","journal-title":"EPMA J."},{"key":"10.1016\/j.jprot.2026.105678_bb0030","doi-asserted-by":"crossref","first-page":"359","DOI":"10.1016\/j.jprot.2016.05.006","article-title":"Human tear proteomics and peptidomics in ophthalmology: toward the translation of proteomic biomarkers into clinical practice","volume":"150","author":"Azkargorta","year":"2017","journal-title":"J. Proteome"},{"key":"10.1016\/j.jprot.2026.105678_bb0035","doi-asserted-by":"crossref","DOI":"10.1016\/j.exer.2022.109101","article-title":"Biochemistry of human tear film: a review","volume":"220","author":"Masoudi","year":"2022","journal-title":"Exp. Eye Res."},{"key":"10.1016\/j.jprot.2026.105678_bb0040","series-title":"The tearing patient: diagnosis and management","author":"Price M","year":"2009"},{"issue":"4","key":"10.1016\/j.jprot.2026.105678_bb0045","doi-asserted-by":"crossref","DOI":"10.3390\/ijms23042307","article-title":"Comparison of different mass spectrometry workflows for the proteomic analysis of tear fluid","volume":"23","author":"Jones","year":"2022","journal-title":"Int. J. Mol. Sci."},{"issue":"5","key":"10.1016\/j.jprot.2026.105678_bb0050","doi-asserted-by":"crossref","first-page":"842","DOI":"10.1002\/mas.21691","article-title":"Mass spectrometry-based tear proteomics for noninvasive biomarker discovery","volume":"41","author":"Ponzini","year":"2022","journal-title":"Mass Spectrom. Rev."},{"issue":"1","key":"10.1016\/j.jprot.2026.105678_bb0055","doi-asserted-by":"crossref","DOI":"10.1038\/s41598-023-28363-z","article-title":"Pre-analytical sample handling effects on tear fluid protein levels","volume":"13","author":"Gijs","year":"2023","journal-title":"Sci. Rep."},{"issue":"3","key":"10.1016\/j.jprot.2026.105678_bb0060","doi-asserted-by":"crossref","first-page":"16","DOI":"10.1167\/tvst.9.3.16","article-title":"Comparison of capillary and Schirmer strip tear fluid sampling methods using SWATH-MS proteomics approach","volume":"9","author":"N\u00e4ttinen","year":"2020","journal-title":"Transl. Vis. Sci. Technol."},{"issue":"1","key":"10.1016\/j.jprot.2026.105678_bb0065","doi-asserted-by":"crossref","DOI":"10.1038\/s41598-023-31227-1","article-title":"Evaluation of pre-processing methods for tear fluid proteomics using proximity extension assays","volume":"13","author":"Vergouwen","year":"2023","journal-title":"Sci. Rep."},{"issue":"13","key":"10.1016\/j.jprot.2026.105678_bb0070","doi-asserted-by":"crossref","first-page":"8327","DOI":"10.1167\/iovs.13-12132","article-title":"Tear cytokine profile as a noninvasive biomarker of inflammation for ocular surface diseases: standard operating procedures","volume":"54","author":"Wei","year":"2013","journal-title":"Invest. Ophthalmol. Vis. Sci."},{"issue":"5","key":"10.1016\/j.jprot.2026.105678_bb0075","doi-asserted-by":"crossref","DOI":"10.3892\/ijmm.2021.4916","article-title":"Critical role of mass spectrometry proteomics in tear biomarker discovery for multifactorial ocular diseases (review)","volume":"47","author":"Ma","year":"2021","journal-title":"Int. J. Mol. Med."},{"issue":"5","key":"10.1016\/j.jprot.2026.105678_bb0080","doi-asserted-by":"crossref","first-page":"486","DOI":"10.1111\/aos.15059","article-title":"Looking deeper into ocular surface health: an introduction to clinical tear proteomics analysis","volume":"100","author":"N\u00e4ttinen","year":"2022","journal-title":"Acta Ophthalmol."},{"issue":"20","key":"10.1016\/j.jprot.2026.105678_bb0085","doi-asserted-by":"crossref","DOI":"10.3390\/ijerph192013341","article-title":"Changes in tear proteomic profile in ocular diseases","volume":"19","author":"Winiarczyk","year":"2022","journal-title":"Int. J. Environ. Res. Public Health"},{"issue":"7","key":"10.1016\/j.jprot.2026.105678_bb0090","doi-asserted-by":"crossref","first-page":"305","DOI":"10.1080\/14737159.2025.2486680","article-title":"The status of standardization in methodologies for tear fluid biomarkers","volume":"25","author":"Gijs","year":"2025","journal-title":"Expert. Rev. Mol. Diagn."},{"issue":"21","key":"10.1016\/j.jprot.2026.105678_bb0095","doi-asserted-by":"crossref","first-page":"5088","DOI":"10.1039\/c2an35821b","article-title":"Post-collection processing of Schirmer strip-collected human tear fluid impacts protein content","volume":"137","author":"Denisin","year":"2012","journal-title":"Analyst"},{"key":"10.1016\/j.jprot.2026.105678_bb0100","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.ab.2015.04.002","article-title":"Single unit filter-aided method for fast proteomic analysis of tear fluid","volume":"480","author":"Aass","year":"2015","journal-title":"Anal. Biochem."},{"issue":"4","key":"10.1016\/j.jprot.2026.105678_bb0105","doi-asserted-by":"crossref","first-page":"1229","DOI":"10.1016\/j.survophthal.2022.01.010","article-title":"Metabolomics and lipidomics approaches in human tears: a systematic review","volume":"67","author":"Khanna","year":"2022","journal-title":"Surv. Ophthalmol."},{"issue":"1","key":"10.1016\/j.jprot.2026.105678_bb0110","doi-asserted-by":"crossref","first-page":"88","DOI":"10.4103\/tjo.tjo_14_21","article-title":"Effect of tear fluid sampling and processing on total protein quantity and electrophoretic pattern","volume":"12","author":"Kraj\u010d\u00edkov\u00e1","year":"2022","journal-title":"Taiwan J. Ophthalmol."},{"issue":"4","key":"10.1016\/j.jprot.2026.105678_bb0115","doi-asserted-by":"crossref","first-page":"1413","DOI":"10.4103\/IJO.IJO_2774_22","article-title":"Optimization and evaluation of tear protein elution from Schirmer\u2019s strips in dry eye disease","volume":"71","author":"Koduri","year":"2023","journal-title":"Indian J. Ophthalmol."},{"key":"10.1016\/j.jprot.2026.105678_bb0120","doi-asserted-by":"crossref","DOI":"10.1016\/j.preteyeres.2025.101338","article-title":"A comprehensive scoping review of methodological approaches and clinical applications of tear fluid biomarkers","volume":"106","author":"Gijs","year":"2025","journal-title":"Prog. Retin. Eye Res."},{"issue":"5","key":"10.1016\/j.jprot.2026.105678_bb0125","doi-asserted-by":"crossref","first-page":"2245","DOI":"10.1177\/1120672121998922","article-title":"Tear fluid collection methods: review of current techniques","volume":"31","author":"Pieczy\u0144ski","year":"2021","journal-title":"Eur. J. Ophthalmol."},{"issue":"2","key":"10.1016\/j.jprot.2026.105678_bb0130","doi-asserted-by":"crossref","first-page":"137","DOI":"10.1016\/j.aanat.2012.10.001","article-title":"Schirmer strip vs. capillary tube method: non-invasive methods of obtaining proteins from tear fluid","volume":"195","author":"Posa","year":"2013","journal-title":"Ann. Anat. = Anatom. Anzeiger\u202f: Off. Org. Anatom. Gesellsch."},{"issue":"5","key":"10.1016\/j.jprot.2026.105678_bb0135","doi-asserted-by":"crossref","first-page":"521","DOI":"10.1177\/0004563217695843","article-title":"Evaluation of commonly used tear sampling methods and their relevance in subsequent biochemical analysis","volume":"54","author":"Rentka","year":"2017","journal-title":"Ann. Clin. Biochem."},{"key":"10.1016\/j.jprot.2026.105678_bb0140","first-page":"456","article-title":"Investigation of the human tear film proteome using multiple proteomic approaches","volume":"14","author":"Green-Church","year":"2008","journal-title":"Mol. Vis."},{"issue":"1","key":"10.1016\/j.jprot.2026.105678_bb0145","doi-asserted-by":"crossref","DOI":"10.3390\/metabo12010002","article-title":"Proteomic analysis of tears and conjunctival cells collected with Schirmer strips using timsTOF pro: preanalytical considerations","volume":"12","author":"Akkurt Arslan","year":"2021","journal-title":"Metabolites"},{"key":"10.1016\/j.jprot.2026.105678_bb0150","doi-asserted-by":"crossref","DOI":"10.1186\/1477-5956-6-30","article-title":"Sample prep for proteomics of breast cancer: Proteomics and gene ontology reveal dramatic differences in protein solubilization preferences of radioimmunoprecipitation assay and urea lysis buffers","volume":"6","author":"Ngoka","year":"2008","journal-title":"Proteome Sci."},{"issue":"8","key":"10.1016\/j.jprot.2026.105678_bb0155","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0221052","article-title":"Evaluation of sodium deoxycholate as solubilization buffer for oil palm proteomics analysis","volume":"14","author":"Lau","year":"2019","journal-title":"PLoS One"},{"key":"10.1016\/j.jprot.2026.105678_bb0160","doi-asserted-by":"crossref","DOI":"10.1186\/1477-5956-7-38","article-title":"Optimized sample preparation for two-dimensional gel electrophoresis of soluble proteins from chicken bursa of Fabricius","volume":"7","author":"Wu","year":"2009","journal-title":"Proteome Sci."},{"issue":"5","key":"10.1016\/j.jprot.2026.105678_bb0165","doi-asserted-by":"crossref","first-page":"359","DOI":"10.1038\/nmeth.1322","article-title":"Universal sample preparation method for proteome analysis","volume":"6","author":"Wi\u015bniewski","year":"2009","journal-title":"Nat. Methods"},{"key":"10.1016\/j.jprot.2026.105678_bb0170","doi-asserted-by":"crossref","first-page":"283","DOI":"10.1016\/j.jtos.2025.05.005","article-title":"The ocular surface tear film as a biomarker for systemic health","volume":"37","author":"Fotovat-Ahmadi","year":"2025","journal-title":"Ocul. Surf."},{"key":"10.1016\/j.jprot.2026.105678_bb0175","doi-asserted-by":"crossref","first-page":"126","DOI":"10.1016\/j.exer.2013.07.015","article-title":"Tears as a source of biomarkers for ocular and systemic diseases","volume":"117","author":"von Thun Und Hohenstein-Blaul","year":"2013","journal-title":"Exp. Eye Res."},{"issue":"10","key":"10.1016\/j.jprot.2026.105678_bb0180","doi-asserted-by":"crossref","DOI":"10.3390\/ijms23105639","article-title":"Elevation of tear MMP-9 concentration as a biomarker of inflammation in ocular pathology by antibody microarray immunodetection assays","volume":"23","author":"de la Fuente","year":"2022","journal-title":"Int. J. Mol. Sci."},{"issue":"22","key":"10.1016\/j.jprot.2026.105678_bb0185","doi-asserted-by":"crossref","DOI":"10.3390\/ijms252212239","article-title":"High-throughput tear proteomics via in-capillary digestion for biomarker discovery","volume":"25","author":"Xiao","year":"2024","journal-title":"Int. J. Mol. Sci."}],"container-title":["Journal of Proteomics"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1874391926000813?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1874391926000813?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,7,4]],"date-time":"2026-07-04T08:57:24Z","timestamp":1783155444000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S1874391926000813"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,6]]},"references-count":37,"alternative-id":["S1874391926000813"],"URL":"https:\/\/doi.org\/10.1016\/j.jprot.2026.105678","relation":{},"ISSN":["1874-3919"],"issn-type":[{"value":"1874-3919","type":"print"}],"subject":[],"published":{"date-parts":[[2026,6]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Standardizing tear proteomics preanalytics: Capillary vs Schirmer sampling and denaturing vs mild elution buffers shape proteome depth and abundance profiles","name":"articletitle","label":"Article Title"},{"value":"Journal of Proteomics","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.jprot.2026.105678","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2026 The Authors. Published by Elsevier B.V.","name":"copyright","label":"Copyright"}],"article-number":"105678"}}