{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T18:17:19Z","timestamp":1790792239576,"version":"4.1.0"},"reference-count":40,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2023,12,1]],"date-time":"2023-12-01T00:00:00Z","timestamp":1701388800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2023,12,1]],"date-time":"2023-12-01T00:00:00Z","timestamp":1701388800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2023,12,1]],"date-time":"2023-12-01T00:00:00Z","timestamp":1701388800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-017"},{"start":{"date-parts":[[2023,12,1]],"date-time":"2023-12-01T00:00:00Z","timestamp":1701388800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2023,12,1]],"date-time":"2023-12-01T00:00:00Z","timestamp":1701388800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-012"},{"start":{"date-parts":[[2023,12,1]],"date-time":"2023-12-01T00:00:00Z","timestamp":1701388800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2023,12,1]],"date-time":"2023-12-01T00:00:00Z","timestamp":1701388800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-004"}],"funder":[{"DOI":"10.13039\/501100007613","name":"Majmaah University","doi-asserted-by":"publisher","award":["R-2023-664"],"award-info":[{"award-number":["R-2023-664"]}],"id":[{"id":"10.13039\/501100007613","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["clinicalkey.com","clinicalkey.com.au","clinicalkey.es","clinicalkey.fr","clinicalkey.jp","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Journal of Reproductive Immunology"],"published-print":{"date-parts":[[2023,12]]},"DOI":"10.1016\/j.jri.2023.104159","type":"journal-article","created":{"date-parts":[[2023,10,3]],"date-time":"2023-10-03T01:55:16Z","timestamp":1696298116000},"page":"104159","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":6,"special_numbering":"C","title":["Reduced expression of miR-221 is associated with the pro-apoptotic pathways in spermatozoa of oligospermia men"],"prefix":"10.1016","volume":"160","author":[{"given":"Mohamed J.","family":"Saadh","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Renzon Daniel Cosme","family":"Pecho","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Azfar","family":"Jamal","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Abdulaziz S.","family":"Alothaim","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohammad Azhar","family":"Kamal","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohiuddin Khan","family":"Warsi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fuzail","family":"Ahmad","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohammed","family":"Obaid","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hani","family":"Moslem","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H.A.","family":"Zainab","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ali H.","family":"Amin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jos\u00e9 Luis","family":"Arias-Gonz\u00e1les","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0009-7820-5552","authenticated-orcid":false,"given":"Ria","family":"Margiana","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Reza","family":"Akhavan-Sigari","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.jri.2023.104159_bib1","doi-asserted-by":"crossref","first-page":"4","DOI":"10.4103\/jhrs.JHRS_150_18","article-title":"Role of oxidative stress in male infertility: an updated review","volume":"12","author":"Alahmar","year":"2019","journal-title":"J. Hum. Reprod. Sci."},{"key":"10.1016\/j.jri.2023.104159_bib2","first-page":"88","article-title":"Genetic risk of azoospermia factor (azf) microdeletions in idiopathic cases of azoospermia and oligozoospermia in central indian population","volume":"8","author":"Ambulkar","year":"2014","journal-title":"J. Clin. Diagn. Res.: JCDR"},{"key":"10.1016\/j.jri.2023.104159_bib3","doi-asserted-by":"crossref","first-page":"115","DOI":"10.1038\/sj.cgt.7700896","article-title":"The transcription factor egr1 is a direct regulator of multiple tumor suppressors including tgf\u03b21, pten, p53, and fibronectin","volume":"13","author":"Baron","year":"2006","journal-title":"Cancer Gene Ther."},{"key":"10.1016\/j.jri.2023.104159_bib4","doi-asserted-by":"crossref","unstructured":"Boitrelle, F., et al., 2021. The sixth edition of the who manual for human semen analysis: A critical review and swot analysis. Life (Basel, Switzerland). 11.","DOI":"10.3390\/life11121368"},{"key":"10.1016\/j.jri.2023.104159_bib5","doi-asserted-by":"crossref","first-page":"919","DOI":"10.1007\/s12033-022-00473-5","article-title":"Mir-221-3p facilitates thyroid cancer cell proliferation and inhibit apoptosis by targeting foxp2 through hedgehog pathway","volume":"64","author":"Chang","year":"2022","journal-title":"Mol. Biotechnol."},{"key":"10.1016\/j.jri.2023.104159_bib6","article-title":"Oxidative stress and immunological biomarkers in ankylosing spondylitis patients","volume":"18","author":"Danaii","year":"2020","journal-title":"Gene Rep."},{"key":"10.1016\/j.jri.2023.104159_bib7","doi-asserted-by":"crossref","first-page":"763","DOI":"10.1007\/s10815-019-01674-9","article-title":"Micrornas association with azoospermia, oligospermia, asthenozoospermia, and teratozoospermia: a systematic review","volume":"37","author":"Daneshmandpour","year":"2020","journal-title":"J. Assist. Reprod. Genet."},{"key":"10.1016\/j.jri.2023.104159_bib8","doi-asserted-by":"crossref","first-page":"349","DOI":"10.2164\/jandrol.04146","article-title":"Reduction of the incidence of sperm DNA fragmentation by oral antioxidant treatment","volume":"26","author":"Greco","year":"2005","journal-title":"J. Androl."},{"key":"10.1016\/j.jri.2023.104159_bib9","doi-asserted-by":"crossref","first-page":"a008722","DOI":"10.1101\/cshperspect.a008722","article-title":"Multiple functions of bcl-2 family proteins","volume":"5","author":"Hardwick","year":"2013","journal-title":"Cold Spring Harb. Perspect. Biol."},{"key":"10.1016\/j.jri.2023.104159_bib10","doi-asserted-by":"crossref","first-page":"1369","DOI":"10.4155\/fmc-2017-0246","article-title":"Recent advances in cdk inhibitors for cancer therapy","volume":"10","author":"Heptinstall","year":"2018","journal-title":"Future Med. Chem."},{"key":"10.1016\/j.jri.2023.104159_bib11","doi-asserted-by":"crossref","first-page":"205","DOI":"10.15171\/apb.2019.024","article-title":"Understanding apoptosis and apoptotic pathways targeted cancer therapeutics","volume":"9","author":"Jan","year":"2019","journal-title":"Adv. Pharm. Bull."},{"key":"10.1016\/j.jri.2023.104159_bib12","doi-asserted-by":"crossref","first-page":"2416","DOI":"10.1002\/jcb.29464","article-title":"Docosahexaenoic acid suppresses migration of triple\u2010negative breast cancer cell through targeting metastasis\u2010related genes and microrna under normoxic and hypoxic conditions","volume":"121","author":"Javadian","year":"2020","journal-title":"J. Cell. Biochem."},{"key":"10.1016\/j.jri.2023.104159_bib13","doi-asserted-by":"crossref","unstructured":"Kanatsu-Shinohara, M., et al., 2010. Transmission distortion by loss of p21 or p27 cyclin-dependent kinase inhibitors following competitive spermatogonial transplantation. Proceedings of the National Academy of Sciences. 107, pp. 6210\u20136215.","DOI":"10.1073\/pnas.0914448107"},{"key":"10.1016\/j.jri.2023.104159_bib14","doi-asserted-by":"crossref","first-page":"5526","DOI":"10.3390\/ijms20225526","article-title":"Aeg-1\/mir-221 axis cooperatively regulates the progression of hepatocellular carcinoma by targeting pten\/pi3k\/akt signaling pathway","volume":"20","author":"Kannan","year":"2019","journal-title":"Int. J. Mol. Sci."},{"key":"10.1016\/j.jri.2023.104159_bib15","first-page":"1801","article-title":"Downregulation of long non\u2011coding rna gas5 promotes cell proliferation, migration and invasion in esophageal squamous cell carcinoma","volume":"16","author":"Ke","year":"2018","journal-title":"Oncol. Lett."},{"key":"10.1016\/j.jri.2023.104159_bib16","doi-asserted-by":"crossref","first-page":"263","DOI":"10.1017\/S0967199419000340","article-title":"Microrna expression in infertile men: Its alterations and effects","volume":"27","author":"Kiani","year":"2019","journal-title":"Zygote"},{"key":"10.1016\/j.jri.2023.104159_bib17","doi-asserted-by":"crossref","first-page":"1087","DOI":"10.3892\/ijo.2017.3898","article-title":"Overexpression of mirna-221 promotes cell proliferation by targeting the apoptotic protease activating factor-1 and indicates a poor prognosis in ovarian cancer","volume":"50","author":"Li","year":"2017","journal-title":"Int. J. Oncol."},{"key":"10.1016\/j.jri.2023.104159_bib18","doi-asserted-by":"crossref","first-page":"13","DOI":"10.1186\/1477-7827-7-13","article-title":"Altered microrna expression in patients with non-obstructive azoospermia","volume":"7","author":"Lian","year":"2009","journal-title":"Reprod. Biol. Endocrinol.: RBE"},{"key":"10.1016\/j.jri.2023.104159_bib19","first-page":"3193","article-title":"Inhibition of mir-221 influences bladder cancer cell proliferation and apoptosis","volume":"21","author":"Liu","year":"2017","journal-title":"Eur. Rev. Med. Pharmacol. Sci."},{"key":"10.1016\/j.jri.2023.104159_bib20","doi-asserted-by":"crossref","first-page":"1097","DOI":"10.4252\/wjsc.v12.i10.1097","article-title":"Stem cell-based therapies for fertility preservation in males: Current status and future prospects","volume":"12","author":"Liu","year":"2020","journal-title":"World J. Stem Cells"},{"key":"10.1016\/j.jri.2023.104159_bib21","doi-asserted-by":"crossref","first-page":"2935","DOI":"10.3390\/ijms22062935","article-title":"Cyclin-dependent kinases (cdk) and their role in diseases development\u2013review","volume":"22","author":"\u0141ukasik","year":"2021","journal-title":"Int. J. Mol. Sci."},{"key":"10.1016\/j.jri.2023.104159_bib22","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pgen.1004954","article-title":"Polycomb protein scml2 associates with usp7 and counteracts histone h2a ubiquitination in the xy chromatin during male meiosis","volume":"11","author":"Luo","year":"2015","journal-title":"PLoS Genet."},{"key":"10.1016\/j.jri.2023.104159_bib23","doi-asserted-by":"crossref","first-page":"1233","DOI":"10.3390\/ijms19041233","article-title":"Testosterone-dependent mir-26a-5p and let-7g-5p act as signaling mediators to regulate sperm apoptosis via targeting pten and pmaip1","volume":"19","author":"Ma","year":"2018","journal-title":"Int. J. Mol. Sci."},{"key":"10.1016\/j.jri.2023.104159_bib24","doi-asserted-by":"crossref","first-page":"814","DOI":"10.1016\/j.fertnstert.2008.10.068","article-title":"Sperm viability, apoptosis, and intracellular reactive oxygen species levels in human spermatozoa before and after induction of oxidative stress","volume":"93","author":"Mahfouz","year":"2010","journal-title":"Fertil. Steril."},{"key":"10.1016\/j.jri.2023.104159_bib25","doi-asserted-by":"crossref","first-page":"S649","DOI":"10.21037\/tau.2017.07.09","article-title":"Antioxidants for elevated sperm DNA fragmentation: a mini review","volume":"6","author":"Majzoub","year":"2017","journal-title":"Transl. Androl. Urol."},{"key":"10.1016\/j.jri.2023.104159_bib26","doi-asserted-by":"crossref","first-page":"121","DOI":"10.2174\/187231207780363633","article-title":"The human sperm glutathione system: a key role in male fertility and successful cryopreservation","volume":"1","author":"Meseguer","year":"2007","journal-title":"Drug Metab. Lett."},{"key":"10.1016\/j.jri.2023.104159_bib27","doi-asserted-by":"crossref","first-page":"281","DOI":"10.1007\/s10456-018-9656-z","article-title":"Tspyl5-mediated inhibition of p53 promotes human endothelial cell function","volume":"22","author":"Na","year":"2019","journal-title":"Angiogenesis"},{"key":"10.1016\/j.jri.2023.104159_bib28","doi-asserted-by":"crossref","first-page":"134","DOI":"10.3390\/antiox9020134","article-title":"Molecular changes induced by oxidative stress that impair human sperm motility","volume":"9","author":"Nowicka-Bauer","year":"2020","journal-title":"Antioxidants"},{"key":"10.1016\/j.jri.2023.104159_bib29","doi-asserted-by":"crossref","unstructured":"Ojo, O.A., et al., 2023. Apoptosis, inflammation, and oxidative stress in infertility: A mini review. Toxicology Reports.","DOI":"10.1016\/j.toxrep.2023.04.006"},{"key":"10.1016\/j.jri.2023.104159_bib30","doi-asserted-by":"crossref","first-page":"138","DOI":"10.1111\/j.1365-2230.2005.01925.x","article-title":"Gynaecomastia with oligospermia: an unusual complication of low\u2010dose methotrexate for pustular psoriasis","volume":"31","author":"Pandhi","year":"2006","journal-title":"Clin. Exp. Dermatol."},{"key":"10.1016\/j.jri.2023.104159_bib31","doi-asserted-by":"crossref","first-page":"19","DOI":"10.1007\/s10815-022-02674-y","article-title":"Modeling methods for busulfan-induced oligospermia and asthenozoospermia in mice: a systematic review and meta-analysis","volume":"40","author":"Pu","year":"2023","journal-title":"J. Assist. Reprod. Genet."},{"key":"10.1016\/j.jri.2023.104159_bib32","doi-asserted-by":"crossref","first-page":"51","DOI":"10.2147\/TACG.S267421","article-title":"Y-chromosome microdeletions: A review of prevalence, screening, and clinical considerations","author":"Rabinowitz","year":"2021","journal-title":"Appl. Clin. Genet."},{"key":"10.1016\/j.jri.2023.104159_bib33","doi-asserted-by":"crossref","first-page":"78","DOI":"10.3390\/antiox9010078","article-title":"Catalase as a molecular target for male infertility diagnosis and monitoring: an overview","volume":"9","author":"Rubio-Riquelme","year":"2020","journal-title":"Antioxidants"},{"key":"10.1016\/j.jri.2023.104159_bib34","doi-asserted-by":"crossref","first-page":"4984","DOI":"10.1096\/fj.201701096RR","article-title":"Cep55 overexpression causes male\u2010specific sterility in mice by suppressing foxo1 nuclear retention through sustained activation of pi3k\/akt signaling","volume":"32","author":"Sinha","year":"2018","journal-title":"FASEB J."},{"key":"10.1016\/j.jri.2023.104159_bib35","doi-asserted-by":"crossref","first-page":"283","DOI":"10.1038\/nrm3330","article-title":"The functions and regulation of the pten tumour suppressor","volume":"13","author":"Song","year":"2012","journal-title":"Nat. Rev. Mol. Cell Biol."},{"key":"10.1016\/j.jri.2023.104159_bib36","doi-asserted-by":"crossref","first-page":"286","DOI":"10.3389\/fgene.2012.00286","article-title":"Egfr signaling pathway and related-mirnas in age-related diseases: The example of mir-221 and mir-222","volume":"3","author":"Teixeira","year":"2012","journal-title":"Front. Genet."},{"key":"10.1016\/j.jri.2023.104159_bib37","first-page":"6697","article-title":"Mir-221 regulates proliferation and apoptosis of ovarian cancer cells by targeting bmf","volume":"16","author":"Xie","year":"2018","journal-title":"Oncol. Lett."},{"key":"10.1016\/j.jri.2023.104159_bib38","doi-asserted-by":"crossref","first-page":"47","DOI":"10.4103\/0974-1208.178629","article-title":"Antisperm protein targets in azoospermia men","volume":"9","author":"Zangbar","year":"2016","journal-title":"J. Hum. Reprod. Sci."},{"key":"10.1016\/j.jri.2023.104159_bib39","doi-asserted-by":"crossref","first-page":"410","DOI":"10.1016\/j.freeradbiomed.2021.01.014","article-title":"Melatonin alleviates heat stress-induced oxidative stress and apoptosis in human spermatozoa","volume":"164","author":"Zhao","year":"2021","journal-title":"Free Radic. Biol. Med."},{"key":"10.1016\/j.jri.2023.104159_bib40","first-page":"39","article-title":"Mir-221\/222 promote tumor growth and suppress apoptosis by targeting lncrna gas5 in breast cancer","author":"Zong","year":"2019","journal-title":"Biosci. Rep."}],"container-title":["Journal of Reproductive Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0165037823003650?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0165037823003650?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,9,28]],"date-time":"2025-09-28T04:36:23Z","timestamp":1759034183000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0165037823003650"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,12]]},"references-count":40,"alternative-id":["S0165037823003650"],"URL":"https:\/\/doi.org\/10.1016\/j.jri.2023.104159","relation":{},"ISSN":["0165-0378"],"issn-type":[{"value":"0165-0378","type":"print"}],"subject":[],"published":{"date-parts":[[2023,12]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Reduced expression of miR-221 is associated with the pro-apoptotic pathways in spermatozoa of oligospermia men","name":"articletitle","label":"Article Title"},{"value":"Journal of Reproductive Immunology","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.jri.2023.104159","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2023 Published by Elsevier B.V.","name":"copyright","label":"Copyright"}],"article-number":"104159"}}