{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,2]],"date-time":"2026-09-02T12:18:02Z","timestamp":1788351482853,"version":"build-2803163510"},"reference-count":29,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T00:00:00Z","timestamp":1790812800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T00:00:00Z","timestamp":1790812800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T00:00:00Z","timestamp":1790812800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-017"},{"start":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T00:00:00Z","timestamp":1790812800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T00:00:00Z","timestamp":1790812800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-012"},{"start":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T00:00:00Z","timestamp":1790812800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T00:00:00Z","timestamp":1790812800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-004"}],"funder":[{"DOI":"10.13039\/501100008530","name":"European Regional Development Fund","doi-asserted-by":"publisher","award":["PID2021-125661NB-I00"],"award-info":[{"award-number":["PID2021-125661NB-I00"]}],"id":[{"id":"10.13039\/501100008530","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100025246","name":"Departament de Recerca i Universitats, Generalitat de Catalunya","doi-asserted-by":"publisher","award":["2021 SGR 00417"],"award-info":[{"award-number":["2021 SGR 00417"]}],"id":[{"id":"10.13039\/501100025246","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Journal of Proteomics"],"published-print":{"date-parts":[[2026,10]]},"DOI":"10.1016\/j.jprot.2026.105717","type":"journal-article","created":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T15:57:04Z","timestamp":1785427024000},"page":"105717","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":0,"special_numbering":"C","title":["Single-organ proteomics in Drosophila melanogaster larva"],"prefix":"10.1016","volume":"331","author":[{"given":"Ibon","family":"Iloro","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Coralia","family":"P\u00e9rez","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jabi","family":"Beaskoetxea","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mikel","family":"Azkargorta","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Iraide","family":"Escob\u00e9s","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David","family":"Mart\u00edn","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"James D.","family":"Sutherland","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rosa","family":"Barrio","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Felix","family":"Elortza","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"issue":"5461","key":"10.1016\/j.jprot.2026.105717_bb0005","doi-asserted-by":"crossref","first-page":"2185","DOI":"10.1126\/science.287.5461.2185","article-title":"The genome sequence of Drosophila melanogaster","volume":"287","author":"Adams","year":"2000","journal-title":"Science"},{"issue":"12","key":"10.1016\/j.jprot.2026.105717_bb0010","doi-asserted-by":"crossref","first-page":"1661","DOI":"10.1101\/gr.3726705","article-title":"Drosophila melanogaster: a case study of a model genomic sequence and its consequences","volume":"15","author":"Ashburner","year":"2005","journal-title":"Genome Res."},{"key":"10.1016\/j.jprot.2026.105717_bb0015","doi-asserted-by":"crossref","first-page":"393","DOI":"10.1038\/nature12962","article-title":"Diversity and dynamics of the Drosophila transcriptome","volume":"512","author":"Brown","year":"2014","journal-title":"Nature"},{"key":"10.1016\/j.jprot.2026.105717_bb0020","doi-asserted-by":"crossref","first-page":"402","DOI":"10.1038\/nature20802","article-title":"Genetic variants regulating expression levels and isoform diversity during embryogenesis","volume":"541","author":"Cannav\u00f2","year":"2017","journal-title":"Nature"},{"key":"10.1016\/j.jprot.2026.105717_bb0025","doi-asserted-by":"crossref","first-page":"576","DOI":"10.1038\/nbt1300","article-title":"A high-quality catalog of the Drosophila melanogaster proteome","volume":"25","author":"Brunner","year":"2007","journal-title":"Nat. Biotechnol."},{"issue":"2\u20133","key":"10.1016\/j.jprot.2026.105717_bb0030","doi-asserted-by":"crossref","first-page":"286","DOI":"10.1002\/pmic.201300121","article-title":"Qualitative and quantitative analysis of the adult Drosophila melanogaster proteome","volume":"14","author":"Xing","year":"2014","journal-title":"Proteomics"},{"issue":"2","key":"10.1016\/j.jprot.2026.105717_bb0035","doi-asserted-by":"crossref","first-page":"474","DOI":"10.1002\/pmic.200400923","article-title":"Proteomic analysis of the wing imaginal discs of Drosophila melanogaster","volume":"5","author":"Alonso","year":"2005","journal-title":"Proteomics"},{"issue":"9","key":"10.1016\/j.jprot.2026.105717_bb0040","doi-asserted-by":"crossref","first-page":"2484","DOI":"10.1002\/pmic.200800795","article-title":"Proteome mapping of the Drosophila melanogaster male reproductive system","volume":"9","author":"Takemori","year":"2009","journal-title":"Proteomics"},{"key":"10.1016\/j.jprot.2026.105717_bb0045","doi-asserted-by":"crossref","DOI":"10.1016\/j.jprot.2021.104307","article-title":"Mass spectrometric characterisation of the major peptides of the male ejaculatory duct, including a glycopeptide with an unusual zwitterionic glycosylation","volume":"246","author":"Sturm","year":"2021","journal-title":"J. Proteome"},{"issue":"4","key":"10.1016\/j.jprot.2026.105717_bb0050","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0018497","article-title":"A mighty small heart: the cardiac proteome of adult Drosophila melanogaster","volume":"6","author":"Cammarato","year":"2011","journal-title":"PLoS One"},{"issue":"10","key":"10.1016\/j.jprot.2026.105717_bb0055","doi-asserted-by":"crossref","DOI":"10.1002\/pmic.202300330","article-title":"Eye proteome of Drosophila melanogaster","volume":"24","author":"Kumar","year":"2024","journal-title":"Proteomics"},{"issue":"1","key":"10.1016\/j.jprot.2026.105717_bb0060","doi-asserted-by":"crossref","first-page":"71","DOI":"10.1134\/S0006297919010097","article-title":"Brain proteome of Drosophila melanogaster is enriched with nuclear proteins brain proteome of Drosophila melanogaster is enriched with nuclear proteins","volume":"84","author":"Kuznetsova","year":"2019","journal-title":"Biochemistry (Mosc)"},{"issue":"6","key":"10.1016\/j.jprot.2026.105717_bb0065","first-page":"369","article-title":"Global proteomic profiling of Drosophila ovary: a high-resolution, unbiased, accurate and multifaceted analysis","volume":"12","author":"Velentzas","year":"2015","journal-title":"Cancer Genomics Proteomics"},{"issue":"Suppl. 1","key":"10.1016\/j.jprot.2026.105717_bb0070","doi-asserted-by":"crossref","first-page":"S23","DOI":"10.1074\/mcp.RA118.001098","article-title":"The old and the new: discovery proteomics identifies putative novel seminal fluid proteins in Drosophila","volume":"18","author":"Karr","year":"2019","journal-title":"Mol. Cell. Proteomics"},{"issue":"11","key":"10.1016\/j.jprot.2026.105717_bb0075","doi-asserted-by":"crossref","first-page":"1687","DOI":"10.3390\/biology11111687","article-title":"Proteomic characterization of Drosophila melanogaster proboscis","volume":"11","author":"Aru\u00e7i","year":"2022","journal-title":"Biol.-Basel"},{"key":"10.1016\/j.jprot.2026.105717_bib136","doi-asserted-by":"crossref","first-page":"667","DOI":"10.1126\/science.1119432","article-title":"Antagonistic actions of ecdysone and insulins determine final size in Drosophila","volume":"310","author":"Colombani","year":"2005","journal-title":"Science"},{"issue":"20","key":"10.1016\/j.jprot.2026.105717_bib137","doi-asserted-by":"crossref","first-page":"1796","DOI":"10.1016\/j.cub.2005.09.017","article-title":"The role of the prothoracic gland in determining critical weight for metamorphosis in Drosophila melanogaster","volume":"15","author":"Mirth","year":"2005","journal-title":"Curr. Biol."},{"issue":"11","key":"10.1016\/j.jprot.2026.105717_bb0080","doi-asserted-by":"crossref","first-page":"2284","DOI":"10.1074\/mcp.TIR118.000853","article-title":"A novel LC system embeds analytes in pre-formed gradients for rapid, ultra-robust proteomics","volume":"17","author":"Bache","year":"2018","journal-title":"Mol. Cell. Proteomics"},{"issue":"12","key":"10.1016\/j.jprot.2026.105717_bb0085","doi-asserted-by":"crossref","first-page":"5378","DOI":"10.1021\/acs.jproteome.5b00932","article-title":"Parallel accumulation-serial fragmentation (PASEF): multiplying sequencing speed and sensitivity by synchronized scans in a trapped ion mobility device","volume":"14","author":"Meier","year":"2015","journal-title":"J. Proteome Res."},{"key":"10.1016\/j.jprot.2026.105717_bb0090","doi-asserted-by":"crossref","first-page":"15","DOI":"10.1007\/978-1-0716-2124-0_2","article-title":"High-throughput mass spectrometry-based proteomics with dia-PASEF","volume":"2456","author":"Skowronek","year":"2022","journal-title":"Methods Mol. Biol."},{"issue":"1","key":"10.1016\/j.jprot.2026.105717_bb0095","doi-asserted-by":"crossref","first-page":"41","DOI":"10.1038\/s41592-019-0638-x","article-title":"DIA-NN: neural networks and interference correction enable deep proteome coverage in high throughput","volume":"17","author":"Demichev","year":"2020","journal-title":"Nat. Methods"},{"issue":"1","key":"10.1016\/j.jprot.2026.105717_bb0100","doi-asserted-by":"crossref","first-page":"2474","DOI":"10.1038\/s41467-024-46777-9","article-title":"One-tip enables comprehensive proteome coverage in minimal cells and single zygotes","volume":"15","author":"Ye","year":"2024","journal-title":"Nat. Commun."},{"issue":"7","key":"10.1016\/j.jprot.2026.105717_bb0105","article-title":"Freeze-thaw lysis for extraction of proteins from mammalian cells","volume":"2006","author":"Tansey","year":"2006","journal-title":"CSH Protoc."},{"key":"10.1016\/j.jprot.2026.105717_bb0110","doi-asserted-by":"crossref","DOI":"10.1016\/j.ab.2022.114924","article-title":"Maximizing hydrophobic peptide recovery in proteomics and antibody development using a mass spectrometry compatible surfactant","volume":"658","author":"Nie","year":"2022","journal-title":"Anal. Biochem."},{"issue":"8","key":"10.1016\/j.jprot.2026.105717_bb0115","doi-asserted-by":"crossref","first-page":"3294","DOI":"10.1021\/acs.jproteome.3c00862","article-title":"A rapid one-pot workflow for sensitive microscale phosphoproteomics","volume":"23","author":"Muneer","year":"2024","journal-title":"J. Proteome Res."},{"issue":"6","key":"10.1016\/j.jprot.2026.105717_bb0120","doi-asserted-by":"crossref","first-page":"1105","DOI":"10.1016\/j.biocel.2007.01.007","article-title":"Transdetermination: Drosophila imaginal disc cells exhibit stem cell-like potency","volume":"39","author":"McClure","year":"2007","journal-title":"Int. J. Biochem. Cell Biol."},{"issue":"4","key":"10.1016\/j.jprot.2026.105717_bb0125","doi-asserted-by":"crossref","DOI":"10.1093\/genetics\/iyac020","article-title":"The wing imaginal disc","volume":"220","author":"Tripathi","year":"2022","journal-title":"Genetics"},{"issue":"2","key":"10.1016\/j.jprot.2026.105717_bb0130","doi-asserted-by":"crossref","first-page":"467","DOI":"10.1534\/g3.116.037333","article-title":"Transcriptome analysis of Drosophila melanogaster third instar larval ring glands points to novel functions and uncovers a cytochrome p450 required for development","volume":"7","author":"Christesen","year":"2017","journal-title":"G3 (Bethesda)"},{"key":"10.1016\/j.jprot.2026.105717_bb0135","article-title":"The Drosophila larval salivary gland, a simple and elegant model system to understand secretory organ development and function","volume":"73","author":"Kim","year":"2026","journal-title":"Curr. Opin. Insect. Sci."}],"container-title":["Journal of Proteomics"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S187439192600120X?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S187439192600120X?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,9,2]],"date-time":"2026-09-02T11:40:12Z","timestamp":1788349212000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S187439192600120X"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,10]]},"references-count":29,"alternative-id":["S187439192600120X"],"URL":"https:\/\/doi.org\/10.1016\/j.jprot.2026.105717","relation":{},"ISSN":["1874-3919"],"issn-type":[{"value":"1874-3919","type":"print"}],"subject":[],"published":{"date-parts":[[2026,10]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Single-organ proteomics in Drosophila melanogaster larva","name":"articletitle","label":"Article Title"},{"value":"Journal of Proteomics","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.jprot.2026.105717","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2026 Elsevier B.V. All rights are reserved, including those for text and data mining, AI training, and similar technologies.","name":"copyright","label":"Copyright"}],"article-number":"105717"}}